Title: Real‐time PCR and subsequent pyrosequencing for screening of <i>penA </i>mosaic alleles and prediction of reduced susceptibility to expanded‐spectrum cephalosporins in <i>Neisseria gonorrhoeae</i>
Abstract: APMISVolume 116, Issue 11 p. 1004-1008 Real-time PCR and subsequent pyrosequencing for screening of penA mosaic alleles and prediction of reduced susceptibility to expanded-spectrum cephalosporins in Neisseria gonorrhoeae Magnus Unemo, Corresponding Author Magnus UnemoMagnus Unemo, National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Örebro University Hospital, SE-701 85 Örebro, Sweden. e-mail: [email protected]Search for more papers by this authorPer Olcén, Per Olcén National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Örebro University Hospital, Örebro, SwedenSearch for more papers by this authorHans Fredlund, Hans Fredlund National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Örebro University Hospital, Örebro, SwedenSearch for more papers by this authorSara Thulin, Sara Thulin National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Örebro University Hospital, Örebro, SwedenSearch for more papers by this author Magnus Unemo, Corresponding Author Magnus UnemoMagnus Unemo, National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Örebro University Hospital, SE-701 85 Örebro, Sweden. e-mail: [email protected]Search for more papers by this authorPer Olcén, Per Olcén National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Örebro University Hospital, Örebro, SwedenSearch for more papers by this authorHans Fredlund, Hans Fredlund National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Örebro University Hospital, Örebro, SwedenSearch for more papers by this authorSara Thulin, Sara Thulin National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Örebro University Hospital, Örebro, SwedenSearch for more papers by this author First published: 30 December 2008 https://doi.org/10.1111/j.1600-0463.2008.01062.xCitations: 11 † Received 20 January 2008. Accepted 2 April 2008. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat REFERENCES 1 CDC. Sexually Transmitted Disease Surveillance 2004 Supplement: Gonococcal Isolate Surveillance Project (GISP) Annual Report – 2004. Atlanta Georgia : U.S. Department of Health and Human Services, 2005. 2 Deguchi T, Yasuda M, Yokoi S, Ishida K, Ito M, Ishihara S, et al. Treatment of uncomplicated gonococcal urethritis by double-dosing of 200 mg cefixime at a 6-h interval. J Infect Chemother 2003; 9: 35–9. 3 Dillon JA, Ruben M, Li H, Borthagaray G, Marquez C, Fiorito S, et al. Challenges in the control of gonorrhoea in South America and the Caribbean: monitoring the development of resistance to antibiotics. Sex Transm Dis 2006; 33: 87–95. 4 Martin IM, Hoffmann S, Ison CA. European Surveillance of Sexually Transmitted Infections (ESSTI). The first combined antimicrobial susceptibility data for Neisseria gonorrhoeae in Western Europe. J Antimicrob Chemother 2006; 58: 587–93. 5 The WHO Western Pacific Gonococcal Antimicrobial Surveillance Programme. Surveillance of antibiotic resistance in Neisseria gonorrhoeae in the WHO Western Pacific Region, 2004. Commun Dis Intell 2006; 30: 129–32. 6 Ameyama S, Onodera S, Takahata M, Minami S, Maki N, Endo K, et al. Mosaic-like structure of penicillin-binding protein 2 gene (penA) in clinical isolates of Neisseria gonorrhoeae with reduced susceptibility to cefixime. Antimicrob Agents Chemother 2002; 46: 3744–9. 7 Ito M, Deguchi T, Mizutani KS, Yasuda M, Yokoi S, Ito S, et al. Emergence and spread of Neisseria gonorrhoeae clinical isolates harboring mosaic-like structure of penicillin-binding protein 2 in Central Japan. Antimicrob Agents Chemother 2005; 49: 137–43. 8 Lindberg R, Fredlund H, Nicholas R, Unemo M. Neisseria gonorrhoeae isolates with reduced susceptibility to cefixime and ceftriaxone: association with genetic polymorphisms in penA, mtrR, porB1b, and ponA. Antimicrob Agents Chemother 2007; 51: 2117–22. 9 Takahata S, Senju N, Osaki Y, Yoshida T, Ida T. Amino acid substitutions of mosaic penicillin-binding protein 2 associated with reduced susceptibility to cefixime in clinical isolates of Neisseria gonorrhoeae. Antimicrob Agents Chemother 2006; 50: 3638–45. 10 Tanaka M, Nakayama H, Huruya K, Konomi I, Irie S, Kanayama A, et al. Analysis of mutations within multiple genes associated with resistance in a clinical isolate of Neisseria gonorrhoeae with reduced ceftriaxone susceptibility that shows a multidrug-resistant phenotype. Int J Antimicrob Agents 2006; 27: 20–6. 11 Wang SA, Lee MV, O'Connor N, Iverson CJ, Ohye RG, Whiticar PM, et al. Multidrug-resistant Neisseria gonorrhoeae with decreased susceptibility to cefixime—Hawaii, 2001. Clin Infect Dis 2003; 37: 849–52. 12 Dowson CG, Jephcott AE, Gough KR, Spratt BG. Penicillin-binding protein 2 genes of non-beta-lactamase-producing, penicillin-resistant strains of Neisseria gonorrhoeae. Mol Microbiol 1989; 3: 35–41. 13 Ochiai S, Sekiguchi S, Hayashi A, Shimadzu M, Ishiko H, Matsushima-Nishiwaki R, et al. Decreased affinity of mosaic-structure recombinant penicillin-binding protein 2 for oral cephalosporins in Neisseria gonorrhoeae. J Antimicrob Chemother 2007; 60: 54–60. 14 Whiley DM, Limnios EA, Ray S, Sloots TP, Tapsall JW. Further questions regarding the role of mosaic penA sequences in conferring reduced susceptibility to ceftriaxone in Neisseria gonorrhoeae. Antimicrob Agents Chemother 2007; 51: 802–3. 15 Whiley DM, Limnios EA, Ray S, Sloots TP, Tapsall JW. Diversity of penA alterations and subtypes in Neisseria gonorrhoeae strains from Sydney, Australia, that are less susceptible to ceftriaxone. Antimicrob Agents Chemother 2007; 51: 3111–6. 16 Whiley D, Bates J, Limnios A, Nissen MD, Tapsall J, Sloots TP. Use of a novel screening PCR indicates presence of Neisseria gonorrhoeae isolates with a mosaic penA gene sequence in Australia. Pathology 2007; 39: 445–6. 17 Mavroidi A, Tzouvelekis LS, Kyriakis KP, Avgerinou H, Daniilidou M, Tzelepi E. Multidrug-resistant strains of Neisseria gonorrhoeae in Greece. Antimicrob Agents Chemother 2001; 45: 2651–4. 18 Thulin S, Olcén P, Fredlund H, Unemo M. A combined real-time PCR and pyrosequencing strategy for objective, sensitive, specific, and high throughput identification of reduced susceptibility to penicillins in Neisseria meningitidis. Antimicrob Agents Chemother 2007; 52: 753–6. Citing Literature Volume116, Issue11November 2008Pages 1004-1008 ReferencesRelatedInformation
Publication Year: 2008
Publication Date: 2008-11-01
Language: en
Type: letter
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 15
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