Publication Information

{'id': 'https://openalex.org/W2115782119', 'doi': 'https://doi.org/10.3791/2710', 'title': 'Seven Steps to Stellate Cells', 'display_name': 'Seven Steps to Stellate Cells', 'publication_year': 2011, 'publication_date': '2011-05-10', 'ids': {'openalex': 'https://openalex.org/W2115782119', 'doi': 'https://doi.org/10.3791/2710', 'mag': '2115782119', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/21610670', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/3197116'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.3791/2710', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S199738284', 'display_name': 'Journal of Visualized Experiments', 'issn_l': '1940-087X', 'issn': ['1940-087X'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320416', 'host_organization_name': 'MyJOVE', 'host_organization_lineage': ['https://openalex.org/P4310320416'], 'host_organization_lineage_names': ['MyJOVE'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'green', 'oa_url': 'https://europepmc.org/articles/pmc3197116?pdf=render', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5052284955', 'display_name': 'Patrick Maschmeyer', 'orcid': 'https://orcid.org/0000-0002-1705-5302'}, 'institutions': [{'id': 'https://openalex.org/I136199984', 'display_name': 'Harvard University', 'ror': 'https://ror.org/03vek6s52', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I136199984']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Patrick Maschmeyer', 'raw_affiliation_strings': ["Immune Disease Institute, Program in Cellular and Molecular Medicine at Children's Hospital, Department of Pathology, Harvard Medical School, Boston, MA, USA."], 'affiliations': [{'raw_affiliation_string': "Immune Disease Institute, Program in Cellular and Molecular Medicine at Children's Hospital, Department of Pathology, Harvard Medical School, Boston, MA, USA.", 'institution_ids': ['https://openalex.org/I136199984']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5041633349', 'display_name': 'Melanie Flach', 'orcid': 'https://orcid.org/0000-0002-0590-8320'}, 'institutions': [{'id': 'https://openalex.org/I136199984', 'display_name': 'Harvard University', 'ror': 'https://ror.org/03vek6s52', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I136199984']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Melanie Flach', 'raw_affiliation_strings': ["Immune Disease Institute, Program in Cellular and Molecular Medicine at Children's Hospital, Department of Pathology, Harvard Medical School"], 'affiliations': [{'raw_affiliation_string': "Immune Disease Institute, Program in Cellular and Molecular Medicine at Children's Hospital, Department of Pathology, Harvard Medical School", 'institution_ids': ['https://openalex.org/I136199984']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5089698093', 'display_name': 'Florian Winau', 'orcid': 'https://orcid.org/0000-0002-6825-1442'}, 'institutions': [{'id': 'https://openalex.org/I136199984', 'display_name': 'Harvard University', 'ror': 'https://ror.org/03vek6s52', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I136199984']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Florian Winau', 'raw_affiliation_strings': ["Immune Disease Institute, Program in Cellular and Molecular Medicine at Children's Hospital, Department of Pathology, Harvard Medical School"], 'affiliations': [{'raw_affiliation_string': "Immune Disease Institute, Program in Cellular and Molecular Medicine at Children's Hospital, Department of Pathology, Harvard Medical School", 'institution_ids': ['https://openalex.org/I136199984']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 1.434, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 68, 'citation_normalized_percentile': {'value': 0.896067, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 96, 'max': 97}, 'biblio': {'volume': None, 'issue': '51', 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10966', 'display_name': 'Liver physiology and pathology', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/2721', 'display_name': 'Hepatology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10966', 'display_name': 'Liver physiology and pathology', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/2721', 'display_name': 'Hepatology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T10351', 'display_name': 'Liver Disease Diagnosis and Treatment', 'score': 0.9956, 'subfield': {'id': 'https://openalex.org/subfields/2713', 'display_name': 'Epidemiology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T10337', 'display_name': 'Liver Disease and Transplantation', 'score': 0.9893, 'subfield': {'id': 'https://openalex.org/subfields/2721', 'display_name': 'Hepatology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/hepatic-stellate-cell', 'display_name': 'Hepatic stellate cell', 'score': 0.987111}, {'id': 'https://openalex.org/keywords/perisinusoidal-space', 'display_name': 'Perisinusoidal space', 'score': 0.8541051}, {'id': 'https://openalex.org/keywords/liver-cytology', 'display_name': 'Liver cytology', 'score': 0.6430833}, {'id': 'https://openalex.org/keywords/kupffer-cell', 'display_name': 'Kupffer cell', 'score': 0.41880926}], 'concepts': [{'id': 'https://openalex.org/C176891718', 'wikidata': 'https://www.wikidata.org/wiki/Q1997459', 'display_name': 'Hepatic stellate cell', 'level': 2, 'score': 0.987111}, {'id': 'https://openalex.org/C55450435', 'wikidata': 'https://www.wikidata.org/wiki/Q1229284', 'display_name': 'Perisinusoidal space', 'level': 4, 'score': 0.8541051}, {'id': 'https://openalex.org/C195521686', 'wikidata': 'https://www.wikidata.org/wiki/Q17152676', 'display_name': 'Liver cytology', 'level': 3, 'score': 0.6430833}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.54605365}, {'id': 'https://openalex.org/C2778394429', 'wikidata': 'https://www.wikidata.org/wiki/Q304956', 'display_name': 'Collagenase', 'level': 3, 'score': 0.45825377}, {'id': 'https://openalex.org/C142724271', 'wikidata': 'https://www.wikidata.org/wiki/Q7208', 'display_name': 'Pathology', 'level': 1, 'score': 0.4380268}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.4238099}, {'id': 'https://openalex.org/C2778904781', 'wikidata': 'https://www.wikidata.org/wiki/Q513486', 'display_name': 'Kupffer cell', 'level': 2, 'score': 0.41880926}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.32448035}, {'id': 'https://openalex.org/C2776200302', 'wikidata': 'https://www.wikidata.org/wiki/Q827450', 'display_name': 'Hepatocyte', 'level': 3, 'score': 0.22797197}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.19487679}, {'id': 'https://openalex.org/C71924100', 'wikidata': 'https://www.wikidata.org/wiki/Q11190', 'display_name': 'Medicine', 'level': 0, 'score': 0.1896396}, {'id': 'https://openalex.org/C203014093', 'wikidata': 'https://www.wikidata.org/wiki/Q101929', 'display_name': 'Immunology', 'level': 1, 'score': 0.1498549}, {'id': 'https://openalex.org/C134018914', 'wikidata': 'https://www.wikidata.org/wiki/Q162606', 'display_name': 'Endocrinology', 'level': 1, 'score': 0.1439211}, {'id': 'https://openalex.org/C202751555', 'wikidata': 'https://www.wikidata.org/wiki/Q221681', 'display_name': 'In vitro', 'level': 2, 'score': 0.12633678}, {'id': 'https://openalex.org/C2908801736', 'wikidata': 'https://www.wikidata.org/wiki/Q9368', 'display_name': 'Liver metabolism', 'level': 2, 'score': 0.0}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D055166', 'descriptor_name': 'Hepatic Stellate Cells', 'qualifier_ui': 'Q000166', 'qualifier_name': 'cytology', 'is_major_topic': True}, {'descriptor_ui': 'D008099', 'descriptor_name': 'Liver', 'qualifier_ui': 'Q000166', 'qualifier_name': 'cytology', 'is_major_topic': True}, {'descriptor_ui': 'D000818', 'descriptor_name': 'Animals', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017364', 'descriptor_name': 'Collagenases', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017364', 'descriptor_name': 'Collagenases', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D005904', 'descriptor_name': 'Glial Fibrillary Acidic Protein', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D055166', 'descriptor_name': 'Hepatic Stellate Cells', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D055166', 'descriptor_name': 'Hepatic Stellate Cells', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D008099', 'descriptor_name': 'Liver', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008297', 'descriptor_name': 'Male', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D051379', 'descriptor_name': 'Mice', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008810', 'descriptor_name': 'Mice, Inbred C57BL', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009419', 'descriptor_name': 'Nerve Tissue Proteins', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': False}, {'descriptor_ui': 'D009419', 'descriptor_name': 'Nerve Tissue Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011402', 'descriptor_name': 'Pronase', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D011402', 'descriptor_name': 'Pronase', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 4, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.3791/2710', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S199738284', 'display_name': 'Journal of Visualized Experiments', 'issn_l': '1940-087X', 'issn': ['1940-087X'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320416', 'host_organization_name': 'MyJOVE', 'host_organization_lineage': ['https://openalex.org/P4310320416'], 'host_organization_lineage_names': ['MyJOVE'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': True, 'landing_page_url': 'https://europepmc.org/articles/pmc3197116', 'pdf_url': 'https://europepmc.org/articles/pmc3197116?pdf=render', 'source': {'id': 'https://openalex.org/S4306400806', 'display_name': 'Europe PMC (PubMed Central)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1303153112', 'host_organization_name': 'European Bioinformatics Institute', 'host_organization_lineage': ['https://openalex.org/I1303153112'], 'host_organization_lineage_names': ['European Bioinformatics Institute'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3197116', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/21610670', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://europepmc.org/articles/pmc3197116', 'pdf_url': 'https://europepmc.org/articles/pmc3197116?pdf=render', 'source': {'id': 'https://openalex.org/S4306400806', 'display_name': 'Europe PMC (PubMed Central)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1303153112', 'host_organization_name': 'European Bioinformatics Institute', 'host_organization_lineage': ['https://openalex.org/I1303153112'], 'host_organization_lineage_names': ['European Bioinformatics Institute'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 7, 'referenced_works': ['https://openalex.org/W1963780536', 'https://openalex.org/W2008596165', 'https://openalex.org/W2016498524', 'https://openalex.org/W2039226303', 'https://openalex.org/W2039876496', 'https://openalex.org/W2111288662', 'https://openalex.org/W2164864100'], 'related_works': ['https://openalex.org/W4200078160', 'https://openalex.org/W3032766965', 'https://openalex.org/W2892107507', 'https://openalex.org/W2412070796', 'https://openalex.org/W2115782119', 'https://openalex.org/W2020223171', 'https://openalex.org/W2016498524', 'https://openalex.org/W1944631790', 'https://openalex.org/W1864790873', 'https://openalex.org/W1553693482'], 'abstract_inverted_index': {'Hepatic': [0], 'stellate': [1, 46, 67, 83, 112, 126, 136, 196, 204, 230, 268, 290, 307], 'cells': [2, 5, 21, 25, 47, 68, 84, 89, 94, 113, 127, 137, 205, 269], 'are': [3, 10, 26, 85, 148], 'liver-resident': [4], 'of': [6, 15, 37, 110, 124, 144, 185, 189, 203, 229, 233, 265, 274, 288, 295], 'star-like': [7], 'morphology': [8], 'and': [9, 22, 32, 90, 158, 175, 226, 299], 'located': [11], 'in': [12, 150, 178, 194], 'the': [13, 38, 71, 107, 122, 142, 153, 163, 181, 186, 201, 241, 262, 285, 306], 'space': [14, 143], 'Disse': [16], 'between': [17], 'liver': [18, 58, 164], 'sinusoidal': [19], 'endothelial': [20], 'hepatocytes1,2.': [23], 'Stellate': [24], 'derived': [27], 'from': [28, 114, 141, 206], 'bone': [29], 'marrow': [30], 'precursors': [31], 'store': [33], 'up': [34], 'to': [35, 51, 57, 64, 118, 134, 139, 168, 250, 304], '80%': [36], 'total': [39], 'body': [40], 'vitamin': [41, 190], 'A1,': [42], '2.': [43], 'Upon': [44], 'activation,': [45], 'differentiate': [48], 'into': [49], 'myofibroblasts': [50], 'produce': [52], 'extracellular': [53], 'matrix,': [54], 'thus': [55], 'contributing': [56], 'fibrosis3.': [59], 'Based': [60], 'on': [61, 213], 'their': [62, 119, 296], 'ability': [63], 'contract,': [65], 'myofibroblastic': [66], 'can': [69, 91, 235], 'regulate': [70], 'vascular': [72], 'tone': [73], 'associated': [74], 'with': [75, 152, 172], 'portal': [76], 'hypertension4.': [77], 'Recently,': [78], 'we': [79, 102, 279], 'demonstrated': [80], 'that': [81, 270, 302], 'hepatic': [82, 111, 125, 195, 208, 267, 289], 'potent': [86], 'antigen': [87], 'presenting': [88], 'activate': [92], 'NKT': [93], 'as': [95, 97], 'well': [96], 'conventional': [98], 'T': [99], 'lymphocytes5.': [100], 'Here': [101], 'present': [103, 280], 'a': [104, 129, 223, 281], 'method': [105, 182], 'for': [106, 240, 284], 'efficient': [108, 286], 'preparation': [109], 'mouse': [115, 146], 'liver.': [116], 'Due': [117], 'perisinusoidal': [120], 'localization,': [121], 'isolation': [123, 140, 287], 'is': [128, 166], 'multi-step': [130], 'process.': [131], 'In': [132], 'order': [133], 'render': [135], 'accessible': [138], 'Disse,': [145], 'livers': [147], 'perfused': [149], 'situ': [151], 'digestive': [154], 'enzymes': [155], 'Pronase': [156, 173], 'E': [157, 174], 'Collagenase': [159, 176], 'P.': [160], 'Following': [161], 'perfusion,': [162], 'tissue': [165], 'subjected': [167], 'additional': [169], 'enzymatic': [170], 'treatment': [171], 'P': [177], 'vitro.': [179], 'Subsequently,': [180], 'takes': [183], 'advantage': [184], 'massive': [187], 'amount': [188], 'A-storing': [191], 'lipid': [192, 275], 'droplets': [193], 'cells.': [197, 231], 'This': [198], 'feature': [199], 'allows': [200], 'separation': [202], 'other': [207], 'cell': [209, 308], 'types': [210], 'by': [211, 238, 252], 'centrifugation': [212], 'an': [214], '8%': [215], 'Nycodenz': [216], 'gradient.': [217], 'The': [218], 'protocol': [219, 283], 'described': [220], 'here': [221], 'yields': [222], 'highly': [224], 'pure': [225], 'homogenous': [227], 'population': [228], 'Purity': [232], 'preparations': [234], 'be': [236], 'assessed': [237], 'staining': [239], 'marker': [242], 'molecule': [243], 'glial': [244], 'fibrillary': [245], 'acidic': [246], 'protein': [247], '(GFAP),': [248], 'prior': [249], 'analysis': [251], 'fluorescence': [253], 'microscopy': [254, 260], 'or': [255], 'flow': [256], 'cytometry.': [257], 'Further,': [258], 'light': [259], 'reveals': [261], 'unique': [263], 'appearance': [264, 298], 'star-shaped': [266], 'harbor': [271], 'high': [272], 'amounts': [273], 'droplets.': [276], 'Taken': [277], 'together,': [278], 'detailed': [282], 'cells,': [291], 'including': [292], 'representative': [293], 'images': [294], 'morphological': [297], 'GFAP': [300], 'expression': [301], 'help': [303], 'define': [305], 'entity.': [309]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2115782119', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}, {'year': 2023, 'cited_by_count': 5}, {'year': 2022, 'cited_by_count': 3}, {'year': 2021, 'cited_by_count': 6}, {'year': 2020, 'cited_by_count': 4}, {'year': 2019, 'cited_by_count': 7}, {'year': 2018, 'cited_by_count': 7}, {'year': 2017, 'cited_by_count': 7}, {'year': 2016, 'cited_by_count': 8}, {'year': 2015, 'cited_by_count': 8}, {'year': 2014, 'cited_by_count': 7}], 'updated_date': '2025-01-02T14:16:16.365274', 'created_date': '2016-06-24'}