Title: The effects of BA and NAA on multiplication of Butterwort (Pinguicula gigantea) in vitro
Abstract: The effects of BA and NAA on multiplication of Butterwort (Pinguicula gigantea) in vitro was studied. Leaves were cultured on MS medium, supplemented with 0.2, 2.0, 10.0 and 20.0 mg/l BA and 0.1, 1.0, 5.0 and 10.0 mg/l NAA, 3% sucrose, 0.8% agar. The pH of the medium was adjusted to 5.7. It was found that the maximum number of shoots (21.75 shoots) were obtained from the medium with 2.0 mg/l BA and 0.1 mg/l NAA when cultured on media for 8 weeks. The heaviest weight of explants was 0.57 g. which achieved from the medium with 2.0 mg/l BA and 5.0 mg/l NAA. The MS medium with 0.2 mg/l BA and 5.0 mg/l NAA gave the average of longest shoots (0.83 cm).
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 3
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