Title: Expression and role of ionotropic glutamate receptors in pancreatic islet cells
Abstract: The FASEB JournalVolume 9, Issue 8 p. 686-691 Research Communication Expression and role of ionotropic glutamate receptors in pancreatic islet cells Nobuya Inagaki, Nobuya Inagaki Division of Molecular Medicine, Center for Biomedical Science, Chiba University School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba, 260 JapanSearch for more papers by this authorHiroshi Kuromi, Hiroshi Kuromi Division of Molecular Medicine, Center for Biomedical Science, Chiba University School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba, 260 JapanSearch for more papers by this authorTohru Gonoi, Tohru Gonoi Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260 JapanSearch for more papers by this authorYoshimasa Okamoto, Yoshimasa Okamoto Department of Metabolism and Clinical Nutrition, Kyoto University Faculty of Medicine, Yoshidakonoe-cho, Sakyo-ku, Kyoto, 606 JapanSearch for more papers by this authorHitoshi Ishida, Hitoshi Ishida Department of Metabolism and Clinical Nutrition, Kyoto University Faculty of Medicine, Yoshidakonoe-cho, Sakyo-ku, Kyoto, 606 JapanSearch for more papers by this authorYutaka Seino, Yutaka Seino Department of Metabolism and Clinical Nutrition, Kyoto University Faculty of Medicine, Yoshidakonoe-cho, Sakyo-ku, Kyoto, 606 JapanSearch for more papers by this authorTakeshi Kaneko, Takeshi Kaneko Department of Morphological Brain Science, Kyoto University Faculty of Medicine, Yoshidakonoe-cho, Sakyo-ku, Kyoto, 606 JapanSearch for more papers by this authorToshihiko Iwanaga, Toshihiko Iwanaga Department of Anatomy, Niigata University School of Medicine, 1-757, Asahimachi, Niigata, 951 JapanSearch for more papers by this authorSusumu Seino, Corresponding Author Susumu Seino n/[email protected] Division of Molecular Medicine, Center for Biomedical Science, Chiba University School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba, 260 JapanTo whom correspondence and reprint requests should be addressed, at: Division of Molecular Medicine, Center for Biomedical Science, Chiba University School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba 260, Japan.Search for more papers by this author Nobuya Inagaki, Nobuya Inagaki Division of Molecular Medicine, Center for Biomedical Science, Chiba University School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba, 260 JapanSearch for more papers by this authorHiroshi Kuromi, Hiroshi Kuromi Division of Molecular Medicine, Center for Biomedical Science, Chiba University School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba, 260 JapanSearch for more papers by this authorTohru Gonoi, Tohru Gonoi Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260 JapanSearch for more papers by this authorYoshimasa Okamoto, Yoshimasa Okamoto Department of Metabolism and Clinical Nutrition, Kyoto University Faculty of Medicine, Yoshidakonoe-cho, Sakyo-ku, Kyoto, 606 JapanSearch for more papers by this authorHitoshi Ishida, Hitoshi Ishida Department of Metabolism and Clinical Nutrition, Kyoto University Faculty of Medicine, Yoshidakonoe-cho, Sakyo-ku, Kyoto, 606 JapanSearch for more papers by this authorYutaka Seino, Yutaka Seino Department of Metabolism and Clinical Nutrition, Kyoto University Faculty of Medicine, Yoshidakonoe-cho, Sakyo-ku, Kyoto, 606 JapanSearch for more papers by this authorTakeshi Kaneko, Takeshi Kaneko Department of Morphological Brain Science, Kyoto University Faculty of Medicine, Yoshidakonoe-cho, Sakyo-ku, Kyoto, 606 JapanSearch for more papers by this authorToshihiko Iwanaga, Toshihiko Iwanaga Department of Anatomy, Niigata University School of Medicine, 1-757, Asahimachi, Niigata, 951 JapanSearch for more papers by this authorSusumu Seino, Corresponding Author Susumu Seino n/[email protected] Division of Molecular Medicine, Center for Biomedical Science, Chiba University School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba, 260 JapanTo whom correspondence and reprint requests should be addressed, at: Division of Molecular Medicine, Center for Biomedical Science, Chiba University School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba 260, Japan.Search for more papers by this author First published: 01 May 1995 https://doi.org/10.1096/fasebj.9.8.7768362Citations: 151AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Although the excitatory amino acid glutamate and its receptors play crucial roles in many functions of the central nervous system (CNS), their presence in the peripheral tissues has remained unclear. In the present study, we have identified kainate, α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and N-methyl-D-aspartate (NMDA) receptor subtype mRNAs in pancreatic islets, using reverse transcriptase polymerase chain reaction (RT-PCR). Intracellular calcium ([Ca2+]i) measurements and electrophysiological recordings indicate that kainate, AMPA, and NMDA all elicit increases of [Ca2+]i in single pancreatic β-cells and depolarize them. In addition, kainate and AMPA stimulate insulin secretion from isolated pancreatic islets, whereas NMDA does not. Also, immunocytochemical study shows the presence of intense glutaminase immunoreactivity in pancreatic α-cells and intrapancreatic ganglia, a finding compatible with the possibility that glutamate is released from α-cells as well as from neurons. Because the inhibitory amino acid γ-amino butyric acid (GABA) is present in β-cells as well as in neurons and inhibits glucagon secretion from α-cells, the present study suggests that glutamate and GABA are coordinated in the regulation of hormone secretion in pancreatic islets.—Inagaki, N., Kuromi, H., Gonoi, T., Okamoto, Y., Ishida, H., Seino, Y., Kaneko, T., Iwanaga, T., Seino, S. Expression and role of ionotropic glutamate receptors in pancreatic islet cells. FASEB J. 9, 686-691 (1995) Citing Literature Volume9, Issue8May 1995Pages 686-691 RelatedInformation
Publication Year: 1995
Publication Date: 1995-05-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 199
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