Title: High frequency gene conversion among benomyl resistant transformants in the entomopathogenic fungus<i>Metarhizium anisopliae</i>
Abstract: Three different methods, (i) PEG, (ii) electroporation and (iii) biolistic, were employed to transform Metarhizium anisopliae using benomyl resistance as a selectable marker. Transformation frequencies and mitotic stability varied for each method, from 0.8 to 6.9 transformants µg−1 of DNA and 46%, respectively, by the PEG method; 1.3 to 1.8 transformants µg−1 of DNA and 67% by electroporation; and 32 to 201 transformants µg−1 of DNA and 90% by biolistic. We demonstrate by PCR that 60% of the transformants were generated by gene conversion.
Publication Year: 1996
Publication Date: 1996-08-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 26
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