Indirect Immunofluorescence of Tissues

Name: Work Video:
Duration: 3 min 30 s
Description: Abstract Indirect immunofluorescence is a two‐step procedure whereby unlabelled antibodies are first bound to their target antigen and subsequently detected with fluorochrome‐conjugated secondary antibodies (indirect immunofluorescence). This technique is widely used for histological analysis of tissues in basic and clinical research.
Wolfgang Härtig; Jean‐Marc Fritschy
{'id': 'https://openalex.org/W2133249119', 'doi': 'https://doi.org/10.1038/npg.els.0002628', 'title': 'Indirect Immunofluorescence of Tissues', 'display_name': 'Indirect Immunofluorescence of Tissues', 'publication_year': 2001, 'publication_date': '2001-04-19', 'ids': {'openalex': 'https://openalex.org/W2133249119', 'doi': 'https://doi.org/10.1038/npg.els.0002628', 'mag': '2133249119'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1038/npg.els.0002628', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4363604175', 'display_name': 'Encyclopedia of Life Sciences', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': None, 'host_organization_name': None, 'host_organization_lineage': [], 'host_organization_lineage_names': [], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'other', 'type_crossref': 'other', 'indexed_in': ['crossref'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5103847497', 'display_name': 'Wolfgang Härtig', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I926574661', 'display_name': 'Leipzig University', 'ror': 'https://ror.org/03s7gtk40', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I926574661']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Wolfgang Härtig', 'raw_affiliation_strings': ['University of Leipzig, Leipzig, Germany,'], 'affiliations': [{'raw_affiliation_string': 'University of Leipzig, Leipzig, Germany,', 'institution_ids': ['https://openalex.org/I926574661']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5052503226', 'display_name': 'Jean‐Marc Fritschy', 'orcid': 'https://orcid.org/0000-0002-8552-3702'}, 'institutions': [{'id': 'https://openalex.org/I202697423', 'display_name': 'University of Zurich', 'ror': 'https://ror.org/02crff812', 'country_code': 'CH', 'type': 'education', 'lineage': ['https://openalex.org/I202697423']}], 'countries': ['CH'], 'is_corresponding': False, 'raw_author_name': 'Jean‐Marc Fritschy', 'raw_affiliation_strings': ['[University of Zurich, Zürich, Switzerland]'], 'affiliations': [{'raw_affiliation_string': '[University of Zurich, Zürich, Switzerland]', 'institution_ids': ['https://openalex.org/I202697423']}]}], 'institution_assertions': [], 'countries_distinct_count': 2, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': None, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 4, 'citation_normalized_percentile': {'value': 0.820513, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 72, 'max': 74}, 'biblio': {'volume': None, 'issue': None, 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11016', 'display_name': 'Monoclonal and Polyclonal Antibodies Research', 'score': 0.9976, 'subfield': {'id': 'https://openalex.org/subfields/2741', 'display_name': 'Radiology, Nuclear Medicine and Imaging'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11016', 'display_name': 'Monoclonal and Polyclonal Antibodies Research', 'score': 0.9976, 'subfield': {'id': 'https://openalex.org/subfields/2741', 'display_name': 'Radiology, Nuclear Medicine and Imaging'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T10602', 'display_name': 'Glycosylation and Glycoproteins Research', 'score': 0.9936, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10981', 'display_name': 'Cytomegalovirus and herpesvirus research', 'score': 0.9789, 'subfield': {'id': 'https://openalex.org/subfields/2713', 'display_name': 'Epidemiology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/immunofluorescence', 'display_name': 'Immunofluorescence', 'score': 0.938747}, {'id': 'https://openalex.org/keywords/direct-fluorescent-antibody', 'display_name': 'Direct fluorescent antibody', 'score': 0.5244254}], 'concepts': [{'id': 'https://openalex.org/C2778236600', 'wikidata': 'https://www.wikidata.org/wiki/Q592324', 'display_name': 'Immunofluorescence', 'level': 3, 'score': 0.938747}, {'id': 'https://openalex.org/C2984894117', 'wikidata': 'https://www.wikidata.org/wiki/Q592324', 'display_name': 'Indirect immunofluorescence', 'level': 3, 'score': 0.81533706}, {'id': 'https://openalex.org/C159654299', 'wikidata': 'https://www.wikidata.org/wiki/Q79460', 'display_name': 'Antibody', 'level': 2, 'score': 0.63440704}, {'id': 'https://openalex.org/C98246554', 'wikidata': 'https://www.wikidata.org/wiki/Q5280334', 'display_name': 'Direct fluorescent antibody', 'level': 3, 'score': 0.5244254}, {'id': 'https://openalex.org/C147483822', 'wikidata': 'https://www.wikidata.org/wiki/Q103537', 'display_name': 'Antigen', 'level': 2, 'score': 0.49878}, {'id': 'https://openalex.org/C21269302', 'wikidata': 'https://www.wikidata.org/wiki/Q7243098', 'display_name': 'Primary and secondary antibodies', 'level': 3, 'score': 0.43809605}, {'id': 'https://openalex.org/C142724271', 'wikidata': 'https://www.wikidata.org/wiki/Q7208', 'display_name': 'Pathology', 'level': 1, 'score': 0.4301707}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.3541224}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.3477376}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.3238141}, {'id': 'https://openalex.org/C71924100', 'wikidata': 'https://www.wikidata.org/wiki/Q11190', 'display_name': 'Medicine', 'level': 0, 'score': 0.27743265}, {'id': 'https://openalex.org/C203014093', 'wikidata': 'https://www.wikidata.org/wiki/Q101929', 'display_name': 'Immunology', 'level': 1, 'score': 0.264086}], 'mesh': [], 'locations_count': 1, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1038/npg.els.0002628', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4363604175', 'display_name': 'Encyclopedia of Life Sciences', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': None, 'host_organization_name': None, 'host_organization_lineage': [], 'host_organization_lineage_names': [], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 11, 'referenced_works': ['https://openalex.org/W1535039738', 'https://openalex.org/W1603094194', 'https://openalex.org/W1984806793', 'https://openalex.org/W2003179114', 'https://openalex.org/W2035646534', 'https://openalex.org/W2083758505', 'https://openalex.org/W2095532144', 'https://openalex.org/W2149295320', 'https://openalex.org/W2150852780', 'https://openalex.org/W2171658838', 'https://openalex.org/W411779381'], 'related_works': ['https://openalex.org/W4200040722', 'https://openalex.org/W37027104', 'https://openalex.org/W2438102269', 'https://openalex.org/W2419025018', 'https://openalex.org/W2409095608', 'https://openalex.org/W2041385092', 'https://openalex.org/W2014636740', 'https://openalex.org/W1607889907', 'https://openalex.org/W1502680840', 'https://openalex.org/W111776578'], 'abstract_inverted_index': {'Abstract': [0], 'Indirect': [1], 'immunofluorescence': [2], 'is': [3, 28], 'a': [4], 'two‐step': [5], 'procedure': [6], 'whereby': [7], 'unlabelled': [8], 'antibodies': [9, 23], 'are': [10], 'first': [11], 'bound': [12], 'to': [13], 'their': [14], 'target': [15], 'antigen': [16], 'and': [17, 38], 'subsequently': [18], 'detected': [19], 'with': [20], 'fluorochrome‐conjugated': [21], 'secondary': [22], '(indirect': [24], 'immunofluorescence).': [25], 'This': [26], 'technique': [27], 'widely': [29], 'used': [30], 'for': [31], 'histological': [32], 'analysis': [33], 'of': [34], 'tissues': [35], 'in': [36], 'basic': [37], 'clinical': [39], 'research.': [40]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2133249119', 'counts_by_year': [{'year': 2017, 'cited_by_count': 1}, {'year': 2016, 'cited_by_count': 1}, {'year': 2015, 'cited_by_count': 2}], 'updated_date': '2024-12-10T15:59:27.336647', 'created_date': '2016-06-24'}
Publication Information

Basic Information

Access and Citation

AI Researcher Chatbot

Primary Location

Authors

Topics

Keywords

Related Works
