Title: Proteomic analysis of tobacco mosaic virus-infected tomato (Lycopersicon esculentum M.) fruits and detection of viral coat protein
Abstract: PROTEOMICSVolume 6, Issue S1 p. S196-S206 Research Article Proteomic analysis of tobacco mosaic virus-infected tomato (Lycopersicon esculentum M.) fruits and detection of viral coat protein Juan Casado-Vela, Juan Casado-Vela Grupo de Proteómica y Genómica Funcional de Plantas, Departamento de Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Alicante, SpainSearch for more papers by this authorSusana Sellés, Susana Sellés Grupo de Proteómica y Genómica Funcional de Plantas, Departamento de Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Alicante, SpainSearch for more papers by this authorRoque Bru Martínez Professor, Corresponding Author Roque Bru Martínez Professor [email protected] Grupo de Proteómica y Genómica Funcional de Plantas, Departamento de Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Alicante, SpainGrupo de Proteómica y Genómica Funcional de Plantas, Departamento de Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Campus de San Vicente del Raspeig, Apdo. 99, 03080 Alicante, Spain Fax: +34-965903880===Search for more papers by this author Juan Casado-Vela, Juan Casado-Vela Grupo de Proteómica y Genómica Funcional de Plantas, Departamento de Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Alicante, SpainSearch for more papers by this authorSusana Sellés, Susana Sellés Grupo de Proteómica y Genómica Funcional de Plantas, Departamento de Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Alicante, SpainSearch for more papers by this authorRoque Bru Martínez Professor, Corresponding Author Roque Bru Martínez Professor [email protected] Grupo de Proteómica y Genómica Funcional de Plantas, Departamento de Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Alicante, SpainGrupo de Proteómica y Genómica Funcional de Plantas, Departamento de Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Campus de San Vicente del Raspeig, Apdo. 99, 03080 Alicante, Spain Fax: +34-965903880===Search for more papers by this author First published: 18 April 2006 https://doi.org/10.1002/pmic.200500317Citations: 51AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Tobacco mosaic virus (TMV) is a widespread plant virus from the genus Tobamovirus that affects tobacco and tomato plants causing a pathology characterised by cell breakage and disorganisation in plant leaves and fruits. In this study we undertook a proteomic approach to investigate the molecular and biochemical mechanisms potentially involved in tomato fruit defence against the viral infection. The comparison of 2-D gels from control and TMV-infected but asymptomatic tomato fruits revealed changes in several proteins. The differential expression of peptidases, endoglucanase, chitinase and proteins participating in the ascorbate-glutathione cycle in infected fruits suggests that pathogenesis-related proteins and antioxidant enzymes may play a role in the protection against TMV infection. TMV coat protein appeared as a prominent spot in 2-D gels from TMV-infected asymptomatic fruits. A Triton X-114 phase-partitioning step of tomato protein extracts favoured the solubilisation of TMV coat protein and the enrichment of two aminopeptidases not present in control fruits. PMF and MS/MS data of the 2-D gel-isolated TMV coat protein is proposed as a powerful analysis method for the simultaneous tobamovirus detection, species determination and strain differentiation in virus-infected fruit commodities. References 1 M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop (Eds.), Virus Taxonomy. Classification and Nomenclature of Viruses: Seventh Report of the International Committee on Taxonomy of Viruses, Academic Press, San Diego 2000. 2 Alonso, E., Luque, I. G., Avilarincon, M. J., Wicke, B. et. al., J. Phytopathol. 1989, 125, 67–76. 3 Letscher, B., Adam, G., Lesemann, D. E., Willingham, P. et al., J. Virol. Methods 2002, 106, 1–10. 4 Creager, A. N., Scholthof, K. B., Citovsky, V., Scholthof, H. 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Citing Literature Volume6, IssueS1Supplement: PROTEOMICS IN SPAIN Proceedings of the 1st Congress of the Spanish Proteomics Society Cordoba, Spain, 14–17 February 2005No. S 1 April 2006Pages S196-S206 ReferencesRelatedInformation
Publication Year: 2006
Publication Date: 2006-03-13
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 62
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