Title: The Isolation and Characterization of the Two Macromolecular Antitumor Agents from Streptomyces
Abstract: Purification of the two antitumor macromolecules, A216 and A280 substances, from culture filtrates of Streptomyces is achieved by chromatography using ion-exchanged celluloses. The purified macromolecules appeared homogeneous are characterized as a protein from the chemical and biological properties by paper electrophoresis, paper chromatography and ultracentrifuge. The simple method for approximation of molecular weight of a protein from distribution coefficient on gel filtration is proposed. The molecular weights of both macromolecules given by gel filtration are near to those given by ultracentrifugal analysis.
Publication Year: 1962
Publication Date: 1962-09-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 2
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