Title: Effect of pyridoxal 5′-phosphate on the temperature relationships of alanine aminotransferase
Abstract: Details of a systematic approach to suitability testing of commercial control sera are given for substrate optimized l-aspartate aminotransferase and L-alanine aminotransferase methods at 37° C. Their acceptability for control purposes of standardized methods depends on: (1) the range of control values in relation to borderline values, (2) stability, (3) aspect, clarity, (4) NADH consumption in preincubation time, (5) blank activities, (6) kinetic data as half saturation constants and saturation curves, (7) influence of effectors, (8) isoenzyme pattern. These evaluation criteria are proposed for suitability testing. The term “representativeness” should be introduced as a special criterion for main characteristics of control materials. The authors want to point out the close connection with standardization of methods.
Publication Year: 1975
Publication Date: 1975-11-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 4
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