Title: Genetic variation among accessions of Festuca arundinacea Based on storage protein and soluble protein by SDS polyacrylamide gel electrophoresis
Abstract: The genetic variation of seed storage protein and leaf soluble protein was assayed by SDS-PAGE for 14 accessions of Festuca arundinacea. Protein fragments of various molecular weights were separated in Festuca accessions. On the basis of the relative mobility of seed proteins on the gel, 9 bands and on the basis of the relative mobility of leaf proteins on the gel, 12 bands were detected in this study. The banding pattern revealed three regions. The greatest protein diversity was observed in area of 42-70 kDa. The majority of the similarity coefficients for seed protein and leaf protein between accessions were close to 0.78-0.82. This indicated the close relationships between the evaluated accessions, though they are collected from different origin. seed storage protein and leaf soluble protein based on UPGMA hierarchical clustering for grouping accessions based on Dice's coefficient were identified the three distinctive groups. Scatter plot for accessions based on first and second axis from principal coordinate analysis showed that genetic variation did not matching with the geographical distribution of accessions.