Title: Regulation of p53 activity in nuclear bodies by a specific PML isoform
Abstract: Article15 November 2000free access Regulation of p53 activity in nuclear bodies by a specific PML isoform Valentina Fogal Valentina Fogal Laboratorio Nazionale CIB, Area Science Park, Padriciano 99, 34012 Trieste, Italy V.Fogal and M.Gostissa contributed equally to this work Search for more papers by this author Monica Gostissa Monica Gostissa Laboratorio Nazionale CIB, Area Science Park, Padriciano 99, 34012 Trieste, Italy V.Fogal and M.Gostissa contributed equally to this work Search for more papers by this author Peter Sandy Peter Sandy Laboratorio Nazionale CIB, Area Science Park, Padriciano 99, 34012 Trieste, Italy Search for more papers by this author Paola Zacchi Paola Zacchi Laboratorio Nazionale CIB, Area Science Park, Padriciano 99, 34012 Trieste, Italy Search for more papers by this author Thomas Sternsdorf Thomas Sternsdorf Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Martinstrasse 52, D-20251 Hamburg, Germany Search for more papers by this author Kirsten Jensen Kirsten Jensen Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Martinstrasse 52, D-20251 Hamburg, Germany Search for more papers by this author Pier Paolo Pandolfi Pier Paolo Pandolfi Department of Human Genetics and Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, 10021 USA Search for more papers by this author Hans Will Hans Will Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Martinstrasse 52, D-20251 Hamburg, Germany Search for more papers by this author Claudio Schneider Claudio Schneider Laboratorio Nazionale CIB, Area Science Park, Padriciano 99, 34012 Trieste, Italy Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine, Piazzale Kolbe 4, 33100 Udine, Italy Search for more papers by this author Giannino Del Sal Corresponding Author Giannino Del Sal Laboratorio Nazionale CIB, Area Science Park, Padriciano 99, 34012 Trieste, Italy Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università di Trieste, Via L. Giorgeri 1, 34100 Trieste, Italy Search for more papers by this author Valentina Fogal Valentina Fogal Laboratorio Nazionale CIB, Area Science Park, Padriciano 99, 34012 Trieste, Italy V.Fogal and M.Gostissa contributed equally to this work Search for more papers by this author Monica Gostissa Monica Gostissa Laboratorio Nazionale CIB, Area Science Park, Padriciano 99, 34012 Trieste, Italy V.Fogal and M.Gostissa contributed equally to this work Search for more papers by this author Peter Sandy Peter Sandy Laboratorio Nazionale CIB, Area Science Park, Padriciano 99, 34012 Trieste, Italy Search for more papers by this author Paola Zacchi Paola Zacchi Laboratorio Nazionale CIB, Area Science Park, Padriciano 99, 34012 Trieste, Italy Search for more papers by this author Thomas Sternsdorf Thomas Sternsdorf Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Martinstrasse 52, D-20251 Hamburg, Germany Search for more papers by this author Kirsten Jensen Kirsten Jensen Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Martinstrasse 52, D-20251 Hamburg, Germany Search for more papers by this author Pier Paolo Pandolfi Pier Paolo Pandolfi Department of Human Genetics and Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, 10021 USA Search for more papers by this author Hans Will Hans Will Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Martinstrasse 52, D-20251 Hamburg, Germany Search for more papers by this author Claudio Schneider Claudio Schneider Laboratorio Nazionale CIB, Area Science Park, Padriciano 99, 34012 Trieste, Italy Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine, Piazzale Kolbe 4, 33100 Udine, Italy Search for more papers by this author Giannino Del Sal Corresponding Author Giannino Del Sal Laboratorio Nazionale CIB, Area Science Park, Padriciano 99, 34012 Trieste, Italy Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università di Trieste, Via L. Giorgeri 1, 34100 Trieste, Italy Search for more papers by this author Author Information Valentina Fogal1,6, Monica Gostissa1,6, Peter Sandy1, Paola Zacchi1, Thomas Sternsdorf2, Kirsten Jensen2, Pier Paolo Pandolfi3, Hans Will2, Claudio Schneider1,4 and Giannino Del Sal 1,5 1Laboratorio Nazionale CIB, Area Science Park, Padriciano 99, 34012 Trieste, Italy 2Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Martinstrasse 52, D-20251 Hamburg, Germany 3Department of Human Genetics and Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, 10021 USA 4Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine, Piazzale Kolbe 4, 33100 Udine, Italy 5Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università di Trieste, Via L. Giorgeri 1, 34100 Trieste, Italy 6V.Fogal and M.Gostissa contributed equally to this work *Corresponding author. E-mail: [email protected] The EMBO Journal (2000)19:6185-6195https://doi.org/10.1093/emboj/19.22.6185 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Covalent modification of the promyelocytic leukaemia protein (PML) by SUMO-1 is a prerequisite for the assembly of nuclear bodies (NBs), subnuclear structures disrupted in various human diseases and linked to transcriptional and growth control. Here we demonstrate that p53 is recruited into NBs by a specific PML isoform (PML3) or by coexpression of SUMO-1 and hUbc9. NB targeting depends on the direct association of p53, through its core domain, with a C-terminal region of PML3. The relocalization of p53 into NBs enhances p53 transactivation in a promoter-specific manner and affects cell survival. Our results indicate the existence of a cross-talk between PML- and p53-dependent growth suppression pathways, implying an important role for NBs and their resident proteins as modulators of p53 functions. Introduction The tumour suppressor protein p53 is a key element in the control of human cell growth and differentiation and plays an important role in the maintenance of genome integrity (Ko and Prives, 1996; Levine, 1997). Most of its functions are exerted by transcriptional activation of genes involved in cell cycle, apoptosis and DNA repair (Ko and Prives, 1996). Under various stress conditions p53 becomes activated by post-translational modifications that affect its conformation and binding to several proteins, resulting in its stabilization and increased DNA-binding potential (Giaccia and Kastan, 1998). Another way to modulate p53 activity involves changes in its subcellular distribution. Certain tumours constitutively accumulate wild-type p53, which is functionally inactive because it is sequestered in the cytoplasm (Moll et al., 1996; Ostermayer et al., 1996). In addition, treatment of human primary cells with Leptomycin B, a drug that specifically blocks nuclear export, induces the relocalization of p53 into punctate subnuclear structures, reminiscent of the so-called nuclear bodies (NBs) (Lain et al., 1999). A similar p53 distribution has also been observed upon coexpression with Mdm2 and ARF and this relocalization has been correlated with ARF-mediated inhibition of Mdm2–p53 nuclear export (Zhang and Xiong, 1999). NBs are cell cycle-regulated, matrix-associated subnuclear structures that appear as punctate foci in the interphase nuclei (Seeler and Dejean, 1999). The structural integrity of these large multiprotein complexes appears to be important for normal cell growth and development, since in some human diseases, like acute promyelocytic leukaemia (APL) and spinocerebellar ataxia type I (SCA1), disruption of NBs leads to malignancy or neurodegenerative disorder, respectively (Hodges et al., 1998). Moreover, these structures are targeted and subsequently destroyed by numerous immediate early viral proteins (Maul, 1998). Promyelocytic leukaemia protein (PML), the most prominent component of the NBs (also referred to as PML oncogenic domains, PODs), was first identified in APL patients, where, as a result of a reciprocal translocation event, it is fused to the retinoic acid receptor α (RARα) (de The et al., 1991; Kakizuka et al., 1991). The fundamental role of PML in directing the complex protein–protein interactions that mediate PODs formation is underlined by recent findings that the organization of several NB-associated components is impaired in PML−/− cells (Zhong et al., 2000a). The possible role of PML and NBs in control of cell growth is suggested by studies on PML knockout mice, revealing tumour suppressor and pro-apoptotic functions for PML (Quignon et al., 1998; Wang et al., 1998b). In APL cells, the expression of the PML–RARα fusion compromises the integrity of PODs, while treatment with therapeutic agents, such as arsenic trioxide (As2O3) or interferons, leads to the normalization of NB pattern and simultaneously induces differentiation or apoptosis of the malignant cells (Lavau et al., 1995; Muller et al., 1998b). Other evidence implies NBs in the control of gene expression (Zhong et al., 2000b). The reported interaction with pRb (Alcalay et al., 1998) and the direct binding to the histone acetyltransferase CBP (LaMorte et al., 1998) suggest a relevant role for PML in the regulation of transcription. PML, Sp100 and probably other NB-resident proteins are post-translationally modified by SUMO-1, a small ubiquitin-related modifier (Sternsdorf et al., 1997), and recently we and others reported that p53 is also conjugated to SUMO-1 (Gostissa et al., 1999; Rodriguez et al., 1999; Muller et al., 2000). It has been demonstrated that sumolation of PML is absolutely required for NB formation and for recruitment of other factors to these structures (Ishov et al., 1999; Zhong et al., 2000a). Here we provide evidence that PML recruits p53 into NBs. The relocalization of p53 depends on its direct association with a specific PML splice variant, PML3. Moreover, we demonstrate that binding to PML3 and NB targeting of p53 result in increased transcriptional activation of a p53-regulated pro-apoptotic gene and affect cell survival. Results PML mediates the relocalization of p53 into NBs Recently we and others reported that p53 can be covalently modified by conjugation to the small ubiquitin-like protein SUMO-1 (Gostissa et al., 1999; Rodriguez et al., 1999; Muller et al., 2000) and we noticed that upon coexpression of SUMO-1 and hUbc9, p53 was relocalized to subnuclear structures reminiscent of NBs. Since SUMO-1 conjugation has been proposed to modulate the subcellular localization of several proteins (Mahajan et al., 1997), we wanted to investigate whether it may assist the relocalization of p53 into NBs. Human p53-null SaOS-2 cells were microinjected with plasmids encoding wild-type p53 (p53 wt), green fluorescent protein (GFP)–SUMO-1 and haemagglutinin (HA)-hUbc9, and analysed by immunofluorescence and confocal laser microscopy. In a fraction of the injected cells (40%) the typical nucleoplasmic staining of p53 (Figure 1A, a) became organized in distinct GFP–SUMO-1-positive NBs (Figure 1A, b–d). To our surprise, the conjugation-deficient mutant p53 K386R, when microinjected in the same conditions, was relocalized to NBs to a similar extent to the wt protein (Figure 1A, f–h), thus demonstrating that sumolation of p53 is dispensable for its delivery to these structures. Notably, a similar independence of NB localization from SUMO-1 conjugation has also been described for Sp100 (Sternsdorf et al., 1999) and the transactivator protein IE2-p86 of human cytomegalovirus (Hofmann et al., 2000). NB targeting was p53 specific, since under the same conditions a construct encoding β-galactosidase fused to a nuclear localization signal (βgal-NLS) was excluded from these structures (Figure 1A, i–k). Moreover, the observed p53 relocalization was dependent on the simultaneous expression of both GFP–SUMO-1 and HA-hUbc9 (not shown). Of note, when expressed individually, HA-hUbc9 revealed both nuclear and cytoplasmic distribution, while GFP–SUMO-1 showed a nuclear diffuse staining with the protein concentrating in small dots (not shown). Coexpression of the two proteins, instead, resulted in a dramatic change of subcellular distribution and in the formation of large NBs (Figure 1A, c, g and j) where the two proteins colocalized (not shown), suggesting that both factors are rate-limiting in a process that led to the formation of these structures. Figure 1.p53 relocalizes into NBs. (A) SaOS-2 cells were microinjected with either 10 ng/μl pcDNA3p53wt (b–d), pRcCMVp53K386R (f–h) or pNLS-βgal (i–k) together with 30 ng/μl pGFPSUMO-1 and 50 ng/μl pcDNA3HAhUbc9. p53 staining was analysed using a rabbit antiserum, while the localization of βgal-NLS was detected by a monoclonal anti-β-galactosidase antibody. Primary antibodies and GFP–SUMO-1 staining were revealed by incubation with TRITC-conjugated secondary antibodies or by the intrinsic green fluorescence of GFP, respectively. Merging of the two colours results in a yellow signal, corresponding to colocalized proteins. (a and e) Staining of p53 wt and K386R, respectively, in the absence of coexpressed GFP–SUMO-1 and HA-hUbc9. (B) SaOS-2 cells (a–c) microinjected with 10 ng/μl pcDNA3p53wt and 30 ng/μl pcDNA3PML3 were analysed for p53 expression as above and for PML staining using the anti-PML monoclonal antibody PG-M3 followed by incubation with an FITC-conjugated secondary antibody. U2OS cells (d–f) were microinjected with pcDNA3PML3, and localization of endogenous p53 and overexpressed PML3 was examined as above. (C) LOVO cells were treated with UV light and As2O3 and expression of endogenous p53 was detected by a mixture of DO-1 and 1801 monoclonal antibodies followed by incubation with a TRITC-conjugated secondary antibody (a and c). Endogenous PML3 staining was revealed with a rabbit polyclonal serum specific for PML3 and FITC-conjugated secondary antibody (b and c). Download figure Download PowerPoint Since it has been shown that PML plays a crucial role in the assembly of NBs by recruiting other components (Ishov et al., 1999) and that SUMO-1 conjugation of PML is necessary for this process (Zhong et al., 2000a), we next tested whether PML could be involved in the observed relocalization of p53. Immunofluorescence analysis of SaOS-2 cells microinjected with p53 wt together with PML3 (Fagioli et al., 1992) revealed that a fraction of p53 was segregated into PML3-positive NBs in almost all microinjected cells (Figure 1B, a–c). This PML3-dependent recruitment of ectopically expressed p53 into NBs was also observed in MG63, another p53-null cell line, demonstrating that this effect was not cell line specific (not shown). A similar relocalization was obtained for endogenous wt p53 as well, when PML3 expression vector was microinjected in U2OS cells (Figure 1B, d–f). From these observations we can conclude that at least one isoform of PML is required for targeting p53 into NBs. Since overexpression of SUMO-1 and hUbc9 has the same final outcome, it is likely that this enhances the sumolation of endogenous PML and thus augments assembly of NBs and recruitment of p53 into these structures. The presence of PML3 mRNA, as detected by RT–PCR analysis in the employed cell lines (not shown), and the previous evidence that NB recruitment of PML depends on its sumolation (Zhong et al., 2000a), corroborate this interpretation. As2O3 has been shown to increase specifically the modification of PML by SUMO-1 (Muller et al., 1998a), therefore we wanted to test whether, upon treatment with this drug, endogenous p53 could be recruited into PML3-containing NBs. For this aim, LOVO human colon carcinoma cells were treated with As2O3 and prior to fixation were subjected to a hypotonic pre-extraction to remove some of the diffuse nucleoplasmic p53. Although As2O3 treatment increased the number and size of PML3-containing NBs as expected when detected by using a polyclonal serum specific for PML3, endogenous wt p53 was not recruited into these structures (not shown). When cells were irradiated with UV light and treated with As2O3, we clearly detected colocalization between endogenous PML3 and p53 in NBs (Figure 1C). UV treatment alone was not sufficient to change the diffuse nucleoplasmic staining of p53 (not shown). These results therefore demonstrate that colocalization of endogenous p53 and PML3 can be induced under conditions that simultaneously enhance the sumolation of PML and trigger p53 activation. Dissection of the p53 region required for NB localization To identify the region of p53 required for NB targeting, several p53 deletion constructs were generated (Figure 2A) and microinjected into SaOS-2 cells together with PML3. The different p53 proteins were expressed at comparable levels, as judged by western blot analysis after transient transfection (not shown), and showed the typical homogeneous nuclear staining (Figure 2B, a, e, i and m). We found that N-terminal deletions missing the transactivation domain (amino acids 12–69) or the Pro-rich region (amino acids 63–91) of p53 displayed both a nuclear diffuse staining and accumulation in PML3-containing NBs (not shown), as observed for the full-length protein (see Figure 1B). In contrast, p53 294–393, which lacks the N-terminal and core domains did not change its homogeneous nucleoplasmic localization upon coexpression of PML3 (Figure 2B, n–p). These findings suggested that the core domain is required for the relocalization of p53 into NBs. Accordingly, p53 1–298, a protein that contains this domain as well as upstream N-terminal regions, efficiently relocalized into NBs (Figure 2B, j–l). Interestingly, unlike p53 wt and the deletions tested so far, this protein exclusively accumulated in NBs, indicating that sequences in the C-terminus of p53 may possess a negative regulatory role on NB targeting. Deletion of a C-terminal segment until amino acid 363 (Figure 2B, b–d) resulted in a protein that showed a staining pattern similar to the wt protein. On the contrary, p53 1–355, a deletion lacking the last 38 residues of p53, totally relocalized into NBs upon PML3 coexpression (Figure 2B, f–h). Similar results were obtained in MG63 and also when the forementioned p53 deletions were coexpressed with SUMO-1 and hUbc9 (not shown). Figure 2.Direct interaction between PML3 and the core domain of p53 mediates the relocalization of p53 into NBs. (A) Schematic representation of the various p53 deletion mutants and summary of their ability to bind PML3 and to relocalize into NBs. Transactivation (Tr), polyproline (PP), DNA-binding (DBD) and tetramerization and non-specific DNA-binding (Tm/NSDB) domains are indicated. Numbers refer to amino acids. NA, not assessed. (B) SaOS-2 cells were microinjected with 10 ng/μl expression vectors encoding various p53 deletions alone (a, e, i and m) or together with pcDNA3PML3 (30 ng/μl) and analysed for p53 and PML3 staining as described in Figure 1B. (C) SaOS-2 cells were transfected as indicated and immunoprecipitated with an anti-PML antibody (PG-M3). Immunoblotting was performed with the anti-p53 antibody DO-1 (lanes 1–6) or with the anti-HA antibody to detect the HA-tagged p53 294–393 protein (lanes 7 and 8). Lower panels show expression levels of the various overexpressed proteins. Download figure Download PowerPoint These results indicate that the core domain (amino acids 90–298) of p53 is required for NB targeting. However, the evidence that the p53 H175 mutant is also localized to NBs (not shown) demonstrates that the wt conformation of the p53 DNA-binding region is dispensable. Furthermore, the massive PML3-induced relocalization of p53 1–355 as compared with p53 1–363 strongly suggests that eight residues between 356 and 363 exert a regulatory function on this process. p53 binds to PML3 with the domain required for NB targeting Next we investigated whether the PML3-dependent change in p53 subcellular distribution was mediated by direct association between the two proteins. SaOS-2 cells were transfected with plasmids encoding p53 wt or various deletions together with PML3, and cell lysates were immunoprecipitated with the anti-PML antibody PG-M3. The bound protein complexes were analysed by western blotting using the anti-p53 antibody, DO-1, for p53 wt and C-terminal deletion mutants (p53 1–355 and p53 1–298), or anti-HA antibody for the HA-tagged p53 294–393 protein. As shown in Figure 2C, only p53 proteins able to relocalize to NBs were immunoprecipitated from cells expressing PML3. These results clearly indicate that p53 binds to PML3 via its core domain and that this binding mediates p53 targeting to NBs in cells overexpressing PML3. NB targeting of p53 is mediated by a specific PML isoform The integrity of NB structures is disrupted in APL cells, in which expression of the PML–RARα fusion protein leads to the disorganization of PODs into numerous and aberrant microstructures (Hodges et al., 1998). To assess whether p53 recruitment into NBs was specific for PML3 and not for the oncogenic PML–RARα product, SaOS-2 cells were microinjected with plasmids encoding PML–RARα and p53 wt and analysed by immunofluorescence and confocal microscopy. The injected cells showed the typical microspeckled pattern for PML–RARα (Figure 3A, b and c), but the homogeneous nuclear diffuse staining of p53 was not affected (Figure 3A, a and c). Figure 3.Recruitment of p53 to NBs depends on its interaction with a specific PML isoform. (A) SaOS-2 cells microinjected with p53 wt (10 ng/μl) and 30 ng/μl PML–RARα (a–c) or PML-L (d–f) were analysed by immunofluorescence as in Figure 1B. (B) Schematic representation of the various PML proteins showing their functional domains. Upper numbers refer to PML-specific amino acids, lower numbers correspond to residues in RARα. Sumolation sites (S) are indicated. (C) Lysates from SaOS-2 cells transfected with PML3, PML-L or PML–RARα and precipitated with GST–p53 or GST, as indicated, were analysed by western blotting with an anti-PML polyclonal antibody. (D) Pull down experiment performed with in vitro translated p53 wt or different deletions, as indicated, and GST–PML3Ct or GST. Complexes were resolved by SDS–PAGE and visualized by autoradiography. Download figure Download PowerPoint Since the PML–RARα fusion protein is lacking the PML C-terminal region (Figure 3B), we hypothesized that this domain is required to target p53 into NBs. PML-L, another PML splice variant, which differs only in its short C-terminal tail from the PML3 protein employed so far (Figure 3B), was coinjected with p53 wt into SaOS-2 cells and the immunostaining pattern was analysed as above. Although PML-L, as expected, formed NBs where other resident proteins, like Sp100 and SUMO-1, were found to localize (not shown), the distribution of p53 remained diffuse in the injected cells (Figure 3A, d–f). Parallel experiments performed with p53 1–355 gave similar results (not shown). In vitro binding experiments with glutathione S-transferase (GST)–p53 on lysates from cells expressing PML3, PML-L or PML–RARα demonstrated that p53 wt binds efficiently only to PML3, while the interaction with PML-L and with PML–RARα was severely impaired (Figure 3C). A GST fusion containing the 61-residue-long C-terminal tail specific for PML3 (Figure 3B), efficiently bound to in vitro translated p53 wt, p53 1–355 and p53 1–298 (Figure 3D). These results indicate that a region outside the central domain of PML, where the three known sumolation sites have been mapped (Kamitani et al., 1998) and that is present in the PML3 isoform, is necessary and sufficient to mediate p53 binding. PML3 affects cell survival in a p53-dependent way Since NBs and PML have been linked to regulation of cell growth and differentiation (Lin et al., 1999) and p53 is a well established tumour suppressor (Sionov and Haupt, 1999), we examined whether recruitment of p53 into NBs can modulate cell survival. U2OS and MG63 cells were microinjected with plasmids encoding PML3 or human placental alkaline phosphatase (PLAP) as a negative control together with a GFP expression vector as marker. Twenty-four hours later cell survival was scored as the number of recovered cells positive for the intrinsic green fluorescence of GFP. U2OS cells express wt p53 that, upon introduction of PML3, was efficiently recruited into NBs (Figure 1B, d–f), while MG63 cells lack endogenous p53. Upon overexpression of PML3, we consistently observed a significant reduction of survival in U2OS (Figure 4A, left panel) but not in MG63 cells (Figure 4A, right panel). To correlate the observed phenotype with PML3-mediated recruitment of p53 into NBs, we analysed whether overexpression of p53 1–355 (the C-terminal deletion that totally relocalized to NBs upon PML3 expression, see Figure 2B) could restore the PML3-dependent effect on MG63 cells. Cell survival was analysed by microinjecting MG63 cells either with PML3 and p53 1–355 alone or with a combination of the two plasmids, and was scored as above. As plotted in Figure 4B, recovery of GFP-positive cells was severely impaired when both PML3 and p53 1–355 were simultaneously expressed (bar 4), while no effect was observed when the two proteins were individually expressed (bars 2 and 3). Under the same conditions, PML-L, the isoform impaired in binding and relocalizing p53 into NBs, did not affect cell survival when coexpressed with p53 1–355 (Figure 4B, bar 6). Figure 4.PML3 affects cell survival in a p53-dependent way. (A) U2OS (left panel) and MG63 (right panel) cells were microinjected with the indicated plasmids (30 ng/μl) together with pGFP (15 ng/μl) as marker. Twenty-four hours later cell survival was scored as the percentage of recovered cells positive for GFP. (B) Cell survival assay was performed in MG63 cells after ectopic expression of p53 1–355 (20 ng/μl), PML3 (30 ng/μl) and PML-L (30 ng/μl), either alone (bars 2, 3 and 5) or in combinations (bars 4 and 6). PLAP was used as negative control and to adjust total DNA amount in all the samples. Graphs represent the mean of at least seven independent experiments. Download figure Download PowerPoint These results therefore suggest that recruitment of p53 into NBs by a specific PML isoform modulates the survival functions linked to these structures. Binding of p53 to a specific PML isoform, PML3, increases its transcriptional activity The evidence that PML associates with several transcription factors and coactivators, like p300/CBP and recruits them into NBs, suggests a relevant role for PML and the whole NB structure in transcriptional control (Zhong et al., 2000b). We therefore analysed whether the observed effect on cell survival upon ectopic expression of PML3 was linked to changes in p53 transactivation ability. Transient transfection assays with constructs containing two well established p53 responsive promoters, PIG3 (Polyak et al., 1997) and p21 (El-Deiry et al., 1993), cloned upstream of the luciferase reporter were performed in MG63 cells with combinations of p53 wt, p53 1–355, PML3 and PML-L expression vectors. Coexpression of p53 wt with PML3 strongly increased the transcriptional activity of p53 toward the PIG3 promoter (Figure 5A, bars 6 and 8), while under the same conditions PML-L overexpression showed no significant effect (Figure 5A, bars 6 and 10). Consistent with previous reports (Tarunina and Jenkins, 1993), p53 1–355 alone transactivated the p53-responsive promoters, although to a lesser extent as compared with p53 wt (Figure 5A and B, bars 5 and 6). However, upon coexpression of PML3, the transcriptional activity of p53 1–355 was increased to values comparable to the ones obtained with p53 wt under the same conditions (Figure 5B, bar 8). This was directly dependent on the ability of p53 1–355 to bind to PML3, since a significantly reduced effect was observed upon coexpression of p53 1–355 and PML-L (Figure 5B, bar 10). Interestingly, the PML3-mediated enhancement of p53 transactivation ability was much less evident when tested on the p21 promoter. As shown in Figure 5A and B, only a slight increase in p21 luciferase activity was detected when PML3 was coexpressed with either p53 wt or p53 1–355 (compare bars 5 and 7). The differential promoter transactivations were not due to differences in the level of expression of the various p53 proteins, as judged by western blot analysis (Figure 5, lower panels). Figure 5.PML3 enhances p53 transcriptional activity in a promoter-specific manner. Transactivation of p53 wt (A) and p53 1–355 (B) toward the p21-LUC or the PIG3-LUC reporters was assayed by transfecting MG63 cells with pcDNA3p53wt or pcDNA3p53 1–355, either alone or together with PML3 or PML-L, as indicated. Renilla luciferase reporter (pRL-CMV; 50 ng) was cotransfected in each case to normalize transfection efficiency. Graphs represent the mean of at least four independent experiments. An aliquot of each lysate was analysed by western blotting with DO-1 antibody to demonstrate comparable levels of expression of p53 in all the samples (lower panels). Download figure Download PowerPoint These findings therefore demonstrate that PML3 is able to enhance p53-dependent transactivation in a promoter-specific manner. Of note, PIG3 belongs to a group of p53-regulated genes with the potential to induce oxidative stress and apoptosis (Polyak et al., 1997), thus providing a possible link between the observed decrease in cell survival and the specific activation of the PIG3 promoter foll