Title: High frequency regeneration and shoot multiplication in Andrographis lineata wall. ex. nees : an endemic medicinal plant of south India
Abstract: An attempt was made to micropropagate the potential medicinal plant Andrographis lineata Wall. ex. Nees using shoot tip and nodal explants. Maximum number of shoots were produced from shoot tip explants (25.7 ± 0.11 shoots /explants) in the optimized concentration of the MS medium containing 1.5 mg/l BAP in combination with 30.0 mg/l AdS compared to the nodal explants (15.0 ± 0.19 shoots/explants). The juvenile in vitro regenerated shoots were elongated (7.0 ± 0.6 cm) in 0.3 mg/l GA 3. Rooting was best induced in MS medium supplemented with 1.0 mg/l IBA. The plantlets were successfully hardened in the plant growth chamber and later transferred to greenhouse at 70% survival rate.
Publication Year: 2011
Publication Date: 2011-09-30
Language: en
Type: article
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