Title: Cytoplasmic domain of δ subunit is important for the extra-synaptic targeting of GABA<sub>A</sub>receptor subtypes
Abstract: Journal of Integrative NeuroscienceVol. 13, No. 04, pp. 617-631 (2014) ArticlesNo AccessCytoplasmic domain of δ subunit is important for the extra-synaptic targeting of GABAA receptor subtypesAyla Arslan, Jakob von Engelhardt, and William WisdenAyla ArslanDepartment of Clinical Neurobiology, University of Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, GermanyDepartment of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Üsküdar University, Altunizade Mah. Haluk Türksoy Sok. No: 14, PK 34662 Üsküdar/İstanbul, TurkeyCorresponding author., Jakob von EngelhardtDepartment of Clinical Neurobiology, University of Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, GermanyGerman Center for Neurodegenerative Diseases (DZNE), c/o DKFZ / A300 Im Neuenheimer Feld 280, 69120 Heidelberg, Germany, and William WisdenDepartment of Clinical Neurobiology, University of Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, GermanyDepartment of Life Sciences, Imperial College London, South Kensington, London SW7 2AZ, UKhttps://doi.org/10.1142/S0219635214500228Cited by:3 PreviousNext AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsRecommend to Library ShareShare onFacebookTwitterLinked InRedditEmail AbstractGABAA receptors (GABAARs) are hetero-pentameric chloride channels and the primary sites for fast synaptic inhibition. We have expressed recombinant γ2 and δ subunits of GABAARs in cultured hippocampal neurons to analyze the membrane targeting of synaptic and extra-synaptic GABAARs, a phenomenon not well understood. Our data demonstrate that the synaptic targeting of γ2-containing GABAARs (γ2-GABAARs) does not depend on the cytoplasmic loop of γ2 subunit, in parallel with previous findings, showing that the synaptic localization of γ2-GABAARs requires the TM4 domain of γ2 rather than the large cytoplasmic loop. On the other hand, we showed here that the extrasynaptic targeting of the δ-containing GABAARs (δ-GABAARs) depends on the cytoplasmic loop of δ subunit via an active or a passive mechanism. We also show that the amino acid sequences of δ loop is highly conserved across the whole span of vertebrate evolution suggesting an active role of δ loop in extra-synaptic targeting of corresponding receptor subtypes.Keywords:GABAA receptorsgamma-2 subunitdelta subunitsynapticextra-synaptic References M. J. 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Publication Year: 2014
Publication Date: 2014-12-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 6
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