Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2095920248', 'doi': 'https://doi.org/10.1111/j.1462-2920.2004.00702.x', 'title': 'Detection and quantification of the human‐specific HF183 <i>Bacteroides</i> 16S rRNA genetic marker with real‐time PCR for assessment of human faecal pollution in freshwater', 'display_name': 'Detection and quantification of the human‐specific HF183 <i>Bacteroides</i> 16S rRNA genetic marker with real‐time PCR for assessment of human faecal pollution in freshwater', 'publication_year': 2004, 'publication_date': '2004-11-19', 'ids': {'openalex': 'https://openalex.org/W2095920248', 'doi': 'https://doi.org/10.1111/j.1462-2920.2004.00702.x', 'mag': '2095920248', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/15658992'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1111/j.1462-2920.2004.00702.x', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S6919857', 'display_name': 'Environmental Microbiology', 'issn_l': '1462-2912', 'issn': ['1462-2912', '1462-2920'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'green', 'oa_url': 'https://biblio.ugent.be/publication/300306/file/870168', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5081849090', 'display_name': 'Sylvie Seurinck', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I32597200', 'display_name': 'Ghent University', 'ror': 'https://ror.org/00cv9y106', 'country_code': 'BE', 'type': 'education', 'lineage': ['https://openalex.org/I32597200']}], 'countries': ['BE'], 'is_corresponding': False, 'raw_author_name': 'Sylvie Seurinck', 'raw_affiliation_strings': ['Laboratory of Microbial Ecology and Technology, LabMET, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium'], 'affiliations': [{'raw_affiliation_string': 'Laboratory of Microbial Ecology and Technology, LabMET, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium', 'institution_ids': ['https://openalex.org/I32597200']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5061586713', 'display_name': 'Tom Defoirdt', 'orcid': 'https://orcid.org/0000-0002-7446-2246'}, 'institutions': [{'id': 'https://openalex.org/I32597200', 'display_name': 'Ghent University', 'ror': 'https://ror.org/00cv9y106', 'country_code': 'BE', 'type': 'education', 'lineage': ['https://openalex.org/I32597200']}], 'countries': ['BE'], 'is_corresponding': False, 'raw_author_name': 'Tom Defoirdt', 'raw_affiliation_strings': ['Laboratory of Microbial Ecology and Technology, LabMET, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium'], 'affiliations': [{'raw_affiliation_string': 'Laboratory of Microbial Ecology and Technology, LabMET, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium', 'institution_ids': ['https://openalex.org/I32597200']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5090222404', 'display_name': 'Willy Verstraete', 'orcid': 'https://orcid.org/0000-0002-3140-3713'}, 'institutions': [{'id': 'https://openalex.org/I32597200', 'display_name': 'Ghent University', 'ror': 'https://ror.org/00cv9y106', 'country_code': 'BE', 'type': 'education', 'lineage': ['https://openalex.org/I32597200']}], 'countries': ['BE'], 'is_corresponding': False, 'raw_author_name': 'Willy Verstraete', 'raw_affiliation_strings': ['Laboratory of Microbial Ecology and Technology, LabMET, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium'], 'affiliations': [{'raw_affiliation_string': 'Laboratory of Microbial Ecology and Technology, LabMET, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium', 'institution_ids': ['https://openalex.org/I32597200']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5042950202', 'display_name': 'Steven D. Siciliano', 'orcid': 'https://orcid.org/0000-0002-8994-3341'}, 'institutions': [{'id': 'https://openalex.org/I32625721', 'display_name': 'University of Saskatchewan', 'ror': 'https://ror.org/010x8gc63', 'country_code': 'CA', 'type': 'education', 'lineage': ['https://openalex.org/I32625721']}], 'countries': ['CA'], 'is_corresponding': False, 'raw_author_name': 'Steven D. Siciliano', 'raw_affiliation_strings': ['Department of Soil Science 51 Campus Drive University of Saskatchewan Saskatoon Saskatchewan S7N 5A8 Canada'], 'affiliations': [{'raw_affiliation_string': 'Department of Soil Science 51 Campus Drive University of Saskatchewan Saskatoon Saskatchewan S7N 5A8 Canada', 'institution_ids': ['https://openalex.org/I32625721']}]}], 'institution_assertions': [], 'countries_distinct_count': 2, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 4330, 'currency': 'USD', 'value_usd': 4330, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 20.617, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 347, 'citation_normalized_percentile': {'value': 0.967423, 'is_in_top_1_percent': False, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 98, 'max': 99}, 'biblio': {'volume': '7', 'issue': '2', 'first_page': '249', 'last_page': '259'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11331', 'display_name': 'Fecal contamination and water quality', 'score': 0.9983, 'subfield': {'id': 'https://openalex.org/subfields/2312', 'display_name': 'Water Science and Technology'}, 'field': {'id': 'https://openalex.org/fields/23', 'display_name': 'Environmental Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11331', 'display_name': 'Fecal contamination and water quality', 'score': 0.9983, 'subfield': {'id': 'https://openalex.org/subfields/2312', 'display_name': 'Water Science and Technology'}, 'field': {'id': 'https://openalex.org/fields/23', 'display_name': 'Environmental Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T12259', 'display_name': 'Environmental Justice and Health Disparities', 'score': 0.968, 'subfield': {'id': 'https://openalex.org/subfields/3312', 'display_name': 'Sociology and Political Science'}, 'field': {'id': 'https://openalex.org/fields/33', 'display_name': 'Social Sciences'}, 'domain': {'id': 'https://openalex.org/domains/2', 'display_name': 'Social Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/amplicon', 'display_name': 'Amplicon', 'score': 0.7377928}, {'id': 'https://openalex.org/keywords/primer', 'display_name': 'Primer (cosmetics)', 'score': 0.66405046}, {'id': 'https://openalex.org/keywords/melting-curve-analysis', 'display_name': 'Melting curve analysis', 'score': 0.6129764}, {'id': 'https://openalex.org/keywords/enumeration', 'display_name': 'Enumeration', 'score': 0.52116036}, {'id': 'https://openalex.org/keywords/sybr-green-i', 'display_name': 'SYBR Green I', 'score': 0.43653268}], 'concepts': [{'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.8481256}, {'id': 'https://openalex.org/C8185291', 'wikidata': 'https://www.wikidata.org/wiki/Q2844457', 'display_name': 'Amplicon', 'level': 4, 'score': 0.7377928}, {'id': 'https://openalex.org/C2778168646', 'wikidata': 'https://www.wikidata.org/wiki/Q133186', 'display_name': 'Bacteroides', 'level': 3, 'score': 0.73063195}, {'id': 'https://openalex.org/C42062724', 'wikidata': 'https://www.wikidata.org/wiki/Q1209205', 'display_name': '16S ribosomal RNA', 'level': 3, 'score': 0.7011318}, {'id': 'https://openalex.org/C2777563447', 'wikidata': 'https://www.wikidata.org/wiki/Q7243397', 'display_name': 'Primer (cosmetics)', 'level': 2, 'score': 0.66405046}, {'id': 'https://openalex.org/C48023723', 'wikidata': 'https://www.wikidata.org/wiki/Q856198', 'display_name': 'Real-time polymerase chain reaction', 'level': 3, 'score': 0.61655056}, {'id': 'https://openalex.org/C84133368', 'wikidata': 'https://www.wikidata.org/wiki/Q6813768', 'display_name': 'Melting curve analysis', 'level': 4, 'score': 0.6129764}, {'id': 'https://openalex.org/C49105822', 'wikidata': 'https://www.wikidata.org/wiki/Q176996', 'display_name': 'Polymerase chain reaction', 'level': 3, 'score': 0.5545656}, {'id': 'https://openalex.org/C156340839', 'wikidata': 'https://www.wikidata.org/wiki/Q2704791', 'display_name': 'Enumeration', 'level': 2, 'score': 0.52116036}, {'id': 'https://openalex.org/C67905577', 'wikidata': 'https://www.wikidata.org/wiki/Q215980', 'display_name': 'Ribosomal RNA', 'level': 3, 'score': 0.44583228}, {'id': 'https://openalex.org/C2780968160', 'wikidata': 'https://www.wikidata.org/wiki/Q423692', 'display_name': 'SYBR Green I', 'level': 4, 'score': 0.43653268}, {'id': 'https://openalex.org/C89423630', 'wikidata': 'https://www.wikidata.org/wiki/Q7193', 'display_name': 'Microbiology', 'level': 1, 'score': 0.3956597}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.39379132}, {'id': 'https://openalex.org/C523546767', 'wikidata': 'https://www.wikidata.org/wiki/Q10876', 'display_name': 'Bacteria', 'level': 2, 'score': 0.36646596}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.19899997}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.11136976}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C33923547', 'wikidata': 'https://www.wikidata.org/wiki/Q395', 'display_name': 'Mathematics', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C178790620', 'wikidata': 'https://www.wikidata.org/wiki/Q11351', 'display_name': 'Organic chemistry', 'level': 1, 'score': 0.0}, {'id': 'https://openalex.org/C114614502', 'wikidata': 'https://www.wikidata.org/wiki/Q76592', 'display_name': 'Combinatorics', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D001439', 'descriptor_name': 'Bacteroides', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D001439', 'descriptor_name': 'Bacteroides', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': True}, {'descriptor_ui': 'D004275', 'descriptor_name': 'DNA, Ribosomal', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': True}, {'descriptor_ui': 'D005618', 'descriptor_name': 'Fresh Water', 'qualifier_ui': 'Q000382', 'qualifier_name': 'microbiology', 'is_major_topic': True}, {'descriptor_ui': 'D016133', 'descriptor_name': 'Polymerase Chain Reaction', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D012336', 'descriptor_name': 'RNA, Ribosomal, 16S', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D001439', 'descriptor_name': 'Bacteroides', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D052160', 'descriptor_name': 'Benzothiazoles', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015169', 'descriptor_name': 'Colony Count, Microbial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004269', 'descriptor_name': 'DNA, Bacterial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004269', 'descriptor_name': 'DNA, Bacterial', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': False}, {'descriptor_ui': 'D004275', 'descriptor_name': 'DNA, Ribosomal', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003959', 'descriptor_name': 'Diamines', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005243', 'descriptor_name': 'Feces', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005243', 'descriptor_name': 'Feces', 'qualifier_ui': 'Q000382', 'qualifier_name': 'microbiology', 'is_major_topic': False}, {'descriptor_ui': 'D005618', 'descriptor_name': 'Fresh Water', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D020459', 'descriptor_name': 'Genes, rRNA', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005819', 'descriptor_name': 'Genetic Markers', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008969', 'descriptor_name': 'Molecular Sequence Data', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009930', 'descriptor_name': 'Organic Chemicals', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009930', 'descriptor_name': 'Organic Chemicals', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D011804', 'descriptor_name': 'Quinolines', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012329', 'descriptor_name': 'RNA, Bacterial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012329', 'descriptor_name': 'RNA, Bacterial', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D012336', 'descriptor_name': 'RNA, Ribosomal, 16S', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015203', 'descriptor_name': 'Reproducibility of Results', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012680', 'descriptor_name': 'Sensitivity and Specificity', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017422', 'descriptor_name': 'Sequence Analysis, DNA', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013696', 'descriptor_name': 'Temperature', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013997', 'descriptor_name': 'Time Factors', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014871', 'descriptor_name': 'Water Microbiology', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 3, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1111/j.1462-2920.2004.00702.x', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S6919857', 'display_name': 'Environmental Microbiology', 'issn_l': '1462-2912', 'issn': ['1462-2912', '1462-2920'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': True, 'landing_page_url': 'https://biblio.ugent.be/publication/300306/file/870168', 'pdf_url': 'https://biblio.ugent.be/publication/300306/file/870168', 'source': {'id': 'https://openalex.org/S4306400478', 'display_name': 'Ghent University Academic Bibliography (Ghent University)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I32597200', 'host_organization_name': 'Ghent University', 'host_organization_lineage': ['https://openalex.org/I32597200'], 'host_organization_lineage_names': ['Ghent University'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/15658992', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://biblio.ugent.be/publication/300306/file/870168', 'pdf_url': 'https://biblio.ugent.be/publication/300306/file/870168', 'source': {'id': 'https://openalex.org/S4306400478', 'display_name': 'Ghent University Academic Bibliography (Ghent University)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I32597200', 'host_organization_name': 'Ghent University', 'host_organization_lineage': ['https://openalex.org/I32597200'], 'host_organization_lineage_names': ['Ghent University'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [{'id': 'https://metadata.un.org/sdg/6', 'score': 0.74, 'display_name': 'Clean water and sanitation'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 54, 'referenced_works': ['https://openalex.org/W1490733816', 'https://openalex.org/W1601712888', 'https://openalex.org/W185411816', 'https://openalex.org/W1964641994', 'https://openalex.org/W1973200293', 'https://openalex.org/W1986183109', 'https://openalex.org/W1988317796', 'https://openalex.org/W1989407198', 'https://openalex.org/W1989476640', 'https://openalex.org/W1991750327', 'https://openalex.org/W1995356937', 'https://openalex.org/W2003759137', 'https://openalex.org/W2010838809', 'https://openalex.org/W2011757080', 'https://openalex.org/W2013032478', 'https://openalex.org/W2032851273', 'https://openalex.org/W2037220611', 'https://openalex.org/W2042977092', 'https://openalex.org/W2051489849', 'https://openalex.org/W2054753713', 'https://openalex.org/W2056373708', 'https://openalex.org/W2074095468', 'https://openalex.org/W2075116635', 'https://openalex.org/W2088234365', 'https://openalex.org/W2090608857', 'https://openalex.org/W2100171490', 'https://openalex.org/W2101422212', 'https://openalex.org/W2106441995', 'https://openalex.org/W2106580462', 'https://openalex.org/W2106896822', 'https://openalex.org/W2110425157', 'https://openalex.org/W2115771420', 'https://openalex.org/W2119348211', 'https://openalex.org/W2119578504', 'https://openalex.org/W2121409138', 'https://openalex.org/W2132181413', 'https://openalex.org/W2138950086', 'https://openalex.org/W2142374516', 'https://openalex.org/W2146224715', 'https://openalex.org/W2149007738', 'https://openalex.org/W2150716871', 'https://openalex.org/W2151091336', 'https://openalex.org/W2154168928', 'https://openalex.org/W2161860494', 'https://openalex.org/W2164578725', 'https://openalex.org/W2165483373', 'https://openalex.org/W2171676024', 'https://openalex.org/W2172174856', 'https://openalex.org/W2283438716', 'https://openalex.org/W4206262540', 'https://openalex.org/W4242770708', 'https://openalex.org/W4255077650', 'https://openalex.org/W49147553', 'https://openalex.org/W645275453'], 'related_works': ['https://openalex.org/W2386582890', 'https://openalex.org/W2382697620', 'https://openalex.org/W2355082371', 'https://openalex.org/W2098282209', 'https://openalex.org/W1999336249', 'https://openalex.org/W1995295345', 'https://openalex.org/W1989331162', 'https://openalex.org/W1983367485', 'https://openalex.org/W1973016513', 'https://openalex.org/W1968946391'], 'abstract_inverted_index': {'Summary': [0], 'The': [1, 86, 172, 223], 'human‐specific': [2, 98, 155, 216, 228], 'HF183': [3, 99], 'Bacteriodes': [4], '16S': [5], 'rRNA': [6], 'genetic': [7], 'marker': [8, 32, 53, 156, 230, 252], 'can': [9], 'be': [10, 118], 'used': [11, 166], 'to': [12, 26, 50, 64, 167, 181, 235, 244, 255], 'detect': [13], 'human': [14, 81], 'faecal': [15, 55, 84], 'pollution': [16], 'in': [17, 33, 54, 94, 176, 231], 'water': [18], 'environments.': [19], 'However,': [20], 'there': [21], 'is': [22], 'currently': [23], 'no': [24], 'method': [25], 'quantify': [27, 51], 'the': [28, 61, 89, 97, 104, 107, 149, 169, 184, 197, 205, 227, 251, 256, 259], 'prevalence': [29], 'of': [30, 88, 106, 135, 137, 183, 192, 196, 207, 221, 226, 258], 'this': [31, 52, 154], 'environmental': [34, 57], 'samples.': [35, 58, 85], 'We': [36], 'developed': [37, 91], 'a': [38, 65, 72, 112, 133, 161, 201], 'real‐time': [39, 69, 108, 163], 'polymerase': [40], 'chain': [41], 'reaction': [42], '(PCR)': [43], 'assay': [44, 110, 122, 143, 164], 'using': [45], 'SYBR': [46], 'Green': [47], 'I': [48], 'detection': [49, 170, 174, 190], 'and': [56, 78, 82, 129, 144, 240, 242], 'To': [59], 'decrease': [60, 103], 'amplicon': [62], 'length': [63], 'suitable': [66], 'size': [67], 'for': [68, 233, 262], 'PCR': [70, 109, 128], 'detection,': [71], 'new': [73, 121], 'reverse': [74, 92], 'primer': [75, 93, 100], 'was': [76, 123, 165, 209], 'designed': [77], 'validated': [79], 'on': [80], 'animal': [83], 'use': [87], 'newly': [90], 'combination': [95], 'with': [96, 132, 187], 'did': [101], 'not': [102], 'specificity': [105], 'but': [111], 'melting': [113], 'curve': [114], 'analysis': [115], 'must': [116], 'always': [117], 'included.': [119], 'This': [120], 'more': [124], 'sensitive': [125], 'than': [126, 139], 'conventional': [127], 'highly': [130], 'reproducible': [131], 'coefficient': [134], 'variation': [136], 'less': [138], '1%': [140], 'within': [141], 'an': [142, 188], '3%': [145], 'between': [146], 'assays.': [147], 'As': [148], 'Bacteroides': [150, 217, 229], 'species': [151], 'that': [152, 250], 'carries': [153], 'has': [157], 'never': [158], 'been': [159], 'isolated,': [160], 'bacteria': [162], 'determine': [168], 'efficiency.': [171], 'estimated': [173], 'efficiency': [175, 191], 'freshwater': [177, 232], 'ranged': [178], 'from': [179], '78%': [180], '91%': [182], 'true': [185, 198], 'value': [186], 'average': [189], '83': [193], '±': [194, 211], '4%': [195], 'value.': [199], 'Using': [200], 'simple': [202], 'filtration': [203], 'method,': [204], 'limit': [206], 'quantification': [208], '4.7': [210], '0.3': [212], '×': [213], '10': [214], '5': [215], 'markers': [218], 'per': [219], 'litre': [220], 'freshwater.': [222], 'aerobic': [224], 'incubation': [225, 260, 264], 'up': [234, 243, 254], '24': [236], 'days': [237, 246], 'at': [238, 247], '4': [239], '12°C,': [241], '8': [245], '28°C,': [248], 'indicated': [249], 'persisted': [253], 'end': [257], 'period': [261], 'all': [263], 'temperatures.': [265]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2095920248', 'counts_by_year': [{'year': 2024, 'cited_by_count': 6}, {'year': 2023, 'cited_by_count': 12}, {'year': 2022, 'cited_by_count': 10}, {'year': 2021, 'cited_by_count': 18}, {'year': 2020, 'cited_by_count': 14}, {'year': 2019, 'cited_by_count': 19}, {'year': 2018, 'cited_by_count': 12}, {'year': 2017, 'cited_by_count': 19}, {'year': 2016, 'cited_by_count': 15}, {'year': 2015, 'cited_by_count': 19}, {'year': 2014, 'cited_by_count': 32}, {'year': 2013, 'cited_by_count': 26}, {'year': 2012, 'cited_by_count': 25}], 'updated_date': '2025-01-09T15:43:02.808575', 'created_date': '2016-06-24'}