Title: Haploid regeneration aptitude of maize (Zea mays L.) lines of various origin and of their hybrids
Abstract: One maize line with good haploid regenera- tion aptitude and one recalcitrant in this respect, together with various hybrid combinations of these, were used to develop new doubled haploid (DH) lines. A line originat- ing from an exotic Chinese stock was crossed with a re- calcitrant Martonvasar line, used as a parental component in several commercial hybrids, to determine the anther re- sponse, callus induction and plant regeneration of the reciprocal hybrids and the fertility of the adult plants. The same tests were also made on previously developed DH lines of these reciprocal hybrids and on DH lines ob- tained after a single backcross with the recalcitrant par- ent. The results indicated that haploid regeneration apti- tude was primarily an inherited trait in the experimental material, with a heterosis effect in the F 1 progeny. The hybrids exhibited no statistically significant reciprocal ef- fect. The anther response, callus induction and plant re- generation of the DH lines developed from the hybrids and the fertility of regenerated plants were comparable to the initial combinations even when the DH line originated from a hybrid developed by backcrossing with the recal- citrant line. Data are also reported on the anther response and haploid regeneration frequency of genotypes known to have excellent tissue culturing aptitude from immature zygotic embryos. The present results may contribute to the development of an optimum gene ratio where, in ad- dition to maintaining haploid regeneration aptitude, the DH lines also possess important agronomic traits, which are then reliably inherited.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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Cited By Count: 5
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