Title: Effect of long time storage on cytosolic glutathione S‐transferases
Abstract:Abstract Glutathione S‐transferases are a family of enzymes catalyzing the conjugation of glutathione to a wide variety of compounds. Stability of human liver cytosolic glutathione S‐transferase enzym...Abstract Glutathione S‐transferases are a family of enzymes catalyzing the conjugation of glutathione to a wide variety of compounds. Stability of human liver cytosolic glutathione S‐transferase enzyme activity and levels of glutathione S‐transferase class Alpha and Mu were assessed. Cytosolic fractions of sixteen different patients were stored up to two years at three different temperatures: ‐20°, ‐80° and ‐196° Celsius. Samples were assayed for glutathione S‐transferase enzyme activity and glutathione S‐transferase class Alpha and Mu levels, after 3, 6, 12 and 24 months of storage. No significant differences in glutathione S‐transferase enzyme activity as well as in isoenzyme levels were found during this 24 months of storage at the three different temperatures. It is concluded that stability of glutathione S‐transferases allows long term storage even at ‐20°C, without significant loss of enzyme activity or enzyme protein.Read More
Publication Year: 1997
Publication Date: 1997-04-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 4
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