Title: Thin-layer chromatography as a pilot technique for rapid column chromatography
Abstract: It is proposed to utilize thin-layer chromatography for the choice of adsorbent, solvent and working conditions in column chromatography (liquid-solid chromatography), as thin-layer chromatography facilitates manipulation, is economical and, unlike column chromatography, enables numerous specific detection reactions to be used. Transfer formulae are developed whose validity is shown by several practical examples. The transfer of conditions and separations poses few problems, when single-component solvents are used. Under certain conditions (N-chamber), the use of solvent mixtures may lead to uncertainties. The activity of the plate and the column must be controlled and the water concentration of the column chromatographic solvent must be “isotonic” with the column activity. On the other hand, the column activity can be adjusted by using different water concentrations in the solvent.
Publication Year: 1972
Publication Date: 1972-05-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 40
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