Title: Characterisation and genetic diversity of pepper leafroll virus, a new bipartite begomovirus infecting pepper, bean and tomato in Peru
Abstract: Annals of Applied BiologyVolume 164, Issue 1 p. 62-72 RESEARCH ARTICLE Characterisation and genetic diversity of pepper leafroll virus, a new bipartite begomovirus infecting pepper, bean and tomato in Peru A. Martínez-Ayala, A. Martínez-Ayala Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora' (IHSM-UMA-CSIC), Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Estación Experimental 'La Mayora', 29750 Algarrobo-Costa, Málaga, SpainThese authors contributed equally to this workSearch for more papers by this authorS. Sánchez-Campos, S. Sánchez-Campos Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora' (IHSM-UMA-CSIC), Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Estación Experimental 'La Mayora', 29750 Algarrobo-Costa, Málaga, SpainThese authors contributed equally to this workSearch for more papers by this authorF. Cáceres, F. Cáceres Facultad de Ciencias Biológicas y Agropecuarias, Universidad Nacional de San Agustín, Arequipa, PeruSearch for more papers by this authorL. Aragón-Caballero, L. Aragón-Caballero Facultad de Agronomía, Universidad Nacional Agraria La Molina, Av. La Molina s/n, La Molina, Lima, PeruSearch for more papers by this authorJ. Navas-Castillo, J. Navas-Castillo Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora' (IHSM-UMA-CSIC), Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Estación Experimental 'La Mayora', 29750 Algarrobo-Costa, Málaga, SpainSearch for more papers by this authorE. Moriones, E. Moriones Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora' (IHSM-UMA-CSIC), Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Estación Experimental 'La Mayora', 29750 Algarrobo-Costa, Málaga, SpainSearch for more papers by this author A. Martínez-Ayala, A. Martínez-Ayala Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora' (IHSM-UMA-CSIC), Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Estación Experimental 'La Mayora', 29750 Algarrobo-Costa, Málaga, SpainThese authors contributed equally to this workSearch for more papers by this authorS. Sánchez-Campos, S. Sánchez-Campos Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora' (IHSM-UMA-CSIC), Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Estación Experimental 'La Mayora', 29750 Algarrobo-Costa, Málaga, SpainThese authors contributed equally to this workSearch for more papers by this authorF. Cáceres, F. Cáceres Facultad de Ciencias Biológicas y Agropecuarias, Universidad Nacional de San Agustín, Arequipa, PeruSearch for more papers by this authorL. Aragón-Caballero, L. Aragón-Caballero Facultad de Agronomía, Universidad Nacional Agraria La Molina, Av. La Molina s/n, La Molina, Lima, PeruSearch for more papers by this authorJ. Navas-Castillo, J. Navas-Castillo Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora' (IHSM-UMA-CSIC), Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Estación Experimental 'La Mayora', 29750 Algarrobo-Costa, Málaga, SpainSearch for more papers by this authorE. Moriones, E. Moriones Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora' (IHSM-UMA-CSIC), Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Estación Experimental 'La Mayora', 29750 Algarrobo-Costa, Málaga, SpainSearch for more papers by this author First published: 03 October 2013 https://doi.org/10.1111/aab.12074Citations: 20 Correspondence E. Moriones, Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora' (IHSM-UMA-CSIC), Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Estación Experimental 'La Mayora', 29750 Algarrobo-Costa, Málaga, Spain. Email: [email protected] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The complete genome of a novel bipartite begomovirus (genus Begomovirus, family Geminiviridae) was cloned from a severely diseased yellow Peruvian chili pepper (Capsicum baccatum cv. Pendulum) plant collected in the department of La Libertad, Northern Peru and full-length sequenced. The two genomic components share a common region of 156 nucleotides with a 100% sequence identity. Analysis of the genome organisation and phylogenetic comparisons revealed that the virus is a typical New World begomovirus. The closest related begomovirus, an isolate of Tomato yellow vein streak virus (ToYVSV), shared only 76.8% nucleotide sequence identity for the DNA-A component. Therefore, following species demarcation criteria of the International Committee on Taxonomy of Viruses, this virus isolate belongs to a new begomovirus species for which the name pepper leafroll virus (PepLRV) is proposed. Pepper plants infected with the cloned PepLRV isolate developed leaf roll symptoms similar to those observed in field-infected plants suggesting this virus as the causal agent of the disease syndrome observed in the field. Widespread occurrence of PepLRV throughout Peru was demonstrated, infecting plants of diverse cultivated species such as tomato, pepper, common and pallar beans, and of the weed species Nicandra physaloides. Low genetic diversity was observed among PepLRV isolates present in this country with no evident geographical or temporal structure of the population, typical of a recent founder effect. This is the first report of a begomovirus infecting pepper and bean crops in Peru. Supporting Information Filename Description aab12074-sup-0001-FigureS1.tifTIFF image, 78.1 KB Fig. S1. Schematic representation of the genomic organisation of pepper leafroll virus (PepLRV) showing the two single stranded circular DNA genomic components (DNA-A and DNA-B) of ˜2.6 kb in length. Arrows indicate the open reading frames encoding the coat protein (CP), the replication-associated protein (Rep), protein C4, the transcriptional activator protein (TrAP), and the replication enhancer protein (REn) in DNA-A and the nuclear shuttle protein (NSP) and the movement protein (MP) in DNA-B; in both DNAs, the intergenic region (IR) containing the stem-loop structure, and the common region (CR) between the two components (grey box) are shown. Also, the 992-nt fragment of the DNA-A component considered for the genetic diversity study of PepLRV isolates is shown (black box). aab12074-sup-0002-FigureS2.tifTIFF image, 94.2 KB Fig. S2. A. Colour representation of the pairwise nucleotide sequence identity percentages calculated between a 992-nt fragment [nt 1807 to 230 of the DNA-A component (numbers based on the sequence of the PepLRV isolate PE:P107:pep:2009, GenBank accession number KC769819)] of pepper leafroll virus (PepLRV) isolates collected in a surveys conducted in Peru during 2009 and 2010. The equivalent sequence for the DNA component of the begomovirus tomato leaf deformation virus ToLDeV (GenBank accession number GQ334472) was also included in the comparison. MEGA 5.0 programme (Tamura et al., 2011) was used to calculate the percentages of nucleotide sequence identity by the Kimura 2-parameter distance model. The lowest identity percentage value is underlined. The corresponding phylogenetic tree constructed using the Maximum Likelihood method is included (B). Bootstrap (1000 replicates) values are expressed in percentages, and only the nodes with values greater than 50% are labelled. 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Publication Year: 2013
Publication Date: 2013-10-03
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 24
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