Title: Fully automated simple analytical peptide chromatography on the amino acid analyzer at the 10−8 mole level
Abstract: Automated peptide analysis has been investigated under a variety of conditions in order to determine those most suitable for short analysis time, optimum resolution, low sample loads, and little or no modification to the analyzer. This has been achieved on 13.5–14 × 0.9 cm columns of Aminex A5 with an elution rate for buffer and ninhydrin each at 100 ml/hr. Chromatography was at 55° and total analyses were complete in 6–7 hr. Other than the slightly longer column to be used for basic amino acids, no other modifications to the analyzer were necessary. Sample loads of peptides from 0.5–1 mg of original protein gave excellent color yields, and the operation may be run routinely with peptides from 0.5 mg of protein. Examples of the utility of the system are shown in the analysis of tryptic peptides of reduced-carboxymethylated lysozyme and of apomyoglobin.
Publication Year: 1972
Publication Date: 1972-09-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 2
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