The Particulate Methane Monooxygenase from Methylococcus capsulatus (Bath) Is a Novel Copper-containing Three-subunit Enzyme

Name: Work Video:
Duration: 3 min 30 s
Hiep-Hoa T. Nguyen; Sean J. Elliott; John H. K. Yip; Sunney I. Chan
{'id': 'https://openalex.org/W2093768571', 'doi': 'https://doi.org/10.1074/jbc.273.14.7957', 'title': 'The Particulate Methane Monooxygenase from Methylococcus capsulatus (Bath) Is a Novel Copper-containing Three-subunit Enzyme', 'display_name': 'The Particulate Methane Monooxygenase from Methylococcus capsulatus (Bath) Is a Novel Copper-containing Three-subunit Enzyme', 'publication_year': 1998, 'publication_date': '1998-04-01', 'ids': {'openalex': 'https://openalex.org/W2093768571', 'doi': 'https://doi.org/10.1074/jbc.273.14.7957', 'mag': '2093768571', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/9525893'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.273.14.7957', 'pdf_url': 'http://www.jbc.org/article/S002192581852514X/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'hybrid', 'oa_url': 'http://www.jbc.org/article/S002192581852514X/pdf', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5019709220', 'display_name': 'Hiep-Hoa T. Nguyen', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I122411786', 'display_name': 'California Institute of Technology', 'ror': 'https://ror.org/05dxps055', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I122411786']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Hiep-Hoa T. Nguyen', 'raw_affiliation_strings': ['Arthur Amos Noyes Laboratory of Chemical Physics†, California Institute of Technology. Pasadena, California 91125, USA'], 'affiliations': [{'raw_affiliation_string': 'Arthur Amos Noyes Laboratory of Chemical Physics†, California Institute of Technology. Pasadena, California 91125, USA', 'institution_ids': ['https://openalex.org/I122411786']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5013993666', 'display_name': 'Sean J. Elliott', 'orcid': 'https://orcid.org/0000-0003-0096-9551'}, 'institutions': [{'id': 'https://openalex.org/I122411786', 'display_name': 'California Institute of Technology', 'ror': 'https://ror.org/05dxps055', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I122411786']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Sean J. Elliott', 'raw_affiliation_strings': ['From the Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, California 91125'], 'affiliations': [{'raw_affiliation_string': 'From the Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, California 91125', 'institution_ids': ['https://openalex.org/I122411786']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5031136766', 'display_name': 'John H. K. Yip', 'orcid': 'https://orcid.org/0000-0002-0955-4873'}, 'institutions': [{'id': 'https://openalex.org/I122411786', 'display_name': 'California Institute of Technology', 'ror': 'https://ror.org/05dxps055', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I122411786']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'John Hon-Kay Yip', 'raw_affiliation_strings': ['From the Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, California 91125'], 'affiliations': [{'raw_affiliation_string': 'From the Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, California 91125', 'institution_ids': ['https://openalex.org/I122411786']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5017230178', 'display_name': 'Sunney I. Chan', 'orcid': 'https://orcid.org/0000-0002-5348-2723'}, 'institutions': [{'id': 'https://openalex.org/I122411786', 'display_name': 'California Institute of Technology', 'ror': 'https://ror.org/05dxps055', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I122411786']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Sunney I. Chan', 'raw_affiliation_strings': ['From the Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, California 91125'], 'affiliations': [{'raw_affiliation_string': 'From the Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, California 91125', 'institution_ids': ['https://openalex.org/I122411786']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'apc_paid': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'fwci': 4.258, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 211, 'citation_normalized_percentile': {'value': 0.99997, 'is_in_top_1_percent': True, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 98, 'max': 99}, 'biblio': {'volume': '273', 'issue': '14', 'first_page': '7957', 'last_page': '7966'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T12688', 'display_name': 'Microbial metabolism and enzyme function', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T12688', 'display_name': 'Microbial metabolism and enzyme function', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11747', 'display_name': 'Metal-Catalyzed Oxygenation Mechanisms', 'score': 0.9996, 'subfield': {'id': 'https://openalex.org/subfields/1604', 'display_name': 'Inorganic Chemistry'}, 'field': {'id': 'https://openalex.org/fields/16', 'display_name': 'Chemistry'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T11626', 'display_name': 'Metalloenzymes and iron-sulfur proteins', 'score': 0.9851, 'subfield': {'id': 'https://openalex.org/subfields/2105', 'display_name': 'Renewable Energy, Sustainability and the Environment'}, 'field': {'id': 'https://openalex.org/fields/21', 'display_name': 'Energy'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/methane-monooxygenase', 'display_name': 'Methane Monooxygenase', 'score': 0.9459673}, {'id': 'https://openalex.org/keywords/molecular-mass', 'display_name': 'Molecular mass', 'score': 0.6256744}, {'id': 'https://openalex.org/keywords/methanotroph', 'display_name': 'Methanotroph', 'score': 0.50715876}], 'concepts': [{'id': 'https://openalex.org/C23296010', 'wikidata': 'https://www.wikidata.org/wiki/Q417187', 'display_name': 'Methane monooxygenase', 'level': 3, 'score': 0.9459673}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.81399536}, {'id': 'https://openalex.org/C58121356', 'wikidata': 'https://www.wikidata.org/wiki/Q182854', 'display_name': 'Molecular mass', 'level': 3, 'score': 0.6256744}, {'id': 'https://openalex.org/C104292427', 'wikidata': 'https://www.wikidata.org/wiki/Q899781', 'display_name': 'Protein subunit', 'level': 3, 'score': 0.6086765}, {'id': 'https://openalex.org/C2777671786', 'wikidata': 'https://www.wikidata.org/wiki/Q2633954', 'display_name': 'Methanotroph', 'level': 4, 'score': 0.50715876}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.4868714}, {'id': 'https://openalex.org/C41625074', 'wikidata': 'https://www.wikidata.org/wiki/Q176088', 'display_name': 'Membrane', 'level': 2, 'score': 0.48478478}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.47878516}, {'id': 'https://openalex.org/C104577883', 'wikidata': 'https://www.wikidata.org/wiki/Q4065016', 'display_name': 'Anaerobic oxidation of methane', 'level': 3, 'score': 0.19609123}, {'id': 'https://openalex.org/C161790260', 'wikidata': 'https://www.wikidata.org/wiki/Q82264', 'display_name': 'Catalysis', 'level': 2, 'score': 0.12508103}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D008772', 'descriptor_name': 'Methylococcaceae', 'qualifier_ui': 'Q000201', 'qualifier_name': 'enzymology', 'is_major_topic': True}, {'descriptor_ui': 'D010105', 'descriptor_name': 'Oxygenases', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D000595', 'descriptor_name': 'Amino Acid Sequence', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003300', 'descriptor_name': 'Copper', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008772', 'descriptor_name': 'Methylococcaceae', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008969', 'descriptor_name': 'Molecular Sequence Data', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D010105', 'descriptor_name': 'Oxygenases', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D010105', 'descriptor_name': 'Oxygenases', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': False}, {'descriptor_ui': 'D010105', 'descriptor_name': 'Oxygenases', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}], 'locations_count': 3, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.273.14.7957', 'pdf_url': 'http://www.jbc.org/article/S002192581852514X/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.502.5847', 'pdf_url': 'http://authors.library.caltech.edu/10948/1/NGUjbc98.pdf', 'source': {'id': 'https://openalex.org/S4306400349', 'display_name': 'CiteSeer X (The Pennsylvania State University)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I130769515', 'host_organization_name': 'Pennsylvania State University', 'host_organization_lineage': ['https://openalex.org/I130769515'], 'host_organization_lineage_names': ['Pennsylvania State University'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'submittedVersion', 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/9525893', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.273.14.7957', 'pdf_url': 'http://www.jbc.org/article/S002192581852514X/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [{'id': 'https://metadata.un.org/sdg/6', 'score': 0.5, 'display_name': 'Clean water and sanitation'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 57, 'referenced_works': ['https://openalex.org/W1482589774', 'https://openalex.org/W1518243624', 'https://openalex.org/W1551698626', 'https://openalex.org/W1590302505', 'https://openalex.org/W1602201841', 'https://openalex.org/W1847764357', 'https://openalex.org/W1971733876', 'https://openalex.org/W1975085160', 'https://openalex.org/W1977262176', 'https://openalex.org/W1979051901', 'https://openalex.org/W1993430877', 'https://openalex.org/W1994252628', 'https://openalex.org/W1996442918', 'https://openalex.org/W2004962085', 'https://openalex.org/W2007522859', 'https://openalex.org/W2010160430', 'https://openalex.org/W2011225358', 'https://openalex.org/W2011607516', 'https://openalex.org/W2018200555', 'https://openalex.org/W2022397131', 'https://openalex.org/W2029515584', 'https://openalex.org/W2039340029', 'https://openalex.org/W2039823965', 'https://openalex.org/W2041943896', 'https://openalex.org/W2042865863', 'https://openalex.org/W2044428152', 'https://openalex.org/W2050631703', 'https://openalex.org/W2050900897', 'https://openalex.org/W2052736807', 'https://openalex.org/W2056566930', 'https://openalex.org/W2060078841', 'https://openalex.org/W2068431139', 'https://openalex.org/W2073569045', 'https://openalex.org/W2077151104', 'https://openalex.org/W2080391896', 'https://openalex.org/W2082840768', 'https://openalex.org/W2083900920', 'https://openalex.org/W2084624701', 'https://openalex.org/W2087585058', 'https://openalex.org/W2091086834', 'https://openalex.org/W2093061296', 'https://openalex.org/W2100837269', 'https://openalex.org/W2111539104', 'https://openalex.org/W2125277600', 'https://openalex.org/W2132267217', 'https://openalex.org/W2151044016', 'https://openalex.org/W2158087713', 'https://openalex.org/W2168582519', 'https://openalex.org/W2171652074', 'https://openalex.org/W2172844043', 'https://openalex.org/W2203199023', 'https://openalex.org/W2203827897', 'https://openalex.org/W3023485817', 'https://openalex.org/W4210836699', 'https://openalex.org/W4255995127', 'https://openalex.org/W602426647', 'https://openalex.org/W611187948'], 'related_works': ['https://openalex.org/W3087623316', 'https://openalex.org/W2971728017', 'https://openalex.org/W2413452415', 'https://openalex.org/W2396610117', 'https://openalex.org/W2082202122', 'https://openalex.org/W2010160430', 'https://openalex.org/W1993109308', 'https://openalex.org/W1976069560', 'https://openalex.org/W1956910974', 'https://openalex.org/W1493719049'], 'abstract_inverted_index': {'The': [0, 89, 107, 173, 189, 199, 223, 318, 407, 425, 491, 507, 517, 541, 636, 663, 784, 817, 1170, 1224, 1438, 1747, 2294, 2479, 2662, 2780, 3040, 3654, 3676, 3719, 3733, 3759, 3774, 3950, 4176, 4192, 4204, 4211, 4266, 4288, 4426], 'particulate': [1, 319, 738, 1237], 'methane': [2, 27, 320, 345, 638, 648, 698, 723, 731, 735, 739, 1238, 2776, 3631, 3712, 3760, 3767, 3793, 3799, 3804, 3905], 'monooxygenase': [3, 321, 724, 1239], '(pMMO)': [4, 322, 1240], 'is': [5, 201, 225, 252, 265, 323, 519, 543, 570, 583, 788, 1406, 1618, 1725, 1752, 1872, 2010, 2024, 2668, 2803, 2814, 3012, 3045, 3072, 3077, 3324, 3565, 3581, 3768, 4274], 'known': [6, 324, 755, 2749, 2784, 3014, 3046, 3079], 'to': [7, 11, 15, 93, 115, 151, 227, 261, 289, 294, 306, 325, 329, 333, 411, 433, 469, 545, 579, 607, 612, 624, 649, 696, 756, 1242, 1413, 1430, 1741, 1868, 1903, 2197, 2200, 2299, 2654, 2785, 2789, 3015, 3019, 3047, 3080, 3143, 3151, 3202, 3252, 3257, 3300, 3319, 3336, 3357, 3390, 3513, 3527, 3553, 3672, 3684, 3689, 3723, 3738, 3829, 3873, 3882, 3889, 3901, 3911, 3914, 3920, 4018, 4035, 4038, 4054, 4093, 4148, 4187, 4282, 4405, 4418, 4422], 'be': [8, 49, 59, 94, 152, 244, 326, 367, 377, 412, 470, 562, 1243, 1431, 1742, 2198, 2300, 2655, 2786, 3016, 3350, 3455, 3494, 3788, 3822, 3953, 4033, 4249, 4416, 4431, 4452], 'very': [9, 327, 2787, 3017], 'difficult': [10, 328, 2788], 'study': [12, 330, 697], 'mainly': [13, 331, 1411], 'due': [14, 332, 1412], 'its': [16, 196, 334, 514, 1414, 4056], 'unusual': [17, 335, 1415, 2805, 3031], 'activity': [18, 47, 336, 365, 1416, 1424, 2809, 2829, 2836, 3011, 3050, 3071, 3196, 3235, 3392, 3492, 3523, 3928, 4112], 'instability': [19, 337, 2806, 3032], 'in': [20, 34, 41, 68, 98, 195, 338, 352, 359, 386, 416, 513, 641, 1425, 1433, 1479, 1866, 1890, 2003, 2492, 2748, 2769, 2799, 2832, 3036, 3066, 3105, 3109, 3115, 3141, 3207, 3263, 3346, 3462, 3496, 3542, 3599, 3632, 3713, 3728, 3811, 3858, 3962, 3993, 4110, 4142, 4296, 4321, 4357, 4401], 'vitro.': [21, 339], 'By': [22, 340], 'cultivatingMethylococcus': [23, 341], 'capsulatus': [24, 342, 1180, 1896, 3218, 3364, 3596, 3943], '(Bath)': [25, 343, 1181, 1897, 3219, 3365, 3597, 3944], 'under': [26, 344, 1620, 3051, 3626], 'stress': [28, 346, 1622, 3805], 'conditions': [29, 76, 347, 394, 3053], 'and': [30, 55, 74, 85, 122, 132, 159, 169, 175, 206, 285, 313, 348, 373, 392, 403, 440, 450, 477, 487, 493, 524, 603, 631, 659, 700, 766, 799, 1377, 1484, 1494, 1885, 2139, 2267, 2310, 2424, 2489, 2589, 2751, 2778, 2822, 2955, 3096, 3148, 3168, 3200, 3226, 3230, 3344, 3359, 3368, 3400, 3403, 3409, 3420, 3430, 3440, 3448, 3464, 3481, 3511, 3531, 3576, 3618, 3634, 3687, 3695, 3716, 3755, 3869, 3891, 3897, 3980, 4016, 4060, 4097, 4118, 4139, 4162, 4217, 4220, 4260, 4319, 4442], 'high': [31, 349, 3841, 3860, 3937, 4379], 'copper': [32, 350, 1245, 1621, 2205, 2296, 3043, 3058, 3064, 3323, 3345, 3852, 3887, 4048, 4137], 'levels': [33, 351, 3842, 3938], 'the': [35, 42, 95, 111, 140, 147, 183, 231, 241, 274, 307, 353, 360, 413, 429, 458, 465, 501, 549, 559, 592, 625, 645, 1175, 1236, 1419, 1422, 1427, 1474, 1481, 1612, 1616, 1723, 1732, 1735, 1750, 1755, 1765, 1891, 1901, 1904, 2008, 2022, 2201, 2497, 2500, 2645, 2649, 2656, 2757, 2762, 2770, 2796, 2801, 2804, 2808, 2811, 3025, 3038, 3061, 3067, 3082, 3085, 3100, 3111, 3119, 3122, 3193, 3204, 3211, 3253, 3259, 3302, 3305, 3309, 3315, 3325, 3331, 3338, 3340, 3347, 3361, 3372, 3414, 3417, 3425, 3428, 3431, 3452, 3497, 3554, 3557, 3563, 3570, 3574, 3587, 3600, 3608, 3673, 3700, 3746, 3797, 3831, 3834, 3844, 3884, 3904, 3916, 3927, 3933, 3940, 4040, 4045, 4051, 4067, 4095, 4123, 4131, 4150, 4153, 4157, 4189, 4201, 4221, 4229, 4233, 4238, 4241, 4253, 4278, 4327, 4330, 4343, 4352, 4406, 4419], 'growth': [36, 354, 3808], 'medium,': [37, 355], 'membranes': [38, 356, 2482, 3429, 3934, 4206, 4247, 4273, 4280, 4290, 4354], 'highly': [39, 357, 2670, 3030, 3093, 3460, 4143], 'enriched': [40, 358, 3461], 'pMMO': [43, 57, 90, 112, 184, 200, 224, 278, 361, 375, 408, 430, 502, 518, 542, 596, 742, 1428, 1724, 1736, 1745, 1751, 1767, 1905, 2009, 2023, 2763, 2781, 2802, 3070, 3112, 3128, 3215, 3310, 3348, 3362, 3378, 3441, 3463, 3564, 3845, 3861, 4155, 4286], 'with': [44, 124, 161, 362, 442, 479, 1878, 2306, 2684, 3479, 3519, 3525, 3613, 3620, 3705, 3750, 3983, 4264], 'exceptionally': [45, 363, 3466, 3840, 3859], 'stable': [46, 364, 3467, 3520, 3931], 'can': [48, 58, 243, 366, 376, 561, 2764, 3138, 3493, 3787, 4031, 4248, 4414, 4430], 'isolated': [50, 368, 1178, 3433, 3471], 'from': [51, 62, 178, 369, 380, 496, 1899, 3130, 3216, 3434, 3538, 3681], 'these': [52, 63, 99, 179, 301, 370, 381, 417, 497, 619, 3540, 3729, 4269], 'cells.': [53, 371], 'Purified': [54, 372], 'active': [56, 374, 1226, 2759, 3306, 3333, 3447], 'subsequently': [60, 378, 3470, 3688], 'obtained': [61, 379, 3789, 4195], 'membrane': [64, 80, 105, 382, 398, 423, 1892, 3401, 3458, 4098, 4190, 4242, 4427], 'preparations': [65, 383, 3450], 'using': [66, 384, 651, 3220, 3472, 3483, 4312], 'protocols': [67, 385, 3376], 'which': [69, 251, 387, 569, 3837, 4146], 'an': [70, 125, 162, 388, 443, 480, 692, 1758, 3384, 3501, 3627], 'excess': [71, 389], 'of': [72, 103, 110, 117, 129, 139, 146, 154, 166, 182, 233, 240, 276, 390, 421, 428, 435, 447, 457, 464, 472, 484, 500, 551, 558, 594, 647, 665, 704, 722, 794, 1174, 1374, 1421, 1440, 1609, 1734, 1749, 1757, 1761, 1882, 2136, 2203, 2264, 2421, 2487, 2499, 2586, 2648, 2674, 2687, 2761, 2772, 2795, 2807, 2810, 2952, 3027, 3042, 3063, 3069, 3099, 3121, 3127, 3153, 3162, 3210, 3214, 3266, 3308, 3342, 3374, 3387, 3397, 3406, 3416, 3427, 3437, 3451, 3486, 3504, 3508, 3556, 3573, 3586, 3629, 3699, 3745, 3782, 3785, 3818, 3833, 3843, 3939, 4004, 4006, 4013, 4021, 4024, 4047, 4066, 4079, 4106, 4152, 4240, 4255, 4272, 4308, 4324, 4329, 4348, 4372, 4374, 4395, 4397], 'reductants': [73, 391], 'anaerobic': [75, 393], 'were': [77, 221, 395, 539, 1888, 3443, 3469, 3602, 3624, 3669, 3678, 3721, 3736, 3960, 3991, 4081, 4165, 4226, 4244, 4291, 4355], 'maintained': [78, 396, 3495, 3603, 3625, 3954], 'during': [79, 397, 3957, 4156], 'solubilization': [81, 399], 'by': [82, 87, 400, 405, 823, 1754, 2665, 3591, 3971, 4167, 4293, 4317], 'dodecyl': [83, 401], 'β-d-maltoside': [84, 402], 'purification': [86, 404], 'chromatography.': [88, 406], 'was': [91, 113, 149, 273, 409, 431, 467, 591, 2195, 2830, 3149, 3197, 3224, 3236, 3763, 3777, 3827, 3880, 3908, 3929, 3945, 4072, 4178, 4198, 4336, 4345], 'found': [92, 114, 150, 410, 432, 468, 640, 1740, 2196, 3150, 3828], 'major': [96, 414], 'constituent': [97, 415], 'membranes,': [100, 418, 1763, 3836, 4236, 4262], 'constituting': [101, 419, 4237], '60–80%': [102, 420], 'total': [104, 422, 3885], 'proteins.': [106, 424, 4350], 'dominant': [108, 426], 'species': [109, 138, 427, 456, 721], 'consist': [116, 434, 3152], 'three': [118, 155, 236, 436, 473, 554, 3154, 4083], 'subunits,': [119, 156, 437, 474], 'α,': [120, 438], 'β,': [121, 158, 439, 476], 'γ,': [123, 160, 441, 478], 'apparent': [126, 163, 444, 481, 1879], 'molecular': [127, 164, 445, 482, 1880], 'mass': [128, 165, 446, 483], '45,': [130, 448, 1883], '26,': [131, 168, 449, 486], '23': [133, 170, 451, 488], 'kDa,': [134, 171, 452, 489], 'respectively.': [135, 172, 453, 490, 3894], 'A': [136, 246, 454, 564, 3268, 3779], 'second': [137, 455], 'pMMO,': [141, 459, 737, 1615, 3558], 'a': [142, 202, 208, 255, 460, 520, 526, 573, 654, 702, 762, 767, 789, 795, 797, 800, 1229, 1244, 2011, 2025, 2485, 2680, 2766, 3090, 3092, 3156, 3164, 3169, 3221, 3272, 3506, 3566, 3710, 3740, 3791, 4019, 4086, 4313, 4338, 4402], 'proteolytically': [143, 461], 'processed': [144, 462], 'version': [145, 463], 'enzyme,': [148, 466, 3575], 'composed': [153, 471], 'α′,': [157, 475], '35,': [167, 485, 1884], 'α': [174, 492], 'α′': [176, 494], 'subunits': [177, 495], 'two': [180, 498, 4270], 'forms': [181, 239, 282, 303, 499, 557, 600, 621, 3499], 'contain': [185, 503, 2484, 3312, 3839], 'identical': [186, 504], 'N-terminal': [187, 197, 505, 515], 'sequences.': [188, 506], 'γ': [190, 508], 'subunit,': [191, 509, 3167], 'however,': [192, 510], 'exhibits': [193, 511], 'variation': [194, 512], 'sequence.': [198, 516], 'copper-containing': [203, 521, 2013, 2027, 2758, 3567], 'protein': [204, 522, 803, 1246, 3086, 3432, 3487, 3588], 'only': [205, 523, 1480, 1623, 1730, 3815], 'shows': [207, 525], 'requirement': [209, 527], 'for': [210, 528, 1744, 2775, 3328, 3366, 3377, 3476, 3500, 3725, 3977, 4173, 4447], 'Cu(I)': [211, 529, 2488], 'ions.': [212, 530], 'Approximately': [213, 531, 3864], '12–15': [214, 532], 'Cu': [215, 533], 'ions': [216, 534, 1728, 2206, 2297, 2491, 3044, 3065, 4028], 'per': [217, 535], '94-kDa': [218, 536], 'monomeric': [219, 311, 537, 629], 'unit': [220, 538], 'observed.': [222, 540, 1874], 'sensitive': [226, 544, 3018], 'dioxygen': [228, 234, 296, 546, 552, 614, 652, 4132], 'tension.': [229, 547], 'On': [230, 548], 'basis': [232, 550, 4254], 'sensitivity,': [235, 553], 'kinetically': [237, 555], 'distinct': [238, 556, 720, 4209], 'enzyme': [242, 560, 637, 1405, 1423, 2028, 2650, 2667, 3083, 3101, 3205, 3260, 3332, 3407, 3453, 3568, 4041], 'distinguished.': [245, 563], 'slow': [247, 565, 3104], 'but': [248, 566, 1737, 3060], 'air-stable': [249, 567], 'form,': [250, 568], 'converted': [253, 571], 'into': [254, 572, 2302, 4258], '"pulsed"': [256, 574], 'state': [257, 575], 'upon': [258, 292, 576, 610, 2817, 3227], 'direct': [259, 577], 'exposure': [260, 293, 578, 611], 'atmospheric': [262, 295, 580, 613], 'oxygen': [263, 581], 'pressure,': [264, 582], 'considered': [266, 584], 'as': [267, 585, 653, 3022, 3024, 3145, 3589, 3803, 4200], 'type': [268, 586], 'I': [269, 587], 'pMMO.': [270, 588, 3039, 3941], 'This': [271, 589, 683, 1404, 2741, 3029, 3352, 4334], 'form': [272, 590, 2647, 3585], 'subject': [275, 593, 703], 'our': [277, 595, 2004, 3355], 'isolation': [279, 597, 3129, 3421, 3442, 4158], 'effort.': [280, 598], 'Other': [281, 599], '(types': [283, 601], 'II': [284, 602], 'III)': [286, 604], 'are': [287, 605, 754], 'deactivated': [288, 606], 'various': [290, 608, 2493, 3160, 3395, 3404, 3473], 'extents': [291, 609], 'pressure.': [297, 615], 'Under': [298, 616], 'inactivating': [299, 617], 'conditions,': [300, 618], 'unstable': [302, 620], 'release': [304, 622], 'protons': [305, 623], 'buffer': [308, 626, 3995, 4017, 4037, 4053, 4298, 4323, 4359], '(∼10': [309, 627], 'H+/94-kDa': [310, 628], 'unit)': [312, 630], 'eventually': [314, 632], 'become': [315, 633], 'completely': [316, 634, 4114], 'inactive.': [317, 635, 3088], 'monooxygenase,': [639], 'methanotrophic': [642], 'bacteria,': [643], 'catalyzes': [644], 'conversion': [646, 1870], 'methanol': [650], 'co-substrate': [655], 'at': [656, 758, 3056, 3516, 3534, 3790, 3947, 3955, 3973, 4090, 4169, 4181, 4433, 4438, 4444], 'ambient': [657], 'temperatures': [658], 'pressures': [660], '(1Anthony': [661, 1436], 'C.': [662, 672, 1437, 1448], 'Biochemistry': [664, 1439, 3291], 'Methylotrophs.': [666, 1441], 'Academic': [667, 1309, 1442, 2091, 2375, 2540, 2907], 'Press,': [668, 1443], 'London1982:': [669, 1444], '296-379Google': [670, 1445], 'Scholar,2Bédard': [671], 'Knowles': [673, 1449], 'R.': [674, 1450, 1707, 1710, 1849, 1852, 1986, 1989, 2181, 2466, 2631, 2997, 3643], 'Microbiol.': [675, 775, 808, 1451, 1463, 1520, 1599, 1654, 1684, 1796, 1826, 1933, 1963], 'Rev.': [676, 711, 774, 807, 1452, 1462], '1989;': [677, 1453, 3247], '53:': [678, 1454, 3296], '68-84Crossref': [679, 1455], 'PubMed': [680, 779, 812, 840, 896, 917, 958, 1005, 1021, 1037, 1050, 1197, 1268, 1355, 1456, 1467, 1544, 1563, 1584, 1603, 2050, 2191, 2228, 2245, 2334, 2476, 2641, 2866, 3007, 3185, 3284], 'Google': [681, 717, 782, 815, 843, 856, 875, 899, 920, 941, 959, 984, 1006, 1022, 1038, 1053, 1072, 1091, 1116, 1147, 1168, 1200, 1222, 1269, 1314, 1339, 1358, 1382, 1457, 1470, 1509, 1527, 1547, 1566, 1587, 1604, 1646, 1674, 1788, 1816, 1925, 1953, 2051, 2096, 2121, 2144, 2192, 2229, 2248, 2272, 2335, 2380, 2405, 2429, 2477, 2545, 2570, 2594, 2642, 2712, 2739, 2867, 2912, 2937, 2960, 3008, 3188, 3285, 3663], 'Scholar).': [682, 718, 783, 816, 1169, 1223, 1403, 1471, 1605, 1721, 1863, 2000, 2193, 2293, 2478, 2643, 2740, 3009, 3189, 3250, 3664], 'system': [684, 792, 3137], 'has': [685, 819, 1182, 1476, 2651, 2678, 2745, 2782, 3033, 3102], 'attracted': [686], 'considerable': [687], 'attention,': [688], 'since': [689], 'it': [690, 3849, 4071], 'provides': [691], 'ideal': [693], 'natural': [694], 'model': [695, 2750], 'activation': [699, 2777], 'functionalization,': [701], 'significant': [705, 3116], 'current': [706], 'interest': [707], '(3Crabtree': [708], 'R.H.': [709], 'Chem.': [710, 868, 887, 934, 951, 977, 998, 1014, 1030, 1065, 1084, 1109, 1140, 1162, 1212, 1261, 1332, 2043, 2114, 2221, 2327, 2398, 2563, 2705, 2732, 2859, 2930], '1995;': [712, 888, 1111, 1163, 1214, 1352, 2188, 2242, 2473, 2638, 3004], '95:': [713], '987-1007Crossref': [714], 'Scopus': [715, 780, 813, 841, 854, 873, 897, 918, 939, 982, 1051, 1070, 1089, 1114, 1145, 1166, 1198, 1220, 1337, 1356, 1468, 1507, 1525, 1545, 1564, 1585, 1644, 1672, 1786, 1814, 1923, 1951, 2119, 2246, 2403, 2568, 2710, 2737, 2935, 3186], '(793)': [716], 'Two': [719], '(MMO)': [725], '1The': [726], 'abbreviations': [727], 'used': [728, 3598, 3737, 4127, 4199], 'are:': [729], 'MMO,': [730], 'monooxygenase;': [732, 736, 740, 746], 'sMMO,': [733], 'soluble': [734, 785, 4349], 'pMMOH,': [741], 'hydroxylase;': [743], 'AMO,': [744], 'ammonia': [745], 'Pipes,': [747, 4302, 4367, 4386], 'piperazine-N,N′-bis(2-ethanesulfonic': [748], 'acid);': [749], 'PAGE,': [750], 'polyacrylamide': [751], 'gel': [752], 'electrophoresis.': [753], 'exist': [757], 'different': [759], 'cellular': [760], 'locations,': [761], 'cytoplasmic': [763], '(soluble)': [764], 'MMO': [765, 770, 786], 'membrane-bound': [768, 1766, 2204, 3498, 4154], '(particulate)': [769], '(4Lipscomb': [771, 804], 'J.D.': [772, 805, 834, 865, 884, 908, 929, 948, 972, 995, 1342, 1460, 2167, 2232, 2452, 2617, 2698, 2983], 'Annu.': [773, 806, 1461], '1994;': [776, 809, 914, 1262, 1464, 2044, 2222, 2328, 2860], '48:': [777, 810, 1465], '371-399Crossref': [778, 811, 1466], '(339)': [781, 814, 1469], '(sMMO)': [787], 'complex': [790], 'three-component': [791], 'consisting': [793], 'hydroxylase,': [796], 'reductase,': [798], 'small': [801, 3730], 'regulatory': [802], 'sMMO': [818, 1176, 1475, 1613, 1617, 1902], 'been': [820, 1183, 1477, 1739, 2652, 2783, 3103], 'investigated': [821], 'extensively': [822], 'several': [824, 2826, 3548], 'research': [825], 'groups': [826], '(5Fox': [827], 'B.G.': [828, 863, 906, 927, 962], 'Froland': [829, 924, 969, 2695], 'W.A.': [830, 925, 970, 987, 2696], 'Jollie': [831], 'D.R.': [832], 'Lipscomb': [833, 864, 883, 907, 928, 947, 971, 994, 2697], 'Methods': [835, 849, 1502, 3436], 'Enzymol.': [836, 850, 1503], '1990;': [837, 851, 870, 1504, 1522, 1541], '188:': [838, 852, 1505], '191-202Crossref': [839], '(97)': [842], 'Scholar,': [844, 857, 876, 900, 921, 942, 960, 985, 1007, 1023, 1039, 1054, 1073, 1092, 1117, 1148, 1201, 1270, 1315, 1340, 1359, 1383, 1446, 1458, 1510, 1528, 1548, 1567, 1588, 1647, 1658, 1675, 1688, 1789, 1800, 1817, 1830, 1926, 1937, 1954, 1967, 2052, 2097, 2145, 2165, 2230, 2249, 2273, 2336, 2381, 2406, 2430, 2450, 2546, 2571, 2595, 2615, 2713, 2868, 2913, 2961, 2981, 3286], '6Pilkington': [845], 'S.J.': [846, 1060, 1081, 1106, 1137, 1158, 1190, 1211, 1252, 1276, 1323, 1499, 2034, 2058, 2105, 2212, 2318, 2342, 2389, 2507, 2554, 2850, 2874, 2921], 'Dalton': [847, 1010, 1026, 1042, 1132, 1500, 1515, 1535, 1650, 1665, 1792, 1807, 1929, 1944, 3242, 3644], 'H.': [848, 1011, 1027, 1043, 1133, 1288, 1501, 1516, 1536, 1552, 1651, 1666, 1793, 1808, 1930, 1945, 2070, 2354, 2519, 2702, 2723, 2886, 3243, 3645], '181-190Crossref': [853, 1506], '(63)': [855, 1508], '7Hendrich': [858], 'M.P.': [859, 964, 3647], 'Münck': [860, 909, 973], 'E.': [861, 910, 974, 2177, 2462, 2627, 2993], 'Fox': [862, 905, 926], 'J.': [866, 885, 932, 949, 975, 996, 1009, 1012, 1025, 1028, 1041, 1045, 1063, 1082, 1107, 1127, 1138, 1259, 1330, 1349, 1630, 1652, 1679, 1682, 1699, 1772, 1794, 1821, 1824, 1841, 1909, 1931, 1958, 1961, 1978, 2041, 2112, 2171, 2175, 2186, 2219, 2239, 2325, 2396, 2456, 2460, 2471, 2561, 2621, 2625, 2636, 2703, 2730, 2857, 2928, 2987, 2991, 3002, 3181, 3245, 3279], 'Am.': [867, 933, 976, 1064, 1083, 1108, 1139, 1331, 2113, 2397, 2562, 2704, 2731, 2929], 'Soc.': [869, 935, 978, 1066, 1085, 1110, 1141, 1333, 2115, 2399, 2564, 2706, 2733, 2931], '112:': [871], '5861-5865Crossref': [872], '(143)': [874], '8Liu': [877], 'Y.': [878, 904, 993], 'Nesheim': [879, 945], 'J.C.': [880, 946, 1393, 1518, 1538, 1573, 2155, 2183, 2283, 2440, 2468, 2605, 2633, 2971, 2999], 'Lee': [881, 990], 'S.K.': [882, 944], 'Biol.': [886, 950, 997, 1013, 1029, 1213, 1260, 2042, 2220, 2326, 2858], '270:': [889], '24662-24665Abstract': [890], 'Full': [891, 893, 955, 1002, 1018, 1034, 1217, 1265, 2047, 2225, 2331, 2863], 'Text': [892, 894, 956, 1003, 1019, 1035, 1218, 1266, 2048, 2226, 2332, 2864], 'PDF': [895, 957, 1004, 1020, 1036, 1219, 1267, 2049, 2227, 2333, 2865], '(151)': [898], '9Paulsen': [901], 'K.E.': [902, 1058, 1075, 1094], 'Liu': [903, 992, 1057], 'Stankovich': [911], 'M.T.': [912], 'Biochemistry.': [913], '33:': [915], '713-722Crossref': [916], '(99)': [919], '10Pulver': [922], 'S.': [923, 1129, 1692, 1698, 1834, 1840, 1971, 1977], 'Solomon': [930], 'E.I.': [931], '1993;': [936, 952, 979, 1086, 1194], '115:': [937, 980, 1087], '12409-12422Crossref': [938], '(86)': [940], '11Lee': [943], '268:': [953], '21569-21577Abstract': [954], '12Fox': [961], 'Hendrich': [963], 'Surerus': [965], 'K.K.': [966, 968, 989], 'Andersson': [967, 988], '3688-3701Crossref': [981], '(175)': [983], '13Froland': [986], 'S.-K.': [991], '1992;': [999, 1560, 2707], '267:': [1000], '17588-17597Abstract': [1001], '14Green': [1008], '1988;': [1015, 1581, 3295], '263:': [1016], '17561-17565Abstract': [1017], '15Green': [1024], '1985;': [1031, 1047, 1641, 1655, 1783, 1797, 1920, 1934], '260:': [1032], '15795-15801Abstract': [1033], '16Lund': [1040], 'Eur.': [1044, 3244], 'Biochem.': [1046, 1351, 1559, 2241, 3180, 3246], '147:': [1048], '291-296Crossref': [1049], '(105)': [1052], '17DeRose': [1055], 'V.J.': [1056], 'Lippard': [1059, 1080, 1105, 1136, 1157, 1189, 1210], 'Hoffman': [1061], 'B.M.': [1062], '1996;': [1067, 1334, 2116, 2400, 2565, 2734, 2932, 3281], '117:': [1068, 1112], '121-134Crossref': [1069], '(73)': [1071], '18Liu': [1074], 'Johnson': [1076], 'C.C.': [1077], 'Newcomb': [1078], 'M.': [1079, 1274, 1895, 2056, 2340, 2505, 2717, 2872, 3131, 3217, 3595, 3942], '939-947Crossref': [1088], '(214)': [1090], '19Liu': [1093], 'Valentine': [1095], 'A.M.': [1096, 1282, 2064, 2348, 2513, 2880], 'Wang': [1097], 'D.L.': [1098], 'Huynh': [1099], 'B.H.': [1100], 'Edmondson': [1101], 'D.E.': [1102], 'Salifoglou': [1103, 1153], 'A.': [1104, 1154, 1596, 1628, 1770, 1907, 2169, 2173, 2454, 2458, 2619, 2623, 2985, 2989, 3651], '10174-10185Crossref': [1113], '(343)': [1115], '20Dewitt': [1118], 'J.G.': [1119, 1121, 1150], 'Bentsen': [1120], 'Rosenzweig': [1122, 1151], 'A.C.': [1123, 1152, 1186, 1203, 1512, 1530], 'Hedman': [1124, 1155, 1279, 1320, 2061, 2102, 2345, 2386, 2510, 2551, 2877, 2918], 'B.': [1125, 1156, 1280, 1286, 1321, 2062, 2068, 2103, 2346, 2352, 2387, 2511, 2517, 2552, 2715, 2878, 2884, 2919], 'Green': [1126], 'Pilkington': [1128], 'Papaefthymiou': [1130], 'G.C.': [1131], 'Hodgson': [1134, 1159, 1295, 1326, 2077, 2108, 2361, 2392, 2526, 2557, 2893, 2924], 'K.O.': [1135, 1160, 1296, 1327, 2078, 2109, 2362, 2393, 2527, 2558, 2894, 2925], '1991;': [1142], '113:': [1143], '9219-9235Crossref': [1144], '(223)': [1146], '21Dewitt': [1149], 'Inorg.': [1161, 1350, 2240], '34:': [1164], '2505-2515Crossref': [1165], '(46)': [1167, 1586], 'x-ray': [1171], 'crystal': [1172], 'structure': [1173], 'hydroxylase': [1177, 1225], 'fromMethylococcus': [1179], 'solved': [1184], '(22Rosenzweig': [1185], 'Frederick': [1187, 1208], 'C.A.': [1188, 1209], 'Nordlund': [1191, 1204], 'P.': [1192, 1205], 'Nature.': [1193], '366:': [1195], '537-543Crossref': [1196], '(861)': [1199], '23Rosenzweig': [1202], 'Takahara': [1206], 'P.M.': [1207], '2:': [1215], '409-418Abstract': [1216], '(431)': [1221], 'site': [1227, 2760, 3307], 'contains': [1228], 'non-heme': [1230], 'binuclear': [1231], 'iron': [1232, 3313, 3343], 'cluster.': [1233], 'In': [1234, 1606, 2825, 3190], 'contrast,': [1235], 'appears': [1241, 1429], '(24Nguyen': [1247, 2029, 2207, 2313, 2845], 'H.-H.T.': [1248, 1317, 1363, 1387, 2030, 2099, 2125, 2149, 2208, 2253, 2277, 2314, 2383, 2410, 2434, 2548, 2575, 2599, 2721, 2846, 2915, 2941, 2965], 'Shiemke': [1249, 1364, 1388, 2031, 2126, 2150, 2209, 2254, 2278, 2315, 2411, 2435, 2576, 2600, 2847, 2942, 2966], 'A.K.': [1250, 1365, 1389, 2032, 2127, 2151, 2210, 2255, 2279, 2316, 2412, 2436, 2577, 2601, 2848, 2943, 2967], 'Jacobs': [1251, 2033, 2211, 2317, 2849], 'Hales': [1253, 2035, 2213, 2319, 2851], 'B.J.': [1254, 2036, 2214, 2320, 2852], 'Lidstrom': [1255, 1293, 1299, 1324, 1345, 1366, 1390, 2037, 2075, 2081, 2106, 2128, 2152, 2184, 2215, 2235, 2256, 2280, 2321, 2359, 2365, 2390, 2413, 2437, 2469, 2524, 2530, 2555, 2578, 2602, 2634, 2853, 2891, 2897, 2922, 2944, 2968, 3000], 'M.E.': [1256, 1294, 1300, 1325, 1346, 1367, 1391, 2038, 2076, 2082, 2107, 2129, 2153, 2185, 2216, 2236, 2257, 2281, 2322, 2360, 2366, 2391, 2414, 2438, 2470, 2525, 2531, 2556, 2579, 2603, 2635, 2854, 2892, 2898, 2923, 2945, 2969, 3001], 'Chan': [1257, 1297, 1328, 1347, 2039, 2079, 2110, 2217, 2237, 2323, 2363, 2394, 2528, 2559, 2726, 2855, 2895, 2926], 'S.I.': [1258, 1298, 1329, 1348, 1361, 1385, 2040, 2080, 2111, 2123, 2147, 2218, 2238, 2251, 2275, 2324, 2364, 2395, 2408, 2432, 2529, 2560, 2573, 2597, 2727, 2856, 2896, 2927, 2939, 2963], '269:': [1263, 2045, 2223, 2329, 2861], '14995-15005Abstract': [1264, 2046, 2224, 2330, 2862], '25Nguyen': [1271, 2053, 2337, 2869], 'H.-H.N.T.': [1272, 2054, 2338, 2503, 2870], 'Zhu': [1273, 2055, 2339, 2504, 2716, 2871], 'Elliott': [1275, 1322, 2057, 2104, 2341, 2388, 2506, 2553, 2873, 2920], 'Nakagawa': [1277, 1318, 2059, 2100, 2343, 2384, 2508, 2549, 2875, 2916], 'K.H.': [1278, 1319, 2060, 2101, 2344, 2385, 2509, 2550, 2876, 2917], 'Costello': [1281, 2063, 2172, 2347, 2457, 2512, 2622, 2879, 2988], 'Peeples': [1283, 2065, 2349, 2514, 2881], 'T.L.': [1284, 2066, 2350, 2515, 2882], 'Wilkinson': [1285, 2067, 2351, 2516, 2883], 'Morimoto': [1287, 2069, 2353, 2518, 2701, 2722, 2885], 'Williams': [1289, 2071, 2355, 2520, 2699, 2724, 2887], 'P.G.': [1290, 2072, 2356, 2521, 2700, 2725, 2888], 'Floss': [1291, 2073, 2357, 2522, 2693, 2728, 2889], 'H.G.': [1292, 2074, 2358, 2523, 2694, 2729, 2890, 3649, 3653], 'Tabita': [1301, 2083, 2367, 2532, 2899], 'F.R.': [1302, 2084, 2368, 2533, 2900], 'Microbial': [1303, 1396, 1701, 1843, 1980, 2085, 2158, 2286, 2369, 2443, 2534, 2608, 2901, 2974], 'Growth': [1304, 1397, 1702, 1844, 1981, 2086, 2159, 2287, 2370, 2444, 2535, 2609, 2902, 2975], 'on': [1305, 1398, 1703, 1845, 1982, 2087, 2160, 2288, 2371, 2445, 2496, 2536, 2610, 2903, 2976, 3383, 3604, 4252], 'C1': [1306, 1399, 1704, 1846, 1983, 2088, 2161, 2289, 2372, 2446, 2537, 2611, 2904, 2977], 'Compounds.': [1307, 1400, 2089, 2162, 2290, 2373, 2447, 2538, 2612, 2905, 2978], 'Kluwer': [1308, 2090, 2374, 2539, 2906], 'Publishers,': [1310, 2092, 2376, 2541, 2908], 'Norwell,': [1311, 2093, 2377, 2542, 2909], 'MA1996:': [1312, 2094, 2378, 2543, 2910], '150-158Crossref': [1313, 2095, 2379, 2544, 2911], '26Nguyen': [1316, 2098, 2382, 2547, 2914], '118:': [1335, 2117, 2401, 2566, 2735, 2933], '12766-12776Crossref': [1336, 2118, 2402, 2567, 2934], '(114)': [1338, 2120, 2404, 2569, 2936], '27Semrau': [1341, 2231], 'Zolandz': [1343, 2233], 'D.': [1344, 1636, 1677, 1695, 1778, 1819, 1837, 1915, 1956, 1974, 2234, 3239, 3241], '58:': [1353, 2243], '235-244Crossref': [1354, 2244], '(60)': [1357, 2247], '28Chan': [1360, 2250, 2407, 2572], 'Nguyen': [1362, 1386, 2124, 2148, 2252, 2276, 2409, 2433, 2574, 2598, 2720, 2940, 2964], 'Karlin': [1368, 2130, 2258, 2415, 2580, 2946], 'K.D.': [1369, 2131, 2259, 2416, 2581, 2947], 'Tyeklar': [1370, 2132, 2260, 2417, 2582, 2948], 'Z.': [1371, 2133, 2261, 2418, 2583, 2949], 'Bioinorganic': [1372, 2134, 2262, 2419, 2584, 2950], 'Chemistry': [1373, 2135, 2263, 2420, 2585, 2951], 'Copper.': [1375, 2137, 2265, 2422, 2587, 2953], 'Chapman': [1376, 2138, 2266, 2423, 2588, 2954], 'Hall,': [1378, 2140, 2268, 2425, 2590, 2956], 'New': [1379, 2141, 2269, 2426, 2591, 2957, 3657, 3660], 'York1993:': [1380, 2142, 2270, 2427, 2592, 2958], '184-195Crossref': [1381, 2143, 2271, 2428, 2593, 2959], '29Chan': [1384, 2146, 2274, 2431, 2596, 2962], 'Murrell': [1392, 1517, 1537, 2154, 2182, 2282, 2439, 2467, 2604, 2632, 2970, 2998], 'Kelly': [1394, 2156, 2284, 2441, 2606, 2972], 'D.P.': [1395, 2157, 2285, 2442, 2607, 2973], '1993:': [1401, 2163, 2291, 2448, 2613, 2979], '93-107Google': [1402, 2164, 2292, 2449, 2614, 2980], 'much': [1407], 'less': [1408, 3812], 'well': [1409, 3023], 'characterized': [1410], 'instability.': [1417], 'Despite': [1418], 'lability': [1420], 'vitro,': [1426], 'expressed': [1432, 1619], 'all': [1434], 'methanotrophs': [1435], '2Bédard': [1447], '4Lipscomb': [1459], 'So': [1472], 'far,': [1473], 'detected': [1478], 'following': [1482, 3674], 'strains': [1483, 1607], 'species:M.': [1485], 'capsulatus,': [1486], 'Methylosinus': [1487, 1489], 'trichosporium,': [1488], 'sporium,': [1490], 'Methylocystis': [1491], 'sp.': [1492], 'M': [1493], 'Methylomonas': [1495], 'methanica': [1496], '68–1': [1497], '(6Pilkington': [1498], '30Stainthorpe': [1511], 'Salmond': [1513, 1533], 'G.P.C.': [1514, 1534], 'FEMS': [1519], 'Lett.': [1521, 1640, 1668, 1782, 1810, 1919, 1947], '70:': [1523], '103-108Crossref': [1524], '(33)': [1526], '31Stainthorpe': [1529], 'Lees': [1531], 'V.': [1532], 'Gene': [1539], '(Amst.).': [1540], '91:': [1542], '27-34Crossref': [1543], '(139)': [1546], '32Nakajima': [1549], 'T.': [1550, 1556], 'Uchiyama': [1551], 'Yagi': [1553], 'O.': [1554], 'Nakahara': [1555], 'Biosci.': [1557], 'Biotech.': [1558], '56:': [1561], '736-740Crossref': [1562], '(39)': [1565], '33Prince': [1568], 'R.C.': [1569], 'George': [1570], 'G.N.': [1571], 'Savas': [1572], 'Crammer': [1574], 'S.P.': [1575], 'Patel': [1576], 'R.N.': [1577, 1590], 'Biochim.': [1578], 'Biophys.': [1579], 'Acta.': [1580], '952:': [1582], '220-229Crossref': [1583], '34Patel': [1589], 'Hou': [1591], 'C.T.': [1592], 'Laskin': [1593], 'A.J.': [1594, 2179, 2464, 2629, 2995], 'Pelix': [1595], 'Appl.': [1597], 'Environ.': [1598], '1982;': [1600], '44:': [1601], '1130-1137Crossref': [1602], 'capable': [1608], 'expressing': [1610, 1900], 'either': [1611], 'or': [1614, 2658, 2835, 3521, 3561, 3578, 4113, 4232, 4378, 4436], '(low': [1624, 4360], 'copper/biomass': [1625], 'ratio)': [1626], '(35Cornish': [1627, 1769, 1906], 'MacDonald': [1629, 1771, 1908], 'Burrows': [1631, 1773, 1910], 'K.J.': [1632, 1774, 1911], 'King': [1633, 1775, 1912], 'T.S.': [1634, 1776, 1913], 'Scott': [1635, 1777, 1914], 'Higgins': [1637, 1680, 1779, 1822, 1916, 1959, 3178], 'I.J.': [1638, 1681, 1780, 1823, 1917, 1960, 3179], 'Biotechnol.': [1639, 1667, 1781, 1809, 1918, 1946], '5:': [1642, 1670, 1784, 1812, 1921, 1949], '319-324Crossref': [1643, 1785, 1922], '(20)': [1645, 1787, 1924], '36Prior': [1648, 1790, 1927], 'S.D.': [1649, 1662, 1791, 1804, 1928, 1941], 'Gen.': [1653, 1683, 1795, 1825, 1932, 1962], '131:': [1656, 1798, 1935], '155-163Google': [1657, 1799, 1936], '37Stanley': [1659, 1801, 1938], 'S.H.': [1660, 1802, 1939], 'Prior': [1661, 1803, 1940], 'Leak': [1663, 1805, 1942], 'D.J.': [1664, 1806, 1943], '1983;': [1669, 1811, 1948], '487-492Crossref': [1671, 1813, 1950], '(316)': [1673, 1815, 1952], '38Scott': [1676, 1818, 1955], 'Brannan': [1678, 1820, 1957], '1981;': [1685, 1827, 1964], '125:': [1686, 1828, 1965], '63-72Google': [1687, 1829, 1966], '39Dalton,': [1689, 1831, 1968], 'H.,': [1690, 1700, 1832, 1842, 1969, 1979], 'Prior,': [1691, 1833, 1970], 'D.,': [1693, 1835, 1972], 'Leak,': [1694, 1836, 1973], 'J.,': [1696, 1838, 1975], 'Stanley,': [1697, 1839, 1976], 'Compounds,': [1705, 1847, 1984], 'Crawford,': [1706, 1848, 1985], 'L.,': [1708, 1850, 1987], 'Hanson,': [1709, 1851, 1988], 'S.,': [1711, 1853, 1990], '1984,': [1712, 1854, 1991], '75,': [1713, 1855, 1992], '82,': [1714, 1856, 1993], '.,': [1715, 1857, 1994], 'Intercept,': [1716, 1858, 1995], 'Andover,': [1717, 1859, 1996], 'Hampshire,': [1718, 1860, 1997], 'United': [1719, 1861, 1998], 'Kingdom.Google': [1720, 1862, 1999], 'Otherwise,': [1722], 'expressed.': [1726], 'Copper': [1727], 'not': [1729, 3073, 3198, 3562, 3579, 3930, 4275], 'regulate': [1731], 'expression': [1733, 1748], 'have': [1738, 3113, 3381, 4283], 'crucial': [1743], 'activity.': [1746, 3267, 3863], 'accompanied': [1753], 'formation': [1756], 'extensive': [1759, 3385], 'network': [1760], 'intracytoplasmic': [1762, 3835, 4235], 'where': [1764], 'resides': [1768], 'An': [1864, 3124], 'increase': [1865, 3921], 'carbon': [1867], 'biomass': [1869], 'efficiency': [1871], 'also': [1873, 2669, 3013, 3838, 3850, 4032, 4415], 'Three': [1875], 'new': [1876, 2767], 'polypeptides': [1877], 'masses': [1881], '26': [1886], 'kDa': [1887], 'observed': [1889, 2831, 3237], 'fractions': [1893], 'when': [1894], 'switched': [1898], 'Recent': [2001], 'progress': [2002], 'laboratory': [2005], 'indicates': [2006, 3134], 'that': [2007, 2021, 3135, 3304, 3322, 3413, 3446, 3766, 4122, 4454], 'novel': [2012], 'enzyme.': [2014, 2812, 3123], 'Metal/protein': [2015], 'ratio': [2016, 3776], 'data': [2017], 'analysis': [2018, 4065], 'clearly': [2019], 'suggests': [2020], 'multiple': [2026], 'Scholar,28Chan': [2122, 2938], '40Semrau': [2166, 2451, 2616, 2982], 'Chistoserdov': [2168, 2453, 2618, 2984], 'Lebron': [2170, 2455, 2620, 2986], 'Davagnino': [2174, 2459, 2624, 2990], 'Kenna': [2176, 2461, 2626, 2992], 'Holmes': [2178, 2463, 2628, 2994], 'Finch': [2180, 2465, 2630, 2996], 'Bacteriol.': [2187, 2472, 2637, 3003, 3280], '177:': [2189, 2474, 2639, 3005], '3071-3079Crossref': [2190, 2475, 2640, 3006], 'Activity': [2194, 2813], 'proportional': [2199], 'level': [2202], 'pMMO-associated': [2295], 'appear': [2298, 3299, 4281], 'organized': [2301], 'trinuclear': [2303], 'cluster': [2304], 'units': [2305], 'rather': [2307, 4058], 'defined': [2308], 'magnetic': [2309], 'redox': [2311], 'properties': [2312], 'as-isolated': [2480], 'pMMO-enriched': [2481], 'often': [2483, 4049, 4108, 4207], 'mixture': [2486], 'Cu(II)': [2490, 4027], 'proportions,': [2494], 'depending': [2495], 'handling': [2498], 'samples': [2501], '(25Nguyen': [2502], 'Hence,': [2644], 'functional': [2646], 'suggested': [2653, 3590], 'reduced': [2657, 2660], 'partially': [2659], 'form.': [2661], 'chemistry': [2663, 2743], 'catalyzed': [2664], 'this': [2666, 3136, 3543, 3825, 3925, 4036], 'specific.': [2671], 'pMMO-catalyzed': [2672], 'hydroxylation': [2673], 'cryptically': [2675], 'chiral': [2676], 'ethanes': [2677], 'implicated': [2679], 'reaction': [2681], 'mechanism': [2682], 'proceeding': [2683], 'complete': [2685, 3208, 3264], 'retention': [2686], 'alkane': [2688], 'substrate': [2689], 'configuration': [2690], '(41Priestly': [2691], 'N.D.': [2692, 2719], '114:': [2708], '7561-7562Crossref': [2709], '(153)': [2711], '42Wilkinson': [2714], 'Priestley': [2718], '921-922Crossref': [2736], '(89)': [2738], 'extraordinary': [2742], 'currently': [2744, 3078], 'no': [2746, 2828], 'precedent': [2747], 'biological': [2752], 'systems.': [2753], 'Accordingly,': [2754], 'insights': [2755], 'regarding': [2756, 3118], 'provide': [2765], 'direction': [2768], 'design': [2771], 'biominetic': [2773], 'catalysts': [2774], 'functionalization.': [2779], 'study.': [2790], 'As': [2791, 3089], 'noted': [2792, 4453], 'earlier,': [2793], 'one': [2794, 3584], 'main': [2797], 'obstacles': [2798], 'studying': [2800], 'frequently': [2815], 'lost': [2816], 'cell': [2818, 2843, 3780, 3874, 3902, 3922, 4025, 4089, 4102, 4125, 4163, 4332, 4408], 'lysis,': [2819], 'detergent': [2820, 3223, 3228], 'solubilization,': [2821], 'freeze-thaw': [2823, 3529], 'cycles.': [2824], 'cultures,': [2827], 'cell-free': [2833, 4116], 'extracts,': [2834], 'quickly': [2837, 4059], 'disappeared': [2838], 'within': [2839, 3330], '6': [2840, 3870], 'h': [2841, 3727, 3866, 3871, 3896, 3899, 3968], 'after': [2842, 3867, 3969, 4196], 'lysis': [2844, 3994], 'Enzymatic': [3010], 'exogenous': [3020], 'ligands': [3021], 'choice': [3026], 'buffer.': [3028], 'hampered': [3034], 'efforts': [3035, 3108, 3356], 'characterizing': [3037], 'addition': [3041, 3142, 4046], 'enhance': [3048], 'enzymatic': [3049, 3391], 'certain': [3052], '(cells': [3054], 'grown': [3055, 3670, 3946], 'low': [3057, 4111], 'levels),': [3059], 'effect': [3062], 'extension': [3068], 'known.': [3074], 'No': [3075], 'reagent': [3076], 'reactivate': [3081], 'once': [3084], 'becomes': [3087], 'result,': [3091], 'active,': [3094], 'stable,': [3095], 'purified': [3097, 3449, 4068], 'preparation': [3098], 'forthcoming.': [3106], 'Past': [3107], 'isolating': [3110], 'resulted': [3114, 3206, 3262, 4141], 'confusion': [3117], 'nature': [3120, 3555], 'early': [3125], 'report': [3126], 'trichosporium': [3132], 'OB3b': [3133], 'utilize': [3139], 'ascorbate': [3140], 'NADH': [3144], 'electron': [3146], 'donors': [3147], 'components:': [3155], '47-kDa': [3157], 'polypeptide': [3158], 'containing': [3159, 3607, 3693, 3742, 3996, 4215, 4299], 'amounts': [3161], 'copper,': [3163], 'smaller': [3165], '9.4-kDa': [3166], '13-kDa': [3170], 'CO-binding': [3171], 'cytochrome': [3172], 'c': [3173], '(43Tonge': [3174], 'G.M.': [3175], 'Harrison': [3176], 'D.E.F.': [3177], '1977;': [3182], '161:': [3183], '333-344Crossref': [3184], '(83)': [3187], 'later': [3191], 'studies,': [3192], 'aforementioned': [3194], 'ascorbate-linked': [3195], 'observed,': [3199], 'attempts': [3201, 3256], 'solubilize': [3203], 'deactivation': [3209], 'protein.': [3212], 'Solubilization': [3213], 'nonionic': [3222], 'attempted,': [3225], 'removal': [3229], 'lipid': [3231], 'vesicle': [3232], 'reconstitution,': [3233], 'partial': [3234], '(44Drummond': [3238], 'Smith': [3240], '1982:': [3248], '667-671Google': [3249], 'According': [3251], 'authors,': [3254], 'any': [3255], 'purify': [3258, 3360], 'further': [3261, 4251], 'loss': [3265], 'few': [3269, 4339], 'reports': [3270], 'including': [3271, 3394], 'recent': [3273, 3592], 'work': [3274], '(45Zahn': [3275], 'J.A.': [3276], 'DiSpirito': [3277], 'A.A.': [3278], '178:': [3282], '1018-1029Crossref': [3283], "46Akent'eva": [3287], 'N.F.': [3288], 'Gvozdev': [3289], 'R.I.': [3290], '(Engl.': [3292], 'Transl.': [3293], 'Biokhimiya).': [3294], '91-96Google': [3297], 'Scholar)': [3298], 'support': [3301], 'notion': [3303], 'may': [3311], 'despite': [3314], 'overwhelming': [3316], 'evidence': [3317], 'accumulated': [3318], 'date': [3320], 'suggesting': [3321], 'element': [3326], 'responsible': [3327], 'catalysis': [3329], 'site.': [3334], 'Thus,': [3335], 'advance': [3337], 'field,': [3339], 'presence': [3341], 'must': [3349, 3952], 'resolved.': [3351], 'paper': [3353], 'summarizes': [3354], 'isolate': [3358], 'fromM.': [3363], 'biochemical': [3367], 'biophysical': [3369], 'characterization.': [3370], 'Toward': [3371], 'development': [3373], 'suitable': [3375], 'isolation,': [3379, 3402], 'we': [3380, 3545], 'embarked': [3382], 'investigation': [3386], 'factors': [3388], 'contributing': [3389], 'stability,': [3393], 'methods': [3396, 3422, 3485], 'bacterial': [3398, 3418, 3438], 'cultivation': [3399, 3419, 3439], 'schemes': [3405], 'stabilization': [3408], 'purification.': [3410, 3488], 'We': [3411], 'find': [3412], 'details': [3415], 'significantly': [3423], 'affect': [3424], 'quality': [3426], 'them.': [3435], 'optimized': [3444], 'such': [3445, 3490, 3765], 'could': [3454], 'recovered.': [3456], 'Active': [3457], 'fractions,': [3459, 4243], 'exhibiting': [3465], 'activity,': [3468, 3477], 'procedures,': [3474], 'assayed': [3475], 'solubilized': [3478], 'detergents,': [3480], 'fractionated': [3482], 'available': [3484], 'For': [3489], 'preparations,': [3491], 'extended': [3502], 'period': [3503], 'time,': [3505], 'minimum': [3507], '3–4': [3509], 'days': [3510, 3515], 'up': [3512], '10': [3514], '4': [3517, 3999, 4434], '°C': [3518, 4435, 4446], 'enhanced': [3522], '(stable': [3524], 'respect': [3526], 'repeated': [3528, 4337], 'cycles': [3530], 'prolonged': [3532], 'storage': [3533, 4358, 4363, 4382], '−80': [3535, 4445], '°C).': [3536], 'Aside': [3537], 'describing': [3539], 'procedures': [3541], 'report,': [3544], 'will': [3546], 'discuss': [3547], 'other': [3549], 'critical': [3550], 'issues': [3551], 'relating': [3552], 'particularly': [3559], 'whether': [3560, 3577], 'only,': [3569], 'subunit': [3571], 'composition': [3572], 'there': [3580], 'more': [3582, 4340], 'than': [3583], 'genetic': [3593], 'data.': [3594], 'studies': [3601], 'Petri': [3605, 3682], 'plates': [3606, 3638, 3683], 'nitrate': [3609, 3701], 'mineral': [3610, 3702], 'salts': [3611, 3703], 'medium': [3612, 3704, 3749], 'added': [3614, 3706, 3751, 3881, 3886, 4034, 4417], 'CuSO4': [3615, 3707, 3754, 3877], '(20': [3616], 'μm)': [3617], 'solidified': [3619], '1.7%': [3621], 'agar.': [3622], 'Cultures': [3623], 'atmosphere': [3628], '20%': [3630, 3711], 'air': [3633, 3714], 'streaked': [3635], 'onto': [3636], 'fresh': [3637], 'every': [3639], '4–6': [3640], 'weeks': [3641], '(47Whittenbury': [3642], 'Starr': [3646], 'Truper': [3648], 'Balows': [3650], 'Schlegel': [3652], 'Prokaryotes.': [3655], 'Springer-Verlag': [3656], 'York,': [3658], 'Inc.,': [3659], 'York1981:': [3661], '894-902Crossref': [3662], 'Chemostat': [3665], 'cultures': [3666, 3735], '(9–10': [3667], 'liters)': [3668], 'according': [3671], 'procedure.': [3675], 'organisms': [3677, 3720], 'first': [3679], 'transferred': [3680], '250-ml': [3685], 'flasks': [3686], '2-liter': [3690], 'Erlenmeyer': [3691], 'flasks,': [3692], '40': [3694, 4174], '300': [3696], 'ml,': [3697], 'respectively,': [3698], '(10': [3708], 'μm),': [3709], 'atmosphere,': [3715], 'continual': [3717], 'shaking.': [3718], 'allowed': [3722], 'grow': [3724], '48': [3726], 'scale': [3731], 'cultures.': [3732], '300-ml': [3734], 'seed': [3739], 'fermentor': [3741], '9': [3743], 'liters': [3744], 'above': [3747, 4420], 'described': [3748], '20': [3752, 3756], 'μm': [3753, 3757], 'CuEDTA.': [3758], 'feeding': [3761, 3794, 3800, 3906], 'rate': [3762, 3771, 3907], 'controlled': [3764], 'growth-limiting': [3769], '(feeding': [3770], '∼0.01–0.012': [3772], 'feet3/h·liter).': [3773], 'methane/air': [3775], '1:4.': [3778], 'density': [3781], '>10': [3783], 'g/liter': [3784], 'culture': [3786], 'higher': [3792, 4284], 'rate.': [3795], 'However,': [3796, 3824, 4044], 'employed': [3798], 'rate,': [3801], 'termed': [3802], 'condition': [3806, 3826, 3918], '(semistarvation': [3807], 'condition),': [3809], 'results': [3810], 'biomass;': [3813], 'typically': [3814], '∼5–6': [3816], 'g': [3817, 3976, 4012, 4078, 4105, 4172], 'wet': [3819, 4014], 'cells/liter': [3820], 'would': [3821, 4061], 'obtained.': [3823], 'stimulate': [3830], 'overproduction': [3832], '(see': [3846], 'below).': [3847], 'Furthermore,': [3848], 'stimulates': [3851], 'uptake': [3853], '(high': [3854], 'copper/protein': [3855], 'ratio),': [3856], 'resulting': [3857], 'specific': [3862], '24': [3865], 'inoculation': [3868], 'prior': [3872, 3900], 'harvest,': [3875, 3903], 'additional': [3876], '(or': [3878], 'CuEDTA)': [3879], 'bring': [3883], 'concentration': [3888], '50': [3890, 3984, 3997, 4002, 4300, 4387], '60': [3892, 4011], 'μm,': [3893], 'Six': [3895], '3': [3898], 'increased': [3909], 'incrementally': [3910], '0.03–0.04': [3912], 'feet3/h·liter': [3913], 'relieve': [3915], 'starvation': [3917], '(partly': [3919], 'density).': [3923], 'Without': [3924], 'step,': [3926], 'although': [3932, 4277], 'contained': [3935], 'unusually': [3936], '42': [3948], '°C.': [3949], 'pH': [3951, 4008, 4376, 4399], '6.8–7.4': [3956], 'growth.': [3958], 'Cells': [3959], 'harvested': [3961], 'late': [3963], 'log': [3964], 'phase': [3965], '(typically': [3966, 4010], '48–52': [3967], 'inoculation)': [3970], 'centrifugation': [3972, 4168], '27,000': [3974, 4170], '×': [3975, 4171, 4183], '15': [3978], 'min': [3979, 4186], 'washed': [3981, 4292], 'twice': [3982], 'mm': [3985, 4000, 4301, 4304, 4366, 4385, 4388, 4391], 'Pipes': [3986], '(pH': [3987, 4310], '7.2).': [3988], 'Washed': [3989], 'cells': [3990, 4015, 4161], 'suspended': [3992, 4356], 'mmPipes,': [3998], 'ascorbate,': [4001, 4305, 4392], 'μg': [4003, 4307, 4371, 4394], 'catalase/ml': [4005, 4309, 4373, 4396], 'buffer,': [4007, 4364, 4375, 4383, 4398], '∼7.2': [4009], 'volume': [4020, 4328, 4403], '∼75': [4022], 'ml': [4023], 'suspension).': [4026], '(100': [4029], 'μmCuSO4)': [4030], 'improve': [4039, 4423], 'stability': [4042, 4424], 'further.': [4043, 4425], 'caused': [4050], 'ascorbate-containing': [4052], 'lose': [4055], 'effectiveness': [4057], 'complicate': [4062], 'metal': [4063], 'content': [4064], 'protein,': [4069], 'so': [4070], 'routinely': [4073], 'omitted.': [4074], 'Cell': [4075], 'suspensions': [4076, 4103, 4126, 4428], '(∼0.8–1.0': [4077], 'cells/ml)': [4080, 4107], 'passed': [4082], 'times': [4084, 4326, 4341], 'through': [4085], 'French': [4087], 'pressure': [4088], '20,000': [4091], 'p.s.i.': [4092], 'separate': [4094], 'cytosolic': [4096, 4202], 'fractions.': [4099], 'Less': [4100], 'dense': [4101, 4124], '(<0.5': [4104], 'result': [4109], 'inactive': [4115], 'extract': [4117], 'membranes.': [4119], 'It': [4120, 4450], 'appeared': [4121], 'here': [4128], '(i)': [4129], 'kept': [4130, 4432], 'tension': [4133], 'low,': [4134], 'hence': [4135], 'minimizing': [4136], 'oxidation': [4138], '(ii)': [4140], 'viscous': [4144], 'lysate,': [4145], 'helped': [4147], 'protect': [4149], 'integrity': [4151], 'process.': [4159], 'Unlysed': [4160], 'debris': [4164], 'removed': [4166], 'min.': [4175], 'supernatant': [4177, 4194, 4344], 'then': [4179, 4429], 'ultracentrifuged': [4180], '220,000': [4182], 'gfor': [4184], '90': [4185], 'pellet': [4188], 'fraction.': [4191, 4203], 'clear': [4193], 'ultracentrifugation': [4197], 'pelleted': [4205, 4353], 'show': [4208], 'layers.': [4210], 'minor': [4212], 'bottom': [4213], 'layer': [4214, 4225], 'bluish': [4216], 'black': [4218], 'materials': [4219], 'thin,': [4222], 'white': [4223], 'top': [4224], 'discarded.': [4227], 'Only': [4228], 'middle': [4230], 'layer,': [4231], 'translucent': [4234, 4289], 'bulk': [4239], 'collected.': [4245], 'These': [4246], 'separated': [4250], 'their': [4256], 'texture': [4257], '"soft"': [4259], '"hard"': [4261], 'albeit': [4263], 'difficulty.': [4265], 'difference': [4267], 'between': [4268], 'types': [4271], 'great': [4276], 'hard': [4279], 'intact': [4285], 'content.': [4287], 'suspending': [4294], 'them': [4295], 'washing': [4297, 4322], '5': [4303, 4368, 4390], '25': [4306, 4370, 4393], '7.2)': [4311], 'Dounce': [4314], 'homogenizer,': [4315], 'repelleted': [4316], 'ultracentrifugation,': [4318], 'resuspended': [4320], '2–3': [4325], 'original': [4331, 4407], 'suspension.': [4333], 'process': [4335], 'until': [4342], 'virtually': [4346], 'free': [4347], 'Finally,': [4351], 'ionic': [4361, 4380], 'strength': [4362, 4381], '20–25': [4365], 'mmascorbate,': [4369], '∼7.25;': [4377], '75–100': [4384], 'imidazole,': [4389], '∼7.25)': [4400], 'equal': [4404], 'suspension': [4409], 'volume.': [4410], 'Sucrose': [4411], '(200': [4412], 'mm)': [4413], 'buffers': [4421], 'frozen': [4437], 'liquid': [4439], 'nitrogen': [4440], 'temperature': [4441], 'stored': [4443], 'future': [4448], 'use.': [4449], 'should': [4451], 'activ': [4455]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2093768571', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}, {'year': 2023, 'cited_by_count': 4}, {'year': 2022, 'cited_by_count': 7}, {'year': 2021, 'cited_by_count': 5}, {'year': 2020, 'cited_by_count': 6}, {'year': 2019, 'cited_by_count': 7}, {'year': 2018, 'cited_by_count': 10}, {'year': 2017, 'cited_by_count': 3}, {'year': 2016, 'cited_by_count': 4}, {'year': 2015, 'cited_by_count': 2}, {'year': 2014, 'cited_by_count': 5}, {'year': 2013, 'cited_by_count': 8}, {'year': 2012, 'cited_by_count': 7}], 'updated_date': '2025-01-09T01:17:59.044453', 'created_date': '2016-06-24'}
Publication Information

Basic Information

Access and Citation

AI Researcher Chatbot

Primary Location

Authors

Topics

Keywords

Related Works
