Publication Information

{'id': 'https://openalex.org/W2093917991', 'doi': 'https://doi.org/10.1006/prep.2000.1373', 'title': 'Rapid Evaluation and Optimization of Recombinant Protein Production Using GFP Tagging', 'display_name': 'Rapid Evaluation and Optimization of Recombinant Protein Production Using GFP Tagging', 'publication_year': 2001, 'publication_date': '2001-02-01', 'ids': {'openalex': 'https://openalex.org/W2093917991', 'doi': 'https://doi.org/10.1006/prep.2000.1373', 'mag': '2093917991', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/11162409'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1006/prep.2000.1373', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S9184782', 'display_name': 'Protein Expression and Purification', 'issn_l': '1046-5928', 'issn': ['1046-5928', '1096-0279'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5068497662', 'display_name': 'Elke Rücker', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I181369854', 'display_name': 'Friedrich-Alexander-Universität Erlangen-Nürnberg', 'ror': 'https://ror.org/00f7hpc57', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I181369854']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Elke Rücker', 'raw_affiliation_strings': ['Institute for Medical and Clinical Virology, University of Erlangen-Nürnberg, Schlossgarten 4, D-91054, Erlangen, Germany'], 'affiliations': [{'raw_affiliation_string': 'Institute for Medical and Clinical Virology, University of Erlangen-Nürnberg, Schlossgarten 4, D-91054, Erlangen, Germany', 'institution_ids': ['https://openalex.org/I181369854']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5058923583', 'display_name': 'Grit Schneider', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I181369854', 'display_name': 'Friedrich-Alexander-Universität Erlangen-Nürnberg', 'ror': 'https://ror.org/00f7hpc57', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I181369854']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Grit Schneider', 'raw_affiliation_strings': ['Institute for Medical and Clinical Virology, University of Erlangen-Nürnberg, Schlossgarten 4, D-91054, Erlangen, Germany'], 'affiliations': [{'raw_affiliation_string': 'Institute for Medical and Clinical Virology, University of Erlangen-Nürnberg, Schlossgarten 4, D-91054, Erlangen, Germany', 'institution_ids': ['https://openalex.org/I181369854']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5111870595', 'display_name': 'Kim Steinhäuser', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I884002606', 'display_name': 'Paul Ehrlich Institut', 'ror': 'https://ror.org/00yssnc44', 'country_code': 'DE', 'type': 'facility', 'lineage': ['https://openalex.org/I884002606']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Kim Steinhäuser', 'raw_affiliation_strings': ['Paul-Ehrich-Institut, Paul-Ehrlich-Str. 51-59, D-63225, Langen, Germany'], 'affiliations': [{'raw_affiliation_string': 'Paul-Ehrich-Institut, Paul-Ehrlich-Str. 51-59, D-63225, Langen, Germany', 'institution_ids': ['https://openalex.org/I884002606']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5039021631', 'display_name': 'Roswitha Löwer', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I884002606', 'display_name': 'Paul Ehrlich Institut', 'ror': 'https://ror.org/00yssnc44', 'country_code': 'DE', 'type': 'facility', 'lineage': ['https://openalex.org/I884002606']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Roswitha Löwer', 'raw_affiliation_strings': ['Paul-Ehrich-Institut, Paul-Ehrlich-Str. 51-59, D-63225, Langen, Germany'], 'affiliations': [{'raw_affiliation_string': 'Paul-Ehrich-Institut, Paul-Ehrlich-Str. 51-59, D-63225, Langen, Germany', 'institution_ids': ['https://openalex.org/I884002606']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5086546362', 'display_name': 'Joachim Hauber', 'orcid': 'https://orcid.org/0000-0001-6219-9527'}, 'institutions': [{'id': 'https://openalex.org/I181369854', 'display_name': 'Friedrich-Alexander-Universität Erlangen-Nürnberg', 'ror': 'https://ror.org/00f7hpc57', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I181369854']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Joachim Hauber', 'raw_affiliation_strings': ['Institute for Medical and Clinical Virology, University of Erlangen-Nürnberg, Schlossgarten 4, D-91054, Erlangen, Germany'], 'affiliations': [{'raw_affiliation_string': 'Institute for Medical and Clinical Virology, University of Erlangen-Nürnberg, Schlossgarten 4, D-91054, Erlangen, Germany', 'institution_ids': ['https://openalex.org/I181369854']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5069285060', 'display_name': 'Roland H. Stauber', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I181369854', 'display_name': 'Friedrich-Alexander-Universität Erlangen-Nürnberg', 'ror': 'https://ror.org/00f7hpc57', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I181369854']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Roland H. Stauber', 'raw_affiliation_strings': ['Institute for Medical and Clinical Virology, University of Erlangen-Nürnberg, Schlossgarten 4, D-91054, Erlangen, Germany'], 'affiliations': [{'raw_affiliation_string': 'Institute for Medical and Clinical Virology, University of Erlangen-Nürnberg, Schlossgarten 4, D-91054, Erlangen, Germany', 'institution_ids': ['https://openalex.org/I181369854']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 3310, 'currency': 'USD', 'value_usd': 3310, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 0.803, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 35, 'citation_normalized_percentile': {'value': 0.809268, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 89, 'max': 90}, 'biblio': {'volume': '21', 'issue': '1', 'first_page': '220', 'last_page': '223'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11663', 'display_name': 'Viral Infectious Diseases and Gene Expression in Insects', 'score': 0.9992, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11663', 'display_name': 'Viral Infectious Diseases and Gene Expression in Insects', 'score': 0.9992, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10540', 'display_name': 'Advanced Fluorescence Microscopy Techniques', 'score': 0.9987, 'subfield': {'id': 'https://openalex.org/subfields/1304', 'display_name': 'Biophysics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11016', 'display_name': 'Monoclonal and Polyclonal Antibodies Research', 'score': 0.9978, 'subfield': {'id': 'https://openalex.org/subfields/2741', 'display_name': 'Radiology, Nuclear Medicine and Imaging'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/target-protein', 'display_name': 'Target protein', 'score': 0.54101694}, {'id': 'https://openalex.org/keywords/fluorescent-protein', 'display_name': 'Fluorescent protein', 'score': 0.4332137}, {'id': 'https://openalex.org/keywords/flag-tag', 'display_name': 'FLAG-tag', 'score': 0.42285046}], 'concepts': [{'id': 'https://openalex.org/C142613039', 'wikidata': 'https://www.wikidata.org/wiki/Q272983', 'display_name': 'Green fluorescent protein', 'level': 3, 'score': 0.9513577}, {'id': 'https://openalex.org/C40767141', 'wikidata': 'https://www.wikidata.org/wiki/Q285697', 'display_name': 'Recombinant DNA', 'level': 3, 'score': 0.7396443}, {'id': 'https://openalex.org/C6856738', 'wikidata': 'https://www.wikidata.org/wiki/Q331672', 'display_name': 'Protein expression', 'level': 3, 'score': 0.63561726}, {'id': 'https://openalex.org/C2780430158', 'wikidata': 'https://www.wikidata.org/wiki/Q7685902', 'display_name': 'Target protein', 'level': 3, 'score': 0.54101694}, {'id': 'https://openalex.org/C169274487', 'wikidata': 'https://www.wikidata.org/wiki/Q840211', 'display_name': 'Fluorescence microscope', 'level': 3, 'score': 0.5408535}, {'id': 'https://openalex.org/C91881484', 'wikidata': 'https://www.wikidata.org/wiki/Q191807', 'display_name': 'Fluorescence', 'level': 2, 'score': 0.47319934}, {'id': 'https://openalex.org/C3019336641', 'wikidata': 'https://www.wikidata.org/wiki/Q55643886', 'display_name': 'Fluorescent protein', 'level': 4, 'score': 0.4332137}, {'id': 'https://openalex.org/C30417494', 'wikidata': 'https://www.wikidata.org/wiki/Q3382226', 'display_name': 'FLAG-tag', 'level': 5, 'score': 0.42285046}, {'id': 'https://openalex.org/C3675279', 'wikidata': 'https://www.wikidata.org/wiki/Q211935', 'display_name': 'Protein biosynthesis', 'level': 2, 'score': 0.42053267}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.4157791}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.4112545}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.37075722}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.36708504}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.3327812}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.14103204}, {'id': 'https://openalex.org/C123894998', 'wikidata': 'https://www.wikidata.org/wiki/Q425158', 'display_name': 'Fusion protein', 'level': 4, 'score': 0.098412395}, {'id': 'https://openalex.org/C121332964', 'wikidata': 'https://www.wikidata.org/wiki/Q413', 'display_name': 'Physics', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C62520636', 'wikidata': 'https://www.wikidata.org/wiki/Q944', 'display_name': 'Quantum mechanics', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D005982', 'descriptor_name': 'Glutathione Transferase', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D008164', 'descriptor_name': 'Luminescent Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': True}, {'descriptor_ui': 'D003001', 'descriptor_name': 'Cloning, Molecular', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': 'Q000166', 'qualifier_name': 'cytology', 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': 'Q000201', 'qualifier_name': 'enzymology', 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': 'Q000254', 'qualifier_name': 'growth & development', 'is_major_topic': False}, {'descriptor_ui': 'D017930', 'descriptor_name': 'Genes, Reporter', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005982', 'descriptor_name': 'Glutathione Transferase', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': False}, {'descriptor_ui': 'D005982', 'descriptor_name': 'Glutathione Transferase', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D005982', 'descriptor_name': 'Glutathione Transferase', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D049452', 'descriptor_name': 'Green Fluorescent Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008164', 'descriptor_name': 'Luminescent Proteins', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': False}, {'descriptor_ui': 'D008164', 'descriptor_name': 'Luminescent Proteins', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': False}, {'descriptor_ui': 'D008856', 'descriptor_name': 'Microscopy, Fluorescence', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': False}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011503', 'descriptor_name': 'Transglutaminases', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': False}, {'descriptor_ui': 'D011503', 'descriptor_name': 'Transglutaminases', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D011503', 'descriptor_name': 'Transglutaminases', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1006/prep.2000.1373', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S9184782', 'display_name': 'Protein Expression and Purification', 'issn_l': '1046-5928', 'issn': ['1046-5928', '1096-0279'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/11162409', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [{'display_name': 'Responsible consumption and production', 'score': 0.4, 'id': 'https://metadata.un.org/sdg/12'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 7, 'referenced_works': ['https://openalex.org/W1519297291', 'https://openalex.org/W2046430129', 'https://openalex.org/W2078680583', 'https://openalex.org/W2095651361', 'https://openalex.org/W2127068865', 'https://openalex.org/W2139065950', 'https://openalex.org/W2144754627'], 'related_works': ['https://openalex.org/W4235287918', 'https://openalex.org/W4224276315', 'https://openalex.org/W38841742', 'https://openalex.org/W2747960173', 'https://openalex.org/W2520631443', 'https://openalex.org/W2380483271', 'https://openalex.org/W2354355953', 'https://openalex.org/W2188507493', 'https://openalex.org/W2130171154', 'https://openalex.org/W1992420603'], 'abstract_inverted_index': None, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2093917991', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}, {'year': 2022, 'cited_by_count': 3}, {'year': 2020, 'cited_by_count': 2}, {'year': 2019, 'cited_by_count': 1}, {'year': 2018, 'cited_by_count': 1}, {'year': 2016, 'cited_by_count': 1}, {'year': 2014, 'cited_by_count': 1}, {'year': 2013, 'cited_by_count': 1}, {'year': 2012, 'cited_by_count': 1}], 'updated_date': '2024-12-10T21:15:32.040597', 'created_date': '2016-06-24'}