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1274], '3,': [638, 1314], '473.': [639], '12S.': [640], 'Chan,': [642], 'Empig,': [645], 'Welte,': [648], 'Speck,': [651], 'Schmaljohn,': [653], 'Kreisberg,': [656], 'Goldsmith,': [659], '106,': [662], '117.': [663], '13J.': [664], 'W.': [665], 'Trauger,': [666], 'Baird,': [669], 'B.': [671, 1328], 'Dervan,': [672], 'Nature': [673], '1996,': [674, 1313], '382,': [675], '559.': [676], '14See': [677], 'Information.': [679], '15G.': [680], 'Bellomo,': [681], 'Vairetti,': [683], 'Stivala,': [685], 'Mirabelli,': [687], 'Richelmi,': [689], 'Orrenius,': [691], 'Proc.': [692, 1333, 1349], 'Natl.': [693, 1334, 1350], 'Acad.': [694, 1335, 1351], 'Sci.': [695, 1137, 1336, 1352], 'USA': [696, 1337, 1353], '1992,': [697], '89,': [698], '4412.': [699], '16Electron': [700], 'microscopy': [701], 'Philips': [706], 'EM410': [707], 'apparatus': [708, 1060], 'operated': [709], 'at': [710, 905, 1007, 1071, 1084, 1208], '80': [711], 'kV.': [712], 'Samples': [713], 'transferred': [715], 'onto': [716], 'ultrathin-carbon-film': [717], 'grids': [718], '(Ted': [719], 'Pella,': [720], 'CA,': [721], 'USA,': [722, 1172], '1822-F,': [723], 'formvar': [724], 'removed)': [725], 'placing': [727], 'grid': [729, 748, 757, 776, 802], 'top': [731], '10': [734], 'μL': [735], 'drop': [736, 764, 785], 'sample': [739], 'min.': [742], 'From': [743], 'one': [744], 'side': [745], 'excess': [750, 770, 794], 'fluid': [751, 771, 795], 'wicked': [753, 798], 'away,': [754, 799], 'then': [755, 986, 1025], 'placed': [759, 778], '100-μL': [762], 'water': [763], '30': [766], 's': [767], 'wash,': [769], 'again': [773, 797], 'removed,': [774], 'min': [781], '60-μL': [784], 'freshly': [787], 'filtered': [788], 'uranyl': [789], 'acetate': [790], '(1.33': [791], '%),': [792, 892], 'dried.': [805], '17The': [806], 'volume': [807, 857, 994], '5500-bp': [810], '11': [813], '800': [814], 'nm3': [815, 861], 'if': [816], 'hexagonal': [817], 'packing': [818], 'assumed,': [820, 852], 'analogy': [822], 'with:': [823], 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[910, 1012], '%-humidified': [911, 1013], 'CO2': [912], 'atmosphere.': [913, 1014], 'Overexpression': [914], 'receptor': [919], 'induced': [921], 'growing': [923], 'three': [927], 'passages': [928], 'depleted': [932], 'DMEM': [933, 1000, 1015], '(FDMEM).': [934], 'For': [935], 'flow': [936], 'cytometry,': [937], 'labeled': [940, 989], 'YOYO-1': [942], '(Molecular': [943], 'Probes,': [944], 'OR,': [945], 'USA;': [946], 'YOYO/150': [948], 'bp)': [949], 'mixing': [951], 'plasmid': [953], 'YOYO': [955], 'before': [956], 'addition': [957], 'detergent.': [960], 'Cells': [961, 1160], 'harvested': [963], 'trypsination,': [965], 'washed': [966], 'FDMEM,': [968], 'collected': [969, 1036, 1180], 'centrifugation,': [971], 'suspended': [973, 1040], 'serum-free': [975, 999], 'FDMEM.': [976], '(final': [979], 'concentration': [980], '105': [982], 'mixed': [987], 'mL': [997], 'incubated': [1003, 1146], '3': [1005, 1148], 'h': [1006, 1149], '(3': [1016], 'mL)': [1017], 'calf-thymus': [1019], '(200': [1021], 'account': [1028], 'nonspecific': [1030], 'binding': [1031], 'centrifugation': [1038], 'phosphate-buffered': [1042], 'saline': [1043], '(PBS)': [1044], 'bovine': [1046], 'albumin': [1048], '(BSA;': [1049], '%).': [1051], 'Flow': [1052], 'cytometric': [1053, 1158], 'analysis': [1054], 'FAC-StarPlus': [1059], '(Becton': [1061], 'Dickinson,': [1062], 'San': [1063], 'Jose,': [1064], 'USA)': [1065], 'an': [1067, 1182, 1190, 1231], 'argon': [1068], 'laser': [1069, 1185], '(excitation': [1070], '488': [1072], 'nm).': [1073, 1112], 'Granulation,': [1074], 'size,': [1075], 'fluorescence': [1077, 1100], 'intensity': [1078, 1101], '(emission': [1079], '515': [1080], 'nm)': [1081], 'recorded': [1083], 'rate': [1086], '300': [1088], 's−1.': [1090], 'Data': [1091], 'analyzed': [1093], 'using': [1094, 1105, 1196, 1256], 'cellQuest': [1095], 'software.': [1096, 1259], 'Calibration': [1097], '6-μm': [1106], 'calibrated': [1107], 'beads': [1108, 1122], '(λex=488': [1109], 'nm,': [1110], 'λem=515': [1111], 'Quantum': [1113], 'yields': [1114], 'FITC-labeled': [1116], 'BSA,': [1117], 'DNA-bound': [1118], 'YOYO,': [1119], 'calibration': [1121], 'measured': [1124], 'spectrofluorophotometer': [1127], '(Shimadzu': [1128], 'RF-5301PC);': [1129], 'FITC=fluorescein': [1130], 'isothiocyanate.': [1131], '19A.': [1132], 'Verkman,': [1134], 'Trends': [1135], 'Biochem.': [1136], '27,': [1139], '27.': [1140], '20KB': [1141], 'folate': [1142], 'ρ(+)': [1143], 'fluorescent': [1151], 'complexes': [1152], 'recovered': [1154], 'as': [1155], 'described[18]': [1156], 'analysis.': [1159], 'resuspended': [1161], 'FDMEM': [1163], 'deposited': [1165], 'chambered': [1167], 'cover': [1168], 'slides': [1169], '(Nalge': [1170], 'Nunc,': [1171], 'Lab-Tek': [1173], '155383).': [1174], 'Images': [1175], 'living': [1177], 'AMRC-1024ES': [1183], 'confocal': [1184], 'scanning': [1186], 'system': [1187], 'equipped': [1188], 'Eclipse': [1191], 'TE300': [1192], 'microscope': [1193], '(Nikon)': [1194], '60×': [1198], 'water-immersion': [1199], 'objective.': [1200], 'Series': [1201], '512×512': [1203], 'pixel': [1204], 'images': [1205], 'acquired': [1207], 'different': [1209], 'focal': [1210], 'planes': [1211], '(z': [1212], 'series)': [1213], 'displacement': [1216], 'mm': [1219], 'between': [1220], 'images.': [1221], 'Green': [1222], 'fluorescence,': [1223], 'obtained': [1224], '488-nm': [1227], 'excitation': [1228], 'line': [1229], 'argon/krypton': [1232], 'laser,': [1233], 'phase-contrast': [1235], 'micrographs': [1236], 'loaded': [1238], 'green': [1241], 'blue': [1243], 'channels': [1244], 'digital': [1247], 'color': [1248], 'image,': [1249], 'respectively.': [1250], 'Image': [1251], 'contrast': [1252], 'corrected': [1254], 'Adobe': [1257], 'Photoshop': [1258], '21J.': [1260], 'Zabner,': [1261], 'Fasbender,': [1264], 'Moninger,': [1266], 'Poellinger,': [1269], 'Welsh,': [1272], '1995,': [1276, 1338], '270,': [1277], '18': [1278], '997.': [1279], '22S.': [1280], 'Wang,': [1281], 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