Publication Information

{'id': 'https://openalex.org/W2103190727', 'doi': 'https://doi.org/10.1194/jlr.m034678', 'title': 'Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids', 'display_name': 'Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids', 'publication_year': 2013, 'publication_date': '2013-01-17', 'ids': {'openalex': 'https://openalex.org/W2103190727', 'doi': 'https://doi.org/10.1194/jlr.m034678', 'mag': '2103190727', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/23325927', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/3617957'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1194/jlr.m034678', 'pdf_url': 'http://www.jlr.org/content/54/3/831.full.pdf', 'source': {'id': 'https://openalex.org/S11400418', 'display_name': 'Journal of Lipid Research', 'issn_l': '0022-2275', 'issn': ['0022-2275', '1539-7262'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'doaj', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'hybrid', 'oa_url': 'http://www.jlr.org/content/54/3/831.full.pdf', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5023419556', 'display_name': 'Keisuke Obara', 'orcid': 'https://orcid.org/0000-0002-2441-0907'}, 'institutions': [{'id': 'https://openalex.org/I205349734', 'display_name': 'Hokkaido University', 'ror': 'https://ror.org/02e16g702', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I205349734']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Keisuke Obara', 'raw_affiliation_strings': ['Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812 (Japan).'], 'affiliations': [{'raw_affiliation_string': 'Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812 (Japan).', 'institution_ids': ['https://openalex.org/I205349734']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5104753887', 'display_name': 'Ryo Kojima', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I205349734', 'display_name': 'Hokkaido University', 'ror': 'https://ror.org/02e16g702', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I205349734']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Ryo Kojima', 'raw_affiliation_strings': ['Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812 (Japan).'], 'affiliations': [{'raw_affiliation_string': 'Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812 (Japan).', 'institution_ids': ['https://openalex.org/I205349734']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5017760891', 'display_name': 'Akio Kihara', 'orcid': 'https://orcid.org/0000-0001-5889-0788'}, 'institutions': [{'id': 'https://openalex.org/I205349734', 'display_name': 'Hokkaido University', 'ror': 'https://ror.org/02e16g702', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I205349734']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Akio Kihara', 'raw_affiliation_strings': ['Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812 (Japan).'], 'affiliations': [{'raw_affiliation_string': 'Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812 (Japan).', 'institution_ids': ['https://openalex.org/I205349734']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'apc_paid': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'fwci': 2.09, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 28, 'citation_normalized_percentile': {'value': 0.747741, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 92, 'max': 93}, 'biblio': {'volume': '54', 'issue': '3', 'first_page': '831', 'last_page': '842'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11150', 'display_name': 'Endoplasmic Reticulum Stress and Disease', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11150', 'display_name': 'Endoplasmic Reticulum Stress and Disease', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10617', 'display_name': 'Cellular transport and secretion', 'score': 0.9998, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/vesicular-transport-protein', 'display_name': 'Vesicular Transport Proteins', 'score': 0.49660832}], 'concepts': [{'id': 'https://openalex.org/C102747710', 'wikidata': 'https://www.wikidata.org/wiki/Q824237', 'display_name': 'Endosome', 'level': 3, 'score': 0.7659559}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.5629106}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.5344877}, {'id': 'https://openalex.org/C167019083', 'wikidata': 'https://www.wikidata.org/wiki/Q4082043', 'display_name': 'Vesicular transport protein', 'level': 4, 'score': 0.49660832}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.46278602}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.3220315}, {'id': 'https://openalex.org/C130316041', 'wikidata': 'https://www.wikidata.org/wiki/Q189206', 'display_name': 'Vesicle', 'level': 3, 'score': 0.25847185}, {'id': 'https://openalex.org/C1491633281', 'wikidata': 'https://www.wikidata.org/wiki/Q7868', 'display_name': 'Cell', 'level': 2, 'score': 0.1340867}, {'id': 'https://openalex.org/C41625074', 'wikidata': 'https://www.wikidata.org/wiki/Q176088', 'display_name': 'Membrane', 'level': 2, 'score': 0.13341454}], 'mesh': [{'descriptor_ui': 'D011992', 'descriptor_name': 'Endosomes', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D005227', 'descriptor_name': 'Fatty Acids', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D000123', 'descriptor_name': 'Acetyltransferases', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D000123', 'descriptor_name': 'Acetyltransferases', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D043402', 'descriptor_name': 'Cathepsin A', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D043402', 'descriptor_name': 'Cathepsin A', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D043402', 'descriptor_name': 'Cathepsin A', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D011992', 'descriptor_name': 'Endosomes', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005227', 'descriptor_name': 'Fatty Acids', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D029701', 'descriptor_name': 'Saccharomyces cerevisiae Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D029701', 'descriptor_name': 'Saccharomyces cerevisiae Proteins', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D033921', 'descriptor_name': 'Vesicular Transport Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D033921', 'descriptor_name': 'Vesicular Transport Proteins', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D020691', 'descriptor_name': 'rab GTP-Binding Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D020691', 'descriptor_name': 'rab GTP-Binding Proteins', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}], 'locations_count': 6, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1194/jlr.m034678', 'pdf_url': 'http://www.jlr.org/content/54/3/831.full.pdf', 'source': {'id': 'https://openalex.org/S11400418', 'display_name': 'Journal of Lipid Research', 'issn_l': '0022-2275', 'issn': ['0022-2275', '1539-7262'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://doaj.org/article/a60b16bb25e84a328fc1ab551ff5768a', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306401280', 'display_name': 'DOAJ (DOAJ: Directory of Open Access Journals)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': None, 'host_organization_name': None, 'host_organization_lineage': [], 'host_organization_lineage_names': [], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': True, 'landing_page_url': 'https://europepmc.org/articles/pmc3617957', 'pdf_url': 'https://europepmc.org/articles/pmc3617957?pdf=render', 'source': {'id': 'https://openalex.org/S4306400806', 'display_name': 'Europe PMC (PubMed Central)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1303153112', 'host_organization_name': 'European Bioinformatics Institute', 'host_organization_lineage': ['https://openalex.org/I1303153112'], 'host_organization_lineage_names': ['European Bioinformatics Institute'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'http://hdl.handle.net/2115/52255', 'pdf_url': 'https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/52255/1/JLR54-3_831-842.pdf', 'source': {'id': 'https://openalex.org/S4306400549', 'display_name': 'Hokkaido University Collection of Scholarly and Academic Papers (Hokkaido University)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I205349734', 'host_organization_name': 'Hokkaido University', 'host_organization_lineage': ['https://openalex.org/I205349734'], 'host_organization_lineage_names': ['Hokkaido University'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617957', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/23325927', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1194/jlr.m034678', 'pdf_url': 'http://www.jlr.org/content/54/3/831.full.pdf', 'source': {'id': 'https://openalex.org/S11400418', 'display_name': 'Journal of Lipid Research', 'issn_l': '0022-2275', 'issn': ['0022-2275', '1539-7262'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 47, 'referenced_works': ['https://openalex.org/W1631725776', 'https://openalex.org/W1654890776', 'https://openalex.org/W1973707729', 'https://openalex.org/W1975430023', 'https://openalex.org/W1981693484', 'https://openalex.org/W1984382879', 'https://openalex.org/W1984954439', 'https://openalex.org/W1996252412', 'https://openalex.org/W1999919904', 'https://openalex.org/W2008811583', 'https://openalex.org/W2008943739', 'https://openalex.org/W2014581910', 'https://openalex.org/W2016878931', 'https://openalex.org/W2017849731', 'https://openalex.org/W2020989183', 'https://openalex.org/W2021032365', 'https://openalex.org/W2049260737', 'https://openalex.org/W2050152694', 'https://openalex.org/W2057704289', 'https://openalex.org/W2066160116', 'https://openalex.org/W2067253037', 'https://openalex.org/W2072070715', 'https://openalex.org/W2076260974', 'https://openalex.org/W2077179584', 'https://openalex.org/W2079368124', 'https://openalex.org/W2097666362', 'https://openalex.org/W2110522042', 'https://openalex.org/W2117864629', 'https://openalex.org/W2120328280', 'https://openalex.org/W2123363603', 'https://openalex.org/W2130071037', 'https://openalex.org/W2130905895', 'https://openalex.org/W2137994863', 'https://openalex.org/W2140843995', 'https://openalex.org/W2140948740', 'https://openalex.org/W2141488967', 'https://openalex.org/W2146503498', 'https://openalex.org/W2148053098', 'https://openalex.org/W2149797789', 'https://openalex.org/W2151027516', 'https://openalex.org/W2151472754', 'https://openalex.org/W2160239356', 'https://openalex.org/W2164236208', 'https://openalex.org/W2168295234', 'https://openalex.org/W2169333620', 'https://openalex.org/W2170678054', 'https://openalex.org/W317324549'], 'related_works': ['https://openalex.org/W4229496802', 'https://openalex.org/W3134608159', 'https://openalex.org/W2155175976', 'https://openalex.org/W2149342305', 'https://openalex.org/W2132576112', 'https://openalex.org/W2116012460', 'https://openalex.org/W2077683769', 'https://openalex.org/W1997113151', 'https://openalex.org/W1979102242', 'https://openalex.org/W1480317432'], 'abstract_inverted_index': {'Very': [0, 177, 503, 554], 'long-chain': [1, 178, 389, 445, 504, 555, 579, 838, 847, 1077, 1155, 2724, 4068], 'fatty': [2, 5, 179, 182, 390, 505, 556, 721, 766, 974, 1042, 1078, 1645, 1700, 1751, 4069], 'acids': [3, 6, 180, 183, 393, 722, 1752, 2100], '(VLCFAs),': [4, 181], 'with': [7, 129, 184, 306, 435, 612, 717, 896, 1375, 1747, 1957, 1984, 2105, 2138, 2687, 2945, 3080, 3223, 3345, 3359, 3392, 3468, 3516, 3600, 3685, 3701, 3733, 3755, 3762, 3767, 3783, 3814, 3822, 3849, 3888, 3980, 4021, 4180, 4197, 4220], 'chain-length': [8, 185, 438, 615, 927, 4131, 4199], 'greater': [9, 186, 439], 'than': [10, 187, 440, 932], '20': [11, 188, 441, 3219, 3374, 3652], 'carbons,': [12, 189], 'possess': [13, 190], 'a': [14, 54, 59, 68, 77, 80, 123, 136, 191, 231, 236, 245, 254, 257, 300, 313, 609, 613, 805, 889, 926, 1123, 1154, 1220, 1228, 1278, 1301, 1314, 1353, 1513, 1526, 1625, 1630, 1671, 1817, 1933, 1950, 1954, 1963, 2139, 2173, 2678, 2688, 2716, 2799, 3246, 3473, 3490, 3500, 3628, 3648, 3800, 3832, 3859, 3886, 3902, 3924, 4062, 4104, 4156, 4198, 4217], 'wide': [15, 192], 'range': [16, 193], 'of': [17, 43, 71, 132, 138, 161, 168, 194, 220, 248, 309, 315, 338, 345, 397, 426, 432, 437, 457, 471, 529, 533, 547, 616, 648, 675, 697, 715, 730, 740, 763, 772, 804, 830, 846, 894, 903, 910, 918, 928, 966, 1004, 1019, 1034, 1122, 1153, 1171, 1198, 1219, 1235, 1259, 1307, 1317, 1415, 1483, 1519, 1529, 1561, 1590, 1642, 1651, 1673, 1697, 1706, 1745, 1760, 1770, 1793, 1805, 1814, 1838, 1860, 1875, 1914, 1953, 1962, 1999, 2134, 2176, 2278, 2320, 2362, 2404, 2446, 2488, 2530, 2572, 2614, 2656, 2715, 2744, 2782, 2787, 2901, 2914, 2963, 3021, 3037, 3073, 3082, 3092, 3110, 3131, 3142, 3188, 3282, 3315, 3324, 3329, 3355, 3428, 3622, 3631, 3644, 3651, 3725, 3736, 3744, 3803, 3874, 3894, 3905, 3923, 3952, 3973, 3983, 3996, 4008, 4017, 4034, 4040, 4060, 4132, 4146, 4187, 4200], 'biological': [18, 195, 1196], 'functions.': [19, 196, 1942], 'However,': [20, 197, 1868, 2032], 'their': [21, 198, 408, 869, 1897, 3881], 'roles': [22, 199, 540, 872, 3882], 'at': [23, 200, 2035, 2759, 2784, 2885, 2981, 3005, 3085, 3336, 3581, 3612, 3753, 3958, 3966, 3969, 4093, 4095, 4108, 4122, 4210], 'the': [24, 31, 50, 62, 72, 93, 107, 112, 118, 130, 139, 152, 163, 174, 201, 208, 227, 239, 249, 270, 284, 289, 295, 307, 316, 329, 340, 351, 472, 527, 605, 649, 695, 703, 737, 770, 844, 904, 933, 941, 967, 1002, 1005, 1017, 1035, 1147, 1308, 1321, 1346, 1398, 1417, 1424, 1520, 1533, 1563, 1570, 1593, 1607, 1649, 1687, 1704, 1726, 1734, 1767, 1791, 1797, 1802, 1806, 1822, 1843, 1878, 1887, 1926, 1945, 1979, 1987, 2002, 2036, 2041, 2049, 2057, 2130, 2135, 2279, 2321, 2363, 2405, 2447, 2489, 2531, 2573, 2615, 2657, 2699, 2765, 2775, 2788, 2793, 2814, 2818, 2822, 2828, 2846, 2850, 2854, 2886, 2898, 2902, 2918, 2937, 2942, 2946, 2951, 2960, 2964, 2982, 3006, 3018, 3022, 3034, 3053, 3086, 3100, 3111, 3117, 3154, 3252, 3363, 3397, 3412, 3432, 3694, 3872, 3906, 3950, 3971, 3974, 3981, 3994, 3997, 4005, 4009, 4018, 4022, 4028, 4038, 4114, 4130, 4133, 4136, 4143, 4147, 4170], 'molecular': [25, 202, 1071], 'level': [26, 203], 'remain': [27, 204], 'largely': [28, 205, 4178], 'unknown.': [29, 206], 'In': [30, 116, 207, 293, 448, 603, 1145, 1721, 1925, 3159], 'present': [32, 209], 'study,': [33, 210], 'we': [34, 48, 211, 225, 1930, 3878, 3947, 4141, 4223], 'screened': [35, 212], 'for': [36, 213, 462, 728, 836, 980, 1048, 1074, 1098, 1303, 1515, 1758, 1922, 2022, 2051, 2180, 2756, 3033, 3139, 3338, 3614, 3690, 3750, 4117, 4207], 'multicopy': [37, 214, 1951], 'suppressors': [38, 215], 'that': [39, 110, 147, 216, 287, 324, 619, 828, 924, 1681, 1800, 1882, 1969, 1995, 2008, 2048, 2810, 2998, 3899, 4113, 4195, 4215], 'rescued': [40, 217], 'temperature-sensitive': [41, 218, 1626], 'growth': [42, 82, 125, 219, 259, 302, 1473, 1627, 2781, 3884, 3951, 3965, 3972, 3982, 4106, 4137, 4209], 'VLCFA-limited': [44, 221, 2043], 'yeast': [45, 222, 650, 946, 1594, 1844, 1955, 2010, 2081, 2095, 2689, 2700, 3118, 3135], 'cells,': [46, 223, 1725], 'and': [47, 92, 224, 269, 369, 404, 407, 419, 430, 452, 459, 468, 488, 509, 531, 549, 560, 678, 701, 871, 915, 944, 954, 977, 999, 1045, 1101, 1107, 1157, 1169, 1195, 1204, 1246, 1257, 1281, 1350, 1364, 1458, 1463, 1481, 1609, 1629, 1787, 2085, 2101, 2109, 2178, 2184, 2286, 2328, 2370, 2412, 2454, 2496, 2538, 2580, 2622, 2664, 2713, 2754, 2769, 2817, 2824, 2827, 2836, 2839, 2860, 2882, 2888, 2956, 2978, 2984, 3000, 3008, 3059, 3071, 3094, 3134, 3156, 3235, 3259, 3326, 3334, 3352, 3362, 3368, 3377, 3454, 3514, 3551, 3592, 3625, 3634, 3654, 3678, 3688, 3719, 3741, 3765, 3775, 3799, 3806, 3818, 3829, 3853, 3856, 3910, 3954, 4055, 4135, 4183], 'identified': [49, 226, 1366, 1944], 'VPS21': [51, 228, 1948, 2823], 'gene,': [52, 74, 229, 251, 971, 1039, 2719, 2816, 2948], 'encoding': [53, 230, 1986], 'Rab': [55, 232, 1946], 'GTPase,': [56, 233], 'as': [57, 234, 416, 545, 1277, 1300, 1512, 1949, 2028, 2866, 3065, 3167, 3261, 3396, 3416, 3898, 4155], 'such': [58, 235, 415, 544, 2027, 3897], 'suppressor.': [60, 237], 'When': [61, 238], 'vps21Δ': [63, 103, 240, 280, 1965], 'mutation': [64, 241, 813, 1131, 1675, 3932], 'was': [65, 84, 114, 242, 261, 291, 2126, 2871, 2895, 2967, 2991, 3366, 3466, 3566, 3618, 3640, 3812, 3977, 3990, 4177], 'introduced': [66, 243], 'into': [67, 244, 2698, 2772, 2792, 2853, 2897, 2917, 3017, 3099, 3431], 'deletion': [69, 131, 246, 308, 2166, 2285, 2327, 2369, 2411, 2453, 2495, 2537, 2579, 2621, 2663, 3904], 'mutant': [70, 120, 247, 297, 1315, 1444, 1527, 1956, 1967, 2011, 3887], 'SUR4': [73, 250, 1102, 1677, 1981, 2815, 3095], 'which': [75, 134, 252, 311, 536, 1612, 1795, 1904, 2693, 2935, 4001], 'encodes': [76, 135, 253, 312, 1796], 'VLCFA': [78, 255, 607, 791, 1006, 1161, 1807, 1915, 1941, 1959, 2015, 3875, 3890, 3895, 4010, 4221], 'elongase,': [79, 256], 'synthetic': [81, 124, 258, 301, 381, 2090], 'defect': [83, 126, 260, 303, 1312, 1524, 4107], 'observed.': [85, 262], 'Endosome-mediated': [86, 263], 'vesicular': [87, 264, 1879, 1975, 2030], 'trafficking': [88, 165, 265, 342, 771, 1650, 1705, 1880, 2059], 'pathways,': [89, 166, 266, 343], 'including': [90, 267, 401], 'endocytosis': [91, 268], 'carboxypeptidase': [94, 271, 359], 'Y': [95, 272, 360], '(CPY)': [96, 273], 'pathway,': [97, 274], 'were': [98, 275, 1668, 2075, 2117, 2685, 2696, 2749, 2762, 2770, 2796, 2842, 2864, 3003, 3014, 3063, 3076, 3096, 3114, 3164, 3255, 3304, 3342, 3371, 3402, 3409, 3450, 3532, 3579, 3598, 3656, 3661, 3699, 3731, 3759, 3781, 3840, 4161], 'severely': [99, 276], 'impaired': [100, 277, 1812], 'in': [101, 173, 278, 350, 486, 491, 526, 541, 622, 626, 694, 702, 769, 775, 790, 814, 867, 873, 945, 973, 1016, 1041, 1132, 1173, 1227, 1238, 1261, 1313, 1369, 1420, 1485, 1525, 1566, 1592, 1605, 1632, 1648, 1654, 1676, 1686, 1703, 1709, 1723, 1790, 1811, 1821, 1842, 1862, 1877, 1884, 1895, 1903, 1940, 1973, 1997, 2056, 2066, 2071, 2077, 2089, 2145, 2677, 2692, 2722, 2923, 3040, 3144, 3245, 3312, 3373, 3437, 3583, 3666, 3845, 3876, 3883, 3933, 4004, 4037, 4066, 4151, 4169], 'sur4Δ': [102, 119, 279, 296, 1108, 1724, 1964, 3955, 3975, 4088, 4149, 4172], 'double': [104, 281, 433, 1966, 3903], 'mutants,': [105, 282, 2113], 'while': [106, 283, 1453], 'AP-3': [108, 285], 'pathway': [109, 286, 705, 1596, 1689, 1881], 'bypasses': [111, 288], 'endosome': [113, 290, 2037], 'unaffected.': [115, 292], 'addition,': [117, 294, 1722], 'also': [121, 298, 625, 4102], 'exhibited': [122, 299, 1982, 3963, 4103], 'when': [127, 304], 'combined': [128, 305], 'VPS3,': [133, 310], 'subunit': [137, 314], 'class': [140, 317, 354, 1988], 'C': [141, 318, 355, 1989], 'core': [142, 319, 356, 1990], 'vacuole/endosome': [143, 320, 357, 1991], 'tethering': [144, 321, 358, 1992, 1998], '(CORVET)': [145, 322, 1993], 'complex': [146, 323, 1229, 1236, 1448], 'tethers': [148, 325], 'transport': [149, 326, 1885, 1976, 2033], 'vesicles': [150, 327, 774, 1653, 1708, 2000], 'to': [151, 328, 413, 736, 898, 906, 912, 921, 1224, 1342, 1397, 1733, 1766, 1893, 1936, 2001, 2013, 2879, 2940, 2975, 3116, 3193, 3287, 3307, 3456, 3627, 3647, 3808, 3842, 3870, 4031, 4163, 4166], 'late': [153, 330, 2003], 'endosome/multivesicular': [154, 331, 2004], 'body': [155, 332, 380, 2005], '(MVB).': [156, 333, 2006], 'These': [157, 334, 1666, 3012, 4110, 4192], 'results': [158, 335, 1226, 1810, 4111, 4193], 'suggest': [159, 336, 4194], 'that,': [160, 337], 'all': [162, 339, 1447, 4096], 'intracellular': [164, 341], 'requirement': [167, 344, 2050, 4116, 4138], 'VLCFAs': [169, 346, 454, 523, 692, 831, 840, 851, 1023, 1591, 1682, 1861, 1876, 1970, 2018, 2052, 4118, 4134, 4196], 'is': [170, 347, 410, 608, 620, 726, 940, 947, 1014, 1151, 1340, 1395, 1731, 1756, 1865, 1890, 1920, 2016, 2053, 3191, 3285, 3912, 4002, 4119], 'especially': [171, 348, 2054], 'high': [172, 349, 2055, 3959, 4123], 'endosomal': [175, 352, 2058], 'pathways.': [176, 353, 2060], 'differential': [361], 'interference': [362], 'contrast': [363], 'endoplasmic': [364, 853], 'reticulum': [365, 854], 'glycosylphosphatidylinositol': [366, 627], 'homotypic': [367], 'fusion': [368], 'vacuole': [370, 1735, 2919, 3433], 'protein': [371, 386, 1617, 1819], 'sorting': [372], 'inositol': [373, 1240, 1309, 1521], 'phosphorylceramide': [374, 376, 378, 1310, 1371, 1522], 'mannosylinositol': [375, 1243, 1370], 'mannosyldiinositol': [377, 1247], 'multivesicular': [379], 'dextrose': [382, 2091], 'soluble': [383, 1614], 'NSF': [384, 1615], 'attachment': [385, 1616], 'receptor': [387], 'very': [388, 444, 578, 718, 1076, 1748, 4067], 'acid': [391, 767, 865, 975, 1043, 1079, 1646, 1701, 2108, 3414, 3817, 3852, 4058, 4070], 'Fatty': [392, 864], '(FAs)': [394], 'are': [395, 455, 460, 524, 793, 841, 978, 1046, 1103, 1111, 1232, 1282, 1451, 1683, 1971, 2069, 4205], 'components': [396, 456], 'most': [398, 450], 'cellular': [399, 542, 2025], 'lipids,': [400], 'glycerolipids,': [402], 'sphingolipids,': [403, 1201], 'cholesterol': [405], 'esters,': [406], 'metabolism': [409, 1170, 1258, 1482], 'closely': [411], 'related': [412, 510, 561], 'disorders': [414], 'metabolic': [417], 'syndrome': [418], 'lifestyle-related': [420], 'diseases.': [421], 'FAs': [422, 436, 446, 848, 3303], 'comprise': [423], 'carbon': [424, 614], 'chains': [425], 'highly': [427], 'diverse': [428], 'chain-lengths': [429], 'number': [431], 'bonds,': [434], 'carbons': [442, 931, 4204], 'termed': [443], '(VLCFAs).': [447], 'mammals,': [449], 'saturated': [451, 610], 'monounsaturated': [453], 'sphingolipids': [458, 623, 716, 1172, 1237, 1260, 1449, 1484, 1746], 'important': [461, 2021], 'skin': [463, 492], 'barrier': [464], 'formation,': [465], 'neural': [466], 'functions,': [467], 'functional': [469], 'maintenance': [470, 696], 'liver': [473], '(1Mizutani': [474], 'Y.': [475, 483, 672, 1191, 1193, 1362, 2711, 2912, 3171, 3177, 3181, 3265, 3271, 3275, 3426, 3495, 3505, 4052], 'Mitsutake': [476, 1188, 3178, 3272], 'S.': [477, 759, 961, 1029, 1189, 1356, 1638, 1693, 1831, 2280, 2322, 2364, 2406, 2448, 2490, 2532, 2574, 2616, 2658, 3173, 3179, 3267, 3273, 3478], 'Tsuji': [478], 'K.': [479, 639, 643, 2264, 2306, 2348, 2390, 2432, 2474, 2516, 2558, 2600, 2642, 2906, 2910, 3420, 3424], 'Kihara': [480, 1357, 3184, 3278], 'A.': [481, 502, 553, 645, 859, 863, 1187, 1358, 2154, 2260, 2302, 2344, 2386, 2428, 2470, 2512, 2554, 2596, 2638, 2709, 3185, 3279, 4044, 4050], 'Igarashi': [482, 1192, 1361, 2710, 3180, 3274, 4051], 'Ceramide': [484], 'biosynthesis': [485], 'keratinocyte': [487], 'its': [489, 679], 'role': [490], 'function.Biochimie.': [493], '2009;': [494, 661], '91:': [495], '784-790Crossref': [496], 'PubMed': [497, 516, 567, 598, 664, 686, 752, 782, 822, 880, 993, 1061, 1089, 1140, 1181, 1212, 1269, 1334, 1387, 1438, 1493, 1546, 1584, 1661, 1716, 1782, 1853, 2190, 2292, 2334, 2376, 2418, 2460, 2502, 2544, 2586, 2628, 2670, 2737, 2930, 3047, 3150, 3204, 3298, 3444, 3941, 4082], 'Scopus': [498, 517, 568, 599, 665, 687, 753, 783, 823, 881, 994, 1062, 1090, 1141, 1182, 1213, 1270, 1335, 1388, 1439, 1494, 1547, 1585, 1662, 1717, 1783, 1854, 2191, 2293, 2335, 2377, 2419, 2461, 2503, 2545, 2587, 2629, 2671, 2738, 2931, 3048, 3205, 3299, 3445, 3942, 4083], '(185)': [499], 'Google': [500, 519, 570, 601, 667, 689, 755, 785, 825, 883, 996, 1064, 1092, 1143, 1184, 1215, 1272, 1337, 1390, 1441, 1496, 1549, 1587, 1664, 1719, 1785, 1856, 2193, 2295, 2337, 2379, 2421, 2463, 2505, 2547, 2589, 2631, 2673, 2740, 2933, 3050, 3151, 3207, 3301, 3447, 3944, 4085], 'Scholar–3Kihara': [501], 'acids:': [506, 557], 'elongation,': [507, 558], 'physiology': [508, 559], 'disorders.J.': [511, 562], 'Biochem.': [512, 563], '2012;': [513, 564], '152:': [514, 565], '387-395Crossref': [515, 566], '(241)': [518, 569], 'Scholar).': [520, 602, 690, 786, 884, 997, 1093, 1144, 1216, 1273, 1338, 1391, 1442, 1588, 1665, 1720, 1857, 2741, 3208, 3302, 3448, 4086], 'Mammalian': [521], 'polyunsaturated': [522], 'used': [525, 2045, 2065, 2144], 'generation': [528], 'glycerolipids': [530], 'precursors': [532], 'lipid': [534], 'mediators,': [535], 'have': [537, 2880, 2976], 'many': [538], 'physiological': [539], 'processes,': [543, 2026], 'resolution': [546], 'inflammation': [548], 'retinal': [550], 'functions': [551, 829, 1197, 1883, 3873], '(3Kihara': [552], 'Scholar–5Agbaga': [571], 'M.P.': [572], 'Mandal': [573], 'M.N.': [574], 'Anderson': [575, 2263, 2305, 2347, 2389, 2431, 2473, 2515, 2557, 2599, 2641], 'R.E.': [576], 'Retinal': [577], 'PUFAs:': [580], 'new': [581, 806, 1124, 2679, 3925], 'insights': [582], 'from': [583, 2119, 2169, 2764, 2849, 2875, 2971, 2995, 3476, 3493, 3503, 3901], 'studies': [584], 'on': [585, 1621, 2751, 3382, 3658], 'ELOVL4': [586], 'protein.J.': [587], 'Lipid': [588, 673, 875, 1176, 1207, 1264, 1488], 'Res.': [589, 876, 1177, 1208, 1265, 1489], '2010;': [590, 3201, 3295], '51:': [591], '1624-1642Abstract': [592], 'Full': [593, 595, 747, 749, 988, 990, 1056, 1058, 1084, 1086, 1329, 1331, 1382, 1384, 1433, 1435, 1541, 1543, 1579, 1581, 1777, 1779, 2732, 2734, 4077, 4079], 'Text': [594, 596, 748, 750, 989, 991, 1057, 1059, 1085, 1087, 1330, 1332, 1383, 1385, 1434, 1436, 1542, 1544, 1580, 1582, 1778, 1780, 2733, 2735, 4078, 4080], 'PDF': [597, 751, 992, 1060, 1088, 1333, 1386, 1437, 1545, 1583, 1781, 2736, 4081], '(123)': [600], 'yeast,': [604, 1146, 3877], 'major': [606, 1418, 1564], 'FA': [611, 3161], '26': [617, 4201], '(C26:0)': [618], 'found': [621, 1620], 'but': [624, 723, 1465, 1753, 3589, 4168], '(GPI)': [628], 'anchors': [629], '(6Ejsing': [630], 'C.S.': [631, 957, 1025], 'Sampaio': [632], 'J.L.': [633], 'Surendranath': [634], 'V.': [635, 1067], 'Duchoslav': [636], 'E.': [637, 796, 1114, 2158, 3915], 'Ekroos': [638], 'Klemm': [640], 'R.W.': [641], 'Simons': [642], 'Shevchenko': [644], 'Global': [646], 'analysis': [647], 'lipidome': [651], 'by': [652, 837, 852, 888, 949, 1284, 1320, 1345, 1373, 1400, 1532, 1670, 2128, 2283, 2325, 2367, 2409, 2451, 2493, 2535, 2577, 2619, 2661, 2844, 2873, 2969, 2993, 3078, 3310, 3379, 3404, 3411, 3452, 3642, 3663, 3682, 3728, 3992], 'quantitative': [653], 'shotgun': [654], 'mass': [655], 'spectrometry.Proc.': [656], 'Natl.': [657, 3197, 3291], 'Acad.': [658, 3198, 3292], 'Sci.': [659, 3199, 3293], 'USA.': [660, 3200, 3294], '106:': [662], '2136-2141Crossref': [663], '(714)': [666], 'Scholar,': [668, 1065, 1185, 1497], '7Fujita': [669], 'M.': [670, 798, 802, 1116, 1120, 3030, 3175, 3269, 3917, 3921, 4048], 'Jigami': [671], 'remodeling': [674], 'GPI-anchored': [676, 1818], 'proteins': [677, 3039, 3780], 'function.Biochim.': [680], 'Biophys.': [681, 817, 1135, 3936], 'Acta.': [682, 818, 1136, 3937], '2008;': [683, 2927, 3441, 4074], '1780:': [684], '410-420Crossref': [685], '(99)': [688], 'Yeast': [691, 787, 1094, 1443, 3031], 'function': [693, 972, 1040, 1368, 1674, 1841, 1916, 1996], 'nuclear': [698], 'envelope': [699], 'integrity': [700], 'secretory': [704, 773, 1595, 1622, 1652, 1688, 1707, 1863], '(8Gaigg': [706, 1736], 'B.': [707, 709, 1737, 1739, 2266, 2308, 2350, 2392, 2434, 2476, 2518, 2560, 2602, 2644], 'Timischl': [708, 1738], 'Corbino': [710, 1740], 'L.': [711, 1741, 1826, 1829], 'Schneiter': [712, 1742], 'R.': [713, 861, 1743, 2268, 2270, 2310, 2312, 2352, 2354, 2394, 2396, 2436, 2438, 2478, 2480, 2520, 2522, 2562, 2564, 2604, 2606, 2646, 2648, 3028], 'Synthesis': [714, 1414, 1560, 1744], 'long': [719, 764, 1643, 1698, 1749], 'chain': [720, 765, 1644, 1699, 1750], 'not': [724, 833, 1754, 1909, 4091], 'ergosterol': [725, 1755], 'required': [727, 979, 1047, 1757, 1921, 4206], 'routing': [729, 1759], 'newly': [731, 1365, 1761], 'synthesized': [732, 842, 1283, 1762], 'plasma': [733, 1727, 1763, 1823], 'membrane': [734, 1764, 1824, 3214, 3460, 3465], 'ATPase': [735, 1765], 'cell': [738, 1768, 1845, 3407, 4208], 'surface': [739, 1769], 'yeast.J.': [741, 776, 1655, 1710, 1771], 'Biol.': [742, 778, 983, 1051, 1324, 1377, 1428, 1536, 1574, 1657, 1712, 1772, 1848, 2727, 4072], 'Chem.': [743, 984, 1052, 1325, 1378, 1429, 1537, 1575, 1773, 2728, 4073], '2005;': [744, 1774], '280:': [745, 1775], '22515-22522Abstract': [746, 1776], '(79)': [754, 1784], 'Scholar–11David': [756], 'D.': [757, 1636, 1691, 3026], 'Sundarababu': [758, 1637, 1692], 'Gerst': [760, 1639, 1694], 'J.E.': [761, 1640, 1695], 'Involvement': [762, 1589, 1641, 1696], 'elongation': [768, 845, 886, 976, 1044, 1647, 1702, 1808, 3162, 4011, 4174], 'Cell': [777, 1656, 1711], '1998;': [779, 1658, 1713, 2187], '143:': [780, 1659, 1714], '1167-1182Crossref': [781, 1660, 1715], '(114)': [784, 1663, 1718], 'mutants': [788, 1110, 3962], 'deficient': [789], 'synthesis': [792, 1299, 1372, 1511], 'inviable': [794], '(12Revardel': [795, 1113, 3914], 'Bonneau': [797, 1115, 3916], 'Durrens': [799, 1117, 3918], 'P.': [800, 1118, 2162, 3128, 3919], 'Aigle': [801, 1119, 3920], 'Characterization': [803, 1121, 3922], 'gene': [807, 1125, 1840, 1980, 2182, 2284, 2326, 2368, 2410, 2452, 2494, 2536, 2578, 2620, 2662, 2831, 2870, 2966, 3926], 'family': [808, 1126, 3927], 'developing': [809, 1127, 3928], 'pleiotropic': [810, 1128, 3929], 'phenotypes': [811, 1129, 1667, 3930], 'upon': [812, 1130, 3620, 3931], 'Saccharomyces': [815, 968, 1036, 1133, 1174, 1262, 1318, 1421, 1486, 1530, 1567, 2062, 2170, 2717, 2924, 3145, 3438, 3934], 'cerevisiae.Biochim.': [816, 1134, 3935], '1995;': [819, 1137, 3044, 3938], '1263:': [820, 1138, 3939], '261-265Crossref': [821, 1139, 3940], '(57)': [824, 1142, 3943], 'Scholar),': [826, 1786, 2934, 3051, 3152, 3945], 'indicating': [827], 'cannot': [832], 'be': [834, 1910, 2020], 'substituted': [835], 'FAs.': [839], 'through': [843], 'or': [849, 1021, 1678, 2088, 2132, 3507, 3519, 3541], 'shorter': [850, 1022], '(ER)': [855], 'membrane-embedded': [856], 'enzymes': [857, 1348], '(13Jakobsson': [858], 'Westerberg': [860], 'Jacobsson': [862], 'elongases': [866, 3908], 'mammals:': [868], 'regulation': [870], 'metabolism.Prog.': [874], '2006;': [877, 1178, 1266, 1490, 1850], '45:': [878, 1179, 1267, 1491], '237-249Crossref': [879], '(635)': [882], 'The': [885, 936, 1217, 2061, 2833, 2857, 2868, 2892, 2954, 2988, 3056, 3067, 3089, 3464, 3563, 3638, 3810, 4158], 'proceeds': [887], 'four-step': [890], 'cycle:': [891], 'i)': [892], 'condensation': [893, 938], 'malonyl-CoA': [895], 'acyl-CoA': [897, 923], 'produce': [899], '3-ketoacyl-CoA;': [900], 'ii)': [901], 'reduction': [902, 917, 1631], '3-ketoacyl-CoA': [905], '3-hydroxyacyl-CoA;': [907], 'iii)': [908], 'dehydration': [909], '3-hydroxyacyl-CoA': [911, 1798, 3998, 4063], '2,3-trans': [913, 919], 'enoyl-CoA;': [914], 'iv)': [916], 'enoyl-CoA': [920], 'an': [922, 1158, 1343, 1788, 3469, 3535, 3552], 'has': [925, 1597, 1871], 'two': [929, 950, 1199, 2794], 'more': [930, 1923], 'precursor': [934], 'acyl-CoA.': [935], 'first': [937, 3097, 3879, 3948], 'reaction': [939, 3210, 3249, 3639], 'rate-limiting': [942], 'step': [943, 1804, 4007], 'catalyzed': [948], 'redundant': [951, 1347, 3907], 'elongases,': [952], 'Fen1': [953, 998, 1013], 'Sur4': [955, 1000, 1008], '(14Oh': [956, 1024], 'Toke': [958, 1026], 'D.A.': [959, 1027], 'Mandala': [960, 1028], 'Martin': [962, 1030], 'C.E.': [963, 1031], 'ELO2ELO3,': [964, 1032], 'homologues': [965, 1033], 'cerevisiae': [969, 1037, 1319, 1531, 2063, 2171, 2281, 2323, 2365, 2407, 2449, 2491, 2533, 2575, 2617, 2659, 2718], 'ELO1': [970, 1038], 'sphingolipid': [981, 1049, 1148, 1419, 1565, 3195, 3289], 'formation.J.': [982, 1050], '1997;': [985, 1053, 1326, 1430, 1538, 1576], '272:': [986, 1054, 1327, 1431, 1539, 1577], '17376-17384Abstract': [987, 1055], '(395)': [995, 1063], 'determine': [1001], 'length': [1003], 'products.': [1007], 'produces': [1009], 'C26': [1010], 'VLCFA,': [1011], 'whereas': [1012], 'involved': [1015, 1685, 1939, 1972, 2721, 4003, 4065], 'production': [1018, 3187, 3281], 'C24': [1020, 3189, 3194, 3283, 3288], '15Denic': [1066], 'Weissman': [1068], 'J.S.': [1069], 'A': [1070, 1836, 2742, 3129], 'caliper': [1072], 'mechanism': [1073], 'determining': [1075], 'length.Cell.': [1080], '2007;': [1081, 1209], '130:': [1082], '663-677Abstract': [1083], '(205)': [1091], 'cells': [1095, 1445, 1602, 2684, 3655, 3957, 3976, 3985, 4089, 4101, 4150, 4160, 4173], 'carrying': [1096], 'deletions': [1097, 1604], 'both': [1099, 1461, 1606], 'FEN1': [1100, 3093, 3909], 'inviable,': [1104, 1452], 'yet': [1105], 'fen1Δ': [1106, 3953, 4100], 'single': [1109], 'viable': [1112], 'backbone': [1149], 'ceramide': [1150, 1205, 1225], 'composed': [1152], 'base': [1156, 2097, 2725], 'amide': [1159], 'bond-linked': [1160], '(16Dickson': [1162, 1250, 1474], 'R.C.': [1163, 1251, 1297, 1405, 1475, 1509, 1551], 'Sumanasekera': [1164, 1252, 1476], 'C.': [1165, 1253, 1477], 'Lester': [1166, 1254, 1294, 1412, 1478, 1506, 1558], 'R.L.': [1167, 1255, 1295, 1413, 1479, 1507, 1559], 'Functions': [1168, 1256, 1480], 'cerevisiae.Prog.': [1175, 1263, 1487], '447-465Crossref': [1180, 1268, 1492], '(212)': [1183, 1271, 1495], '17Kihara': [1186], 'Mizutani': [1190, 3176, 3270], 'Metabolism': [1194], 'phosphorylated': [1200], 'sphingosine': [1202], '1-phosphate': [1203], '1-phosphate.Prog.': [1206], '46:': [1210], '126-144Crossref': [1211], '(138)': [1214], 'addition': [1218, 3621, 3643], 'polar': [1221, 1279], 'head': [1222], 'group': [1223, 1280], 'sphingolipid.': [1230], 'There': [1231], 'three': [1233], 'classes': [1234], 'yeast:': [1239], 'phosphorylceramides': [1241, 1244, 1248], '(IPC),': [1242], '(MIPC),': [1245], '[M(IP)2C]': [1249], 'IPCs': [1274, 1467], 'contain': [1275], 'phosphoinositol': [1276, 1394], 'Aur1': [1285], '(18Nagiec': [1286], 'M.M.': [1287, 1411, 1499, 1557], 'Nagiec': [1288, 1406, 1410, 1500, 1552, 1556], 'E.E.': [1289, 1407, 1501, 1553], 'Baltisberger': [1290, 1502], 'J.A.': [1291, 1503], 'Wells': [1292, 1408, 1504, 1554], 'G.B.': [1293, 1409, 1505, 1555], 'Dickson': [1296, 1508], 'Sphingolipid': [1298, 1510], 'target': [1302, 1514], 'antifungal': [1304, 1516], 'drugs.': [1305, 1517], 'Complementation': [1306, 1518], 'synthase': [1311, 1523], 'strain': [1316, 1528], 'AUR1': [1322, 1534], 'gene.J.': [1323, 1427, 1535, 1573], '9809-9817Abstract': [1328, 1540], '(324)': [1336, 1548], 'Mannose': [1339], 'added': [1341], 'IPC': [1344], 'Csg1': [1349], 'Csh1,': [1351], 'generating': [1352], 'MIPC': [1354, 1399], '(19Uemura': [1355], 'Inokuchi': [1359], 'J.': [1360, 2160], 'Csg1p': [1363], 'Csh1p': [1367], 'interacting': [1374], 'Csg2p.J.': [1376], '2003;': [1379], '278:': [1380], '45049-45055Abstract': [1381], '(74)': [1389], 'An': [1392], 'additional': [1393], 'transferred': [1396, 3115, 3455], 'Ipt1,': [1401], 'producing': [1402, 2950, 3153], 'M(IP)2C': [1403], '(20Dickson': [1404], 'mannose-(inositol-P)2-ceramide,': [1416, 1562], 'cerevisiae,': [1422, 1568], 'requires': [1423, 1569], 'IPT1': [1425, 1571], '(YDR072c)': [1426, 1572], '29620-29625Abstract': [1432, 1578], '(146)': [1440, 1586], 'lacking': [1446, 1455, 1460, 3590], '(aur1Δ)': [1450], 'those': [1454, 1459], 'M(IP)2Cs': [1456, 1464], '(ipt1Δ)': [1457], 'MIPCs': [1462], 'containing': [1466, 3586], '(csg1Δ': [1468], 'csh1Δ)': [1469], 'exhibit': [1470, 1624, 4216], 'normal': [1471, 3964, 4035], 'vegetative': [1472], '18Nagiec': [1498], 'Scholar–20Dickson': [1550], 'been': [1598], 'reported.': [1599], 'For': [1600], 'example,': [1601], 'harboring': [1603], 'SNC1': [1608], 'SNC2': [1610], 'genes,': [1611], 'encode': [1613], 'receptors': [1618], '(SNARE)': [1619], 'vesicles,': [1623], 'phenotype': [1628, 3989], 'invertase': [1633], 'secretion': [1634], '(11David': [1635, 1690], 'suppressed': [1669], 'loss': [1672], 'FEN1,': [1679], 'implying': [1680], 'potentially': [1684], 'membrane-localized': [1728], 'H+-ATPase': [1729], 'Pma1': [1730], 'mistargeted': [1732], 'interruption': [1789], 'transcription': [1792], 'PHS1,': [1794], 'dehydratase': [1799, 3999, 4064], 'catalyzes': [1801], 'third': [1803, 4006], 'cycle,': [1809, 4012], 'accumulation': [1813], 'mature': [1815], 'Gas1,': [1816], 'localized': [1820], '(9Yu': [1825], 'Pena': [1827], 'Castillo': [1828], 'Mnaimneh': [1830], 'Hughes': [1832], 'T.R.': [1833], 'Brown': [1834], 'G.W.': [1835], 'survey': [1837], 'essential': [1839, 2024, 4056], 'division': [1846], 'cycle.Mol.': [1847], 'Cell.': [1849], '17:': [1851], '4736-4747Crossref': [1852], '(73)': [1855], 'Thus,': [1858, 1912], 'involvement': [1859, 1874], 'pathways': [1864], 'now': [1866], 'recognized.': [1867], 'no': [1869, 4181], 'report': [1870], 'clearly': [1872], 'demonstrated': [1873], 'toward': [1886], 'vacuole.': [1888], 'This': [1889, 3988], 'probably': [1891], 'due': [1892], 'difficulties': [1894], 'assessing': [1896], 'importance': [1898], 'under': [1899, 1917, 2040, 3858], 'VLCFA-null,': [1900], 'lethal': [1901, 3913], 'conditions,': [1902], 'any': [1905, 2009, 4127], 'indirect': [1906], 'effects': [1907], 'could': [1908, 4090], 'excluded.': [1911], 'investigation': [1913], 'mild': [1918], 'conditions': [1919, 2044], 'understanding.': [1924], 'study': [1927, 2068], 'presented': [1928], 'here,': [1929, 2046], 'carried': [1931], 'out': [1932], 'genetic': [1934, 3042, 4014], 'screen': [1935], 'identify': [1937, 4213], 'genes': [1938, 1985, 2826, 2848, 3075, 4214], 'We': [1943], 'GTPase': [1947], 'suppressor': [1952], 'limited': [1958], 'synthesis.': [1960, 3891], 'Analyses': [1961], 'suggested': [1968], 'Vps21-related': [1974], 'processes.': [1977], 'Furthermore,': [1978], 'interactions': [1983], 'subunits': [1994], 'Considering': [2007], 'unable': [2012], 'synthesize': [2014], 'nonviable,': [2017], 'must': [2019], 'several': [2023], 'general': [2029], 'transport.': [2031], 'defects': [2034], 'appeared': [2038], 'even': [2039, 4036], 'mild,': [2042], 'suggesting': [2047], 'strains': [2064, 2143, 2167, 2177, 3137], 'this': [2067, 2146], 'listed': [2070], 'Table': [2072], '1.': [2073], 'Cells': [2074, 3578, 3594, 3660, 3839], 'grown': [2076, 3580], 'YPD': [2078, 2752], 'medium': [2079, 2093, 3585, 3847], '(1%': [2080], 'extract,': [2082], '2%': [2083, 2086, 2102, 3674, 3720], 'peptone,': [2084], 'D-glucose)': [2087, 2103], '(SD)': [2092], '(0.67%': [2094], 'nitrogen': [2096], 'without': [2098, 2959], 'amino': [2099, 4057], 'supplemented': [2104, 3848], '0.5%': [2106, 3850], 'casamino': [2107, 3851], 'appropriate': [2110, 3587, 3854], 'supplements.': [2111], 'Single-deletion': [2112], 'except': [2114], 'MRY106': [2115], '(vps19Δ::LEU2),': [2116], 'purchased': [2118], 'Open': [2120, 2675], 'Biosystems': [2121], '(Huntsville,': [2122], 'AL).': [2123], 'Gene': [2124], 'disruption': [2125, 2183], 'performed': [2127, 3165], 'replacing': [2129], 'entirety': [2131], 'part': [2133], 'coding': [2136, 2957, 3069], 'region': [2137], 'marker': [2140], 'gene.TABLE': [2141], '1Yeast': [2142], 'studyStrainGenotypeSourceBY4741MATa': [2147], 'his3Δ1': [2148], 'leu2Δ0': [2149], 'met15Δ0': [2150], 'ura3Δ0(46Brachmann': [2151], 'C.B.': [2152], 'Davies': [2153], 'Cost': [2155], 'G.J.': [2156], 'Caputo': [2157], 'Li': [2159], 'Hieter': [2161, 3127], 'Boeke': [2163, 2271, 2313, 2355, 2397, 2439, 2481, 2523, 2565, 2607, 2649], 'J.D.': [2164, 2272, 2314, 2356, 2398, 2440, 2482, 2524, 2566, 2608, 2650], 'Designer': [2165], 'derived': [2168], 'S288C:': [2172], 'useful': [2174], 'set': [2175], 'plasmids': [2179, 2795, 2841, 2863, 3062, 3113], 'PCR-mediated': [2181], 'other': [2185, 2819], 'applications.Yeast.': [2186], '14:': [2188], '115-132Crossref': [2189], '(2613)': [2192], 'Scholar)YOK2230BY4741,': [2194], 'PtetO7-PHS1::NatNT2': [2195, 2199, 2203], 'ura3Δ::MET15This': [2196, 2200, 2204], 'studyMRY7BY4741,': [2197], 'fen1Δ::KanMX4': [2198], 'studyMRY9BY4741,': [2201], 'sur4Δ::KanMX4': [2202], 'studyMRY90BY4741,': [2205], 'vps21Δ::NatNT2': [2206, 2238, 2241, 2244], 'fen1Δ::KanMX4This': [2207], 'studyMRY91BY4741,': [2208], 'vps21': [2209], 'Δ::NatNT2': [2210], 'sur4': [2211], 'Δ::KanMX4This': [2212], 'studyMRY106BY4741,': [2213], 'vps19': [2214], 'Δ::LEU2This': [2215], 'studyMRY121BY4741,': [2216], 'vps3Δ::KanMX4': [2217], 'sur4Δ::NatNT2This': [2218, 2221, 2230, 2233, 2236, 2253], 'studyMRY124BY4741,': [2219], 'vps19Δ::LEU2': [2220, 2252], 'studyMRY129BY4741,': [2222], 'csg1Δ::natNT2': [2223], 'csh1Δ::LEU2This': [2224, 2246], 'studyMRY134BY4741,': [2225], 'ipt1Δ::KanMX4': [2226], 'vps21Δ::NatNT2This': [2227], 'studyMRY135BY4741,': [2228], 'vps8Δ::KanMX4': [2229, 2248, 2251], 'studyMRY136BY4741,': [2231], 'vps39Δ::KanMX4': [2232], 'studyMRY137BY4741,': [2234], 'vps41Δ::KanMX4': [2235], 'studyMRY157BY4741,': [2237], 'csg1Δ::KanMX4This': [2239], 'studyMRY159BY4741,': [2240], 'csh1Δ::KanMX4This': [2242], 'studyMRY160BY4741,': [2243], 'csg1Δ::KanMX4': [2245], 'studyMRY235BY4741,': [2247], 'vps19Δ::LEU2This': [2249], 'studyMRY242BY4741,': [2250], 'study405BY4741,': [2254], 'vps8Δ::KanMX4(47Winzeler': [2255], 'E.A.': [2256, 2298, 2340, 2382, 2424, 2466, 2508, 2550, 2592, 2634], 'Shoemaker': [2257, 2299, 2341, 2383, 2425, 2467, 2509, 2551, 2593, 2635], 'D.D.': [2258, 2300, 2342, 2384, 2426, 2468, 2510, 2552, 2594, 2636], 'Astromoff': [2259, 2301, 2343, 2385, 2427, 2469, 2511, 2553, 2595, 2637], 'Liang': [2261, 2303, 2345, 2387, 2429, 2471, 2513, 2555, 2597, 2639], 'H.': [2262, 2274, 2304, 2316, 2346, 2358, 2388, 2400, 2430, 2442, 2472, 2484, 2514, 2526, 2556, 2568, 2598, 2610, 2640, 2652, 4046], 'Andre': [2265, 2307, 2349, 2391, 2433, 2475, 2517, 2559, 2601, 2643], 'Bangham': [2267, 2309, 2351, 2393, 2435, 2477, 2519, 2561, 2603, 2645], 'Benito': [2269, 2311, 2353, 2395, 2437, 2479, 2521, 2563, 2605, 2647], 'Bussey': [2273, 2315, 2357, 2399, 2441, 2483, 2525, 2567, 2609, 2651], 'et': [2275, 2317, 2359, 2401, 2443, 2485, 2527, 2569, 2611, 2653], 'al.Functional': [2276, 2318, 2360, 2402, 2444, 2486, 2528, 2570, 2612, 2654], 'characterization': [2277, 2319, 2361, 2403, 2445, 2487, 2529, 2571, 2613, 2655, 2714], 'genome': [2282, 2324, 2366, 2408, 2450, 2492, 2534, 2576, 2618, 2660], 'parallel': [2287, 2329, 2371, 2413, 2455, 2497, 2539, 2581, 2623, 2665], 'analysis.Science.': [2288, 2330, 2372, 2414, 2456, 2498, 2540, 2582, 2624, 2666], '1999;': [2289, 2331, 2373, 2415, 2457, 2499, 2541, 2583, 2625, 2667], '285:': [2290, 2332, 2374, 2416, 2458, 2500, 2542, 2584, 2626, 2668], '901-906Crossref': [2291, 2333, 2375, 2417, 2459, 2501, 2543, 2585, 2627, 2669], '(3183)': [2294, 2336, 2378, 2420, 2462, 2504, 2546, 2588, 2630, 2672], 'Scholar)1865BY4741,': [2296], 'vps21Δ::KanMX4(47Winzeler': [2297], 'Scholar)2771BY4741,': [2338], 'csg1Δ::KanMX4(47Winzeler': [2339], 'Scholar)3300BY4741,': [2380], 'csh1Δ::KanMX4(47Winzeler': [2381], 'Scholar)3774BY4741,': [2422], 'vps39Δ::KanMX4(47Winzeler': [2423], 'Scholar)4007BY4741,': [2464], 'ipt1Δ::KanMX4(47Winzeler': [2465], 'Scholar)4015BY4741,': [2506], 'vps41Δ::KanMX4(47Winzeler': [2507], 'Scholar)4329BY4741,': [2548], 'vps3Δ::KanMX4(47Winzeler': [2549], 'Scholar)5281BY4741,': [2590], 'sur4Δ::KanMX4(47Winzeler': [2591], 'Scholar)5763BY4741,': [2632], 'fen1Δ::KanMX4(47Winzeler': [2633], 'Scholar)': [2674], 'table': [2676], 'tab': [2680], 'MRY9': [2681, 2783], '(PtetO7-PHS1': [2682], 'sur4Δ)': [2683], 'transfected': [2686], 'genomic': [2690, 2694, 2877, 2973], 'library,': [2691], 'DNAs': [2695], 'cloned': [2697, 2896, 3015, 3098], 'expression': [2701, 3036, 3119], 'vector': [2702, 3103, 3120], 'pRS426': [2703, 2855], '(2': [2704], 'μ,': [2705], 'URA3': [2706, 3123], 'marker)': [2707, 3124], '(21Kihara': [2708], 'Identification': [2712], 'RSB1,': [2720], 'sphingoid': [2723], 'release.J.': [2726], '2002;': [2729], '277:': [2730], '30048-30054Abstract': [2731], '(81)': [2739], 'pool': [2743], 'approximately': [2745, 4032], '30,000': [2746], 'Ura+': [2747], 'clones': [2748, 2767], 'plated': [2750], 'plates': [2753, 3388], 'incubated': [2755, 3467, 3732], '4': [2757], 'days': [2758], '39°C.': [2760, 2785, 4109, 4211], 'Plasmids': [2761], 'prepared': [2763, 3410], '16': [2766, 2776, 3751], 'obtained': [2768], 'reintroduced': [2771], 'MRY9.': [2773], 'Of': [2774], 'plasmids,': [2777], '2': [2778], 'reproducibly': [2779], 'restored': [2780], 'Sequences': [2786], 'DNA': [2789, 2878, 2974, 3143], 'fragments': [2790, 3013, 3091, 3109], 'inserted': [2791], 'determined': [2797], 'using': [2798, 3534, 3568, 3831, 3885, 4013, 4153], '3130': [2800], 'Genetic': [2801], 'Analyzer': [2802], '(Applied': [2803], 'Biosystems,': [2804], 'Foster': [2805], 'City,': [2806], 'CA).': [2807], 'Sequencing': [2808], 'revealed': [2809], 'one': [2811], 'plasmid': [2812, 2852, 2904, 3024], 'contained': [2813, 2821, 3212], '(pMR22)': [2820], 'YVC1': [2825], 'asparagine': [2829], 'tRNA': [2830], 'tN(GUU)O2.': [2832], 'pMR31': [2834], '(VPS21)': [2835], 'pMR30': [2837], '[YVC1': [2838], 'tN(GUU)O2]': [2840], 'created': [2843], 'subcloning': [2845], 'respective': [2847], 'pMR22': [2851], 'vector.': [2856], 'pOK489': [2858, 2952], '(mRFP-CPS1)': [2859], 'pOK529': [2861, 3054], '(STE2-mRFP)': [2862], 'constructed': [2865, 3064], 'follows.': [2867, 3066], 'CPS1': [2869, 2947], 'amplified': [2872, 2893, 2968, 2992, 3077, 3090], 'PCR': [2874, 2970, 2994, 3079], 'BY4741': [2876, 2972], 'EcoRI': [2881], 'XhoI': [2883, 3001], 'sites': [2884, 2980, 3002], '5′': [2887, 2983, 3007, 3087], '3′': [2889, 2985, 3009], 'ends,': [2890, 2986, 3010], 'respectively.': [2891, 2987, 3011, 3637], 'fragment': [2894, 3523], 'EcoRI-XhoI': [2899], 'site': [2900, 3020, 3084], 'pOK107': [2903, 2996], '(22Obara': [2905, 3419], 'Noda': [2907, 3421], 'T.': [2908, 3183, 3277, 3422], 'Niimi': [2909, 3423], 'Ohsumi': [2911, 3425], 'Transport': [2913, 3427], 'phosphatidylinositol': [2915, 3429], '3-phosphate': [2916, 3430], 'via': [2920, 3434], 'autophagic': [2921, 3435], 'membranes': [2922, 3436], 'cerevisiae.Genes': [2925, 3439], 'Cells.': [2926, 3440], '13:': [2928, 3442], '537-547Crossref': [2929, 3443], '(111)': [2932, 3446], 'contains': [2936], 'mRFP-2xFYVE': [2938], 'domain,': [2939], 'replace': [2941], '2xFYVE': [2943], 'domain': [2944], 'thereby': [2949], 'plasmid.': [2953, 3055], 'promoter': [2955, 4020, 4025], 'region,': [2958, 3070], 'stop': [2961], 'codon,': [2962], 'STE2': [2965], 'SacI': [2977], 'BamHI': [2979, 2999, 3083], 'mRFP': [2989], 'sequence': [2990], 'so': [2997, 3946], 'generated': [3004], 'together': [3016], 'SacI-XhoI': [3019], 'p416TEF': [3023], '(23Mumberg': [3025], 'Muller': [3027], 'Funk': [3029], 'vectors': [3032, 3133], 'controlled': [3035], 'heterologous': [3038], 'different': [3041], 'backgrounds.Gene.': [3043], '156:': [3045], '119-122Crossref': [3046], '(1590)': [3049], 'creating': [3052], 'pMR8': [3057, 3155], '(FEN1)': [3058], 'pMR10': [3060, 3157], '(SUR4)': [3061], 'promoter,': [3068], '3′-UTR': [3072], 'these': [3074], 'creation': [3081], 'end.': [3088], 'pGEM-T': [3101], 'Easy': [3102], '(Promega,': [3104], 'Madison,': [3105], 'WI),': [3106], 'then': [3107, 3305, 3343, 3515], 'BamHI-NotI': [3108], 'resulting': [3112, 3695, 3900], 'pRS316': [3121], '(CEN,': [3122], '(24Sikorski': [3125], 'R.S.': [3126], 'system': [3130], 'shuttle': [3132], 'host': [3136], 'designed': [3138], 'efficient': [3140], 'manipulation': [3141], 'cerevisiae.Genetics.': [3146], '1989;': [3147], '122:': [3148], '19-27Crossref': [3149], 'plasmids.': [3158], 'vitro': [3160, 4152], 'assays': [3163], 'essentially': [3166], 'described': [3168, 3262, 3417], 'previously': [3169, 3263, 3418], '(25Ohno': [3170, 3264], 'Suto': [3172, 3266], 'Yamanaka': [3174, 3268], 'Sassa': [3182, 3276], 'ELOVL1': [3186, 3280], 'acyl-CoAs': [3190, 3284], 'linked': [3192, 3286], 'synthesis.Proc.': [3196, 3290], '107:': [3202, 3296], '18439-18444Crossref': [3203, 3297], '(239)': [3206, 3300], 'Typical': [3209], 'mixtures': [3211], 'total': [3213], 'fractions': [3215], '(20': [3216], 'μg': [3217], 'protein),': [3218], 'μM': [3220, 3233, 3237], 'palmitoyl-CoA': [3221, 4154, 4165], 'complexed': [3222], '0.2': [3224], 'mg/ml': [3225], 'FA-free': [3226], 'BSA': [3227], '(Sigma,': [3228], 'St.': [3229], 'Louis,': [3230], 'MO),': [3231], '73': [3232], 'malonyl-CoA,': [3234], '27.3': [3236], '[14C]malonyl-CoA': [3238], '(0.075': [3239], 'μCi;': [3240], 'Moravek': [3241], 'Biochemicals,': [3242], 'Brea,': [3243], 'CA)': [3244], '50': [3247], 'μl': [3248, 3314, 3323, 3328, 3347, 3350, 3354, 3375, 3743], 'mixture.': [3250], 'After': [3251, 3723, 3757], 'reaction,': [3253], 'lipids': [3254, 3401], 'saponified,': [3256], 'acidified,': [3257], 'extracted,': [3258], 'dried': [3260], 'converted': [3306], 'methyl': [3308], 'esters': [3309], 'dissolving': [3311], '67': [3313], '3': [3316, 3691], 'M': [3317], 'methanolic-HCl': [3318], '(final': [3319, 3331], '1': [3320, 3339, 3635, 3702], 'M),': [3321], '123': [3322], 'chloroform,': [3325, 3351], '10': [3327], '2,2-dimethoxypropane': [3330], 'concentration': [3332, 3630, 3650], '5%),': [3333], 'incubating': [3335], '100°C': [3337], 'h.': [3340], 'Samples': [3341], 'treated': [3344, 3821], '233': [3346], 'methanol,': [3348, 3820], '177': [3349], '300': [3353], '1%': [3356, 3679], '(w/v)': [3357], 'KCl': [3358], 'vigorous': [3360], 'mixing,': [3361], 'organic': [3364], 'phase': [3365, 3844], 'recovered': [3367], 'dried.': [3369], 'Lipids': [3370], 'suspended': [3372, 3665], 'chloroform': [3376], 'separated': [3378, 3451], 'reverse-phase': [3380], 'TLC': [3381, 3387], 'LKC18': [3383], 'Silica': [3384], 'Gel': [3385], '60': [3386], '(Whatman,': [3389], 'Kent,': [3390], 'UK)': [3391], 'chloroform/methanol/water': [3393], '(5:15:1,': [3394], 'v/v)': [3395], 'solvent': [3398], 'system.': [3399], 'Labeled': [3400], 'detected': [3403, 3533, 3830], 'autoradiography.': [3405], 'Total': [3406], 'lysates': [3408, 3696], 'alkaline/trichloroacetic': [3413], 'method': [3415], 'Proteins': [3449], 'SDS-PAGE': [3453], 'Immobilon': [3457], 'polyvinylidene': [3458], 'difluoride': [3459], '(Millipore,': [3461], 'Billerica,': [3462], 'MA).': [3463], 'anti-DsRed': [3470], '(1:1000': [3471], 'dilution;': [3472, 3489, 3499, 3525], 'kind': [3474, 3491, 3501], 'gift': [3475, 3492, 3502], 'Dr.': [3477, 3494, 3504], 'Nishikawa),': [3479], 'anti-CPY': [3480, 3737], '(0.25': [3481, 3509], 'μg/ml;': [3482, 3510], 'Molecular': [3483, 3511], 'Probes,': [3484], 'Eugene,': [3485], 'OR),': [3486], 'anti-Pep4': [3487], '(1:5000': [3488, 3498], 'Ohsumi),': [3496, 3506], 'anti-ALP': [3497], 'anti-Pgk1': [3508], 'Probes)': [3512, 3740], 'antibody,': [3513], 'HRP-conjugated': [3517], 'anti-rabbit': [3518], 'anti-mouse': [3520], 'IgG': [3521], 'F(ab′)2': [3522], '(1:10,000': [3524], 'GE': [3526], 'Healthcare': [3527, 3539, 3748, 3827], 'Bio-Sciences,': [3528], 'Piscataway,': [3529], 'NJ).': [3530], 'Signals': [3531], 'ECL': [3536], 'prime': [3537], '(GE': [3538, 3747, 3826], 'Bio-Sciences)': [3540, 3749], 'Western': [3542], 'Lightning': [3543], 'Plus-ECL': [3544], '(PerkinElmer': [3545], 'Life': [3546, 3610], 'Sciences,': [3547], 'Waltham,': [3548], 'MA)': [3549], 'kit': [3550], 'ImageQuant': [3553], 'LAS': [3554], '4000': [3555], 'Biomolecular': [3556], 'Imager': [3557], '(Fuji': [3558, 3836], 'Photo': [3559, 3837], 'Film,': [3560], 'Tokyo,': [3561], 'Japan).': [3562, 3577], 'Pep4': [3564], 'signal': [3565], 'enhanced': [3567, 3991], 'Can': [3569], 'Get': [3570], 'Signal': [3571], 'Immunoreaction': [3572], 'Enhancer': [3573], 'Solution': [3574], '(TOYOBO,': [3575], 'Osaka,': [3576], '30°C': [3582, 3613], 'SD': [3584, 3846], 'supplements': [3588, 3855], 'methionine': [3591, 3624], 'cysteine.': [3593], '(2.0': [3595], 'A600': [3596], 'units)': [3597], 'pulse-labeled': [3599], '100': [3601], 'μCi': [3602], '[35S]methionine/cysteine': [3603], '(EasyTag': [3604], 'EXPRE35S35S': [3605], 'Protein': [3606, 3745], 'Labeling': [3607], 'Mix;': [3608], 'PerkinElmer': [3609], 'Sciences)': [3611], '15': [3615, 3742], 'min.': [3616, 3692], 'Chase': [3617], 'initiated': [3619], 'unlabeled': [3623], 'cysteine': [3626], 'final': [3629, 3649], '5': [3632], 'mM': [3633, 3670, 3707, 3712, 3715, 3771, 3777, 3789], 'mM,': [3636, 3653], 'terminated': [3641], 'sodium': [3645], 'azide': [3646], 'kept': [3657], 'ice.': [3659], 'collected': [3662], 'centrifugation,': [3664, 3729], 'lysis': [3667], 'buffer': [3668, 3705, 3764, 3769, 3787], '[50': [3669, 3706], 'Tris-HCl': [3671, 3708, 3772], '(pH': [3672, 3709, 3717, 3773, 3791], '6.8),': [3673, 3792], 'SDS,': [3675, 3794], '10%': [3676, 3815], 'glycerol,': [3677, 3796], '2-mercaptoethanol],': [3680], 'broken': [3681], 'vigorously': [3683], 'mixing': [3684], 'glass': [3686], 'beads,': [3687], 'boiled': [3689], 'Then': [3693], '(30': [3697], 'μl)': [3698], 'diluted': [3700], 'ml': [3703], 'Triton': [3704, 3721, 3763], '8.0),': [3710, 3718], '150': [3711, 3776], 'NaCl,': [3713], '0.1': [3714], 'EDTA': [3716], 'X-100].': [3722], 'removal': [3724], 'insoluble': [3726], 'materials': [3727], 'samples': [3730], '750': [3734], 'ng': [3735], 'antibody': [3738], '(Molecular': [3739], 'G-Sepharose': [3746], 'h': [3752], '4°C': [3754], 'rotation.': [3756], 'beads': [3758], 'washed': [3760], 'once': [3761, 3766], 'Wash': [3768], '[10': [3770], '8.0)': [3774], 'NaCl],': [3778], 'bound': [3779], 'eluted': [3782], '2×': [3784], 'SDS': [3785], 'sample': [3786], '[100': [3788], 'Tris-HCl,': [3790], '4%': [3793], '20%': [3795, 3819], '12%': [3797], '2-mercaptoethanol,': [3798], 'trace': [3801], 'amount': [3802], 'bromophenol': [3804], 'blue]': [3805], 'subjected': [3807], 'SDS-PAGE.': [3809], 'gel': [3811], 'fixed': [3813], 'acetic': [3816], 'Amplify': [3823], 'fluorographic': [3824], 'reagent': [3825], 'Bio-Sciences),': [3828], 'bioimaging': [3833], 'analyzer': [3834], 'BAS-2500': [3835], 'Film).': [3838], 'cultured': [3841], 'log': [3843], 'observed': [3857, 4139], 'fluorescence': [3860], 'microscope': [3861], '(Axioimager': [3862], 'M2,': [3863], 'Carl': [3864], 'Zeiss,': [3865], 'Oberkochen,': [3866], 'Germany).': [3867], 'To': [3868, 4125, 4212], 'begin': [3869], 'understand': [3871], 'examined': [3880, 3949, 4142], 'reduced': [3889], 'Complete': [3892], 'abrogation': [3893], 'synthesis,': [3896], 'SUR4,': [3911], 'single-mutant': [3956], 'temperature.': [3960], 'Both': [3961], '30°C;': [3967], 'however,': [3968], '39°C': [3970, 4094], 'retarded': [3978], 'compared': [3979], 'wild-type': [3984], '(Fig.': [3986, 4097, 4190], '1A).': [3987, 4098], 'decreasing': [3993], 'levels': [3995, 4030], 'Phs1,': [4000, 4061], 'manipulation.': [4015], 'Replacement': [4016], 'PHS1': [4019], 'doxycycline-dependent': [4023], 'tetO7': [4024], '(PtetO7)': [4026], 'reduces': [4027], 'Phs1': [4029], '1/20': [4033], 'absence': [4039], 'doxycycline': [4041], 'treatment': [4042], '(26Kihara': [4043], 'Sakuraba': [4045], 'Ikeda': [4047], 'Denpoh': [4049], 'Membrane': [4053], 'topology': [4054], 'residues': [4059], 'elongation.J.': [4071], '283:': [4075], '11199-11209Abstract': [4076], '(50)': [4084], 'PtetO7-PHS1': [4087, 4099, 4148, 4159, 4171], 'grow': [4092], 'severe': [4105], 'indicate': [4112], 'cells’': [4115], 'particularly': [4120], 'significant': [4121], 'temperatures.': [4124], 'reveal': [4126], 'relationship': [4128, 4219], 'between': [4129], 'above,': [4140], 'elongase': [4144], 'activity': [4145], 'substrate.': [4157], 'able': [4162], 'elongate': [4164], 'C26-CoA,': [4167], 'beyond': [4175], 'C22-CoA': [4176], 'impaired,': [4179], 'C26-CoA': [4182], 'only': [4184], 'small': [4185], 'amounts': [4186], 'C24-CoA': [4188], 'produced': [4189], '1B).': [4191], 'and/or': [4202], '24': [4203], 'close': [4218], 'function,': [4222]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2103190727', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}, {'year': 2022, 'cited_by_count': 2}, {'year': 2021, 'cited_by_count': 1}, {'year': 2020, 'cited_by_count': 4}, {'year': 2018, 'cited_by_count': 2}, {'year': 2017, 'cited_by_count': 4}, {'year': 2016, 'cited_by_count': 5}, {'year': 2015, 'cited_by_count': 3}, {'year': 2014, 'cited_by_count': 6}], 'updated_date': '2024-12-08T05:27:03.286910', 'created_date': '2016-06-24'}