Title: Analysis of protoberberine alkaloids in several herbal drugs and related medicinal preparations by non-aqueous capillary electrophoresis
Abstract: Journal of Separation ScienceVolume 28, Issue 1 p. 92-97 Short Communication Analysis of protoberberine alkaloids in several herbal drugs and related medicinal preparations by non-aqueous capillary electrophoresis Wen Hua Gao, Wen Hua Gao [email protected] Department of Chemistry, Lanzhou University, Lanzhou 730000, P.R. China. Phone: +86 931 8912540. Fax: +86 931 8912582 Department of Chemistry, Shantou University, Shantou 515063, P.R. ChinaSearch for more papers by this authorShao Yu Lin, Shao Yu Lin Longhu Subbureau, Shantou Environmental Protection Bureau, Shantou 515041, P.R. ChinaSearch for more papers by this authorLi Jia, Li Jia Department of Chemistry, Shantou University, Shantou 515063, P.R. ChinaSearch for more papers by this authorXi Kun Guo, Xi Kun Guo Department of Chemistry, Shantou University, Shantou 515063, P.R. ChinaSearch for more papers by this authorXing Guo Chen, Xing Guo Chen Department of Chemistry, Lanzhou University, Lanzhou 730000, P.R. China. Phone: +86 931 8912540. Fax: +86 931 8912582Search for more papers by this authorZhi De Hu, Zhi De Hu [email protected] Search for more papers by this author Wen Hua Gao, Wen Hua Gao [email protected] Department of Chemistry, Lanzhou University, Lanzhou 730000, P.R. China. Phone: +86 931 8912540. Fax: +86 931 8912582 Department of Chemistry, Shantou University, Shantou 515063, P.R. ChinaSearch for more papers by this authorShao Yu Lin, Shao Yu Lin Longhu Subbureau, Shantou Environmental Protection Bureau, Shantou 515041, P.R. ChinaSearch for more papers by this authorLi Jia, Li Jia Department of Chemistry, Shantou University, Shantou 515063, P.R. ChinaSearch for more papers by this authorXi Kun Guo, Xi Kun Guo Department of Chemistry, Shantou University, Shantou 515063, P.R. ChinaSearch for more papers by this authorXing Guo Chen, Xing Guo Chen Department of Chemistry, Lanzhou University, Lanzhou 730000, P.R. China. Phone: +86 931 8912540. Fax: +86 931 8912582Search for more papers by this authorZhi De Hu, Zhi De Hu [email protected] Search for more papers by this author First published: 27 December 2004 https://doi.org/10.1002/jssc.200401863Citations: 29AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract A simple, rapid, reproducible, and universal non-aqueous capillary electrophoresis method has been developed for the separation and determination of three major active protoberberine alkaloids including berberine, palmatine, and jatrorrhizine within 7 min. The effects of the concentrations of acetic acid and electrolyte, the ratio of organic solvent, and the applied voltage on the separation were investigated. The optimum running buffer was composed of 50 mM ammonium acetate, 0.5% (v/v) acetic acid, and 10% (v/v) acetonitrile in methanol. The applied voltage was 18 kV. The analytes were detected by UV at 214 nm. The linearities between peak areas and the concentrations of the analytes were also investigated, and they exhibit excellent linear behavior over the concentration ranges (correlation coefficients: 0.9975–0.9986). The method was successfully applied to determine the three alkaloids in several families of herbal drugs (Rhizoma Coptidis, Cortex Berberidis, Cortex Phellodendri, Herba Chelidonii, Caulis Mahoniae) and their relevant medicinal preparations for the first time, and the recoveries of the three constituents ranged between 95.6–103.2% for berberine, 97.5–103.3% for palmatine, and 96.1–103.6% for jatrorrhizine. Citing Literature Volume28, Issue1January 2005Pages 92-97 RelatedInformation
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 38
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