Title: Identification of sweet cherry (Prunus avium L.) cultivars using AFLP and SSR markers.
Abstract:The genetic characterization of 19 Lithuania-bred and 5 common sweet cherry (Prunus avium L.) cultivars using microsatellite (SSR) and amplified fragment length polymorphism (AFLP) markers is presente...The genetic characterization of 19 Lithuania-bred and 5 common sweet cherry (Prunus avium L.) cultivars using microsatellite (SSR) and amplified fragment length polymorphism (AFLP) markers is presented. The genetic diversity of sweet cherry cultivars was evaluated using 13 previously published SSR primer pairs and 9 AFLP primer combinations. Based on SSR, AFLP and combined data, three dendrograms were created. Comparison of both marker systems showed that the probability of correct clustering of cultivars in the dendrogram is higher for AFLP markers than for SSR markers. The highest probability of correct clustering of cultivars in the dendrogram was obtained when combined data on fragments obtained using both marker systems was used and this data gives a more comprehensive description of the genome studied. The SSR primer pairs PCEGA34 and EMPAS06 enable identification of the investigated sweet cherry cultivars group. All studied cultivars were identified using polymorphic fragments amplified with AFLP EcoRI-AC/MseI-CG primer combination with the highest resolving power value. It was established that the 23 AFLP fragments, generated with the EcoRI-AC/MseI-CG primer combination and exhibiting the highest polymorphism information content (PIC) values (from 0.28 to 0.32), were sufficient for sweet cherry cultivar identification. Fragments profile and their number required for the identification of an individual cultivar were different. Molecular profiles with minimal number of SSR and AFLP markers necessary for identification of studied sweet cherry cultivars are presented.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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Cited By Count: 14
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