Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2102801292', 'doi': 'https://doi.org/10.1128/jcm.42.7.3164-3168.2004', 'title': 'Multiplex Real-Time PCR for Detection of <i>Anaplasma phagocytophilum</i> and <i>Borrelia burgdorferi</i>', 'display_name': 'Multiplex Real-Time PCR for Detection of <i>Anaplasma phagocytophilum</i> and <i>Borrelia burgdorferi</i>', 'publication_year': 2004, 'publication_date': '2004-07-01', 'ids': {'openalex': 'https://openalex.org/W2102801292', 'doi': 'https://doi.org/10.1128/jcm.42.7.3164-3168.2004', 'mag': '2102801292', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/15243077', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/446246'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1128/jcm.42.7.3164-3168.2004', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S53087977', 'display_name': 'Journal of Clinical Microbiology', 'issn_l': '0095-1137', 'issn': ['0095-1137', '1070-633X', '1098-660X'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320263', 'host_organization_name': 'American Society for Microbiology', 'host_organization_lineage': ['https://openalex.org/P4310320263'], 'host_organization_lineage_names': ['American Society for Microbiology'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'green', 'oa_url': 'https://doi.org/10.1128/jcm.42.7.3164-3168.2004', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5082206098', 'display_name': 'Joshua W. Courtney', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I1289490764', 'display_name': 'Centers for Disease Control and Prevention', 'ror': 'https://ror.org/042twtr12', 'country_code': 'US', 'type': 'government', 'lineage': ['https://openalex.org/I1289490764', 'https://openalex.org/I1299022934']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Joshua W. Courtney', 'raw_affiliation_strings': ['Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., MS G-13, Atlanta, GA 30333, USA.'], 'affiliations': [{'raw_affiliation_string': 'Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., MS G-13, Atlanta, GA 30333, USA.', 'institution_ids': ['https://openalex.org/I1289490764']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5057071144', 'display_name': 'Leah M. Kostelnik', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I1289490764', 'display_name': 'Centers for Disease Control and Prevention', 'ror': 'https://ror.org/042twtr12', 'country_code': 'US', 'type': 'government', 'lineage': ['https://openalex.org/I1289490764', 'https://openalex.org/I1299022934']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Leah M. Kostelnik', 'raw_affiliation_strings': ['Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia'], 'affiliations': [{'raw_affiliation_string': 'Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia', 'institution_ids': ['https://openalex.org/I1289490764']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5108347216', 'display_name': 'Nordin S. Zeidner', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I1289490764', 'display_name': 'Centers for Disease Control and Prevention', 'ror': 'https://ror.org/042twtr12', 'country_code': 'US', 'type': 'government', 'lineage': ['https://openalex.org/I1289490764', 'https://openalex.org/I1299022934']}, {'id': 'https://openalex.org/I4210128683', 'display_name': 'Vector (United States)', 'ror': 'https://ror.org/036y4q615', 'country_code': 'US', 'type': 'company', 'lineage': ['https://openalex.org/I4210128683']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Nordin S. Zeidner', 'raw_affiliation_strings': ['Bacterial Zoonoses Branch, Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado'], 'affiliations': [{'raw_affiliation_string': 'Bacterial Zoonoses Branch, Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado', 'institution_ids': ['https://openalex.org/I1289490764', 'https://openalex.org/I4210128683']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5066389785', 'display_name': 'Robert F. Massung', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I1289490764', 'display_name': 'Centers for Disease Control and Prevention', 'ror': 'https://ror.org/042twtr12', 'country_code': 'US', 'type': 'government', 'lineage': ['https://openalex.org/I1289490764', 'https://openalex.org/I1299022934']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Robert F. Massung', 'raw_affiliation_strings': ['Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia'], 'affiliations': [{'raw_affiliation_string': 'Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia', 'institution_ids': ['https://openalex.org/I1289490764']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 3.371, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 439, 'citation_normalized_percentile': {'value': 0.936847, 'is_in_top_1_percent': False, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 99, 'max': 100}, 'biblio': {'volume': '42', 'issue': '7', 'first_page': '3164', 'last_page': '3168'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10296', 'display_name': 'Vector-borne infectious diseases', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/2405', 'display_name': 'Parasitology'}, 'field': {'id': 'https://openalex.org/fields/24', 'display_name': 'Immunology and Microbiology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10296', 'display_name': 'Vector-borne infectious diseases', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/2405', 'display_name': 'Parasitology'}, 'field': {'id': 'https://openalex.org/fields/24', 'display_name': 'Immunology and Microbiology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T12047', 'display_name': 'Viral Infections and Vectors', 'score': 0.9997, 'subfield': {'id': 'https://openalex.org/subfields/2725', 'display_name': 'Infectious Diseases'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T12833', 'display_name': 'Bartonella species infections research', 'score': 0.9987, 'subfield': {'id': 'https://openalex.org/subfields/2405', 'display_name': 'Parasitology'}, 'field': {'id': 'https://openalex.org/fields/24', 'display_name': 'Immunology and Microbiology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/anaplasma-phagocytophilum', 'display_name': 'Anaplasma phagocytophilum', 'score': 0.98054194}, {'id': 'https://openalex.org/keywords/ixodes-scapularis', 'display_name': 'Ixodes scapularis', 'score': 0.6353669}, {'id': 'https://openalex.org/keywords/multiplex', 'display_name': 'Multiplex', 'score': 0.52926004}], 'concepts': [{'id': 'https://openalex.org/C2776370125', 'wikidata': 'https://www.wikidata.org/wiki/Q292189', 'display_name': 'Anaplasma phagocytophilum', 'level': 4, 'score': 0.98054194}, {'id': 'https://openalex.org/C2779159148', 'wikidata': 'https://www.wikidata.org/wiki/Q2723634', 'display_name': 'Borrelia burgdorferi', 'level': 3, 'score': 0.9147564}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.7895489}, {'id': 'https://openalex.org/C2781453105', 'wikidata': 'https://www.wikidata.org/wiki/Q1497962', 'display_name': 'Ixodes scapularis', 'level': 4, 'score': 0.6353669}, {'id': 'https://openalex.org/C159047783', 'wikidata': 'https://www.wikidata.org/wiki/Q7215', 'display_name': 'Virology', 'level': 1, 'score': 0.61642927}, {'id': 'https://openalex.org/C2780508971', 'wikidata': 'https://www.wikidata.org/wiki/Q115528', 'display_name': 'Borrelia', 'level': 4, 'score': 0.6128307}, {'id': 'https://openalex.org/C89423630', 'wikidata': 'https://www.wikidata.org/wiki/Q7193', 'display_name': 'Microbiology', 'level': 1, 'score': 0.58330834}, {'id': 'https://openalex.org/C2779553505', 'wikidata': 'https://www.wikidata.org/wiki/Q845770', 'display_name': 'Spirochaetaceae', 'level': 4, 'score': 0.56682914}, {'id': 'https://openalex.org/C2777479583', 'wikidata': 'https://www.wikidata.org/wiki/Q70288', 'display_name': 'Anaplasma', 'level': 3, 'score': 0.53433466}, {'id': 'https://openalex.org/C2781188995', 'wikidata': 'https://www.wikidata.org/wiki/Q6934982', 'display_name': 'Multiplex', 'level': 2, 'score': 0.52926004}, {'id': 'https://openalex.org/C2777553296', 'wikidata': 'https://www.wikidata.org/wiki/Q201989', 'display_name': 'Lyme disease', 'level': 2, 'score': 0.44594398}, {'id': 'https://openalex.org/C2779620486', 'wikidata': 'https://www.wikidata.org/wiki/Q10304508', 'display_name': 'Tick', 'level': 2, 'score': 0.294609}, {'id': 'https://openalex.org/C159654299', 'wikidata': 'https://www.wikidata.org/wiki/Q79460', 'display_name': 'Antibody', 'level': 2, 'score': 0.09689617}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.09334266}], 'mesh': [{'descriptor_ui': 'D041081', 'descriptor_name': 'Anaplasma phagocytophilum', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': True}, {'descriptor_ui': 'D025065', 'descriptor_name': 'Borrelia burgdorferi', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': True}, {'descriptor_ui': 'D016133', 'descriptor_name': 'Polymerase Chain Reaction', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D041081', 'descriptor_name': 'Anaplasma phagocytophilum', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001425', 'descriptor_name': 'Bacterial Outer Membrane Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001425', 'descriptor_name': 'Bacterial Outer Membrane Proteins', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D025065', 'descriptor_name': 'Borrelia burgdorferi', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D018922', 'descriptor_name': 'HL-60 Cells', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016133', 'descriptor_name': 'Polymerase Chain Reaction', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012336', 'descriptor_name': 'RNA, Ribosomal, 16S', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012336', 'descriptor_name': 'RNA, Ribosomal, 16S', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D012680', 'descriptor_name': 'Sensitivity and Specificity', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 4, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1128/jcm.42.7.3164-3168.2004', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S53087977', 'display_name': 'Journal of Clinical Microbiology', 'issn_l': '0095-1137', 'issn': ['0095-1137', '1070-633X', '1098-660X'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320263', 'host_organization_name': 'American Society for Microbiology', 'host_organization_lineage': ['https://openalex.org/P4310320263'], 'host_organization_lineage_names': ['American Society for Microbiology'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://europepmc.org/articles/pmc446246', 'pdf_url': 'https://europepmc.org/articles/pmc446246?pdf=render', 'source': {'id': 'https://openalex.org/S4306400806', 'display_name': 'Europe PMC (PubMed Central)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1303153112', 'host_organization_name': 'European Bioinformatics Institute', 'host_organization_lineage': ['https://openalex.org/I1303153112'], 'host_organization_lineage_names': ['European Bioinformatics Institute'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC446246', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/15243077', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1128/jcm.42.7.3164-3168.2004', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S53087977', 'display_name': 'Journal of Clinical Microbiology', 'issn_l': '0095-1137', 'issn': ['0095-1137', '1070-633X', '1098-660X'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320263', 'host_organization_name': 'American Society for Microbiology', 'host_organization_lineage': ['https://openalex.org/P4310320263'], 'host_organization_lineage_names': ['American Society for Microbiology'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [{'id': 'https://metadata.un.org/sdg/15', 'score': 0.59, 'display_name': 'Life on land'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 29, 'referenced_works': ['https://openalex.org/W1763519308', 'https://openalex.org/W1865411301', 'https://openalex.org/W1914131521', 'https://openalex.org/W1926303070', 'https://openalex.org/W1950464255', 'https://openalex.org/W1956842074', 'https://openalex.org/W1964077940', 'https://openalex.org/W1974853507', 'https://openalex.org/W2045409905', 'https://openalex.org/W2048789160', 'https://openalex.org/W2060540408', 'https://openalex.org/W2063367477', 'https://openalex.org/W2078648743', 'https://openalex.org/W2080454590', 'https://openalex.org/W2083417553', 'https://openalex.org/W2097262679', 'https://openalex.org/W2101027486', 'https://openalex.org/W2104648036', 'https://openalex.org/W2108928309', 'https://openalex.org/W2113832787', 'https://openalex.org/W2116737756', 'https://openalex.org/W2117908184', 'https://openalex.org/W2133943017', 'https://openalex.org/W2138507128', 'https://openalex.org/W2146707036', 'https://openalex.org/W2156253974', 'https://openalex.org/W2158591166', 'https://openalex.org/W2160976992', 'https://openalex.org/W2166508628'], 'related_works': ['https://openalex.org/W4321609599', 'https://openalex.org/W3196391721', 'https://openalex.org/W2161741245', 'https://openalex.org/W2105371471', 'https://openalex.org/W2034388260', 'https://openalex.org/W2030450153', 'https://openalex.org/W2021425404', 'https://openalex.org/W2007756426', 'https://openalex.org/W1993285206', 'https://openalex.org/W1929008460'], 'abstract_inverted_index': {'ABSTRACT': [0], 'A': [1], 'multiplex': [2, 210], 'real-time': [3, 209], 'PCR': [4, 89, 197, 211], 'assay': [5, 20, 46, 80, 122, 178, 212], 'was': [6, 21, 47, 130, 143, 179], 'developed': [7], 'for': [8, 53, 123, 217, 225], 'the': [9, 45, 57, 79, 103, 121, 175, 204, 218, 233, 240], 'simultaneous': [10, 219], 'detection': [11], 'of': [12, 78, 85, 105, 107, 120, 133, 174, 207, 223, 232], 'Anaplasma': [13, 25], 'phagocytophilum': [14, 55, 93, 110, 126, 139, 227], 'and': [15, 31, 40, 44, 56, 71, 113, 127, 140, 151, 156, 159, 186, 220, 228], 'Borrelia': [16, 27, 58, 229], 'burgdorferi': [17, 63, 98, 129, 142], '.': [18], 'The': [19, 75, 117, 177], 'tested': [22, 60, 180], 'on': [23, 37, 170, 181], 'various': [24], ',': [26, 28, 30, 43, 64, 67, 70, 94, 99], 'Erhlichia': [29], 'Rickettsia': [32], 'species,': [33, 230], 'as': [34, 36], 'well': [35], 'Bartonella': [38], 'henselae': [39], 'Escherichia': [41], 'coli': [42], 'found': [48], 'to': [49, 83, 165, 188, 193], 'be': [50, 215], 'highly': [51], 'specific': [52], 'A.': [54, 92, 109, 125, 138, 226], 'species': [59], '(': [61, 91], 'B.': [62, 65, 68, 72, 97, 128, 141], 'parkeri': [66], 'andersonii': [69], 'bissettii': [73], ').': [74], 'analytical': [76], 'sensitivity': [77, 171], 'is': [81, 203], 'comparable': [82], 'that': [84, 213], 'previously': [86, 194], 'described': [87, 195], 'nested': [88], 'assays': [90], '16S': [95], 'rRNA;': [96], 'fla': [100], 'gene),': [101], 'amplifying': [102], 'equivalent': [104], 'one-eighth': [106], 'an': [108], '-infected': [111], 'cell': [112], '50': [114], 'borrelia': [115], 'spirochetes.': [116], 'dynamic': [118], 'range': [119], 'both': [124], '≥4': [131], 'logs': [132], 'magnitude.': [134], 'Purified': [135], 'DNA': [136, 146], 'from': [137, 148, 152], 'spiked': [144], 'into': [145], 'extracted': [147], 'uninfected': [149], 'ticks': [150, 185], 'negative': [153], 'control': [154], 'mouse': [155], 'human': [157], 'bloods,': [158], 'these': [160], 'background': [161], 'DNAs': [162], 'were': [163], 'shown': [164, 187], 'have': [166, 189], 'no': [167], 'significant': [168], 'effect': [169], 'or': [172], 'specificity': [173], 'assay.': [176], 'field-collected': [182], 'Ixodes': [183], 'scapularis': [184], '100%': [190], 'concordance': [191], 'compared': [192], 'non-probe-based': [196], 'assays.': [198], 'To': [199], 'our': [200], 'knowledge,': [201], 'this': [202], 'first': [205], 'report': [206], 'a': [208], 'can': [214], 'used': [216], 'rapid': [221], 'screening': [222], 'samples': [224], 'two': [231], 'most': [234], 'common': [235], 'tick-borne': [236], 'infectious': [237], 'agents': [238], 'in': [239], 'United': [241], 'States.': [242]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2102801292', 'counts_by_year': [{'year': 2024, 'cited_by_count': 32}, {'year': 2023, 'cited_by_count': 32}, {'year': 2022, 'cited_by_count': 28}, {'year': 2021, 'cited_by_count': 30}, {'year': 2020, 'cited_by_count': 26}, {'year': 2019, 'cited_by_count': 34}, {'year': 2018, 'cited_by_count': 42}, {'year': 2017, 'cited_by_count': 19}, {'year': 2016, 'cited_by_count': 19}, {'year': 2015, 'cited_by_count': 23}, {'year': 2014, 'cited_by_count': 28}, {'year': 2013, 'cited_by_count': 25}, {'year': 2012, 'cited_by_count': 28}], 'updated_date': '2024-12-18T23:43:50.360214', 'created_date': '2016-06-24'}