Title: IMMUNOCYTOCHEMICAL CHARACTERIZATION OF THE INTRAPYRENOID THYLAKOIDS OF CRYPTOMONADS<sup>1</sup>
Abstract: ABSTRACT Thylakoid lamellae extend into the pyrenoids of only two genera of cryptomonad algae, Chroomonas and Hemiselmis , We used immunoelectron microscopy to assess the photosynthetic competency of cryptomonad intrapyrenoid thylakoids. Intrapyrenoid thylakoids possess phycobiliproteins and the chlorophyll a/c 2 light‐harvesting complex, both of which are associated with photosystem (PS) II in a light‐harvesting capacity. In addition, thylakoids that extend into the pyrenoid of Hemiselmis brunnescens were immunolabelled by anti‐PSI. These results indicate that cryptomonad intrapyrenoid thylakoids likely function in a manner analogous to thylakoids of the chloroplast stroma. Moreover, our observation that the Calvin cycle enzyme ribulose 1,5‐bisphosphate carboxylase/oxygenase (Rubisco) is pyrenoid‐localized in these two cryptophytes indicates that the processes of photosynthetic O 2 ‐evolution and ribulose 1,5‐bisphosphate (RuBP) carboxylation/oxygenation are not spatially separated in these algae.
Publication Year: 1992
Publication Date: 1992-02-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 13
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