Title: RNA‐Directed Packaging of Enzymes within Virus‐like Particles
Abstract: Angewandte Chemie International EditionVolume 49, Issue 50 p. 9648-9651 Communication RNA-Directed Packaging of Enzymes within Virus-like Particles† Jason D. Fiedler, Jason D. Fiedler Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-8850Search for more papers by this authorSteven D. Brown, Steven D. Brown Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-8850Search for more papers by this authorJolene L. Lau, Jolene L. Lau Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-8850Search for more papers by this authorProf. M. G. Finn, Corresponding Author Prof. M. G. Finn [email protected] Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-8850Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-8850Search for more papers by this author Jason D. Fiedler, Jason D. Fiedler Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-8850Search for more papers by this authorSteven D. Brown, Steven D. Brown Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-8850Search for more papers by this authorJolene L. Lau, Jolene L. Lau Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-8850Search for more papers by this authorProf. M. G. Finn, Corresponding Author Prof. M. G. Finn [email protected] Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-8850Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-8850Search for more papers by this author First published: 09 November 2010 https://doi.org/10.1002/anie.201005243Citations: 171 † We are grateful to Dr. So-Hye Cho for assistance in acquiring TEM images, and to Dr. Joshua Price for analytical ultracentrifugation. The pRevTRE-Luc vector was a kind gift from Ashley Pratt. This project was supported by the National Institutes of Health (CA112075 and RR021886), the Skaggs Institute for Chemical Biology, and the W.M. Keck Foundation. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Graphical Abstract Packaged molecular machines are available using dual expression vectors that guide the preparation of Qβ virus-like particles encapsulating multiple enzymes. Packaging is promoted by RNA aptamer sequences that bridge between the coat protein and a peptide tag fused to the desired cargo (see scheme). Peptidase E and luciferase were thus encapsulated and shown to be catalytically active inside the particle. Supporting Information Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Filename Description anie_201005243_sm_miscellaneous_information.pdf1.3 MB miscellaneous_information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. References 1C. A. Kerfeld, M. R. Sawaya, S. Tanaka, C. V. Nguyen, M. Phillips, M. Beeby, T. O. Yeates, Science 2005, 309, 936. 2 2aW. Tischer, F. Wedekind, Top. Curr. 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