Title: Cholesterol and lipid microdomains stabilize the postsynapse at the neuromuscular junction
Abstract: Article24 August 2006free access Cholesterol and lipid microdomains stabilize the postsynapse at the neuromuscular junction Raffaella Willmann Raffaella Willmann Department of Neurochemistry, Brain Research Institute, University of Zürich, Zürich, Switzerland Search for more papers by this author San Pun San Pun Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland Search for more papers by this author Lena Stallmach Lena Stallmach Department of Neurochemistry, Brain Research Institute, University of Zürich, Zürich, Switzerland Search for more papers by this author Gayathri Sadasivam Gayathri Sadasivam Department of Neurochemistry, Brain Research Institute, University of Zürich, Zürich, Switzerland Search for more papers by this author Alexandre Ferrao Santos Alexandre Ferrao Santos Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland Search for more papers by this author Pico Caroni Pico Caroni Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland Search for more papers by this author Christian Fuhrer Corresponding Author Christian Fuhrer Department of Neurochemistry, Brain Research Institute, University of Zürich, Zürich, Switzerland Search for more papers by this author Raffaella Willmann Raffaella Willmann Department of Neurochemistry, Brain Research Institute, University of Zürich, Zürich, Switzerland Search for more papers by this author San Pun San Pun Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland Search for more papers by this author Lena Stallmach Lena Stallmach Department of Neurochemistry, Brain Research Institute, University of Zürich, Zürich, Switzerland Search for more papers by this author Gayathri Sadasivam Gayathri Sadasivam Department of Neurochemistry, Brain Research Institute, University of Zürich, Zürich, Switzerland Search for more papers by this author Alexandre Ferrao Santos Alexandre Ferrao Santos Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland Search for more papers by this author Pico Caroni Pico Caroni Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland Search for more papers by this author Christian Fuhrer Corresponding Author Christian Fuhrer Department of Neurochemistry, Brain Research Institute, University of Zürich, Zürich, Switzerland Search for more papers by this author Author Information Raffaella Willmann1, San Pun2, Lena Stallmach1, Gayathri Sadasivam1, Alexandre Ferrao Santos2, Pico Caroni2 and Christian Fuhrer 1 1Department of Neurochemistry, Brain Research Institute, University of Zürich, Zürich, Switzerland 2Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland *Corresponding author. Department of Neurochemistry, Brain Research Institute, University of Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland. Tel.: +41 44 635 33 10; Fax: +41 44 635 33 03; E-mail: [email protected] The EMBO Journal (2006)25:4050-4060https://doi.org/10.1038/sj.emboj.7601288 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Stabilization and maturation of synapses are important for development and function of the nervous system. Previous studies have implicated cholesterol-rich lipid microdomains in synapse stabilization, but the underlying mechanisms remain unclear. We found that cholesterol stabilizes clusters of synaptic acetylcholine receptors (AChRs) in denervated muscle in vivo and in nerve–muscle explants. In paralyzed muscles, cholesterol triggered maturation of nerve sprout-induced AChR clusters into pretzel shape. Cholesterol treatment also rescued a specific defect in AChR cluster stability in cultured src−/−;fyn−/− myotubes. Postsynaptic proteins including AChRs, rapsyn, MuSK and Src-family kinases were strongly enriched in lipid microdomains prepared from wild-type myotubes. Microdomain disruption by cholesterol-sequestering methyl-β-cyclodextrin disassembled AChR clusters and decreased AChR–rapsyn interaction and AChR phosphorylation. Amounts of microdomains and enrichment of postsynaptic proteins into microdomains were decreased in src−/−;fyn−/− myotubes but rescued by cholesterol treatment. These data provide evidence that cholesterol-rich lipid microdomains and SFKs act in a dual mechanism in stabilizing the postsynapse: SFKs enhance microdomain-association of postsynaptic components, whereas microdomains provide the environment for SFKs to maintain interactions and phosphorylation of these components. Introduction Synaptogenesis is a key process in the development and function of the nervous system. In a first phase, neurotransmitter receptors and associated proteins accumulate to form a postsynaptic density. Proteins and nonprotein factors trigger synaptic differentiation (Fox and Umemori, 2006). A major factor is glia-derived cholesterol, which induces synaptogenesis in cultured retinal ganglion cells (Mauch et al, 2001). Its roles in vivo and specifically in postsynaptic assembly remain unknown. In a second phase of synaptogenesis, some synapses and postsynaptic densities mature and are stabilized, while others are eliminated. Whereas neural activity is known to regulate this process (Cohen-Cory, 2002), the effector machinery in synapse stabilization is poorly understood. Cholesterol, along with sphingolipids, is enriched in subcompartments of the cellular membrane system. These lipid microdomains, often isolated due to their detergent-resistance, also include signaling proteins, regulate trafficking and signal transduction processes, and may partly correspond to membrane rafts, dynamic structures that bring together activated receptors and transducer molecules (Brown and London, 1998; Simons and Toomre, 2000; Golub et al, 2004; Pike, 2006). The cholesterol-rich lipid microdomains (CRLMs) are involved in aspects of synaptic function in cultured cells. Depletion of cholesterol leads to loss of surface AMPA receptors and of synapses in hippocampal neurons (Hering et al, 2003). In ciliary neurons, CRLMs are necessary for the maintenance of α7 neuronal nicotinic acetylcholine receptors (AChRs) in synapse-associated clusters (Bruses et al, 2001). At the neuromuscular junction (NMJ), the presence of plasmalemmal cholesterol is necessary for proper AChR gating functions (Barrantes, 1993); AChRs associate with CRLMs in trafficking toward the plasma membrane in transfected heterologous cells (Marchand et al, 2002). However, the relevance of lipid microdomains and cholesterol for synaptogenesis in vivo, and the identity of the signaling pathways operating through the microdomains, has remained unclear. During NMJ formation, myotubes respond to neural agrin, assembling AChRs at nascent synapses (Gautam et al, 1996). This scaffolding function is assigned to MuSK, the trans-membrane kinase that translates agrin into a clustering signal (Glass et al, 1996). Besides MuSK and AChR, rapsyn is the third core protein for the AChR clustering process (Gautam et al, 1995; Marangi et al, 2001). In response to agrin, the association of rapsyn with AChRs increases and mediates binding to cytoskeletal proteins (Moransard et al, 2003). AChR β subunits become tyrosine-phosphorylated and this modification regulates cytoskeletal linkage and efficient clustering (Borges and Ferns, 2001). During the maturation of NMJs, plaque-shaped AChR clusters are stabilized and adopt pretzel-shaped configurations, with AChRs located at the crests of postjunctional folds. AChR half-life time is highly increased and synaptic proteins are selectively produced by subsynaptic nuclei (Sanes and Lichtman, 2001). The molecular mechanisms mediating this postnatal NMJ stabilization differ from those involved in NMJ induction, and much less is known about them (Willmann and Fuhrer, 2002). Stability of AChR clusters can also be analyzed in cultured myotubes, by adding and then withdrawing agrin or other factors and studying the half-life time of clusters. Although the time scale is different, this assay reveals many parallels to postnatal NMJ stabilization in vivo. Thus, the utrophin complex with its components dystroglycan and dystrobrevin, and Src-family kinases (SFKs) are important in postsynaptic stabilization both in vivo and in vitro (Grady et al, 2000; Jacobson et al, 2001; Smith et al, 2001; Marangi et al, 2002; Sadasivam et al, 2005). SFKs are activated by agrin (Mittaud et al, 2001) and maintain AChR–rapsyn interaction and AChR β phosphorylation (Sadasivam et al, 2005). In cultured src−/−;fyn−/− myotubes, agrin- or laminin-induced AChR clusters are unstable and disperse rapidly after withdrawal of these factors (Smith et al, 2001; Marangi et al, 2002). Interfering with SFK function in vivo causes postsynaptic disintegration of adult NMJs (Sadasivam et al, 2005). Since SFK functions are specifically associated with CRLMs in other cells (Resh, 1999; Simons and Toomre, 2000), these results raise the possibility that microdomain-dependent processes might be involved in postsynaptic apparatus maintenance through SFKs. To investigate mechanisms of postsynaptic maturation, we determined whether, and through what signaling molecules, cholesterol and lipid microdomains might stabilize NMJs in vivo and in vitro. We find that cholesterol addition stabilizes NMJs and promotes their maturation from patch- to pretzel-type configurations. Postsynaptic proteins reside in CRLMs, and CRLM dispersion disrupts AChR clusters, AChR–rapsyn interaction and AChR β phosphorylation. In src−/−;fyn−/− myotubes, cholesterol addition normalizes the reduced CRLM association of postsynaptic proteins and stabilizes AChR clusters. These results suggest a dual mechanism for postsynaptic cluster stabilization through SFKs, involving an enhancement of the association of cluster components with CRLMs, and interactions and phosphorylation of these components at the microdomains. Results Cholesterol stabilizes AChR clusters in vivo To investigate a possible role of cholesterol and lipid microdomains in promoting postsynaptic apparatus maintenance in vivo, we analyzed the state of assembly of AChR clusters at denervated NMJs in the absence or presence of exogenous cholesterol. The sciatic nerve was cut in 1-month-old mice, and AChR clusters were visualized 12 days later in two DeSyn muscles (lateral gastrocnemius and medial gastrocnemius), which exhibit substantial postsynaptic cluster disassembly under these experimental conditions (Pun et al, 2002). To visualize denervated synaptic sites, we counterstained muscle sections with an antibody against p75, a protein upregulated in Schwann cells in the absence of nerve contact (Taniuchi et al, 1986). As expected, denervated synaptic sites exhibited only remnants of AChR clusters after 12 days of denervation (Figure 1A; note irregular AChR labeling patterns, with only small regions of intense labeling). In contrast, when cholesterol was applied daily to denervated muscles, starting 5 days after denervation, AChR signals at denervated synaptic sites were much better preserved (Figure 1A; note that p75 signals were not affected by the cholesterol treatment). These AChR signals were comparable to clusters in nondenervated control animals (not shown, but refer to an earlier paper (Pun et al, 2002)). A quantitative analysis of AChR labeling intensities revealed that synaptic sites had lost most of their AChR signal 12 days after denervation, but that the synaptic signal was largely preserved in the presence of exogenous cholesterol (Figure 1C). Figure 1.Cholesterol stabilizes AChR clusters in denervated muscles. (A) Appearance of AChR clusters in two DeSyn muscles 12 days after denervation. Sciatic nerves were cut in 1 month mice; the absence of intact axons is confirmed by the expression of p75 in Schwann cells. Where indicated, cholesterol was applied daily, starting 5 days after denervation. The larger p75-positive area in the medial gastrocnemius panel with cholesterol is due to the particular plane of section, and does not reflect a systematic elevation of Schwann cell p75 immunofluorescence signals in denervated muscles treated with cholesterol. (B) Examples of AChR clusters (visualized by rhodamine-α-bungarotoxin; RITC-α-BT) in soleus nerve–muscle explants after 3 h in vitro. (C) Quantitative analysis of data as shown in (A) (left) and (B) (right). AChR labeling intensities (RITC-α-BT) were compared to controls; shown are fractions of NMJs with signal at least 70%, or less than 20% of control values. The 20 and 70% boundaries were selected to highlight the differences among the samples of these experiments. N=300 AChR clusters (from 3 mice each). Bars: 40 (A) and 20 μm (B). Download figure Download PowerPoint To investigate AChR cluster protection by cholesterol under more challenging experimental conditions, we analyzed nerve–muscle explant preparations of soleus maintained at 37°C in Ringer solution supplemented with calcium. To reliably identify synaptic sites, we carried out these experiments using transgenic mice expressing a synaptophysin-GFP construct in neurons (Thy1-spGFPmu) (De Paola et al, 2003). Under these experimental conditions, many synaptic sites lost most of their AChR signal after 3 h ex vivo, such that about half the synapses appeared normal while others had only low-intensity AChR label (Figure 1B and C). Inclusion of the cholesterol sequestering agent methyl-β-cyclodextrin, which disrupts CRLMs (Simons and Toomre, 2000; Tansey et al, 2000; Ma et al, 2003), accelerated the loss of AChR signal (Figure 1B and C). In contrast, inclusion of cholesterol in the culture medium protected most AChR clusters (Figure 1B and C). To determine whether cholesterol might also promote the assembly of new AChR clusters in vivo, we used reporter mice expressing membrane-targeted GFP in neurons (Thy1-mGFPs) (De Paola et al, 2003) and carried out cholesterol supplementation experiments in lateral gastrocnemius muscle chronically treated with Botulinum toxin A. These experimental conditions (lateral gastrocnemius in 1-month-old mice; toxin applications every 4th day for a total of 20 days) induce the disassembly of postsynaptic apparatus at NMJs, a massive nerve sprouting response, and induction of small ectopic AChR plaques along the nerve sprouts (Figure 2A and B (left panels); see also Santos and Caroni, 2003). Daily local applications of cholesterol from day 10 of the BotA treatment, that is, at a time when NMJ disassembly and nerve sprouting were not yet pronounced (Santos and Caroni, 2003), led to a suppression of the AChR cluster disassembly process, which was accompanied by a suppression of nerve sprouting and of ectopic AChR plaque induction by sprouts in these paralyzed muscles (Figure 2A, right panels). The resulting AChR signals appeared very similar to those in nontreated control animals (not shown; but refer to Santos and Caroni, 2003). Significantly, initiation of the cholesterol treatment at day 15, when sprouting was well advanced (Santos and Caroni, 2003), led to the assembly of large, pretzel-shaped ectopic AChR clusters along the sprouts (Figure 2B). Figure 2.Cholesterol promotes AChR cluster assembly at original and ectopic NMJs in paralyzed DeSyn muscles. Low- (A) and high-magnification (B) views of presynaptic nerves (mGFP) and postsynaptic AChR clusters (RITC-α-BT) in lateral gastrocnemius muscles treated with Botulinum toxin A (BotA). The chronic BotA treatment elicited a massive nerve sprouting response in this DeSyn muscle; cholesterol promoted AChR cluster assembly, and inhibited nerve sprouting. Note pretzel-shaped AChR clusters (arrows, right) induced by sprouts (arrows, left) in the presence of exogenous cholesterol. Bars: 200 (A) and 40 μm (B). Download figure Download PowerPoint Taken together, these data provide evidence that local applications of exogenous cholesterol in vivo protect AChR clusters against denervation-induced disassembly, and promote the maturation of sprout-induced ectopic AChR clusters in paralyzed muscles from an embryonic-type plaque shape into a pretzel shape. We thus propose that cholesterol is an important factor for the maturation and stabilization of the NMJ in vivo. Cholesterol stabilizes AChR clusters in cultured src−/−;fyn−/− myotubes To analyze the mechanism of action of cholesterol in stabilizing AChR clusters, we turned to aggregation assays in cultured myotubes. Furthermore, we took advantage of cells from mice lacking Src and Fyn, where AChR clusters are normally induced by agrin or laminin treatment, but disassemble within a few hours after the removal of these factors from the medium (Smith et al, 2001; Marangi et al, 2002). We treated src−/−;fyn−/− myotubes with agrin to induce maximal AChR clustering, then withdrew agrin and determined whether the addition of cholesterol might stabilize AChR clusters. We found that after 5 h, cholesterol-treated cells showed the same number of AChR clusters as cells from which agrin was not withdrawn (Figure 3). Cells from which agrin was withdrawn for 5 h, without addition of cholesterol, showed a low cluster number, comparable to the level of spontaneous clustering. In wild-type cells, clusters were very stable following removal of agrin, as published previously (Smith et al, 2001; Marangi et al, 2002). This stability prohibited assessing significant effects of cholesterol. Figure 3.Cholesterol stabilizes AChR clusters in src−/−;fyn−/− myotubes. (A) src−/−;fyn−/− myotubes were not treated or stimulated overnight with 1 nM agrin to induce AChR clusters (top row). Agrin was withdrawn, cells were washed and incubated for 5 h in agrin-free medium lacking (bottom left) or containing (bottom right) 75 μM cholesterol. Myotubes were stained with rhodamine-α-BT to visualize AChR clusters. (B) For cluster quantification, visual fields covering about three times the area of a panel shown in (A) were taken, and only compact clusters with a mimimum size of 5 μm were counted. Download figure Download PowerPoint We next determined whether cholesterol might compensate for agrin withdrawal by enhancing signaling processes involved in the formation of the NMJ. Cholesterol addition to wild-type myotubes did not induce formation of AChR clusters (Figure 4A) and did not lead to phosphorylation of MuSK or the β-subunit of AChRs, unlike agrin (Figure 4B and C). These data show that cholesterol stabilizes AChR clusters in cultured myotubes but does not activate agrin/MuSK signaling. Figure 4.Cholesterol does not induce AChR clustering and phosphorylation of MuSK and AChR β subunits. (A) Cholesterol or 1 nM agrin were added overnight to C2C12 myotubes. Cells were stained with rhodamine-α-BT and AChR clusters quantitated as in Figure 3. (B, C) C2C12 myotubes were treated with different doses of cholesterol, or with 1 nM agrin for 40 min, as indicated; C, untreated control. From cell lysates, MuSK was immunoprecipitated (B) or AChRs were precipitated using biotin-α-BT and streptavidin-agarose (Tox-P; C). Phosphotyrosine immunoblotting detected phosphorylation of MuSK and AChR β subunits. The identity of these phosphoproteins was confirmed by reprobing with MuSK- or AChR β-specific antibodies (not shown). Download figure Download PowerPoint The proteins involved in AChR cluster stabilization reside in CRLMs Cholesterol is a key component of lipid microdomains, and its action in cluster stabilization might involve microdomain-dependent processes. We therefore prepared and analyzed CRLMs from cultured wild-type myotubes using a well-established detergent-free protocol (Song et al, 1996a, 1996b; Riddell et al, 2001; Nishio et al, 2004; Rhainds et al, 2004; Zhang et al, 2005). Cell homogenates were floated on discontinuous sucrose gradients, and fractions were analyzed by immunoblots. The CRLM fractions (4–6) were found at the interface between 5 and 35% sucrose and defined by strong enrichment of typical markers such as caveolin-3, flotillin-2, cholesterol and the sphingolipid, ganglioside GM1 (Figure 5A and B). Measurement of protein concentration showed that fractions 4–6 contained little of the overall protein (only 9.5±0.2%; mean±s.e.m., n=5); most protein was found at the bottom of the gradient, in fractions 8–12 which contain free (not microdomain associated) cellular proteins (Figure 5C). Another negative control was α-tubulin, which did not associate with CRLMs and was mostly recovered in the free fractions (Figure 5A). These controls established the validity of the method for preparation of CRLMs from myotubes. Figure 5.Postsynaptic proteins associate with CRLMs in myotubes. CRLMs were prepared from wild-type myotubes (C2C12 or clones SW10 and SW5), and fractions of the discontinuous sucrose gradients were collected. Fractions 9–12 represent the bottom gradient step (45% sucrose) containing the total cell extract. Fractions 5–8 represent the 35% sucrose layer and fractions 1–4 the top layer (5% sucrose). (A) Fractions were analyzed by immunoblotting (α-tubulin, caveolin-3, flotillin-2) or dot blotting (ganglioside GM1). Fractions 4–6 contain CRLMs and α-tubulin served as a negative control. (B) Fractions were analyzed for the content of cholesterol, showing high enrichment in CRLM fractions 4–6 (n=4). (C) Protein assays of gradient fractions reveal the bulk of protein in the bottom fractions, illustrating the specificity of the CRLM preparation (n=4). (D) Fractions were subjected to immunoblotting, showing that MuSK, AChRs (β-subunit), rapsyn, SFKs, α-dystrobrevin-2 (α-DB-2) and β-dystroglycan (β-DG) all partition efficiently into CRLMs. (E) Blots as shown in (D) were quantitated by densitometric scanning. For each protein, the intensities of bands in CRLM fractions 4–6 were related to the sum of all fractions to quantify the percentage in CRLMs (n=number of experiments). Download figure Download PowerPoint We probed fractions for the content of postsynaptic proteins. We found the AChR highly enriched in CRLMs, as 70% of the total receptor present in all fractions of the gradient resided in fractions 4–6 (Figure 5D and E). This reflects a 7.3-fold enrichment over bulk protein (Figure 5C). Like AChRs, elements of the agrin signaling pathway such as MuSK and rapsyn were similarly concentrated in CRLMs. The same was true for SFKs (Figure 5D and E), which are known from other cell types to be typical constituents of CRLMs (Resh, 1999; Simons and Toomre, 2000). Finally, β-dystroglycan and α-dystrobrevin-2, members of the utrophin complex important for NMJ stabilization (Grady et al, 2000; Jacobson et al, 2001), were also recovered efficiently in CRLMs. An overnight incubation with agrin, sufficient to produce maximal AChR clustering, did not detectably affect CRLM-association of AChR, rapsyn and MuSK (data not shown). Dispersion of CRLMs disrupts AChR clusters, AChR–rapsyn interaction and AChR β phosphorylation Besides microdomain-association of proteins, another standard tool to investigate the role of CRLMs in a given cellular process is to disrupt them by methyl-β-cyclodextrin (MβCD). We treated wild-type myotubes overnight with agrin to induce maximal AChR clusters and then added MβCD for 1–1.5 h. The number of clusters of normal size and morphology was strongly reduced by MβCD (Figure 6). On MβCD treatment, we noticed many smaller clusters and areas containing cluster fragments. The myotube morphology was unaffected, and following removal of MβCD the myotubes lived for extended periods of time and formed normal agrin-induced stable AChR clusters, like untreated controls (data not shown). This indicates that the MβCD effect was specific and not a consequence of impaired myotube health. These data thus show that the integrity of CRLMs is required to maintain the accumulation of large focal AChR clusters at the cell surface. Figure 6.MβCD disrupts AChR clusters, AChR–rapsyn interaction and AChR β phosphorylation in C2C12 myotubes. (A) C2C12 myotubes where first treated overnight with agrin (Ag) to induce AChR clusters. MβCD was then added in the continued presence of agrin, causing AChR clusters to fragment and disappear, as revealed by rhodamine-α-BT staining. (B) Clusters of 5 μM minimal size were quantitated. (C, D) Cells were treated with agrin and MbCD (5 mM, 1.5 h) as in (A). AChRs were precipitated from cell lysates using biotin-α-BT (Tox-P). In immunoblots, AChR-associated rapsyn (C), phosphorylation of AChR β (D) and AChR β itself (C, D; whole protein-control) were detected and quantified by densitometric scanning (C, n=4; D, n=8). *P<0.05, **P<0.01, by two-tailed t-test. Download figure Download PowerPoint Maintenance of the AChR–rapsyn interaction and of AChR β-phosphorylation depends on SFKs and is crucial in maintaining clusters (Sadasivam et al, 2005). We therefore analyzed the role of CRLMs in these processes. Myotubes were again treated overnight with agrin to induce maximal clustering, followed by addition of 5 mM MβCD for 1.5 h. AChRs were isolated from cell lysates, and associated rapsyn or phosphotyrosine content determined by immunoblotting. We found that the agrin-induced increase in AChR–rapsyn interaction was disrupted by MβCD (Figure 6C). Likewise, agrin-induced phosphorylation of AChR β was reduced to basal levels by MβCD (Figure 6D). Taken together, these results show that dispersion of CRLMs prevents the maintenance of large focal AChR clusters by disrupting agrin-induced AChR–rapsyn interaction and AChR phosphorylation. Impaired partitioning of postsynaptic proteins into CRLMs in the absence of Src and Fyn, and rescue by cholesterol To further define the molecular mechanism through which cholesterol and lipid microdomains stabilize AChR clusters, we analyzed the composition of CRLMs prepared from src−/−;fyn−/− myotubes, in which clusters are unstable. Like those from wild-type cells, CRLMs from mutant myotubes were enriched for ganglioside GM1, caveolin-3 and flotillin-2 (Figure 7A). A cholesterol profile revealed enrichment in CRLMs, but to a lesser extent than in wild-type cells (Figure 7B), as the CRLM peak was smaller (fractions 4–6) whereas non-CRLM fractions (e.g. 8) were increased (see also Figure 7E). CRLMs from src−/−;fyn−/− myotubes contained little overall protein (Figure 7C), as did wild-type CRLMs. Interestingly, significantly less of the total AChR and MuSK were in CRLMs from src−/−;fyn−/− myotubes when compared to wild type, the decrease being 30% for AChRs and 23% for MuSK (Figure 7D). Figure 7.In src−/−;fyn−/− myotubes, CRLM association of postsynaptic proteins is reduced but restored by cholesterol. (A–C) Characterization of CRLMs in src−/−;fyn−/− myotubes. CRLMs were prepared from clones DM11 or DM15, and the content, in gradient fractions, of ganglioside GM1, caveolin-3, flotillin-2 (A), cholesterol (B, n=12) and total protein (C, n=4) was analyzed. Markers are concentrated in CRLM fractions 4–6, with overall protein enriched at the gradient bottom (negative control). Cholesterol is less enriched in CRLMs than in wild-type cells (B; we show the profile from Figure 5B for comparison). (D) Gradient fractions were analyzed for the content of AChR and MuSK, and the percentage of these proteins in CRLM fractions 4–6 was quantified as in Figure 5E. Wild-type cells (C2C12 or clones SW5 and SW10; n=3–7), src−/−;fyn−/− myotubes (n=4–6) and src−/−;fyn−/− myotubes treated with cholesterol (n=4–5) were used. src−/−;fyn−/− myotubes have significantly lower percentages of AChRs and MuSK in CRLMs, and cholesterol restores this. (E) Cells were treated as in (D) and the percentage of cholesterol in CRLM fractions 4–6 was quantitated. CRLM association of cholesterol is lower in src−/−;fyn−/− myotubes (n=12) than in wild-type myotubes (n=8). Addition of cholesterol to the cell culture medium restores the amount of cholesterol in the CRLM fractions to the levels of wild-type myotubes (n=6). (F) Analysis as in (E), examining caveolin-3 (Cav). (G) The total amount of cholesterol, detected in total cell extracts, is the same in wild-type and src−/−;fyn−/− myotubes (n=8). *P<0.05, **P<0.01, by two-tailed t-test. Download figure Download PowerPoint The overproportionally decreased CRLM association of AChRs in src−/−;fyn−/− myotubes could have two reasons: Src and Fyn may maintain normal numbers of CRLMs in a myotube and/or act as a recruitment signal that brings postsynaptic proteins (such as AChRs) into CRLMs. To investigate these possibilities, we quantitated CRLM partitioning of typical CRLM markers. 19% less of total cholesterol were found in the CRLM fractions 4–6 in src−/−;fyn−/− myotubes when compared to wild type (Figure 7E) and similar observations were made for caveolin-3 (Figure 7F). Overall cellular levels of cholesterol, quantified per microgram of cellular protein, were normal in the mutants, excluding overall nonspecific effects from the lack of Src and Fyn (Figure 7G). These data suggest that src−/−;fyn−/− myotubes have less CRLMs than wild-type cells. The reduction in CRLMs however appears smaller than the reduction in CRLM association of AChRs. Thus, Src and Fyn most likely also act as a recruitment factor for AChRs (and MuSK) into CRLMs. Importantly, cholesterol addition to src−/−;fyn−/− myotubes not only stabilized AChR clusters (Figure 3), but restored the CRLM partitioning of AChRs and MuSK back to normal (Figure 7D). Likewise, the CRLM enrichment of cholesterol itself and of caveolin were normalized by cholesterol treatment (Figure 7E and F). Thus, while the absence of Src and Fyn decreases the number of C