Title: Comparative analysis of genetic diversity in Prunus L. as revealed by RAPD and SSR markers
Abstract: RAPD and SSR markers were used for genetic diversity evaluations among 15 genotypes selected from the genus Prunus L. Altogether 40 RAPD primers and 21 primer pairs designated for microsatellite loci were applied on the whole group of genotypes. The RAPD primers and SSR loci with the highest degree of observed polymorphism were selected. Three approaches were used to generate DNA-based genetic markers—RAPD, SSR with separations on PAGE, SSR with separations on MetaPhor agarose. An analysis of each approach was statistically summarized and the obtained parameters were compared. On the basis of the data obtained by individual methods, the relevant dendrograms of genetic similarities among genotypes were constructed. The Mantel tests between individual similarity matrices showed a statistically significant correlation between the RAPD- and SSR-based similarities if computed for the whole group of analyzed genotypes. Correlations hugely decrease in the case of a parallel analysis of a low-diversity group where Prunus persica L. cultivars have been selected only. Statistically significant correlations were computed between both approaches used for the separation of SSR products. The shapes of individual dendrograms were compared with theoretical expectations based on the botanical pedigree of the analyzed genotypes. The best correspondence with theoretical expectations is clearly shown by the dendrogram developed on the basis of RAPD results. Permutation analysis was used to evaluate the impact of the number of evaluated RAPD fragments on the final shape of the dendrogram. According to the observed characteristics of the individual methods, some general principles and recommendations for their future application were formulated.
Publication Year: 2006
Publication Date: 2006-03-30
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 28
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