Title: Distinct glycan‐charged phosphodolichol carriers are required for the assembly of the pentasaccharide N‐linked to the <i>Haloferax volcanii</i> S‐layer glycoprotein
Abstract: Summary In Archaea, dolichol phosphates have been implicated as glycan carriers in the N‐glycosylation pathway, much like their eukaryal counterparts. To clarify this relation, highly sensitive liquid chromatography/mass spectrometry was employed to detect and characterize glycan‐charged phosphodolichols in the haloarchaeon Haloferax volcanii . It is reported that Hfx. volcanii contains a series of C 55 and C 60 dolichol phosphates presenting saturated isoprene subunits at the α and ω positions and sequentially modified with the first, second, third and methylated fourth sugar subunits comprising the first four subunits of the pentasaccharide N‐linked to the S‐layer glycoprotein, a reporter of N‐glycosylation. Moreover, when this glycan‐charged phosphodolichol pool was examined in cells deleted of agl genes encoding glycosyltransferases participating in N‐glycosylation and previously assigned roles in adding pentasaccharide residues one to four, the composition of the lipid‐linked glycans was perturbed in the identical manner as was S‐layer glycoprotein N‐glycosylation in these mutants. In contrast, the fifth sugar of the pentasaccharide, identified as mannose in this study, is added to a distinct dolichol phosphate carrier. This represents the first evidence that in Archaea, as in Eukarya, the oligosaccharides N‐linked to glycoproteins are sequentially assembled from glycans originating from distinct phosphodolichol carriers.