Publication Information

{'id': 'https://openalex.org/W2147718057', 'doi': 'https://doi.org/10.1074/jbc.m109078200', 'title': 'UBP43 (USP18) Specifically Removes ISG15 from Conjugated Proteins', 'display_name': 'UBP43 (USP18) Specifically Removes ISG15 from Conjugated Proteins', 'publication_year': 2002, 'publication_date': '2002-03-01', 'ids': {'openalex': 'https://openalex.org/W2147718057', 'doi': 'https://doi.org/10.1074/jbc.m109078200', 'mag': '2147718057', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/11788588'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m109078200', 'pdf_url': 'http://www.jbc.org/article/S002192581936123X/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'hybrid', 'oa_url': 'http://www.jbc.org/article/S002192581936123X/pdf', 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5010807256', 'display_name': 'Michael P. 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3517, 3546, 3617, 3729, 3763, 3767, 3823, 3833, 3946, 4086, 4140, 4162, 4167, 4204, 4232, 4320, 4395, 4423], 'previously': [10, 169, 1789, 2031, 2822], 'was': [11, 30, 170, 189, 464, 548, 670, 1204, 1272, 1588, 1598, 1971, 1978, 2002, 2061, 2084, 2106, 2161, 2273, 2313, 2325, 2344, 2446, 2467, 2479, 2520, 2568, 2675, 2796, 2814, 2894, 2905, 2919, 2941, 3136, 3187, 3247, 3271, 3300, 3322, 3378, 3399, 3420, 3458, 3476, 3529, 3662, 3861, 3978, 4017, 4091, 4344, 4354], 'shown': [12, 171], 'hydrolyze': [14, 173, 4147, 4179, 4184, 4407], 'ubiquitin-β-galactosidase': [15, 174], 'fusions': [16, 29, 62, 175, 188, 221, 1667, 2121, 2280, 2390, 2435, 2531, 2558, 2687, 2971, 3840, 4287, 4375, 4409], 'in': [17, 32, 92, 102, 153, 176, 191, 251, 261, 312, 682, 837, 947, 1070, 1083, 1177, 1207, 1274, 1341, 1375, 1383, 1421, 1439, 1469, 1668, 1671, 1752, 1784, 2135, 2235, 2251, 2356, 2407, 2418, 2469, 2503, 2576, 2739, 2816, 2914, 2932, 2991, 2995, 3002, 3020, 3032, 3041, 3049, 3119, 3143, 3222, 3274, 3401, 3509, 3550, 3593, 3670, 3732, 3745, 3849, 3944, 4000, 4100, 4169, 4202, 4227, 4241, 4267, 4332, 4349, 4379], 'Escherichia': [18, 177, 1572], 'coli.': [19, 178], 'In': [20, 71, 179, 230, 4243], 'our': [21, 180, 1208, 1656], 'assays,': [22, 181], 'the': [23, 38, 89, 114, 182, 197, 248, 273, 341, 543, 826, 916, 925, 976, 1042, 1051, 1060, 1076, 1094, 1163, 1390, 1427, 1440, 1483, 1513, 1516, 1579, 1672, 1689, 1697, 1725, 1746, 1816, 1968, 1975, 1999, 2066, 2082, 2098, 2120, 2132, 2209, 2218, 2221, 2236, 2252, 2271, 2301, 2331, 2410, 2444, 2557, 2623, 2721, 2731, 2892, 2917, 2944, 2965, 3014, 3102, 3265, 3390, 3447, 3485, 3488, 3506, 3679, 3721, 3733, 3743, 3759, 3820, 3826, 3835, 3851, 3964, 3992, 4003, 4020, 4110, 4116, 4125, 4143, 4237, 4278, 4418, 4421, 4424], 'activity': [24, 183, 1488, 1593, 1662, 1765, 2813, 3549, 3590, 3607, 3856, 4218, 4226, 4239, 4284], 'of': [25, 40, 88, 99, 150, 184, 199, 247, 258, 309, 334, 340, 545, 606, 709, 828, 919, 960, 971, 1033, 1041, 1050, 1054, 1059, 1078, 1133, 1137, 1281, 1389, 1424, 1429, 1448, 1515, 1568, 1663, 1675, 1699, 1724, 1766, 1775, 1908, 1954, 2026, 2065, 2094, 2122, 2159, 2165, 2176, 2224, 2232, 2243, 2254, 2422, 2426, 2440, 2458, 2486, 2537, 2564, 2602, 2640, 2672, 2693, 2705, 2742, 2770, 2776, 2787, 2924, 2949, 2967, 2989, 3011, 3017, 3026, 3034, 3037, 3052, 3080, 3105, 3128, 3134, 3146, 3208, 3225, 3264, 3277, 3351, 3396, 3413, 3462, 3487, 3539, 3548, 3591, 3598, 3608, 3678, 3723, 3819, 3825, 3847, 3887, 3904, 3937, 3974, 3994, 4002, 4115, 4132, 4154, 4174, 4223, 4275, 4305, 4313, 4362, 4369, 4391], 'toward': [27, 186, 1665, 3610, 3863, 4219], 'Ub': [28, 187, 797, 952, 961, 1454, 1595, 1603, 1666, 2123, 2143, 3377, 3599, 3945, 4224, 4286, 4314, 4374, 4408, 4422], 'undetectable': [31, 190, 3769], 'vitro': [33, 192, 3594, 4350, 4380], 'directing': [34, 193], 'us': [35, 194, 1681, 3604], 'investigate': [37, 196], 'possibility': [39, 198, 4153], 'Ub-like': [41, 200, 1707], 'proteins': [42, 152, 201, 311, 677, 711, 926, 964, 973, 1466, 1708], 'such': [43, 202, 2998, 4372], 'as': [44, 49, 203, 208, 601, 974, 1584, 1858, 1860, 2354, 2430, 2448, 2821, 2999, 3319, 3404, 3685, 3765, 4191, 4373], 'SUMO,': [45, 204, 689, 1710, 3615], 'Nedd8,': [46, 205, 830, 1711, 3616], 'ISG15': [48, 61, 65, 100, 122, 207, 220, 224, 259, 281, 337, 546, 591, 669, 786, 822, 920, 931, 940, 955, 1034, 1055, 1065, 1085, 1095, 1156, 1676, 1685, 1703, 1734, 2125, 2145, 3332, 4123, 4316], 'probable': [50, 209], 'substrates.': [51, 210, 1716, 4294], 'We': [52, 138, 211, 297, 1694, 4295], 'consequently': [53, 212, 1695], 'demonstrate': [54, 213, 589, 1719], 'that': [55, 105, 140, 214, 264, 299, 590, 785, 817, 927, 1029, 1048, 1162, 1416, 1565, 1684, 1720, 1757, 1983, 2147, 3957, 3973, 4120, 4208, 4214, 4403], 'can': [57, 216, 599, 942], 'efficiently': [58, 217, 3892, 4195, 4328], 'cleave': [59, 218, 1702, 4413], 'only': [60, 219, 3831], 'including': [63, 222, 1426, 3907], 'native': [64, 223, 1733], 'conjugates': [66, 101, 225, 260, 941, 1035, 1604, 1686], 'linked': [67, 226], 'via': [68, 227, 678, 789], 'isopeptide': [69, 228, 680, 4415], 'bonds.': [70, 229], 'addition': [72, 231], 'commonly': [74, 233, 4383], 'used': [75, 234, 2308, 2339, 2416, 2781, 3346, 3383, 3736, 4384, 4394], 'methods': [76, 235], 'we': [77, 236, 1718, 3584, 3735, 3873, 4157], 'introduce': [78, 237], 'a': [79, 96, 108, 146, 238, 255, 267, 305, 555, 683, 714, 790, 948, 1030, 1102, 1138, 1141, 1152, 1275, 1342, 1376, 1422, 1562, 1585, 1722, 1760, 2078, 2166, 2669, 2678, 2817, 3132, 3219, 3348, 3405, 3433, 3596, 3674, 3749, 3967, 4152, 4159, 4244, 4265, 4382], 'new': [80, 239], 'experimental': [81, 240, 4155], 'design': [82, 241], 'featuring': [83, 242], 'ISG15-UBP43': [84, 243, 2970, 4321], 'fusion': [85, 244, 1583, 1783, 1998, 2312, 2343, 2450, 3407, 3474, 3555, 3752, 3895, 4134, 4325], 'self-processing.': [86, 245], 'Deletion': [87, 246], 'gene': [91, 250, 320, 1392, 2069, 2234], 'mouse': [93, 252], 'leads': [94, 253], 'massive': [97, 256, 3006], 'increase': [98, 257], 'tissues': [103, 262], 'indicating': [104, 263], 'is': [107, 113, 142, 266, 272, 301, 338, 389, 592, 823, 937, 975, 1045, 1057, 1086, 1098, 1159, 1166, 1745, 1759, 1769, 3857, 4142, 4210, 4249, 4269, 4381, 4393], 'major': [109, 268, 1761, 3612], 'ISG15-specific': [110, 118, 269, 277, 1748, 1762, 4212, 4238], 'protease.': [111, 270], 'first': [115, 274, 1747, 3507, 4144], 'bona': [116, 275], 'fide': [117, 276], 'protease': [119, 278, 327, 1727, 1749, 1763, 2289, 2329, 3157, 4213], 'reported.': [120, 279, 1750], 'Both': [121, 280], 'andUBP43': [123, 282], 'genes': [124, 283, 345, 1379, 1679, 2146], 'are': [125, 284, 928, 1036, 4258, 4291, 4398, 4404, 4410], 'known': [126, 285, 1706], 'be': [128, 287, 673, 943, 1101, 1419, 1688, 3780, 4308, 4327, 4340], 'strongly': [129, 288, 343, 4206], 'induced': [130, 289, 344, 392, 1380, 2274, 2480, 3062], 'by': [131, 290, 393, 467, 488, 594, 930, 1181, 1263, 1381, 1393, 1399, 1887, 2063, 2130, 2163, 2173, 2346, 2494, 2501, 2522, 2668, 2677, 2798, 2810, 2943, 3075, 3138, 3189, 3249, 3309, 3336, 3445, 3624, 3726, 3817, 3960, 3986, 4022, 4093, 4317], 'interferon,': [132, 291], 'genotoxic': [133, 292, 443], 'stress,': [134, 293, 1433], 'viral': [136, 295, 1394], 'infection.': [137, 296], 'postulate': [139, 298], 'necessary': [143, 302], 'maintain': [145, 304], 'critical': [147, 306, 2167, 3854], 'cellular': [148, 307, 1773], 'balance': [149, 308, 1774], 'ISG15-conjugated': [151, 310, 1776], 'both': [154, 313, 1730, 2334], 'healthy': [155, 314], 'stressed': [157, 316], 'organisms.': [158, 317], 'interferon': [318, 347], 'interferon-stimulated': [319], '15': [321], 'kDa': [322], 'ubiquitin': [323, 687], 'ubiquitin-like': [324, 710], 'protein(s)': [325], 'glutathione-S-transferase': [328], 'phosphate-buffered': [329], 'saline': [330], 'matrix-assisted': [331, 2935], 'laser': [332, 2936], 'desorption/ionization-time': [333], 'flight': [335], 'N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine': [336], 'one': [339, 1049, 4303, 4311], 'most': [342], 'after': [346, 1062, 2628, 3078, 3505, 3756, 3940], '(IFN)1': [348], 'treatment': [349], '(1Farrell': [350], 'P.J.': [351, 471], 'Broeze': [352, 472], 'R.J.': [353, 473, 652, 3359], 'Lengyel': [354, 474], 'P.': [355, 421, 475, 570], 'Nature.': [356, 476, 3806], '1979;': [357, 477], '279:': [358, 478], '523-525Crossref': [359, 479], 'PubMed': [360, 383, 406, 437, 459, 480, 512, 539, 584, 642, 705, 739, 754, 779, 811, 846, 859, 875, 895, 911, 989, 1005, 1023, 1126, 1199, 1232, 1255, 1298, 1331, 1367, 1496, 1536, 1556, 1627, 1650, 1809, 1837, 1853, 1881, 1936, 2055, 2203, 2383, 2843, 2877, 3579, 3657, 3711, 3794, 3810, 3930, 4047, 4081], 'Scopus': [361, 384, 407, 438, 460, 481, 643, 740, 755, 780, 812, 847, 860, 876, 896, 912, 990, 1006, 1024, 1127, 1200, 1233, 1256, 1299, 1332, 1368, 1497, 1537, 1557, 1628, 1651, 1810, 1854, 1937, 2056, 2204, 2384, 2844, 2878, 3580, 3658, 3712, 3795, 3811, 3931, 4048, 4082], '(203)': [362, 482], 'Google': [363, 386, 409, 440, 462, 483, 513, 540, 585, 619, 645, 665, 706, 742, 757, 782, 814, 849, 862, 878, 898, 914, 992, 1008, 1026, 1129, 1202, 1235, 1258, 1301, 1334, 1370, 1499, 1523, 1539, 1559, 1630, 1653, 1812, 1838, 1856, 1882, 1939, 2058, 2206, 2386, 2846, 2880, 3372, 3582, 3660, 3714, 3797, 3813, 3933, 4050, 4084], 'Scholar,': [364, 514, 620, 646, 743, 850, 863, 879, 899, 993, 1236, 1500, 1524, 1540, 1631, 2847, 4051], '2Der': [365], 'S.D.': [366], 'Zhou': [367, 1241, 1636, 1795], 'A.': [368, 379, 638, 745, 747, 1218, 1613, 1827, 1925, 1949, 2041, 2189, 2372, 3565, 3700], 'Williams': [369], 'B.R.': [370], 'Silverman': [371], 'R.H.': [372], 'Proc.': [373, 632], 'Natl.': [374, 633], 'Acad.': [375, 634], 'Sci.': [376, 635], 'U.': [377, 636], 'S.': [378, 446, 637, 650, 881, 1359, 3357], '1998;': [380], '95:': [381], '15623-15628Crossref': [382], '(1544)': [385], 'Scholar)': [387, 484, 783, 815, 1027, 1259, 1335, 1371, 1857, 1883, 3661, 3798, 3934, 4085], 'also': [390, 1148, 1755], 'significantly': [391], 'influenza': [394], 'B': [395, 3977], 'virus': [396], '(3Yuan': [397, 802], 'W.': [398, 803, 1315], 'Krug': [399, 801, 804], 'R.M.': [400, 805], 'EMBO': [401, 806], 'J.': [402, 413, 426, 453, 503, 530, 575, 614, 622, 648, 660, 696, 763, 768, 807, 901, 1110, 1115, 1223, 1287, 1320, 1492, 1618, 1828, 1869, 1872, 1930, 2046, 2194, 2377, 2827, 2832, 2866, 3355, 3367, 3570, 3646, 3705, 3914, 3919, 4031, 4036, 4070], '2001;': [403, 429, 736, 808, 1364], '20:': [404, 809], '362-371Crossref': [405, 810], '(422)': [408, 813], 'Scholar),': [410, 441, 915, 1302, 1839, 3583], 'lipopolysaccharide': [411, 1402], '(4Li': [412], 'Peet': [414], 'G.W.': [415], 'Balzarano': [416], 'D.': [417, 495, 519, 529, 1353, 1510], 'Li': [418, 1303], 'X.': [419, 1285], 'Massa': [420], 'Barton': [422], 'R.W.': [423], 'Marcu': [424], 'K.B.': [425], 'Biol.': [427, 504, 531, 576, 697, 769, 855, 886, 907, 1019, 1116, 1228, 1321, 1623, 1829, 1873, 2051, 2199, 2833, 2867, 3575, 3647, 3920, 4037, 4071], 'Chem.': [428, 505, 532, 577, 698, 770, 1117, 1322, 1830, 1874, 2834, 2868, 3648, 3921, 4038, 4072], '276:': [430], '18579-18590Abstract': [431], 'Full': [432, 434, 509, 536, 581, 702, 774, 776, 890, 892, 1121, 1123, 1326, 1328, 1834, 1878, 2838, 2840, 2872, 2874, 3652, 3654, 3925, 3927, 4042, 4044, 4076, 4078], 'Text': [433, 435, 510, 537, 582, 703, 775, 777, 891, 893, 1122, 1124, 1327, 1329, 1835, 1879, 2839, 2841, 2873, 2875, 3653, 3655, 3926, 3928, 4043, 4045, 4077, 4079], 'PDF': [436, 511, 538, 583, 704, 778, 894, 1125, 1330, 1836, 1880, 2842, 2876, 3656, 3929, 4046, 4080], '(135)': [439], 'stress': [444], '(5Memet': [445], 'Besancon': [447], 'F.': [448], 'Bourgeade': [449], 'M.F.': [450], 'Thang': [451], 'M.N.': [452], 'Interferon': [454], 'Res.': [455, 1531], '1991;': [456, 616, 1831], '11:': [457, 1494], '131-141Crossref': [458], '(45)': [461], 'Scholar).ISG15': [463], 'originally': [465, 3622], 'identified': [466, 1336], 'Farrell': [468], 'et': [469, 760, 1183, 1304, 1350, 2358, 3626], 'al.(1Farrell': [470], 'later': [486], 'Knight': [489, 500, 623, 655, 3362], 'co-workers': [491], '(6Blomstrom': [492], 'D.C.': [493], 'Fahey': [494, 518], 'Kutny': [496, 524], 'R.': [497, 525, 1214, 1609, 1867, 1889, 2037, 2185, 3561], 'Korant': [498], 'B.D.': [499], 'Jr.,': [501, 516, 610, 624, 656, 1213, 1608, 2036, 2184, 3363, 3560], 'E.': [502, 517, 611, 625, 657, 1190, 2268, 2460, 2992, 3018, 3044, 3338, 3364, 3402, 3551, 3671, 4228, 4333], '1986;': [506], '261:': [507], '8811-8816Abstract': [508], '7Knight': [515], 'Cordova': [520, 612], 'B.': [521, 613], 'Hillman': [522], 'M.': [523, 841, 852, 984, 1313, 1405, 1504, 1927, 2374, 3702], 'Reich': [526], 'N.': [527, 1542], 'Blomstrom': [528], '1988;': [533], '263:': [534], '4520-4522Abstract': [535], 'Scholar).': [541, 586, 666, 707, 758, 1009, 1130, 1560, 1654, 1813, 1940, 2059, 2387, 2881, 3373, 3715, 3814], 'Subsequently,': [542], 'sequence': [544, 1442, 2001, 2021, 3681], 'protein': [547, 2477, 3130, 3434, 3475, 3556], 'noted': [549], 'possess': [551], 'diubiquitin': [556], 'sequence,': [557, 1474], 'accounting': [558], 'for': [559, 821, 1154, 1269, 1338, 1344, 1378, 1413, 1486, 1575, 1581, 1692, 1771, 1780, 2309, 2340, 2404, 2695, 2782, 3065, 3182, 3194, 3251, 3524, 3664, 3855, 3953], 'its': [560, 3775, 4087], 'cross-reactivity': [561], 'with': [562, 796, 978, 1038, 1171, 2097, 2111, 2126, 2138, 2142, 2275, 2287, 2319, 2327, 2393, 2409, 2464, 2481, 2497, 2544, 2570, 2614, 2633, 2702, 2720, 2730, 2733, 2801, 2888, 2909, 2978, 3063, 3099, 3261, 3415, 3870, 3877, 3996, 4007, 4025, 4109, 4230, 4348], 'affinity': [563], 'purified': [564, 2282, 2314, 2345, 2895, 3409, 3431, 3889], 'anti-ubiquitin': [565], 'antibodies': [566, 2803, 3100, 3389, 3442, 3464], '(8Haas': [567], 'A.L.': [568, 627, 695, 749, 767, 1016, 1114, 2831, 3918, 4035], 'Ahrens': [569], 'Bright': [571], 'P.M.': [572], 'Ankel': [573], 'H.': [574, 1238, 1355, 1633, 1792, 1921, 1923, 2368, 2370, 3696, 3698, 3785], '1987;': [578, 3791], '262:': [579, 1296], '11315-11323Abstract': [580], 'Several': [587, 1437], 'reports': [588], 'released': [593, 2807, 4015, 4117], 'various': [595], 'cell': [596, 1385, 1430, 2609], 'types': [597], 'act': [600], 'cytokine': [602], 'leading': [603], 'proliferation': [605], 'NK': [607], 'cells': [608, 1786, 2491, 2604, 2630, 3876], '(9Knight': [609], 'Immunol.': [615, 661, 3368], '146:': [617], '2280-2284PubMed': [618], "10D'Cunha": [621], 'Haas': [626, 694, 766, 1012, 1015, 1113, 1951, 2830, 3917, 4034], 'Truitt': [628], 'R.L.': [629], 'Borden': [630, 658, 3339, 3365], 'E.C.': [631, 659, 3366], '1996;': [639, 662, 771, 3369], '93:': [640], '211-215Crossref': [641], '(299)': [644], "11D'Cunha": [647], 'Ramanujam': [649, 3356], 'Wagner': [651, 3358], 'Witt': [653, 3360], 'P.L.': [654, 3361], '157:': [663, 3370], '4100-4108PubMed': [664, 3371], 'Most': [667], 'remarkably,': [668], 'found': [671], 'conjugated': [674, 1456], 'intracellular': [676], 'an': [679, 818, 1145, 1155, 4170, 4211, 4389, 4414], 'bond': [681, 4419], 'manner': [684], 'similar': [685, 791, 950, 3971], '(Ub),': [688], 'Nedd8': [691, 979, 1079, 1977], '(12Loeb': [692], 'K.R.': [693, 1014], '1992;': [699], '267:': [700, 1365], '7806-7813Abstract': [701], 'Conjugation': [708], '(Ubls)': [712], 'involves': [713, 1566], 'three-step': [715], 'mechanism': [716], 'whereby': [717], 'specific': [718, 1740, 4225], 'enzymes': [719], '(or': [720, 1396], 'enzyme': [721, 820, 1577, 1768, 3910, 4145, 4276, 4392], 'complexes)': [722], 'activate': [723], 'covalently': [725], 'link': [726], 'Ubls': [727, 3724], 'their': [729], 'substrates': [730, 1691, 3938, 4371], '(13Pickart': [731], 'C.M.': [732, 2857, 3637, 4061], 'Annu.': [733], 'Rev.': [734], 'Biochem.': [735, 1529, 1931, 2378, 3706, 3790], '70:': [737], '503-533Crossref': [738], '(2944)': [741], '14Ciechanover': [744], 'Orian': [746], 'Schwartz': [748], 'Bioessays.': [750], '2000;': [751, 843, 856, 872, 887, 908, 986, 1002, 1252, 1323, 1553, 1647, 1806, 1850], '22:': [752], '442-451Crossref': [753], '(706)': [756], 'Narasimhan': [759, 1109, 2826, 3913, 4030], 'al.': [761, 1184, 1305, 1351, 3627], '(15Narasimhan': [762], 'Potter': [764], 'J.L.': [765, 1108, 2825, 3912, 4029], '271:': [772], '324-330Abstract': [773], '(125)': [781], 'demonstrated': [784, 1028, 1589], 'conjugation': [787, 833, 956], 'occurs': [788, 3962], 'but': [792, 3005, 3799], 'distinct': [793], 'pathway': [794, 1097], 'compared': [795], 'conjugation.': [798, 3954], 'Yuan': [799], 'discovered': [816], 'activating': [819], 'UBE1L.': [824], 'Although': [825], 'role': [827], 'Ub,': [829], 'SUMO': [832, 981, 1081], 'has': [834, 1787, 2029, 4135], 'been': [835, 934, 1068, 1788, 1985, 2023, 2030, 2149, 4137], 'assessed': [836], 'numerous': [838], 'studies': [839], '(16Hochstrasser': [840, 983], 'Science.': [842, 985], '289:': [844, 987], '563-564Crossref': [845, 988], '(102)': [848, 991], '17Hochstrasser': [851], 'Nat.': [853], 'Cell': [854, 885], '2:': [857], 'E153-E157Crossref': [858], '(370)': [861], '18Yeh': [864, 994], 'E.T.': [865, 995], 'Gong': [866, 996], 'L.': [867, 997, 1112, 1242, 1637, 1796, 1950, 2829, 3916, 4033], 'Kamitani': [868, 998], 'T.': [869, 999, 1913, 1915, 2360, 2362, 3688, 3690], 'Gene': [870, 1000, 1362], '(Amst.).': [871, 1001, 1363], '248:': [873, 1003], '1-14Crossref': [874, 1004], '(420)': [877, 1007], '19Jentsch': [880], 'Pyrowolakis': [882], 'G.': [883, 3788], 'Trends': [884], '10:': [888], '335-342Abstract': [889], '(294)': [897], '20Callis': [900], 'Vierstra': [902, 1549], 'R.D.': [903, 1550], 'Curr.': [904], 'Opin.': [905], 'Plant': [906, 1551], '3:': [909], '381-386Crossref': [910], '(170)': [913], 'biological': [917, 969], 'significance': [918], 'modification': [921, 982, 1056], 'remains': [922], 'unknown': [923, 938], 'targeted': [929, 944, 972], 'have': [932, 1067, 1591], 'not': [933, 1150, 3586, 3800, 3834, 3900, 4188, 4292, 4400], 'identified.': [935], 'It': [936, 1044, 1158], 'whether': [939], 'proteasomes': [946], 'way': [949], 'conjugates.': [953, 1735], 'Alternatively,': [954], 'might': [957, 1418], 'antagonize': [958], 'binding': [959], 'save': [963], 'from': [965, 1088, 1278, 1455, 1714, 1900, 1947, 1988, 2004, 2017, 2152, 2246, 2808, 3111, 3213, 3380, 3530, 4019, 4251, 4260], 'degradation': [966, 3010], 'or': [967, 1965, 1981, 1990, 2124, 2144, 2154, 2433, 2984, 3838, 3880, 4186, 4315, 4359], 'modify': [968], 'activities': [970], 'case': [977], 'Loeb': [1010], '(21Loeb': [1013], 'Mol.': [1017, 1226, 1621, 2049, 2197, 3573], 'Cell.': [1018, 1227, 1517, 1622, 2050, 2198, 3574], '1994;': [1020, 1875], '14:': [1021], '8408-8419Crossref': [1022], '(92)': [1025], 'substantial': [1031], 'amount': [1032], 'co-localized': [1037], 'intermediate': [1039], 'filaments': [1040], 'cytoskeleton.': [1043], 'therefore': [1046, 1160, 3768, 4296], 'possible': [1047, 1161, 3730], 'physiological': [1052], 'roles': [1053], 're-organization': [1058], 'cytoskeleton': [1061], 'IFN': [1063, 1173, 1382, 1400], 'stimulation.': [1064], 'sequences': [1066], 'reported': [1069, 4138], 'fish,': [1071], 'chick,': [1072], 'mammals.': [1074], 'Despite': [1075, 3536], 'presence': [1077], 'analogs': [1082], 'yeast,': [1084], 'absent': [1087], 'this': [1089, 1767, 2255, 3042, 3665, 3868, 4101], 'unicellular': [1090], 'eukaryote': [1091], 'and,': [1092, 4346], 'therefore,': [1093], 'regulatory': [1096], 'suggested': [1099], 'relatively': [1103], 'recent': [1104], 'functional': [1105, 3949], 'divergence': [1106], '(22Potter': [1107, 2824, 3911, 4028], 'Mende-Mueller': [1111, 2828, 3915, 4032], '1999;': [1118, 1196, 1229, 1295, 1533, 1624, 1933, 2052, 2200, 2380, 2835, 3576, 3708, 3922, 4039], '274:': [1119, 2836, 3923, 4040], '25061-25068Abstract': [1120, 2837, 3924, 4041], '(84)': [1128, 2845, 3932, 4049], 'Our': [1131], 'search': [1132], 'fully': [1134], 'sequenced': [1135], 'genomes': [1136], 'nematode': [1139], '(Caenorabditis),': [1140], 'plant': [1142], '(Arabidopsis),': [1143], 'insect': [1146], '(Drosophila)': [1147], 'did': [1149, 3899], 'reveal': [1151], 'candidate': [1153], 'ortholog.': [1157], 'ISG15-conjugation': [1164], 'system': [1165, 3528], 'restricted': [1167], 'higher': [1169], 'animals': [1170], 'evolved': [1172], 'signaling.': [1174], '(USP18': [1176], 'standard': [1178, 4245], 'nomenclature': [1179], 'proposed': [1180], 'Baker': [1182, 1845, 1890], '(23Baker': [1185], 'R.T.': [1186, 1846, 1863], 'Wang': [1187], 'X.W.': [1188], 'Woollatt': [1189], 'White': [1191], 'J.A.': [1192, 1309], 'Sutherland': [1193], 'G.R.': [1194], 'Genomics.': [1195, 1251, 1646, 1805], '59:': [1197], '264-274Crossref': [1198], '(64)': [1201], 'Scholar))': [1203, 2207], 'initially': [1205], 'cloned': [1206, 1273, 1372, 2011, 2107], 'laboratory': [1209], '(24Liu': [1210, 1605, 2033, 2181, 3557], 'L.Q.': [1211, 1240, 1606, 1635, 1794, 2034, 2182, 3558], 'Ilaria': [1212, 1607, 2035, 2183, 3559], 'Kingsley': [1215, 1610, 2038, 2186, 3562], 'P.D.': [1216, 1611, 2039, 2187, 3563], 'Iwama': [1217, 1612, 2040, 2188, 3564], 'van': [1219, 1614, 2042, 2190, 3566], 'Etten': [1220, 1615, 2043, 2191, 3567], 'R.A.': [1221, 1616, 2044, 2192, 3568], 'Palis': [1222, 1617, 2045, 2193, 3569], 'Zhang': [1224, 1249, 1619, 1644, 1803, 2047, 2195, 3571], 'D.E.': [1225, 1250, 1620, 1645, 1804, 2048, 2196, 3572], '19:': [1230, 1625, 2053, 2201, 3577], '3029-3038Crossref': [1231, 1626, 2054, 2202, 3578], '(132)': [1234, 1629, 2057, 2205, 3581], '25Schwer': [1237, 1632], 'Liu': [1239, 1312, 1634, 1793], 'Little': [1243, 1638, 1797], 'M.T.': [1244, 1639, 1798], 'Pan': [1245, 1640, 1799], 'Z.': [1246, 1641, 1800], 'Hetherington': [1247, 1642, 1801], 'C.J.': [1248, 1643, 1802], '65:': [1253, 1648, 1807], '44-52Crossref': [1254, 1649, 1808], '(57)': [1257, 1652, 1811], 'later,': [1261], 'independently': [1262], 'three': [1264, 3214, 4176, 4194], 'other': [1265, 1705, 1737, 3611, 4220], 'groups;': [1266], 'cDNA': [1267], 'coding': [1268, 1574], 'porcine': [1270], 'differential': [1276], 'screen': [1277, 1343, 1377], 'lung': [1279], 'macrophages': [1280], 'virus-infected': [1282], 'swine': [1283], '(26Zhang': [1284], 'Shin': [1286], 'Molitor': [1288], 'T.W.': [1289], 'Schook': [1290], 'L.B.': [1291], 'Rutherford': [1292], 'M.S.': [1293, 2859, 3639, 4063], 'Virology.': [1294], '152-162Crossref': [1297], '(73)': [1300], '(27Li': [1306], 'X.L.': [1307], 'Blackford': [1308], 'Judge': [1310], 'C.S.': [1311], 'Xiao': [1314], 'Kalvakolanu': [1316], 'D.V.': [1317], 'Hassel': [1318], 'B.A.': [1319], '275:': [1324], '8880-8888Abstract': [1325], '(103)': [1333], 'mRNA': [1337], 'human': [1339, 1373, 3331], 'RNase': [1345], 'L': [1346], 'substrates,': [1347], 'Kang': [1349, 2858, 3638, 4062], '(28Kang': [1352], 'Jiang': [1354], 'Wu': [1356], 'Q.': [1357], 'Pestka': [1358], 'Fisher': [1360], 'P.B.': [1361], '233-242Crossref': [1366], '(60)': [1369], 'melanoma': [1384], 'lines.': [1386], 'Significant': [1387], 'up-regulation': [1388], 'infection': [1395], 'double-stranded': [1397], 'RNA),': [1398], '2O.': [1403], 'Malakhova,': [1404], 'Malakhov,': [1406], 'C.': [1407], 'Hetherington,': [1408], 'D.-E.': [1410], 'Zhang,': [1411], 'submitted': [1412], 'publication.': [1414], 'suggests': [1415], 'involved': [1420], 'number': [1423, 3162, 4253, 4262], 'processes': [1425], 'control': [1428], 'proliferation,': [1431], 'inflammation,': [1432], 'immune': [1435], 'response.': [1436], 'regions': [1438], 'exhibit': [1443], 'catalytic': [1446, 1487], 'domains': [1447], 'Ub-specific': [1449, 1726, 3544], '(USPs)': [1451], 'which': [1452, 1467, 2018, 2886, 3472, 3739, 3850, 4280], 'remove': [1453, 2883], 'proteins.': [1457, 1777], 'The': [1458, 2102, 2259, 2388, 2413, 2566, 2761, 2805, 2950, 3268, 3298, 3454, 3844, 3884, 4014, 4096, 4130, 4199], 'USP': [1459, 1576, 3905], 'family': [1460, 3906], 'may': [1461, 1687, 4281], 'include': [1462], 'more': [1463], 'than': [1464, 2438, 3008], '100': [1465, 3234, 3285], 'differ': [1468], 'size': [1470], 'amino': [1472], 'acid': [1473, 2913, 4027], 'yet': [1475], 'all': [1476, 4193, 4401], 'share': [1477], 'several': [1478], 'highly': [1479], 'homologous': [1480], 'patches': [1481], 'around': [1482], 'residues': [1484], 'required': [1485], '(29Wilkinson': [1489], 'K.D.': [1490, 1502], 'FASEB': [1491], '1997;': [1493], '1245-1256Crossref': [1495], '(511)': [1498], '30Wilkinson': [1501], 'Hochstrasser': [1503], 'Peters': [1505], 'J.M.': [1506], 'Harris': [1507], 'J.R.': [1508], 'Finley': [1509], 'Ubiquitin': [1511], 'Biology': [1514], 'Plenum': [1518], 'Press,': [1519], 'New': [1520], 'York1998:': [1521], '99-125Crossref': [1522], '31Chung': [1525], 'C.H.': [1526, 1919, 2366, 2865, 3645, 3694, 4069], 'Baek': [1527, 1916, 2363, 3691], 'S.H.': [1528, 1917, 2364, 3692], 'Biophys.': [1530, 1848], 'Commun.': [1532], '266:': [1534, 1832], '633-640Crossref': [1535], '(157)': [1538], '32Yan': [1541], 'Doelling': [1543], 'J.H.': [1544], 'Falbel': [1545], 'T.G.': [1546], 'Durski': [1547], 'A.M.': [1548], 'Physiol.': [1552], '124:': [1554], '1828-1843Crossref': [1555], '(110)': [1558], 'Using': [1561], 'conventional': [1563], 'assay': [1564, 3621, 3734, 4300], 'co-transformation': [1567, 4246], 'two': [1569, 4163], 'plasmids': [1570, 1898, 1944, 2260, 2616], 'into': [1571, 2012, 2070, 2108, 2171, 2226, 2266, 2637, 2921], 'coli(first': [1573], 'second': [1580], 'Ub-β-galactosidase': [1582, 3554, 4231, 4248], 'substrate),': [1586], 'proteolytic': [1592, 2812, 4217, 4283], 'against': [1594, 3101, 3330, 3376], 'fusion,': [1596], 'thought': [1599], 'work': [1601, 2256, 2956], 'on': [1602, 2283, 2315, 2534, 2607, 2896, 2954, 3092, 3124, 3180, 3410, 3432, 3465, 3770, 3782, 3801, 3890, 3981, 4285], 'However,': [1655], 'subsequent': [1657], 'experiments': [1658], 'failed': [1659], 'detect': [1661, 3589], 'vitro.': [1669], 'Similarity': [1670], 'expression': [1673, 1781, 2272, 2478, 3027, 4268], 'patterns': [1674], 'lead': [1680], 'hypothesize': [1683], 'preferred': [1690, 4293], 'UBP43.': [1693, 2442, 3012, 3039, 3106, 3883], 'investigated': [1696], 'ability': [1698, 2966], '(namely': [1709], 'Ub)': [1713], 'artificial': [1715, 1731, 4370], 'Here': [1717], 'UBP43,': [1721, 3848], 'member': [1723], 'family,': [1728], 'cleaves': [1729, 4122], 'No': [1736, 4337], 'USPs': [1738, 4165, 4177, 4257, 4402], 'were': [1739, 1885, 1899, 1945, 2128, 2239, 2257, 2264, 2281, 2306, 2391, 2492, 2532, 2559, 2594, 2605, 2631, 2688, 2699, 2737, 2779, 2976, 3047, 3072, 3090, 3108, 3168, 3178, 3199, 3216, 3333, 3345, 3430, 3443, 3481, 3492, 3503, 3585, 4364], 'ISG15.': [1742, 3618, 4013], 'Therefore,': [1743, 3040, 4149], 'Significantly,': [1751], 'vivomouse': [1753], 'data': [1754], 'demonstrates': [1756], 'crucial': [1770], 'proper': [1772], 'pcDNA6-UBP43': [1778, 2615], 'construct': [1779, 1884, 2079], 'UBP43-V5–6His': [1782, 2686], 'mammalian': [1785, 4164], 'described': [1790, 2032, 2180, 2355, 2823], '(25Schwer': [1791], 'Plasmids': [1814], 'expressing': [1815, 1963, 1996, 2080, 2119, 2220, 2261, 4234], 'UBP41': [1819], '(as': [1820], 'GST': [1821, 2335, 3406], 'fusion),': [1822], 'UBP1': [1823], '(33Tobias': [1824], 'J.W.': [1825], 'Varshavsky': [1826], '12021-12028Abstract': [1833], 'Unp,': [1840], 'Unp(mut)': [1842], '(34Gilchrist': [1843], 'C.A.': [1844], 'Biochim.': [1847], 'Acta.': [1849], '1481:': [1851], '297-309Crossref': [1852], '(22)': [1855], 'pUb-GSTP1': [1861, 2153], '(35Baker': [1862], 'Smith': [1864], 'S.A.': [1865], 'Marano': [1866], 'McKee': [1868], 'Board': [1870], 'P.G.': [1871], '269:': [1876], '25381-25386Abstract': [1877], 'provided': [1886, 3335], 'Dr.': [1888, 1901, 1948, 3337], '(Australian': [1891], 'National': [1892], 'University,': [1893], 'Canberra).': [1894], 'pGEX-Nedd8-gsPESTc': [1895, 1970, 2005], 'pGEX-SUMO-gsPESTc': [1897], 'K.': [1902, 1929, 2376, 2863, 3643, 3704, 4067], 'Tanaka': [1903, 1928, 2375, 2862, 3642, 3703, 4066], '(The': [1904], 'Tokyo': [1905], 'Metropolitan': [1906], 'Institute': [1907, 2953], 'Medical': [1909], 'Science,': [1910], 'Japan)': [1911], '(36Kawakami': [1912, 3687], 'Suzuki': [1914, 2361, 3689], 'Chung': [1918, 2365, 2864, 3644, 3693, 4068], 'Kawasaki': [1920, 2367, 3695], 'Hirano': [1922, 2369, 3697], 'Ichiyama': [1924, 2371, 3699], 'Omata': [1926, 2373, 3701], '(Tokyo).': [1932, 2379, 3707], '126:': [1934, 2381, 3709], '612-623Crossref': [1935, 2382, 3710], '(16)': [1938, 2385, 3713], 'pGEX-ISG15-Rcap': [1941, 1989, 2155], 'pRSV-ISG17': [1943], 'received': [1946], '(Medical': [1952], 'Colledge': [1953], 'Wisconsin,': [1955], 'Milwaukee,': [1956], 'WI).': [1957, 2075], 'To': [1958, 1992, 2076, 2216, 2882, 2963, 3716, 3866, 3955], 'produce': [1959, 1993], 'pGEX-ISG15-gsPESTc': [1960], 'pGEX-ISG17-gsPESTc': [1962], 'GST-ISG15-gsPESTc': [1964, 2311, 2324], 'GST-pro-ISG15-gsPESTc,': [1966], 'respectively,': [1967, 3175], 'plasmid': [1969, 1995, 2014, 2178, 2466], 'digested': [1972, 2085, 2110, 2286, 2326, 3893], 'withBamHI': [1973], 'excised': [1976, 2003, 2245], 'replaced': [1979, 2240], 'withISG15': [1980], 'ISG17': [1982], 'had': [1984, 2022, 2148, 3673], 'PCR': [1986, 2150], 'amplified': [1987, 2151], 'pRSV-ISG17.': [1991], 'pET-Ub-gsPESTc': [1994], 'Ub-gsPESTc': [1997, 2342, 4197], 'gsPESTc-coding': [2000], 'using': [2006, 2208, 2399, 2617, 3218, 3553, 3595], 'BamHI': [2007], 'EcoRI': [2009], 'pET-Ub-UBP43-H': [2013, 2115, 2979], '(see': [2015], 'below)': [2016], 'UBP43-H': [2019, 3888, 3898, 3998], 'encoding': [2020], 'excised.': [2024], 'Construction': [2025], 'pGEX-UBP43': [2027], '(pGEX-4T-3-UBP43)': [2028], 'pET-S-UBP43-H': [2060, 2083, 2136], 'produced': [2062, 2129, 2436, 3400, 3669], 'cloning': [2064], 'full-length': [2067, 2441, 3038], 'pET29a': [2071], 'vector': [2072], '(Novagen,': [2073], 'Madison,': [2074], 'generate': [2077, 4272], 'GST-UBP43–6His': [2081, 2449, 2530], 'withSacI': [2086], 'SmaI': [2088], 'restriction': [2089], 'endonucleases': [2090], 'excise': [2092], 'part': [2093, 2134, 3104, 3392], 'together': [2096], 'fused': [2099, 4365], 'His6': [2100], 'tag.': [2101], 'resulting': [2103, 3455], 'SacI-SmaI': [2104], 'fragment': [2105], 'pGEX-UBP43(mut)': [2109], 'SacI': [2112], 'andNotI': [2113], '(blunted).': [2114], 'pET-ISG15-UBP43-H': [2117, 2981], 'constructs': [2118, 2219, 2238, 2249, 2263, 4235], 'replacing': [2131], 'S-tag': [2133], '(digested': [2137], 'NdeI': [2139], 'andEcoR': [2140], 'V)': [2141], 'plasmids.': [2156, 4263], 'Inactive': [2157], 'form': [2158], 'obtained': [2162, 4099], 'conversion': [2164], 'cysteine': [2168, 3852], 'residue': [2169, 3853], '(Cys61)': [2170], 'serine': [2172], 'site-directed': [2174], 'mutagenesis': [2175], 'pBK/CMV-UBP43': [2177], '(previously': [2179], 'QuikChange': [2210], 'kit': [2211], '(Stratagene,': [2212], 'La': [2213], 'Jolla,': [2214], 'CA).': [2215, 2541, 2962], 'convert': [2217], 'wild-type': [2222, 2452, 2792, 2983, 3113, 3879, 3885, 3997, 4358], 'version': [2223, 3846, 3886], 'inactive': [2227, 2985, 3862], 'forms,': [2228], 'internal': [2229], 'SacI-XbaI': [2230], 'fragments': [2231, 2242], 'theUBP43': [2233], 'respective': [2237, 3489], 'withSacI-XbaI': [2241], 'UBP43(mut)': [2244], 'pBK-CMV-UBP43(mut).': [2247], 'All': [2248, 2591, 3478], 'generated': [2250], 'course': [2253], 'sequenced.': [2258], 'Ubl-gsPESTc': [2262, 2389, 3751], 'transformed': [2265, 2463, 2977], 'BL21(DE3)': [2267], 'coli': [2269, 2429, 2461, 2975, 2993, 3019, 3045, 3403, 3552, 3672, 4299, 4334], 'isopropyl-1-thio-β-d-galactopyranoside.': [2276, 2482], 'GST-Nedd8-gsPESTc': [2277], 'GST-SUMO-gsPESTc': [2279], 'GSH-agarose': [2284, 2298, 2316, 3419], '(Sigma),': [2285], 'thrombin': [2288, 2328], '(Amersham': [2290, 3437, 3497], 'Biosciences,': [2291, 3438], 'Inc.,': [2292], 'Piscataway,': [2293], 'NJ)': [2294], 'while': [2295, 3896, 4330], 'attached': [2296, 3417], 'beads': [2299], 'supernatants': [2302, 3089, 3198], 'containing': [2303, 2333, 2546, 2724], 'these': [2304, 4175], 'Ubls-gsPESTc': [2305], 'directly': [2307], 'labeling.': [2310, 2341], 'eluted': [2318, 2920], 'reduced': [2320], 'glutathion.': [2321], 'After': [2322, 2483, 2542], 'dialysis': [2323], 'solution': [2332], 'ISG15-gsPESTc': [2337, 3821, 3832, 3894, 4133, 4180], 'polypeptides': [2338], 'heat': [2347], 'denaturation,': [2348], '(NH4)2SO4': [2349], 'precipitation': [2350, 4024], 'ion-exchange': [2352], 'chromatography': [2353], 'Kawakami': [2357], 'al.(36Kawakami': [2359], 'radiolabeled': [2392], 'Na125I': [2394], '(ICN,': [2395], 'Costa': [2396], 'Mesa,': [2397], 'CA)': [2398, 3470], 'IODO-BEADS': [2400], '(Pierce,': [2401], 'Rockford,': [2402], 'IL)': [2403], '5': [2405, 2748, 2751, 3050, 3087, 3195, 3223, 3282, 3288], 'min': [2406, 2524, 3196, 3253, 3311], 'accordance': [2408], "manufacturer's": [2411, 2624], 'instructions.': [2412, 2625], 'labeling': [2414, 2421], 'conditions': [2415, 3737], 'resulted': [2417, 3001, 3031, 3999], 'almost': [2419], 'exclusive': [2420], 'gsPESTc': [2423, 3746, 3761, 3772, 3827, 4008], 'extension.': [2424, 3747], 'Expression': [2425], 'inE.': [2428], 'either': [2431, 3878, 4357], 'GST-UBP43': [2432, 3473], 'UBP43–6His': [2434], 'less': [2437], '30%': [2439, 2582], 'Therefore': [2443], 'UBP43gene': [2445], 'expressed': [2447, 4250, 4259, 4331, 4345], '(both': [2451], 'mutant': [2454, 2794, 3845, 3881], 'versions).': [2455], 'One': [2456], 'liter': [2457], 'BL21': [2459], 'culture': [2462, 2610], 'pGEX-UBP43-H': [2465], 'grown': [2468, 2994, 3048], 'LB': [2470], 'broth': [2471], 'toA': [2472], '600': [2473, 3058], '=': [2474, 3059], '0.8': [2475, 3060], '3': [2484, 2535], 'h': [2485, 3067], 'induction': [2487, 3025], 'at': [2488, 2526, 2586, 2596, 2681, 3028, 3068, 3083, 3191, 3201, 3254, 3313, 3324, 3347, 3384, 3422, 3521, 3963, 4124], '21': [2489, 3069], '°C,': [2490], 'harvested': [2493, 2636], 'centrifugation,': [2495], 'washed': [2496, 2569, 2632, 2700, 2908, 3118, 3259], 'PBS,': [2498, 2571], 'lysed': [2500, 2667, 3074], 'sonication': [2502, 3139], 'PBS': [2504, 2545, 2634, 2706, 3121], 'adjusted': [2505, 2644, 2707], '10': [2507, 2523, 2577, 2646, 2662, 2743, 2922, 3183, 3243, 3252, 3278, 3310], 'mm': [2508, 2511, 2516, 2548, 2551, 2578, 2647, 2653, 2656, 2710, 2713, 2744, 2749, 2752, 2755, 3148, 3153, 3227, 3232, 3237, 3283, 3286, 3289, 3292, 3511, 3519], 'imidazole,': [2509, 2552, 2648, 2714, 2756], '300': [2510, 2547, 2652, 2709], 'NaCl,': [2512, 2549, 2654, 2711, 3287], '2': [2513, 2515, 2599, 2655, 2658, 3152, 3173, 3231, 3236, 3239, 3291, 4205], 'mmβ-mercaptoethanol,': [2514], 'phenylmethylsulfonyl': [2517, 3154], 'fluoride.': [2518], 'Lysate': [2519, 2674], 'cleared': [2521, 2676], 'centrifugation': [2525, 2680, 3190, 3250, 3312], '20,000': [2527], '×g': [2528, 3193, 3256], 'absorbed': [2533, 2560, 2689], 'ml': [2536, 2563, 2639, 2704, 3051, 3145, 3224, 3263, 3276], 'Ni-NTA-agarose': [2538, 2694], '(Qiagen,': [2539], 'Valencia,': [2540], 'elution': [2543], '250': [2550], '0.1%': [2554, 2572, 2649, 2716, 2758, 2925], 'n-octyl': [2555, 2573, 2650, 2717, 2759, 3296], 'β-d-glucopyranoside,': [2556, 2574, 2651, 2718], '0.6': [2562], 'GSH-agarose.': [2565, 3411], 'resin': [2567, 3467], 'resuspended': [2575, 2738, 3273], 'Tris,': [2579, 2745, 3149, 3228, 3512], 'pH': [2580, 2746, 3150, 3229, 3280, 3513], '7.5,': [2581, 2747, 3151, 3230], 'glycerol': [2583], 'stored': [2585, 3200, 3323], '−20': [2587], '°C': [2588, 3203, 3523], 'until': [2589, 3204], 'use.': [2590, 3205], 'purification': [2592, 2790, 3461], 'procedures': [2593, 3480], 'performed': [2595, 2942, 3421, 3482], '+4': [2597], '°C.': [2598, 3070, 3326], '×': [2600, 2683, 3085, 3315], '106': [2601], '293T': [2603, 3875], 'seeded': [2606], '60-mm': [2608], 'plates': [2611], 'transfected': [2613, 3874], 'Polyfect': [2618], 'reagent': [2619], '(Qiagen)': [2620], 'according': [2621, 3483], 'Thirty-six': [2626], 'hours': [2627], 'transfection': [2629], '1': [2638, 2696, 2703, 2768, 3144, 3275, 3842, 3976, 4106], 'lysis': [2641], 'buffer': [2642, 2723, 2732, 2884], '(PBS': [2643], 'β-mercaptoethanol,': [2657, 3290], 'mmphenylmethylsulfonyl': [2659, 3240], 'fluoride,': [2660, 3155, 3241], 'μg/ml': [2663, 3244, 3353], 'leupeptin)': [2664], 'then': [2666, 3097, 3272, 3301], '5-s': [2670], 'pulse': [2671], 'sonication.': [2673], '5-min': [2679], '16,000': [2682, 3255], 'g': [2684], '40': [2691], 'μl': [2692, 2741, 2764, 2923], 'h.': [2697], 'Beads': [2698, 2736], 'once': [2701], '20': [2712, 2754, 3147, 3226], 'twice': [2719, 2729, 3260], 'same': [2722, 3266, 3965], '4': [2725, 3066], 'm': [2726], 'urea,': [2727], 'no': [2734, 4216, 4352], 'urea.': [2735], '80': [2740], 'EDTA,': [2750, 3284], 'dithiothreitol,': [2753], 'β-d-glucopyranoside.': [2760, 3297], 'suspensions': [2762, 3177], '(15': [2763], 'per': [2765, 4310], 'enzymatic': [2766, 2783], 'reaction,': [2767], 'μg': [2769, 2775], 'total': [2771, 3129, 3448], 'protein,': [2772], '0.1': [2774], 'fusion)': [2778], 'immediately': [2780, 3939], 'assay.': [2784, 4172], 'Equal': [2785], 'efficiency': [2786], 'expression,': [2788], 'between': [2791, 4420], 'UBP43s': [2795], 'confirmed': [2797], 'Western': [2799], 'blot': [2800, 3508], 'anti-V5': [2802], '(Invitrogen).': [2804], 'peptide': [2806, 2893, 2918, 4009, 4016, 4118], 'GST-proISG15-gsPESTc': [2809], 'recovered': [2815, 4018], 'trichloroacetic': [2818, 4026], 'acid-soluble': [2819], 'fraction': [2820, 3450, 3457], '37Woo': [2848, 4052], 'S.K.': [2849, 3629, 4053], 'Lee': [2850, 3630, 4054], 'J.I.': [2851, 3631, 4055], 'Park': [2852, 3632, 4056], 'I.K.': [2853, 3633, 4057], 'Yoo': [2854, 3634, 4058], 'Y.J.': [2855, 3635, 4059], 'Cho': [2856, 3636, 4060], 'Ha': [2860, 3640, 4064], 'D.B.': [2861, 3641, 4065], '1995;': [2869, 3649, 4073], '270:': [2870, 3650, 4074], '18766-18773Abstract': [2871, 3651, 4075], '(32)': [2879, 3659, 4083], 'components,': [2885], 'interfere': [2887], 'mass': [2889, 3778, 4090, 4098, 4112], 'spectroscopic': [2890], 'analysis,': [2891], 'C18-reverse': [2897], 'phase': [2898], 'matrix': [2899], '(Zip-Tip;': [2900], 'Millipore,': [2901], 'Bedford,': [2902], 'MA).': [2903, 3427, 3535], 'Matrix': [2904], 'equilibrated': [2906], '1%': [2910, 3171], '(v/v)': [2911, 2926, 2930], 'acetic': [2912, 2927], 'water': [2915], 'acid,': [2928], '75%': [2929], 'acetonitrile': [2931], 'water.': [2933], 'Linear': [2934], 'desorption/ionization': [2937], '(MALDI': [2938], 'TOF)-mass': [2939], 'spectrometry': [2940], 'Mass': [2945], 'Spectrometry': [2946], 'Core': [2947], 'Facility': [2948], 'Scripps': [2951], 'Research': [2952], 'Voyager-DE': [2955], 'station': [2957], '(PerSeptive': [2958], 'Biosystems,': [2959], 'Foster': [2960], 'City,': [2961], 'assess': [2964], 'Ub-UBP43': [2968, 4319], 'undergo': [2973], 'self-hydrolysis,E.': [2974], 'carrying': [2982], 'versions': [2986, 4361], 'ofUBP43.': [2987], 'Production': [2988], 'rich': [2996], 'media,': [2997], 'LB,': [3000], 'high': [3003], 'yield': [3004], '(more': [3007], '70%)': [3009], 'On': [3013], 'contrary,': [3015], 'growth': [3016], 'nutrient-poor': [3021], 'medium': [3022, 3055], 'M9': [3023, 3053], 'low': [3029, 3776], 'temperature,': [3030], 'production': [3033], 'small': [3035], 'quantities': [3036], 'experiment,': [3043], 'cultures': [3046], 'minimal': [3054], 'A': [3057, 3435, 3619], 'isopropyl-1-thio-β-d-galactopyranoside': [3064], 'Cells': [3071], 'harvested,': [3073], 'sonication,': [3076], 'removal': [3079, 4001], 'insoluble': [3081, 3306], 'material': [3082, 3186, 3307], '18,000': [3084, 3192], 'gfor': [3086], 'min,': [3088], 'resolved': [3091], '10%': [3093], 'SDS-PAGE,': [3094], 'electroblotted,': [3095], 'probed': [3098], 'NH2-terminal': [3103, 3391], 'Lungs': [3107], 'surgically': [3109], 'removed': [3110, 3188, 3308, 3444], 'euthanized': [3112], 'UBP43−/−mice': [3115], '(3.5-week': [3116], 'old)': [3117], 'cold': [3120], 'kept': [3123], 'ice.': [3125], 'For': [3126, 3206, 3387], 'preparation': [3127, 3207], 'extracts': [3131], 'pair': [3133], 'lungs': [3135, 3211], 'homogenized': [3137, 3217], '(four': [3140], '8-s': [3141], 'pulses)': [3142], 'inhibitor': [3158], 'mixture': [3159], '(Sigma,': [3160], 'product': [3161], 'P2714).': [3163], 'Triton': [3164], 'X-100': [3165], 'β-mercaptoethanol': [3167], 'added': [3169], 'mm,': [3174], 'incubated': [3179], 'ice': [3181], 'min.': [3184, 3526], 'Particulate': [3185, 3246], '−80': [3202, 3325], 'cytoskeleton-enriched': [3209], 'fraction,': [3210], 'combined': [3212], 'mice': [3215], 'tissue': [3220], 'homogenizer': [3221], 'EGTA,': [3233], 'mmNaCl,': [3235], 'MgCl2,': [3238], 'leupeptin.': [3245], 'collected': [3248], 'thoroughly': [3258], '7': [3262], 'buffer.': [3267], 'final': [3269, 3349], 'pellet': [3270], 'mmTris,': [3279], '8.0,': [3281], 'CaCl2,': [3293], '0.5%': [3295], 'suspension': [3299], 'briefly': [3302], 'sonicated': [3303], 'any': [3305, 3864], '15,000': [3314], 'g.': [3316], 'Supernatant': [3317], 'designated': [3318], '“cytoskeleton-enriched': [3320], 'fraction”': [3321], 'Rabbit': [3327, 3374], 'polyclonal': [3328], 'IgGs': [3329, 3429], 'kindly': [3334], '(Cleveland': [3340], 'Clinic': [3341], 'Foundation,': [3342], 'OH)': [3343], 'concentration': [3350], '0.5': [3352], "(11D'Cunha": [3354], 'anti-serum': [3375], 'purchased': [3379], 'Sigma': [3381], '1:300': [3385], 'dilution.': [3386], 'anti-UBP43': [3388], '(amino': [3393], 'acids': [3394], '1–120)': [3395], 'murine': [3397], 'Immunization': [3412], 'rabbits': [3414], 'GST-UBP43(1–120)': [3416], 'Charles': [3423], 'River': [3424], 'Pharmservices': [3425], '(Southbridge,': [3426], 'Total': [3428], 'column': [3436], 'Inc.)': [3439], 'GST-specific': [3441], 'passing': [3446], 'IgG': [3449, 3456], 'through': [3451], 'GST-agarose': [3452], '(Pierce).': [3453], 'subjected': [3459], 'UBP43-specific': [3463], 'Affi-Gel': [3466], '(Bio-Rad,': [3468], 'Hercules,': [3469], 'coupled.': [3477], 'chromatographic': [3479], 'instructions': [3486], 'manufacturers.': [3490], 'Proteins': [3491], 'electroblotted': [3493], 'onto': [3494], 'nitrocellulose': [3495], 'membranes': [3496, 3502], 'Biosciences': [3498], 'Inc.).': [3499], 'Where': [3500], 'indicated,': [3501], 'stripped': [3504], '50': [3510, 3518], '7.0,': [3514], '2%': [3515], 'SDS,': [3516], 'dithiothreitol': [3520], '55': [3522], '30': [3525], 'Chemiluminescence': [3527], 'PerkinElmer': [3531], 'Life': [3532], 'Sciences': [3533], '(Boston,': [3534], 'detection': [3547], 'able': [3587, 4405, 4411], 'variety': [3597], 'fusions.': [3600, 4322], 'This': [3601], 'fact': [3602], 'prompted': [3603], 'analyze': [3606], 'Ubls,': [3613], 'namely': [3614], 'sensitive': [3620], 'developed': [3623], 'Woo': [3625], '(37Woo': [3628], 'selected': [3663], 'purpose.': [3666], 'Each': [3667], 'Ubl': [3668], 'cleavable': [3675], 'carboxyl-terminal': [3676, 3950, 4004], 'extension': [3677, 3762, 4006, 4377], 'following': [3680, 4128], 'GSMHISPPEPESEEEEEHYC': [3682], '(referred': [3683], 'gsPESTc)': [3686], 'avoid': [3717], 'covalent': [3718], 'modifications': [3719], 'backbones': [3722], 'caused': [3725], '125I': [3727, 3740], 'attachment': [3728], 'artifacts': [3731, 4156], 'under': [3738], 'exclusively': [3741], 'labels': [3742], 'tyrosine': [3744], 'As': [3748, 3815, 3984], 'consequence,': [3750], '(detectable': [3753], 'when': [3754, 4342, 4356, 4388], 'intact)': [3755], 'hydrolysis': [3757, 3958, 4338, 4353], 'loses': [3758], 'labeled': [3760], 'appears': [3764], 'unlabeled': [3766, 3982], 'autoradiograms.': [3771], 'due': [3773], 'molecular': [3777, 4089, 4097], 'could': [3779, 4178, 4182, 4326, 4339], 'detected': [3781], 'Tris-Tricine': [3783], '(38Schagger': [3784], 'von': [3786], 'Jagow': [3787], 'Anal.': [3789], '166:': [3792], '368-379Crossref': [3793], '(10505)': [3796], 'regular': [3802], 'SDS-PAGE': [3803], '(39Laemmli': [3804], 'U.K.': [3805], '1970;': [3807], '227:': [3808], '680-685Crossref': [3809], '(207538)': [3812], 'evidenced': [3816, 3985], 'disappearance': [3818], 'band': [3822, 3987], 'appearance': [3824], 'band,': [3828], 'hydrolyzed': [3830, 4196, 4329], 'Ub-,': [3836], 'Nedd8-,': [3837], 'SUMO-gsPESTc': [3839], '(Fig.': [3841, 4335], 'A).': [3843], 'converted': [3858], 'serine,': [3860], 'substrate.': [3865], 'confirm': [3867, 3956], 'observation': [3869], 'mammalian-expressed': [3871], 'His6-tagged': [3882], 'Ni-agarose': [3891], 'mutated': [3897, 4360], '(Fig.1': [3901, 3989], 'B).': [3902], 'Members': [3903], 'pro-ISG15': [3908], 'processing': [3909, 3969], 'perform': [3935], 'cleavage': [3936], 'LRLRGG': [3941], 'motif': [3942], '(identical': [3943], 'ISG15)': [3947], 'yielding': [3948], 'diglycine': [3951], 'available': [3952], 'catalyzed': [3959], 'site,': [3966], 'larger': [3968], 'reaction': [3970, 4021], 'Fig.': [3975, 4105], 'carried': [3979], 'out': [3980], 'GST-pro-ISG15-gsPESTc.': [3983], 'shift': [3988], 'C,': [3990], 'inset),': [3991], 'incubation': [3993, 4396], 'GST-pro-ISG15-gsPESTc': [3995], 'octapeptide': [4005], '(GTEPGGRSGSMHISPPEPESEEEEEHYC)': [4010], 'leaving': [4011], 'mature': [4012], 'selective': [4023], 'exact': [4088], 'determined': [4092], 'MALDI': [4094], 'analysis.': [4095], 'experiment': [4102], '(3059': [4103], 'Da;': [4104], 'C)': [4107], 'corresponded': [4108], 'predicted': [4111], '(3058.17': [4113], 'Da)': [4114], 'suggesting': [4119], 'expected': [4126], 'site': [4127], 'diglycine.': [4129], 'use': [4131], 'never': [4136], 'before': [4139], 'ISG15-gsPESTc.': [4148], 'eliminate': [4151], 'tested': [4158], 'yeast': [4160], '(UBP1)': [4161], '(UBP41': [4166], 'Unp)': [4168], 'identical': [4171], 'None': [4173], '(nor': [4181], 'they': [4183, 4290], 'Nedd8-': [4185], 'SUMO-gsPESTc,': [4187], 'shown)': [4189], 'while,': [4190], 'expected,': [4192], '(Fig.2).': [4198], 'results': [4200], 'presented': [4201, 4240], 'Figs.1': [4203], 'suggest': [4207], 'exhibits': [4215], 'Ubls.': [4221], 'Detection': [4222], 'colico-transformed': [4229], 'contradicted': [4236], 'Fig.1.': [4242], 'assay,': [4247], 'low-copy': [4252], 'pACYC': [4254], 'plasmid,': [4255], 'whereas': [4256], 'high-copy': [4261], 'Such': [4264], 'difference': [4266], 'likely': [4270], 'excess': [4274, 4390], 'over': [4277], 'substrate': [4279], 'permit': [4282], 'even': [4288, 4387], 'though': [4289], 'redesigned': [4297], 'theE.': [4298], 'allow': [4302], 'copy': [4304, 4312], 'synthesized': [4309], 'making': [4318], 'Only': [4323], 'ISG15-UBP43(wt)': [4324], '3).': [4336], 'observed': [4341, 4355], 'ISG15-UBP43(mut)': [4343], 'consistent': [4347], 'experiments,': [4351], 'Ub.': [4367], 'Use': [4368], '(Ub-carboxyl-terminal': [4376], 'proteins)': [4378], 'technique,': [4385], 'yet,': [4386], 'times': [4397], 'extended,': [4399], 'bond,': [4416], 'i.e.': [4417], 'sid': [4425]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2147718057', 'counts_by_year': [{'year': 2024, 'cited_by_count': 26}, {'year': 2023, 'cited_by_count': 30}, {'year': 2022, 'cited_by_count': 29}, {'year': 2021, 'cited_by_count': 27}, {'year': 2020, 'cited_by_count': 30}, {'year': 2019, 'cited_by_count': 26}, {'year': 2018, 'cited_by_count': 17}, {'year': 2017, 'cited_by_count': 20}, {'year': 2016, 'cited_by_count': 17}, {'year': 2015, 'cited_by_count': 21}, {'year': 2014, 'cited_by_count': 24}, {'year': 2013, 'cited_by_count': 25}, {'year': 2012, 'cited_by_count': 15}], 'updated_date': '2024-12-31T05:00:12.658108', 'created_date': '2016-06-24'}