Publication Information

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'arthritis.': [35, 265, 1074], 'To': [36, 266, 2694], 'clone': [37, 267, 3523], 'molecules': [38, 268, 478, 1382], 'that': [39, 96, 193, 211, 269, 326, 423, 441, 878, 960, 1377, 1413, 1463, 3450, 3842, 3890, 4038, 4117, 4137, 4174], 'bind': [40, 270, 3866], 'ADAMTS4,': [42, 133, 272, 363, 2011, 3453, 3747, 3845, 3966, 4094, 4216], 'we': [43, 273, 1409, 1433], 'screened': [44, 274, 3465], 'human': [46, 276, 1405, 1491, 1601, 1898, 3460, 3507], 'chondrocyte': [47, 277, 1406, 1492, 1602, 1705, 3461], 'cDNA': [48, 64, 278, 294, 1407, 1493, 1502, 1603, 1670, 1674, 1706, 2021, 2149, 2185, 3462, 3473, 3571], 'library': [49, 279, 1494, 1604, 1707, 3463], 'by': [50, 76, 121, 157, 201, 280, 306, 351, 387, 431, 906, 964, 1024, 1082, 1383, 1402, 1513, 1701, 1871, 1879, 1938, 1957, 1990, 2075, 2226, 2316, 2466, 2524, 2559, 2562, 2617, 2740, 2777, 2814, 3030, 3140, 3148, 3161, 3271, 3299, 3372, 3422, 3466, 3555, 3688, 3731, 3819, 3992, 4010, 4091, 4161, 4208, 4273, 4320], 'yeast': [52, 80, 282, 310, 1441, 3468, 3556], 'two-hybrid': [53, 81, 283, 311, 1442, 1485, 3469, 3557], 'system': [54, 284, 1443, 1486, 3212, 3470], 'using': [55, 87, 285, 317, 1515, 1678, 1703, 1852, 2077, 2191, 2262, 2545, 2722, 2755, 3150, 3206, 3318, 3375, 3429, 3471, 3852, 4016, 4322], 'spacer': [58, 109, 143, 288, 339, 373, 1224, 1285, 1352, 1423, 1507, 2044, 2056, 2066, 3478, 3923, 3931, 3938, 4177], 'domain': [59, 99, 110, 289, 329, 340, 1353, 1424, 1436, 1508, 1921, 2045, 2053, 2057, 3479, 3533, 4076, 4178], 'as': [60, 119, 290, 349, 504, 744, 1131, 1437, 1533, 1708, 2079, 2105, 2474, 2806, 2984, 3092, 3287, 3406, 3486, 3506, 3598, 3600, 3602, 3611], 'bait': [61, 291, 1438], 'obtained': [63, 293], 'clones': [65, 295, 3457, 3491, 3502, 3511, 3590, 3604, 3634], 'derived': [66, 296, 1503], 'from': [67, 297, 1497, 1504, 1527, 2228, 2237, 2811, 3036, 3458, 3793, 3955], 'fibronectin.': [68, 298, 2728, 3508, 3740, 3827, 4188], 'Interaction': [69, 299, 3538], 'between': [70, 300, 1474, 2767, 4148], 'fibronectin': [73, 89, 101, 118, 158, 173, 202, 303, 319, 331, 348, 388, 403, 432, 1446, 1464, 1695, 1798, 1900, 1915, 2628, 2768, 2780, 2852, 3224, 3244, 3307, 3363, 3535, 3552, 3582, 3638, 3764, 3841, 4152, 4205, 4228, 4246, 4282, 4302, 4331], 'was': [74, 115, 154, 166, 304, 345, 384, 396, 1511, 1568, 1647, 1850, 1877, 1895, 1955, 1974, 1986, 2013, 2027, 2072, 2154, 2557, 2701, 2753, 2771, 3056, 3138, 3158, 3268, 3285, 3297, 3348, 3420, 3436, 3553, 3562, 3666, 3686, 3728, 3807, 3814, 3850, 3886, 4008, 4089, 4114, 4132, 4153, 4158, 4206, 4229, 4242, 4255, 4318], 'demonstrated': [75, 95, 305, 325, 959], 'chemical': [77, 307], 'cross-linking.': [78, 308], 'A': [79, 309, 3859], 'assay': [82, 86, 312, 316, 2549, 2721, 2776, 3849, 4088], 'solid-phase': [84, 314, 3413, 3847, 4086], 'binding': [85, 105, 315, 335, 1279, 1381, 1411, 1886, 2720, 2775, 2910, 3414, 3548, 3848, 4082, 4087, 4108, 4127, 4147, 4157, 4185], 'wild-type': [88, 152, 180, 318, 382, 410, 2010, 3226, 3295, 4031, 4130, 4215, 4240, 4284], 'their': [93, 206, 323, 436], 'mutants': [94, 324, 2190, 2223, 2463, 2681, 3914, 4142], 'C-terminal': [98, 108, 171, 207, 328, 338, 401, 437, 1172, 1223, 1282, 1351, 1476, 1506, 2626, 3477, 3530, 3833, 3922, 4067, 4176], 'capable': [103, 333], 'ADAMTS4.': [112, 342, 1398], 'Wild-type': [113, 343], 'co-localized': [116, 346], 'determined': [120, 350, 1677, 2544, 4319], 'confocal': [122, 352, 2997], 'microscopy': [123, 353], 'on': [124, 354, 1616, 1810, 1951, 2883, 3201, 3312, 3493, 3619, 3983, 4192, 4231, 4247, 4283], 'cell': [126, 356, 1880, 2840, 4233, 4249], 'surface': [127, 357, 4234], 'stable': [129, 359, 4212], '293T': [130, 360, 2976, 3948], 'transfectants': [131, 361, 2216, 2239, 2797, 4213, 4263], 'expressing': [132, 362, 2217, 2798, 2968, 3605, 3635, 4214], 'although': [134, 364, 980], 'deletion': [136, 366, 2680], 'mutants,': [137, 367, 2039], 'including': [138, 368], 'ΔSp': [139, 369, 2041, 2114, 2408, 2519, 3229, 4034], '(ΔArg693–Lys837,': [140, 370], 'lacking': [141, 371, 1618, 1812, 1823, 2042, 2048, 2061, 3495, 3621], 'domain),': [144, 374], 'showed': [145, 375, 3234, 3641, 3697, 3782, 3874, 3971, 4053], 'negligible': [146, 376, 3643, 3783, 4251], 'localization.': [147, 377], 'The': [148, 170, 378, 400, 1075, 1457, 1561, 1590, 1765, 1793, 1844, 1868, 1953, 1984, 2098, 2311, 2462, 2537, 2552, 2644, 2861, 2896, 3111, 3365, 3878, 4145], 'aggrecanase': [149, 195, 379, 425, 1076, 1330, 1467, 3136, 3236, 3283, 3292, 4001, 4046, 4293, 4316], 'activity': [150, 178, 196, 218, 380, 408, 426, 448, 910, 1077, 1280, 1331, 1345, 1386, 1468, 1846, 1869, 2831, 3137, 3284, 3293, 3314, 3682, 3700, 4002, 4061, 4109, 4128, 4317], 'dose-dependently': [155, 385], 'inhibited': [156, 176, 200, 386, 406, 430, 1081, 3298, 4160], '(IC50': [159, 182, 389, 412], '=': [160, 183, 390, 413], '110': [161, 391], 'nm),': [162, 392], 'whereas': [163, 393, 3871], 'no': [164, 394, 1390, 4060, 4253], 'inhibition': [165, 395], 'observed': [167, 397, 4256], 'ΔSp.': [169, 399], '40-kDa': [172, 402, 1173, 2627], 'fragment': [174, 404, 1160, 1174, 1722, 1730, 1738, 1746, 1754, 1763, 1783, 1916, 2629, 3474, 3573, 4023], 'also': [175, 405, 2979, 3019, 3316], '170': [184, 414], 'nm).': [185, 415], 'These': [186, 416, 2137, 3509, 3950], 'data': [187, 417, 1375, 1458], 'demonstrate': [188, 418, 1462], 'for': [189, 222, 419, 452, 1245, 1289, 1394, 1451, 1641, 1721, 1729, 1737, 1745, 1753, 1762, 1774, 1782, 1841, 1930, 1948, 1965, 2102, 2113, 2123, 2134, 2202, 2212, 2418, 2638, 2664, 2688, 2822, 2855, 2893, 2937, 3005, 3011, 3021, 3075, 3257, 3276, 3520, 3663], 'first': [191, 421], 'time': [192, 422], 'through': [203, 433, 901, 1339, 1471], 'interaction': [204, 434, 1340, 1473, 2766, 3720, 4200], 'domains': [208, 438, 1286, 2067], 'suggest': [210, 440, 1376, 4173], 'this': [212, 442, 1189, 1400, 1460], 'extracellular': [213, 443, 1149, 1246], 'regulation': [214, 444], 'mechanism': [215, 445, 1194], 'may': [219, 449, 1379, 1414], 'be': [220, 450, 1415, 1448, 3817], 'important': [221, 451, 1061], 'aggrecan': [226, 456, 881, 1065, 1293, 2770, 3142, 4013, 4022], 'arthritic': [228, 458, 896], 'cartilage.': [229, 459], 'Members': [460], 'family': [471], 'are': [472, 495, 899, 3404], 'composed': [473], 'at': [475, 903, 1838, 1874, 1933, 1968, 2641, 2667, 2691, 2825, 2858, 2940, 2999, 3078, 3260, 3279, 3324, 3692, 3750], 'least': [476], '19': [477], '(1Llamazares': [479], 'M.': [480, 532, 677, 721, 859, 934, 1004, 1039, 1095, 1097, 3175], 'Cal': [481], 'S.': [482, 723, 861], 'Quesada': [483], 'V.': [484, 643], 'Lopez-Otin': [485], 'C.': [486, 703, 841, 990, 1302], 'J.': [487, 549, 693, 730, 868, 922, 937, 1013, 1048, 1102, 1234, 1260, 1269, 1321, 1357, 1366, 1554, 2512, 2590, 3182], 'Biol.': [488, 731, 869, 938, 1103, 1235, 1270, 1322, 1367, 1555, 2513, 2591], 'Chem.': [489, 732, 870, 939, 1104, 1236, 1271, 1323, 1368, 1556, 2514, 2592], '2003;': [490, 733, 871], '278:': [491, 734, 872], '13382-13389Google': [492], 'Scholar)': [493, 3055], 'involved': [496, 1416, 4080, 4183], 'various': [498], 'biological': [499], 'biochemical': [501], 'events': [502], 'such': [503], 'fertilization,': [505], 'degradation,': [507], 'processing': [508], 'fibrillar': [510], 'collagens,': [511], 'intravascular': [513], 'coagulation': [514], '(2Shindo': [515], 'T.': [516, 524, 528, 542, 625, 1112, 1308, 1312, 2585, 3043, 3098, 3388], 'Kurihara': [517, 525], 'H.': [518, 522, 534, 540, 585, 589, 595, 647, 803, 807, 813, 1099, 1114, 1537, 1547, 3045, 3100, 3390], 'Kuno': [519], 'K.': [520, 548, 583, 599, 668, 801, 817, 1101, 1116, 1231, 1233, 1543, 1545, 2499, 3047, 3102, 3173, 3179, 3181, 3392], 'Yokoyama': [521], 'Wada': [523], 'Y.': [526, 530, 538, 546, 591, 681, 809, 1000, 1035, 1118, 1539, 1551, 1553, 2501, 2507, 2511, 2583, 2589, 3049, 3104, 3177, 3394], 'Imai': [527], 'Wang': [529], 'Ogata': [531], 'Nishimatsu': [533], 'Moriyama': [535], 'N.': [536, 2509], 'Oh-hashi': [537], 'Morita': [539], 'Ishikawa': [541], 'Nagai': [543], 'R.': [544, 575, 587, 637, 639, 727, 793, 805, 865, 1549], 'Yazaki': [545], 'Matsushima': [547, 1232], 'Clin.': [550], 'Investig.': [551], '2000;': [552, 1557], '105:': [553], '1345-1352Google': [554], 'Scholar,': [555, 617, 673, 697, 711, 835, 849, 943, 998, 1108], '3Tortorella': [556], 'M.D.': [557, 699, 775, 837, 986, 1008, 1043], 'Burn': [558, 776], 'T.C.': [559, 777], 'Pratta': [560, 778], 'M.A.': [561, 779], 'Abbaszade': [562, 780], 'I.': [563, 781, 1541, 2503, 2581], 'Hollis': [564, 603, 782, 821], 'J.M.': [565, 610, 715, 719, 783, 828, 853, 857], 'Liu': [566, 784], 'R.-Q.': [567, 785], 'Rosenfeld': [568, 786], 'S.A.': [569, 787, 1310], 'Copeland': [570, 788], 'R.A.': [571, 789, 928, 1006, 1041], 'Decicco': [572, 790], 'C.P.': [573, 791], 'Wynn': [574, 792], 'Rockwell': [576, 794], 'A.': [577, 679, 795], 'Yang': [578, 630, 796], 'F.': [579, 797], 'Duke': [580, 798], 'J.L.': [581, 799], 'Solomon': [582, 800], 'George': [584, 802], 'Bruckner': [586, 804], 'Nagase': [588, 806, 1098], 'Itoh': [590, 808], 'Ellis': [592, 810], 'D.M.': [593, 811], 'Ross': [594, 720, 812, 858], 'Wiswall': [596, 814], 'B.H.': [597, 815], 'Murphy': [598, 816], 'Hillman': [600, 818], 'Jr.,': [601, 651, 819], 'M.C.': [602, 820], 'G.F.': [604, 822], 'Newton': [605, 823], 'R.C.': [606, 824], 'Magolda': [607, 825], 'R.L.': [608, 826], 'Trzaskos': [609, 827], 'Arner': [611, 704, 829, 842, 991, 1009, 1044], 'E.C.': [612, 623, 830, 1010, 1045], 'Science.': [613, 831], '1999;': [614, 832, 3185], '284:': [615, 833], '1664-1666Google': [616, 834], '4Levy': [618], 'G.G.': [619], 'Nichols': [620], 'W.C.': [621], 'Lian': [622], 'Foroud': [624], 'McClintick': [626], 'J.N.': [627], 'McGee': [628], 'B.M.': [629], 'A.Y.': [631], 'Siemieniak': [632], 'D.R.': [633], 'Stark': [634], 'K.R.': [635], 'Gruppo': [636], 'Sarode': [638], 'Shurin': [640], 'S.B.': [641], 'Chandrasekaran': [642], 'Stabler': [644], 'S.P.': [645], 'Sabio': [646], 'Bouhassira': [648], 'E.E.': [649], 'Upshaw': [650], 'J.D.': [652, 936, 949, 1268, 1365], 'Ginsburg': [653], 'D.': [654, 660], 'Tsai': [655], 'H.M.': [656], 'Zheng': [657], 'X.': [658], 'Chung': [659], 'Takayama': [661], 'T.K.': [662, 685, 1314], 'Majerus': [663], 'E.M.': [664], 'Sadler': [665], 'J.E.': [666], 'Fujikawa': [667], 'Nature.': [669], '2001;': [670, 694, 708, 846, 995, 1105, 1121, 3052, 3107, 3397], '413:': [671], '488-494Google': [672], '5Li': [674], 'S.W.': [675], 'Arita': [676], 'Fertala': [678], 'Bao': [680], 'Kopen': [682], 'G.C.': [683], 'Langsjo': [684], 'Hyttinen': [686], 'M.M.': [687], 'Helminen': [688], 'H.J.': [689], 'Prockop': [690], 'D.J.': [691], 'Biochem.': [692, 3183], '355:': [695], '271-278Google': [696], '6Tortorella': [698, 836], 'Malfait': [700, 838, 987], 'A.M.': [701, 839, 988], 'Deccico': [702, 840, 989], 'E.': [705, 843, 992, 2505, 2587], 'Osteoarthritis': [706, 844, 993], 'Cartilage.': [707, 845, 994], '9:': [709, 847, 996], '539-552Google': [710, 848, 997], '7Somerville': [712, 850], 'R.P.': [713, 851], 'Longpre': [714, 852], 'Jungers': [716, 854], 'K.A.': [717, 855], 'Engle': [718, 856], 'Evanko': [722, 860], 'Wight': [724, 862], 'T.N.': [725, 863], 'Leduc': [726, 864], 'Apte': [728, 866], 'S.S.': [729, 867, 932], '9503-9513Google': [735, 873], 'Scholar).': [736, 874, 955, 1018, 1053, 1275, 1327, 1372, 1560, 1867, 2518, 2596, 3110, 3188, 3400], 'Among': [737, 3488], 'them,': [738], 'ADAMTS1,': [739], '(also': [741], 'referred': [742], 'aggrecanase-1),': [745], 'ADAMTS5': [746, 981], '(aggrecanase-2),': [747], 'ADAMTS9': [749], 'cleave': [750], 'specific': [751, 2697, 4146], 'Glu–X': [752, 904], 'bonds': [753, 905], '(where': [754], 'X': [755], 'most': [757, 2049, 3925], 'often': [758], 'Ala': [759], 'or': [760, 976, 1903, 1911, 2188, 2220, 2330, 2634, 2678, 2706, 2713, 2800, 2929, 3064, 3070, 3579, 4097, 4220, 4252, 4261], 'Gly)': [761], 'core': [764], 'protein': [765, 2548, 3973], 'aggrecan,': [767], 'major': [769, 880, 3972], 'articular': [772, 1068], '(3Tortorella': [774], 'Previous': [875], 'studies': [876, 957], 'suggested': [877], 'fragments': [882, 1689, 1775, 3143, 3308, 3323, 3639], 'found': [883, 1445], 'vitro': [885], 'response': [887], 'cytokine-stimulated': [889], 'vivo': [894], 'joint': [897], 'fluids': [898], 'generated': [900], 'cleavage': [902, 1205], 'glutamyl': [908], 'endopeptidase': [909], 'these': [912, 1374, 2238, 2540, 3631, 4039, 4111, 4171, 4295], 'AD-AMTS': [913], 'members': [914], '(8Lark': [915], 'M.W.': [916], 'Gordy': [917], 'J.T.': [918], 'Weidner': [919], 'J.R.': [920], 'Ayala': [921], 'Kimura': [923], 'J.H.': [924], 'Williams': [925], 'H.R.': [926], 'Mumford': [927], 'Flannery': [929], 'C.R.': [930, 1298], 'Carlson': [931], 'Iwata': [933], 'Sandy': [935, 948, 1267, 1364], '1995;': [940, 2515], '270:': [941, 2516], '2550-2556Google': [942], '9Lohmander': [944], 'L.S.': [945], 'Neame': [946], 'P.J.': [947], 'Arthritis': [950], 'Rheum.': [951], '1993;': [952], '36:': [953], '1214-1222Google': [954], 'Recent': [956], 'further': [958, 3717], 'induced': [963], 'stimulation': [965], 'chondrocytes': [967, 1028], 'synovial': [969, 1025], 'cells': [970, 1026, 2163, 2199, 2862, 2879, 2897, 2967, 2977], 'interleukin-1,': [972], 'tumor': [973], 'necrosis': [974], 'factor-α,': [975], 'transforming': [977], 'growth': [978, 3645], 'factor-β,': [979], 'constitutively': [983], 'expressed': [984, 3405, 3944], '(6Tortorella': [985], '10Yamanishi': [999], 'Boyle': [1001, 1036], 'D.L.': [1002, 1037], 'Clark': [1003, 1038], 'Maki': [1005, 1040], 'Tortorella': [1007, 1042, 1096], 'Firestein': [1011, 1046], 'G.S.': [1012, 1047], 'Immunol.': [1014, 1049], '2002;': [1015, 1050, 1272, 1324, 1369, 2593], '168:': [1016, 1051], '1405-1412Google': [1017, 1052], 'In': [1019, 1276, 1399, 3411, 3628], 'addition,': [1020], 'overexpressed': [1023], 'arthritis': [1033], '(10Yamanishi': [1034], 'Thus,': [1054, 1373, 3810, 4170], 'an': [1060, 1243, 2263, 2296, 2457, 2993, 3775, 4020], 'TIMP-3': [1083], '(tissue': [1084], 'inhibitor': [1085], 'metalloproteinases-3)': [1087], 'among': [1088, 1430], 'four': [1090], 'TIMP': [1091], 'proteins': [1092, 1395, 1412, 2313, 2556, 2572, 2737, 3200, 3449], '(TIMP-1–4)': [1093], '(11Kashiwagi': [1094], 'Brew': [1100], '276:': [1106], '12501-12504Google': [1107], '12Hashimoto': [1109], 'G.': [1110, 1258, 1355, 2579, 3041, 3096, 3386], 'Aoki': [1111, 2584, 3042, 3097, 3387], 'Nakamura': [1113, 3044, 3099, 3389], 'Tanzawa': [1115, 1544, 3046, 3101, 3167, 3180, 3391], 'Okada': [1117, 1552, 2510, 2588, 3048, 3103, 3393], 'FEBS': [1119, 3050, 3105, 3395], 'Lett.': [1120, 3051, 3106, 3396], '494:': [1122, 3053, 3108, 3398], '192-195Google': [1123, 3054, 3109, 3399], 'Scholar),': [1124, 1240], 'all': [1125, 3516, 4236], 'which': [1127, 1384, 2411, 3157, 3233, 3347, 3748, 3919, 4075, 4120, 4290], 'were': [1128, 1495, 1592, 1612, 1630, 1653, 1671, 1676, 1699, 1768, 1801, 1807, 1819, 2084, 2104, 2139, 2164, 2200, 2224, 2240, 2260, 2314, 2332, 2372, 2426, 2454, 2464, 2522, 2543, 2615, 2646, 2671, 2738, 2804, 2842, 2863, 2880, 2898, 2944, 2978, 2990, 3018, 3114, 3129, 3204, 3238, 3315, 3370, 3427, 3464, 3504, 3943, 3953, 3990, 4298], 'originally': [1129], 'cloned': [1130, 1580, 1787, 2091], 'inhibitors': [1132], 'matrix': [1134, 1142, 1247], 'metalloproteinases': [1135], '(MMPs).': [1136], '1The': [1137], 'abbreviations': [1138], 'used': [1139, 1434, 2101], 'are:': [1140], 'MMPs,': [1141], 'metalloproteinases;': [1143], 'TSP,': [1144, 2063, 3935], 'thrombospondin-1;': [1145], 'CR,': [1146, 2064, 3936], 'cysteine-rich;': [1147], 'ECM,': [1148], 'matrix;': [1150], 'X-α-gal,': [1151], '5-bromo-4-chloro-3-indolyl-α-d-galactopyranoside;': [1152], 'PBS,': [1153], 'phosphate-buffered': [1154, 1927], 'saline;': [1155], 'Fn-f120,': [1156, 3856], 'central': [1157, 1919], 'cell-binding': [1158, 1920], '120-kDa': [1159, 1914], 'fibronectin;': [1162, 1176], 'DSS,': [1163, 3802], 'disuccinimidyl': [1164, 1945], 'suberate;': [1165], 'DMEM,': [1166], "Dulbecco's": [1167, 2167], 'modified': [1168, 2168], "Eagle's": [1169, 2169], 'medium;': [1170], 'Fn-f40,': [1171], 'BSA,': [1177, 4119], 'bovine': [1178, 2175, 2657, 2837, 3037], 'serum': [1179, 2176, 2658, 2904], 'albumin;': [1180], 'MT,': [1181], 'membrane-type.': [1182], 'However,': [1183, 1389, 4125], 'it': [1184], 'not': [1186, 1428, 2413, 3808, 3884], 'known': [1187], 'whether': [1188], 'only': [1192, 3642, 3875, 4054, 4122], 'regulatory': [1193], 'activity.': [1197, 1420, 1456, 4047], 'consists': [1199], 'prodomain,': [1202], 'furin': [1204], 'site,': [1206], 'catalytic': [1208], 'domain,': [1209, 1220], 'disintegrin-like': [1211], 'motif,': [1212, 1216], 'thrombospondin-1': [1214], '(TSP)': [1215], 'cysteine-rich': [1218], '(CR)': [1219], 'domain.': [1225], 'As': [1226, 3585, 3741, 3959, 4102, 4223, 4326], 'shown': [1227, 3586, 3742, 3960, 4103, 4224, 4327], 'ADAMTS1': [1229], '(13Kuno': [1230], '1998;': [1237], '273:': [1238], '13912-13917Google': [1239], 'has': [1242, 1294], 'affinity': [1244, 2341, 2417], '(ECM)': [1248], 'molecules,': [1249, 1343], 'being': [1250], 'deposited': [1251], 'ECM': [1254, 1342], 'after': [1255, 1347, 2242, 2385, 2873, 2962, 3083, 3986, 4028], 'synthesis': [1256], '(14Gao': [1257, 1354], 'Westling': [1259, 1356], 'Thompson': [1261, 1358], 'V.P.': [1262, 1359], 'Howell': [1263, 1360], 'T.D.': [1264, 1361], 'Gottschall': [1265, 1362], 'P.E.': [1266, 1363], '277:': [1273, 1325, 1370, 2594], '11034-11041Google': [1274, 1371], 'fact,': [1277], 'CR': [1283, 2052, 3928], 'and/or': [1284, 2568], 'sulfated': [1290], 'glycosaminoglycans': [1291], 'been': [1295], 'reported': [1296], '(15Flannery': [1297], 'Zeng': [1299], 'W.': [1300], 'Corcoran': [1301], 'Collins-Racie': [1303], 'L.A.': [1304], 'Chockalingam': [1305], 'P.S.': [1306], 'Hebert': [1307], 'Mackie': [1309], 'McDonagh': [1311], 'Crawford': [1313], 'Tomkinson': [1315], 'K.N.': [1316], 'LaVallie': [1317], 'E.R.': [1318], 'Morris': [1319], 'E.A.': [1320], '42775-42780Google': [1326], 'Interestingly,': [1328], 'full-length': [1333, 2218, 2253, 2676, 2799, 3060], 'active': [1334], 'blocked': [1337, 2654], 'probably': [1338], 'appears': [1346], 'removal': [1348], 'have': [1380, 4044, 4291], 'regulated.': [1388], 'information': [1391], 'available': [1393], 'interacting': [1396], 'study,': [1401], 'screening': [1403], 'library,': [1408], 'sought': [1410], 'regulating': [1418], 'Since': [1421, 3290], 'conserved': [1429], 'members,': [1432], 'candidate': [1450], 'regulator': [1453], 'study': [1461, 3222, 3718, 4278], 'inhibits': [1465], 'regions': [1477], 'each': [1479, 1797, 1848, 2146, 3684, 4004], 'molecule.': [1480], 'Yeast': [1481, 1610, 1686, 1860, 3559], 'Two-hybrid': [1482, 1687], 'System—MATCHMAKER': [1483], 'Gal4': [1484], '3': [1487, 2213, 2823, 3454], 'MATCHMAKER': [1490], 'purchased': [1496], 'Clontech': [1498], '(Palo': [1499], 'Alto,': [1500, 1865], 'CA).': [1501, 2097], 'amplified': [1512, 1562, 1700, 1766], 'PCR': [1514, 1563, 1702, 2076, 2103], 'set': [1517], 'primers': [1519], '(forward': [1520], 'primer,': [1521, 1525], '5′-GGAATTCCATATGAAGCAGTCAGGCTCCTTCAG-3′;': [1522], 'reverse': [1524], '5′-TTTGAATTCTTTCCTGCCCGCCCAGGG-3′)': [1526], 'expression': [1530, 1799, 2007], 'plasmid': [1531, 1795, 1800, 2008, 2183], 'pSG0688': [1532, 2012, 2078], 'described': [1534, 1858, 2475, 2807, 3093, 3288], 'previously': [1535, 3094], '(16Nakamura': [1536], 'Fujii': [1538, 2506, 2582], 'Inoki': [1540, 2580], 'Sugimoto': [1542, 3164], 'Matsuki': [1546], 'Miura': [1548], 'Yamaguchi': [1550], '275:': [1558], '38885-38890Google': [1559], 'product': [1564], 'corresponding': [1565], 'Lys687–Lys837': [1567], 'digested': [1569, 1769, 2014, 2085, 2140, 3270], 'NdeI': [1571], 'EcoRI': [1573, 1773, 2016, 2087, 2142], '(Takara': [1574], 'Bio': [1575], 'Inc.,': [1576, 1908, 2925], 'Otsu,': [1577], 'Japan)': [1578], 'into': [1581, 1594, 1657, 1788, 1803, 2029, 2092, 2156], 'pGBKT7': [1583], 'vector': [1584, 1791, 2971, 4221], '(Clontech),': [1585], 'generating': [1586], 'pGBKT7/TS4sp': [1588, 1794, 3566, 3593], 'plasmid.': [1589], 'plasmids': [1591, 1652, 2138], 'co-introduced': [1593, 1802], 'Saccharomyces': [1595], 'cerevisiae': [1596], 'strain': [1597, 1660, 1804, 3560], 'AH109': [1598, 3561], 'according': [1605, 1662, 1854, 2493, 2573, 3215, 3380], "manufacturer's": [1608, 1665, 3218], 'instructions.': [1609, 1666, 3219], 'transformants': [1611, 3652, 3672, 3696, 3704, 3708], 'plated': [1613], 'selected': [1615, 1809, 2206], 'medium': [1617, 1811, 1822, 2170, 3494, 3620], 'leucine,': [1619, 1815, 1825, 3497], 'tryptophan,': [1620, 1824, 3496], 'histidine,': [1621, 1826, 3498], 'adenine.': [1623, 3627], 'Robust': [1624], 'colonies': [1625, 1818, 3618], '>2': [1626], 'mm': [1627, 1944, 1961, 2278, 2283, 2351, 2359, 3090, 3344], 'diameter': [1629], 'restreaked': [1631], 'onto': [1632, 1821, 3131], 'same': [1634], 'agar': [1635], 'plates': [1636, 2606, 2645, 2724], 'allowed': [1638], 'grow': [1640, 3614], '1': [1642, 1943, 2382, 2393, 2743, 2850, 2894, 2938, 3343], 'week.': [1643], 'This': [1644], 'restreaking': [1645], 'step': [1646], 'repeated': [1648], 'twice': [1649, 2648, 2731, 2844], 'more,': [1650], 'isolated': [1654], 'introduced': [1656], 'Escherichia': [1658], 'coli': [1659], 'DH5α': [1661], 'Clones': [1667], 'harboring': [1668], 'target': [1669], 'isolated,': [1672], 'sequences': [1675], 'MegaBase': [1680], '1000': [1681, 3253, 3337, 4311], 'DNA': [1682], 'sequencer': [1683], '(Amersham': [1684, 2488, 3213], 'Biosciences).': [1685], 'Assay—cDNA': [1688], 'encoding': [1690, 2186, 3475, 3572], 'six': [1691], 'different': [1692, 3513], 'parts': [1693], '(see': [1696, 2069], 'Fig.': [1697, 2070, 3588, 3744, 3962, 4105, 4226, 4329], '1A)': [1698], 'template': [1710], 'following': [1713], 'primer': [1714, 2099, 2119, 2130], 'sets:': [1715], '5′-TTTGGATCCGTTATGACAATGGAAAACACTATC-3′': [1716], '(forward)': [1717, 1725, 1733, 1741, 1749, 1758], '5′-TTTGAATTCAGCTTGGATAGGTCTGTAAAG-3′': [1719], '(reverse)': [1720, 1728, 1736, 1744, 1752, 1761, 2112, 2122, 2133], 'I,': [1723, 3574], '5′-TTTGGATCCCAAGTGGTCCTGTCGAAGTA-3′': [1724], '5′-TTTGAATTCCAGTGTGGTAAAGACTCCAG-3′': [1727], 'II,': [1731, 3575], '5′-TTTGGATCCCTGGGAGCTCTATTCCACC-3′': [1732], '5′-TTTGAATTCAGTGATGGTGGCTCGAGGAG-3′': [1735], 'III,': [1739, 3576], '5′-TTTGGATCCCCCTCACCAACCTCACTCCA-3′': [1740], '5′-TTTGAATTCTTAATGGAAATTGGCTTGCT-3′': [1743], 'IV,': [1747, 3577], '5′-TTTGGATCCACCGAACAGAAATTGACAA-3′': [1748], '5′-TTTGAATTCCTGTGGACTGGGTTCCAATC-3′': [1751], 'V,': [1755, 3578], '5′-TTTGGATCCCTATTCCTGCACCAACTGAC-3′': [1757], '5′-TTTCTCGAGCTCTCGGGAATCTTCTCTGT-3′': [1760], 'VI.': [1764], 'products': [1767, 2083, 3113, 4015], 'BamHI': [1771, 1779], 'I–V,': [1776], 'XhoI': [1781, 2089], 'VI': [1784, 3580], 'then': [1786, 1808, 2479, 2672, 2716, 2881, 2912, 3115, 3269], 'pACT2': [1790], '(Clontech).': [1792], 'AH109.': [1805], 'They': [1806, 2670, 2943], 'tryptophan': [1813], 'raised': [1817], 'streaked': [1820], 'adenine': [1828], 'presence': [1831, 3341, 3800], '20': [1833, 2686, 3089], 'μg/ml': [1834, 2209, 2851], 'X-α-gal': [1835, 3664], 'cultured': [1837, 2165, 2201, 2882], '27': [1839], '°C': [1840, 1935, 3080, 3262], '2': [1842, 1949, 2203, 2665, 3258], 'days.': [1843], 'α-galactosidase': [1845, 3681], 'transformant': [1849, 3685], 'measured': [1851, 1870, 3371], 'p-nitrophenyl-α-d-galactoside': [1853], 'method': [1857, 2497, 3384], '(36Clontech': [1859], 'Protocols': [1861], 'Handbook.': [1862], 'Clontech,': [1863], 'Palo': [1864], 'CA2001Google': [1866], 'absorbance': [1873, 3691], '410': [1875, 3693], 'nm': [1876, 2636, 3073, 3231, 3254, 3338, 4312], 'normalized': [1878], 'density.': [1881], 'Chemical': [1882], 'Cross-linking—To': [1883], 'verify': [1884], 'fibronectin,': [1890, 2624, 3301, 3724, 3855, 4084], '125I-labeled': [1891, 2675, 2699, 2779, 3733, 3746, 3863, 4093, 4149], '(13': [1893], 'nm)': [1894, 3063], 'incubated': [1896, 2673, 2848, 2899, 2951, 3057, 3239, 4299], 'plasma': [1899], '(0,': [1901, 3245, 4303], '9.1,': [1902], '91': [1904], 'nm;': [1905, 1924, 2632], 'Chemicon': [1906], 'International,': [1907], 'Temecula,': [1909], 'CA)': [1910], '(Fn-f120;': [1922], '100': [1923, 1960], 'Invitrogen)': [1925], 'saline': [1928], '(PBS)': [1929], '16': [1931, 2639, 3076, 3277], 'h': [1932, 1950, 2640, 2666, 2690, 2939, 3077, 3259, 3278], '4': [1934, 2274, 2642, 2692], 'cross-linked': [1937, 3766, 3784, 3821], 'treating': [1939], 'mixture': [1941, 3779], 'suberate': [1946], '(DSS)': [1947], 'ice.': [1952], 'reaction': [1954, 3087, 3190, 3773], 'stopped': [1956], 'incubation': [1958, 2245, 2618, 2815, 3272], 'Tris-HCl': [1962, 2279, 2352], '(pH': [1963, 2280, 2353], '7.5)': [1964], '15': [1966], 'min': [1967, 2824, 2857], 'room': [1969, 2668, 2941], 'temperature,': [1970], 'sample': [1973], 'subjected': [1975, 2717, 2980, 3116], 'SDSPAGE': [1977], '(12.5%': [1978], 'total': [1979, 3120], 'acrylamide)': [1980, 3121], 'under': [1981, 2992, 3122], 'reducing': [1982, 3123], 'conditions.': [1983, 3124], 'gel': [1985, 2342, 3128], 'dried': [1987], 'analyzed': [1989, 3421], 'Fuji': [1993], 'Film': [1994], 'BAS': [1995], '2000': [1996], 'analyzer.': [1997], 'Construction': [1998, 2032], 'Its': [2002, 2604, 3028, 3907, 4276], 'Deletion': [2003], 'Mutants—To': [2004], 'construct': [2005], 'KpnI,': [2018, 2144], 'FLAG': [2024, 2152], 'tag': [2025, 2153], 'sequence': [2026], 'subcloned': [2028, 2155], 'pcDNA3.1/Zeo(–)': [2030, 2182, 2970], '(Invitrogen).': [2031], 'its': [2036, 2189, 2221, 2679, 3911], 'C-terminally': [2037, 3912, 4139], 'truncated': [2038, 2147, 2222, 2801, 3065, 3913, 4140], 'i.e.': [2040], '(Arg693–Lys837),': [2046], 'ΔCR/Sp': [2047, 2124, 2521, 4052], '(Pro603–Lys837),': [2058], 'ΔT/CR/Sp': [2060, 2135, 2331, 2371, 4058, 4098], '(Gly521–Lys837)': [2068], '2A),': [2071], 'carried': [2073, 3729], 'out': [2074, 3730], 'template.': [2081], 'Amplified': [2082], 'pCMVtag4a': [2093], '(Stratagene,': [2094], 'La': [2095], 'Jolla,': [2096], 'sets': [2100], 'follows:': [2106], '5′-TAATACGACTCACTATAGGG-3′': [2107], '(common': [2108], 'forward)': [2109], '5′-TTTCTCGAGGAAGGAGCCTGACTGCTTG-3′': [2111], '(Met1–Phe692),': [2115], 'common': [2117, 2128], 'forward': [2118, 2129], '5′-TTTCTCGAGCCCTGGGAAGCTCTTGA-3′': [2121], '(Met1–Gly602),': [2125], '5′-TTTCTCGAGAGCCTGTGGAATATTGAAG-3′': [2132], '(Met1–Ala520).': [2136], 'pcDNA3.1/Zeo(–).': [2157], 'Purification': [2158, 3900], 'Recombinant': [2160], 'Proteins—293T': [2162], '(DMEM)': [2171], '10%': [2173, 2835], 'fetal': [2174, 2836], 'transfected': [2178, 3947], 'above-mentioned': [2181], 'containing': [2184, 2328, 2381, 2392, 2868, 3570], 'DOTAP': [2192], 'liposomal': [2193], 'transfection': [2194], 'reagent': [2195], '(Roche': [2196], 'Diagnostics).': [2197], 'Transfected': [2198, 2795, 2966], 'days': [2204], '150': [2208], 'Zeocin': [2210], '(Invitrogen)': [2211, 2872], 'weeks.': [2214], 'Stable': [2215], 'established': [2225, 2805], 'expansion': [2227], 'single': [2230], 'cell,': [2231], 'conditioned': [2234, 2258, 3957], 'culture': [2235, 2424], 'media': [2236, 2259, 2425], 'harvested': [2241], '48-h': [2244], 'serum-free': [2247, 2866, 2876], 'DMEM.': [2248, 2877], 'For': [2249, 2404, 3220], 'purification': [2251, 2406], 'ΔT/CR/Sp,': [2256, 3918, 4219, 4260], 'concentrated': [2261, 2423], 'Amicon': [2264], 'Diaflo': [2265], 'apparatus': [2266], 'fitted': [2267], 'YM-10': [2270], 'membrane;': [2271], 'mixed': [2272, 2447, 2702], 'volumes': [2275, 2450], '50': [2277, 2350, 2620], '7.5),': [2281, 2354], '10': [2282, 2303, 2358, 2436, 2856], 'CaCl2,': [2284, 2360], '0.05%': [2285, 2361], 'Brij': [2286, 2362], '35,': [2287, 2363], '0.02%': [2289, 2365, 2820], 'NaN3': [2290, 2366], '(TCB': [2291], 'buffer);': [2292], 'applied': [2294, 2333, 2427, 2455, 2480], 'SP-Sepharose': [2297, 2458], 'fast': [2298, 2431, 2459, 2485], 'flow': [2299, 2432, 2460, 2486], 'column': [2300, 2336, 2388, 2433, 2487], '(2.5': [2301, 2434], '×': [2302, 2344, 2435, 2683, 2888, 3455], 'cm;': [2304, 2346, 2437], 'Amersham': [2305, 2438, 3197], 'Biosciences)': [2306, 2439, 2489, 3214], 'equilibrated': [2307, 2348, 2440], 'TCB': [2309, 2442, 2452], 'buffer.': [2310, 2536], 'recombinant': [2312, 2555, 3965, 4005, 4041, 4296], 'eluted': [2315, 2373, 2465, 2523], 'linear': [2318, 2468, 2526], 'gradient': [2319, 2469, 2527], '0–1': [2321, 2471, 2529], 'm': [2322, 2356, 2376, 2383, 2394, 2472, 2530], 'NaCl,': [2323, 2357], 'combined': [2326], 'fractions': [2327, 2446, 2752], 'anti-FLAG': [2338, 2420, 2915, 3995], 'antibody': [2339, 2421, 2916, 2919, 3153, 3195, 3996], 'M2': [2340], '(1': [2343, 2887], '6': [2345, 2375], 'Sigma)': [2347], '0.15': [2355], '(TNCB': [2367], 'buffer).': [2368], 'urea': [2377], 'TNCB': [2379, 2399, 2535, 2650, 2662, 2733], 'buffer': [2380, 2391, 2400, 2453, 2651, 2663, 2714], 'NaCl': [2384, 2395, 2473, 2531], 'washing': [2386, 2730, 2874, 2963], 'dialyzed': [2397, 2533], 'against': [2398, 2534], 'remove': [2402], 'urea.': [2403], 'ΔCR/Sp,': [2410, 3069, 3916, 3968, 4096, 4218, 4259], 'did': [2412], 'show': [2414], 'strong': [2416], 'gel,': [2422], 'DEAE-Sepharose': [2430], 'buffer,': [2443, 2734], 'unbound': [2445], '9': [2449], 'column.': [2461], 'above,': [2476], 'dialyzed,': [2477], 'chelating': [2483], 'Sepharose': [2484], 'charged': [2490], 'ZnCl2': [2492], 'our': [2495, 2575, 3382], 'previous': [2496, 2576, 3383], '(17Imai': [2498], 'Yokohama': [2500], 'Nakanishi': [2502], 'Ohuchi': [2504], 'Nakai': [2508], '6691-6697Google': [2517], 'concentrations': [2538, 3242, 3326], 'species': [2542, 2803, 3067, 3952, 4007, 4043, 4113, 4203], 'BCA': [2547], 'kit': [2550], '(Pierce).': [2551], 'purity': [2553], 'evaluated': [2558, 3139, 3687], 'SDS-PAGE,': [2560], 'followed': [2561], 'silver': [2563], 'staining': [2564, 3665], 'gels': [2567, 3985], 'autoradiography': [2569], 'iodinated': [2571], 'methods': [2577], '(18Hashimoto': [2578], 'Ikeda': [2586], '36288-36295Google': [2595], 'Binding': [2597, 3828, 4064], 'Immobilized': [2601], 'Fibronectin': [2602, 3542, 4274], 'Fragments—Microtiter': [2605], '96': [2608], 'wells': [2609, 2784, 3873], '(Immunomodule,': [2610], 'Nalge': [2611, 2891], 'Nunc,': [2612], 'Rochester,': [2613], 'NY)': [2614], 'coated': [2616, 2725], 'μl': [2621], 'intact': [2623, 2727, 3300, 3739, 3763, 3826, 3854, 3868, 4187, 4301], '(Fn-f40;': [2630], '25': [2631, 2635], 'Invitrogen),': [2633], 'Fn-f120': [2637, 3778], '°C.': [2643, 2693, 2827, 2860], 'washed': [2647, 2843], 'subsequently': [2653], '1%': [2656, 2869], 'albumin': [2659], '(BSA)': [2660], 'temperature.': [2669, 2942], '(∼5': [2682], '105': [2684, 2889], 'cpm,': [2685], 'ng/well)': [2687], '24': [2689], 'confirm': [2695], 'binding,': [2698], '10-': [2705], '50-fold': [2707], 'excess': [2708], 'amount': [2709, 3355, 3861], 'unlabeled': [2711, 4162], 'microtiter': [2723], 'After': [2729, 2828, 3189], 'bound': [2736, 2751, 2788, 3880], 'dissociated': [2739], 'treatment': [2741], 'n': [2744], 'NaOH,': [2745], 'radioactivity': [2748, 3419, 3879], 'counted': [2754], 'γ-counter': [2757], '(ARC-600,': [2758], 'Aloka,': [2759], 'Tokyo,': [2760], 'Japan).': [2761], 'Similarly,': [2762], 'possibility': [2764], 'examined': [2772, 3317, 3554, 4009, 4207], 'incubating': [2778, 3732, 4092], 'aggrecan-coated': [2783], 'measuring': [2786, 3689], 'radioactivity.': [2789], 'Laser': [2790], 'Scanning': [2791], 'Confocal': [2792], 'Microscopy': [2793], 'Cells—Stable': [2796], 'above': [2808], 'detached': [2810], 'dishes': [2813], '0.25%': [2817], 'trypsin': [2818, 2833], 'EDTA': [2821, 3091], '37': [2826, 2859, 3079, 3261, 3280], 'blocking': [2829], 'serum,': [2838], 'suspensions': [2841], 'PBS': [2846, 2854, 2906], 'suspended': [2864], 'DMEM': [2867], 'insulin/transferrin/selenium/X': [2870], 'supplement': [2871], 'Fibronectin-treated': [2878], 'Lab-Tek': [2884], 'chamber': [2885], 'slides': [2886], 'cells/well;': [2890], 'Nunc)': [2892], 'day.': [2895], '3%': [2901], 'normal': [2902], 'goat': [2903], 'block': [2908, 3352], 'nonspecific': [2909], 'reacted': [2913, 3761], '(1:100),': [2917], 'anti-fibronectin': [2918], '(1:': [2920], '20;': [2921], 'Santa': [2922, 2926], 'Cruz': [2923], 'Biotechnology': [2924], 'Cruz,': [2927], 'CA),': [2928], 'non-immune': [2930], 'mouse': [2931], 'IgG': [2932], '(Dako': [2933], 'Corp.,': [2934], 'Glostrup,': [2935], 'Denmark)': [2936], 'fixed': [2945], 'methanol/acetone/formaldehyde': [2947], '(19:19:2,': [2948], 'v/v)': [2949], 'fluorescein': [2953, 3006], 'isothiocyanate-': [2954], 'rhodamine-conjugated': [2956], 'secondary': [2957], 'antibodies': [2958], '(1:50;': [2959], 'Dako': [2960], 'Corp.)': [2961], 'PBS.': [2965], '(mock': [2972], 'transfectants)': [2973], 'parental': [2975], 'double': [2982, 4209], 'immunostaining': [2983, 4210], 'negative': [2986], 'control.': [2987], 'All': [2988, 3988], 'preparations': [2989, 3361], 'viewed': [2991, 3020], 'Olympus': [2994], 'laser': [2995], 'scanning': [2996, 3373], 'microscope': [2998], 'similar': [3001], 'sensitivity': [3002], '(550': [3003], 'V': [3004, 3010], 'isothiocyanate': [3007], '600': [3009], 'rhodamine),': [3012], 'differential': [3014], 'interference': [3015], 'contrast': [3016, 3629], 'images': [3017], 'comparison.': [3022], 'Detection': [3023], 'Aggrecanase': [3025, 3902, 4269], 'Activity': [3026, 3903, 4270], 'Inhibition': [3029, 4266], 'Fibronectin—': [3031], 'Aggrecan': [3032, 3267], '(100': [3033], 'μg)': [3034], 'prepared': [3035], 'nasal': [3038], '(12Hashimoto': [3040, 3095, 3385], 'purified': [3059, 3954, 3964], '(8': [3062, 3230], '(ΔSp,': [3068, 3915], 'ΔT/CR/Sp;': [3071], '8': [3072], 'each)': [3074], 'deglycosylated': [3082], 'termination': [3084], 'digestion': [3112, 4014, 4029], 'SDS-PAGE': [3118], '(10%': [3119], 'Proteins': [3125, 3441], 'transferred': [3130], 'polyvinylidene': [3132], 'fluoride': [3133], 'membranes,': [3134], 'detecting': [3141], 'neoepitope': [3146], '(NITEGE373)': [3147], 'immunoblotting': [3149, 3993, 4011, 4321], 'neoepitope-specific': [3152, 4018, 4324], 'I19C': [3154], '(2': [3155], 'μg/ml),': [3156], 'kindly': [3159], 'provided': [3160], 'Drs.': [3162], 'Kotaro': [3163], 'Kazuhiko': [3166], '(Sankyo': [3168], 'Co.,': [3169], 'Ltd.,': [3170], 'Tokyo)': [3171], '(19Sugimoto': [3172], 'Takahashi': [3174], 'Yamamoto': [3176], 'Shimada': [3178], '(Tokyo).': [3184], '126:': [3186], '449-455Google': [3187], 'horseradish': [3192], 'peroxidase-linked': [3193], 'anti-IgG': [3194], '(1:5000;': [3196], 'Biosciences),': [3198], 'immunoreactive': [3199, 3368, 4021], 'membranes': [3203], 'detected': [3205], 'ECL™': [3208], 'Western': [3209], 'blot': [3210], 'detection': [3211], 'inhibition,': [3225], 'each),': [3232], 'sufficient': [3235], 'activity,': [3237, 4056, 4294], 'increasing': [3241], '10,': [3246, 3329, 4304], '20,': [3247, 3330, 4305], '40,': [3248, 3331, 4306], '100,': [3249, 3332, 4307], '200,': [3250, 3333, 4308], '500,': [3251, 3334, 4309], 'final': [3255, 3325, 4313], 'concentrations)': [3256], 'prior': [3263], 'reaction.': [3266], 'mixtures': [3275], '°C,': [3281], 'monitored': [3286], 'above.': [3289], 'inhibitory': [3303], 'effects': [3304], '(Fn-f40': [3309], 'Fn-f120)': [3311, 3885], 'preincubated': [3320], '0,': [3328], '750,': [3335], 'phenylmethanesulfonyl': [3345], 'fluoride,': [3346], 'added': [3349], 'completely': [3351], 'trace': [3354], 'serine': [3357], 'proteinase(s)': [3358], 'contaminating': [3359], 'fragments.': [3364], 'densities': [3366], 'bands': [3369, 3974, 3989], 'densitometry': [3374], 'NIH': [3376], 'Image': [3377], 'Version': [3378, 3431], '1.62': [3379], 'Statistical': [3401], 'Analysis—Measured': [3402], 'values': [3403], 'mean': [3408], '±': [3409], 'S.D.': [3410], 'assay,': [3415], 'difference': [3417], 'Bonferroni/Dunn': [3424], 'test.': [3425], 'Tests': [3426], 'performed': [3428, 3851, 4090], 'StatView': [3430], '5.0.': [3432], 'p': [3433], '<': [3434, 3894], '0.05': [3435], 'significant.': [3438], 'Screening': [3439], 'That': [3442], 'Interact': [3443], '—To': [3446], 'identify': [3447, 3837], 'interact': [3451], '106': [3456], '(Lys687–Lys837,': [3480], '151': [3481], 'amino': [3482, 3624], 'acids)': [3483], 'bait.': [3487], '156': [3490], 'grown': [3492], 'adenine,': [3500], '31': [3501, 3510], '(20%)': [3503], 'identified': [3505], 'had': [3512, 4059, 4121], '5′-ends,': [3514], 'but': [3515], 'them': [3518], 'except': [3519], 'shortest': [3522], 'covered': [3524], 'whole': [3526, 3930], 'region': [3527, 3546, 3839], 'heparin-binding': [3531], 'domain/fibrin-binding': [3532], '(Fig.': [3536, 3583, 3646, 3677, 3709, 3898, 3941, 3997, 4035, 4062, 4143, 4168, 4264], '1A).': [3537, 3584], 'Fragments': [3543], 'Yeast—The': [3545], 'assays.': [3558], 'cotransformed': [3563, 3591], 'pACT2/FnI–VI': [3568], 'plasmids,': [3569], '1B,': [3589], 'pACT2/FnVI': [3595], '(referred': [3596, 3609], 'Sp/VI)': [3599], 'well': [3601], 'positive': [3603, 3649], 'p53': [3606], 'SV40': [3608], 'p53/SV40)': [3612], 'could': [3613, 3865], 'form': [3616], 'blue-stained': [3617], 'three': [3623], 'acids': [3625], 'transformants,': [3632, 3657], 'other': [3633, 3703, 3771, 4050], 'I–V': [3640], 'background': [3644, 4123], '1B).': [3647, 3678], 'Although': [3648, 3786, 4239], 'control': [3650, 3676, 3706], 'p53/SV40': [3651, 3707], 'grew': [3653], 'faster': [3654], 'than': [3655, 3673, 3889, 4116, 4136], 'Sp/VI': [3656, 3671, 3695], 'intensity': [3659], 'blue': [3661], 'color': [3662], 'much': [3667], 'higher': [3668, 3699, 3888, 4115, 4135], 'When': [3679, 3999], 'nm,': [3694], 'remarkably': [3698, 4133], 'compared': [3701], '1C).': [3710], 'Cross-linking': [3711], 'Study': [3712], 'Fibronectin—To': [3716, 3836, 4073], 'cross-linking': [3726], 'experiment': [3727], 'increased': [3736], 'amounts': [3737], '1D,': [3745], 'migrated': [3749], '73': [3751], 'kDa,': [3752, 3981], 'shifted': [3753], 'site': [3756], '∼400': [3758], 'kDa': [3759, 3797, 4026], 'when': [3760], 'DSS.': [3768], 'On': [3769, 4048], 'hand,': [3772, 4051], 'DSS': [3781], 'products.': [3785], 'became': [3788], 'broad': [3790], 'band': [3791], 'ranging': [3792], '70': [3794], '80': [3796], 'dimerization': [3803], 'proteinase': [3806], 'detected.': [3809], 'molecular': [3812], 'shift': [3813], 'caused': [3818], 'complex': [3822], 'Region': [3834], 'interacts': [3843], 'immobilized': [3853], 'Fn-f40.': [3858], 'large': [3860], 'fibronectin-coated': [3869, 4100], 'wells,': [3870], 'BSA-coated': [3872], 'back-ground': [3876], 'binding.': [3877], 'Fn-f40': [3882], '(but': [3883], 'significantly': [3887], 'BSA': [3892], '(p': [3893], '0.01': [3895], 'versus': [3896], 'BSA)': [3897], '1E).': [3899], 'Mutants—Full-length': [3908], 'lack': [3920], 'domain;': [3924, 3932], 'domains,': [3939], 'respectively)': [3940], '2A)': [3942], 'stably': [3946], 'cells.': [3949], 'media.': [3958], '2B,': [3963], 'ΔSp,': [3967, 4095, 4217, 4258, 4287], 'ΔT/CT/Sp': [3970], '73,': [3976], '58,': [3977], '48,': [3978], '39': [3980], 'respectively,': [3982], 'silver-stained': [3984], 'SDS-PAGE.': [3987], 'recognized': [3991], '2C).': [3998], 'antibody,': [4019], '∼80': [4025], 'appeared': [4027], '2D),': [4036], 'indicating': [4037], 'two': [4040], 'potent': [4045], 'weak': [4055], '2D).': [4063], 'Spacer': [4068], 'Domain': [4069], 'determine': [4074], 'wells.': [4101], '3A,': [4106], 'signals.': [4124], '∼3-fold': [4134], '3A).': [4144], 'confirmed': [4154], 'since': [4155], 'competitively': [4159], 'dose-dependent': [4166], 'manner': [4167], '3B).': [4169], 'results': [4172], '(Arg693–Lys837)': [4179], 'Docking': [4189], 'Cell': [4194], 'Membrane': [4195], 'Fibronectin-coated': [4197], 'Cells—The': [4198], 'pericellular': [4199], 'alone.': [4222], '4,': [4227], 'immunolocalized': [4230], 'transfectants.': [4238], 'strongly': [4243], 'colocalized': [4244], 'surface,': [4250], 'immunoreaction': [4254], 'mock': [4262], '4).': [4265], 'Fragments—To': [4277], 'effect': [4280], 'both': [4288], 'definite': [4292], 'proteinases': [4297], 'concentrations),': [4314], 'antibody.': [4325], '5A,': [4330], 'c': [4332]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2109697092', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}, {'year': 2021, 'cited_by_count': 3}, {'year': 2020, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 3}, {'year': 2017, 'cited_by_count': 1}, {'year': 2016, 'cited_by_count': 1}, {'year': 2015, 'cited_by_count': 5}, {'year': 2013, 'cited_by_count': 1}, {'year': 2012, 'cited_by_count': 6}], 'updated_date': '2024-12-14T20:24:20.505294', 'created_date': '2016-06-24'}