Title: Death-associated protein kinase (DAPK1) in cerebral cortex of late-onset Alzheimer's disease patients and aged controls
Abstract: Neuropathology and Applied NeurobiologyVolume 36, Issue 1 p. 17-24 Death-associated protein kinase (DAPK1) in cerebral cortex of late-onset Alzheimer's disease patients and aged controls A. H. Hainsworth, Corresponding Author A. H. Hainsworth Clinical Neuroscience, St George's University of London, London, De Montfort University, Leicester,Atticus H. Hainsworth, Centre for Clinical Neuroscience (mailpoint J-0B), St George's University of London, Cranmer Terrace, London SW17 0RE, UK. Tel: +44 208 725 5586; Fax: +44 208 725 2950; E-mail: [email protected]Search for more papers by this authorR. C. Allsopp, R. C. Allsopp De Montfort University, Leicester, Present address: Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, UK.Search for more papers by this authorA. Jim, A. Jim St George's Healthcare NHS Trust, London,Search for more papers by this authorJ. F. Potter, J. F. Potter University of East Anglia Medical School, Norwich,Search for more papers by this authorJ. Lowe, J. Lowe University of East Anglia Medical School, Norwich,Search for more papers by this authorC. J. Talbot, C. J. Talbot School of Molecular Medical Sciences, University of Nottingham Medical School, Nottingham,Search for more papers by this authorR. J. Prettyman, R. J. Prettyman Institute of Genetics, University of Leicester, Leicester, andSearch for more papers by this author A. H. Hainsworth, Corresponding Author A. H. Hainsworth Clinical Neuroscience, St George's University of London, London, De Montfort University, Leicester,Atticus H. Hainsworth, Centre for Clinical Neuroscience (mailpoint J-0B), St George's University of London, Cranmer Terrace, London SW17 0RE, UK. Tel: +44 208 725 5586; Fax: +44 208 725 2950; E-mail: [email protected]Search for more papers by this authorR. C. Allsopp, R. C. Allsopp De Montfort University, Leicester, Present address: Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, UK.Search for more papers by this authorA. Jim, A. Jim St George's Healthcare NHS Trust, London,Search for more papers by this authorJ. F. Potter, J. F. Potter University of East Anglia Medical School, Norwich,Search for more papers by this authorJ. Lowe, J. Lowe University of East Anglia Medical School, Norwich,Search for more papers by this authorC. J. Talbot, C. J. Talbot School of Molecular Medical Sciences, University of Nottingham Medical School, Nottingham,Search for more papers by this authorR. J. Prettyman, R. J. Prettyman Institute of Genetics, University of Leicester, Leicester, andSearch for more papers by this author First published: 07 January 2010 https://doi.org/10.1111/j.1365-2990.2009.01035.xCitations: 13 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract A. H. Hainsworth, R. C. Allsopp, A. Jim, J. F. Potter, J. Lowe, C. J. Talbot and R. J. Prettyman (2010) Neuropathology and Applied Neurobiology36, 17–24Death-associated protein kinase (DAPK1) in cerebral cortex of late-onset Alzheimer's disease patients and aged controls Aims: Here our objective was to detect the pro-apoptotic serine/threonine kinase death-associated protein kinase (DAPK1) in aged human cerebral cortex and to test the hypothesis that DAPK1 abundance is associated with late-onset Alzheimer's disease (AD). Methods: Using Western analysis and immunohistochemistry we evaluated post mortem frontal cerebral cortex from patients with severe AD (mean age 76 years, range 66–91, n = 11, all male), and from control cases without serious central nervous system illness (mean age 77 years, range 61–95, n = 12, all male). We also examined brains of Tg2576 transgenic mice (males, aged 16–21 months), a model for chronic amyloid-induced brain injury. Results: Immunohistochemical labelling showed DAPK1 expression in cortical neurones of human cortex and axonal tracts within subcortical white matter, both in AD and in control brains. Western analysis confirmed DAPK1 expression in all samples, although expression was very low in some control cases. DAPK1 abundance in the AD group was not significantly different from that in controls (P = 0.07, Mann–Whitney test). In brains of Tg2576 mice DAPK1 abundance was very similar to that in wild-type littermates (P = 0.96, Mann–Whitney test). Conclusion: We found that DAPK1 was expressed in neurones of aged human frontal cortex, both in AD and in control cases. References 1 Bialik S, Kimchi A. The death-associated protein kinases: structure, function, and beyond. Annu Rev Biochem 2006; 75: 189– 210 2 Stevens C, Hupp TR. Novel insights into DAPK autophagic signalling using peptide aptamer combinatorial protein-interaction screens. 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Publication Year: 2009
Publication Date: 2009-07-14
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 19
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