Title: Inactivation of the protease inhibitor phenylmethylsulfonyl fluoride in buffers
Abstract: Aqueous preparations of phenylmethylsulfonyl fluoride (PMSF) become inactive toward proteases unless promptly brought into contact with protease. Inactivation of PMSF increases with increased pH and temperature. Half-lives of the inhibitor at 25°C are approximately 110,55, and 35 min at pH 7.0, 7.5, and 8.0, respectively. At pH 8, 100 μm PMSF is almost completely inactivated within 1 hr at 25°C or within 22 hr at 4°C. Stock solutions of PMSF in 100% isopropanol are stable at 25°C for months if not longer. Reactivation of PMSF-inhibited chymotrypsin did not occur within 1 week at 25°C at pH 7.0.
Publication Year: 1978
Publication Date: 1978-06-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 320
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