Title: A rapid sensitive colorimetric method for the determination of ascorbic acid
Abstract: Abstract A new colorimetric procedure based on the interaction of ascorbic acid with 4,4′‐dimethoxydiquinone (I) in an aqueous dioxane solution was developed. In this procedure the diquinone reagent (I) is reduced to a blue‐violet product (II) 1 which absorbs maximally at 490–500 nm. The colour development takes place within 6 min at 25°C and absorbance vs concentration was linear up to a sample size of 600 μg. Reducing substances likely to be present in biological fluids or in natural extracts of the vitamin do not interfere with colour development and average recoveries of 99.8 ± 0.4% were obtained for solutions containing these substances.
Publication Year: 1976
Publication Date: 1976-01-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 1
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