Title: Growth-Promoting Nitrogen Nutrition Affects Flavonoid Biosynthesis in Young Apple (Malus domestica Borkh.) Leaves
Abstract: Plant BiologyVolume 7, Issue 6 p. 677-685 Growth-Promoting Nitrogen Nutrition Affects Flavonoid Biosynthesis in Young Apple (Malus domestica Borkh.) Leaves T. Strissel, T. Strissel Department für Pflanzenwissenschaften, Fachgebiet Obstbau, Technische Universität München-Weihenstephan, Alte Akademie 16, 85350 Freising, Germany Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, Technische Universität Wien, Getreidemarkt 9, 1060 Wien, AustriaSearch for more papers by this authorH. Halbwirth, H. Halbwirth Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, Technische Universität Wien, Getreidemarkt 9, 1060 Wien, AustriaSearch for more papers by this authorU. Hoyer, U. Hoyer Department für Pflanzenwissenschaften, Fachgebiet Obstbau, Technische Universität München-Weihenstephan, Alte Akademie 16, 85350 Freising, Germany Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, Technische Universität Wien, Getreidemarkt 9, 1060 Wien, AustriaSearch for more papers by this authorC. Zistler, C. Zistler Department für Pflanzenwissenschaften, Fachgebiet Obstbau, Technische Universität München-Weihenstephan, Alte Akademie 16, 85350 Freising, Germany Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, Technische Universität Wien, Getreidemarkt 9, 1060 Wien, AustriaSearch for more papers by this authorK. Stich, K. Stich Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, Technische Universität Wien, Getreidemarkt 9, 1060 Wien, AustriaSearch for more papers by this authorD. Treutter, Corresponding Author D. Treutter Department für Pflanzenwissenschaften, Fachgebiet Obstbau, Technische Universität München-Weihenstephan, Alte Akademie 16, 85350 Freising, Germany FG Obstbau Technische Universität München Alte Akademie 16 85350 Freising Germany E-mail: [email protected]Search for more papers by this author T. Strissel, T. Strissel Department für Pflanzenwissenschaften, Fachgebiet Obstbau, Technische Universität München-Weihenstephan, Alte Akademie 16, 85350 Freising, Germany Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, Technische Universität Wien, Getreidemarkt 9, 1060 Wien, AustriaSearch for more papers by this authorH. Halbwirth, H. Halbwirth Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, Technische Universität Wien, Getreidemarkt 9, 1060 Wien, AustriaSearch for more papers by this authorU. Hoyer, U. Hoyer Department für Pflanzenwissenschaften, Fachgebiet Obstbau, Technische Universität München-Weihenstephan, Alte Akademie 16, 85350 Freising, Germany Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, Technische Universität Wien, Getreidemarkt 9, 1060 Wien, AustriaSearch for more papers by this authorC. Zistler, C. Zistler Department für Pflanzenwissenschaften, Fachgebiet Obstbau, Technische Universität München-Weihenstephan, Alte Akademie 16, 85350 Freising, Germany Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, Technische Universität Wien, Getreidemarkt 9, 1060 Wien, AustriaSearch for more papers by this authorK. Stich, K. Stich Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, Technische Universität Wien, Getreidemarkt 9, 1060 Wien, AustriaSearch for more papers by this authorD. Treutter, Corresponding Author D. Treutter Department für Pflanzenwissenschaften, Fachgebiet Obstbau, Technische Universität München-Weihenstephan, Alte Akademie 16, 85350 Freising, Germany FG Obstbau Technische Universität München Alte Akademie 16 85350 Freising Germany E-mail: [email protected]Search for more papers by this author First published: 14 February 2008 https://doi.org/10.1055/s-2005-872989Citations: 46Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Abstract: Enhanced shoot growth and a decrease in flavonoid concentration in apple trees grown under high nitrogen (N) supply was observed in previous studies, along with increasing scab susceptibility of cultivar “Golden Delicious” after high N nutrition. Several hypotheses have suggested that there is a trade-off between primary and secondary metabolism because of competition for common substrates, but nothing is known about regulation at the enzyme level. In this study, a set of experiments was performed to elucidate the effect of N nutrition on the activities of key enzymes involved in flavonoid biosynthesis (phenylalanine ammonia-lyase [PAL], chalcone synthase/chalcone isomerase [CHS/CHI}, flavanone 3-hydroxylase [FHT], flavonol synthase [FLS], dihydroflavonol 4-reductase [DFR]) and the accumulation of different groups of phenylpropanoids. The inhibition of flavonoid accumulation by high N nutrition could be confirmed, but the influence of N supply on the flavonoid enzymes CHS/CHI, FHT, DFR, and FLS was not evident. However, PAL activity seems to be downregulated, thus forming a bottleneck resulting in a generally decreased flavonoid accumulation. Furthermore, the response of the scab-resistant cultivar “Rewena” to high N nutrition was not as strong as that of the susceptible cultivar “Golden Delicious”. References 1 Balsberg Pahlsson A.-M. Influence of nitrogen fertilization on minerals, carbohydrates, amino acids and phenolic compounds in beech (Fagus sylvatica L.) leaves. Tree Physiology. (1992) 10 93–100. 2 Bongue-Bartelsman M., Phillips D. A. Nitrogen stress regulates gene expression of enzymes in the flavonoid biosynthetic pathway of tomato. Plant Physiology and Biochemistry. (1995) 33 539–546. 3 da Cunha A. The estimation of L-phenylalanine ammonia-lyase shows phenylpropanoid biosynthesis to be regulated by L-phenylalanine supply and availability. Phytochemistry. (1987) 26 2723–2727. 4 Hakulinen J. R. Nitrogen-induced reduction in leaf phenolic level is not accomanied by increased rust frequency in a compatible willow (Salix myrsinifolia)-Melampsora rust interaction. Physiologia Plantarum. (1998) 102 101–110. 5 Hakulinen J. R., Julkunen-Tiitto R., Tahvanainen J. Does nitrogen fertilization have an impact on the trade-off between willow growth and defensive secondary metabolism?. Trees. (1995) 9 235–240. 6 Halbwirth H., Kampan W., Stich K., Fischer T. C., Meisel B., Forkmann G., Rademacher W. Biochemical and molecular biological investigations with respect to induction of fire blight resistance in apple and pear by transiently altering the flavonoid metabolism with specific enzyme inhibitors. Acta Hortitculturae. (2002) 590 485–492. 7 Herms D. A., Mattson W. J. The dilemma of plants: to grow and defend. The Quarterly Review of Biology. (1992) 67 283–335. 8 Jones C. G., Hartley S. E. A protein competition model of phenolic allocation. OIKOS. (1999) 86 27–44. 9 Kainulainen P., Holopainen J. K., Holopainen T. Combined effects of ozone and nitrogen on secondary compounds, amino acids, and aphid performance in Scots pine. Journal of Environmental Quality. (2000) 29 334–342. 10 Keinänen M., Julkunen-Tiitto R. High-performance liquid chromatographic determination of flavonoids in Betula pendula and Betula pubescens leaves. Journal of Chromatography A. (1998) 793 370–377. 11 Keski-Saari S., Julkunen-Tiitto R. Resource allocation in different parts of juvenile mountain birch plants: effect of nitrogen supply on seedling phenolics and growth. Physiologia Plantarum. (2003) 118 114–126. 12 Lavola A., Aphalo P. J., Lahti M., Julkunen-Tiitto R. Nutrient availability and the effect of increasing UV–B radiation on secondary plant compounds in Scots pine. Environmental and Experimental Botany. (2003) 49 49–60. 13 Lavola A., Julkunen-Tiitto R. The effect of elevated carbon dioxide and fertilization on the primary and secondary metabolites in birch, Betula pendula (Roth). Oecologia. (1994) 99 315–321. 14 Leser C., Treutter D. Effects of nitrogen supply on growth, contents of phenolic compounds and pathogen (scab) resistance of apple trees. Physiologia Plantarum. (2005) 123 49–56. 15 Loomis W. E. Growth-differentiation balance vs. carbonhydrate-nitrogen ratio. Proceedings of the American Society for Horticultural Science. (1932) 29 240–245. 16 Manibhushanrao K., Manian S. Changes in the phenol metabolism of rice cultivars with reference to nitrogen fertilization and sheath blight disease. Acta Phytopathologica et Entomologica Hungarica. (1995) 30 191–203. 17 Margna U. Control at the level of substrate supply - an alternative in the regulation of phenylpropanoid accumulation in plant cells. Phytochemistry. (1977) 16 419–426. 18 Matyssek R., Schnyder H., Elstner E. F., Munch J. C., Pretzsch H., Sandermann H. Growth and parasite defence in plants; the balance between resource sequestration and retention: in lieu of a guest editorial. Plant Biology. (2002) 4 133–136. 19 Mayr U., Treutter D., Santos-Buelga C., Bauer H., Feucht W. Developmental changes in the phenolic concentrations of “Golden Delicious” apple fruits and leaves. Phytochemistry. (1995) 38 1151–1155. 20 Mayr U., Michalek S., Treutter D., Feucht W. Phenolic compounds of apple and their relationship to scab resistance. Journal of Phytopathology. (1997) 145 69–75. 21 Muzika R. M., Pregitzer K. S. Effect of nitrogen fertilization on leaf phenolic production of grand fir seedlings. Trees. (1992) 6 241–244. 22 Picinelli A., Dapena E., Mangas J. J. Polyphenolic pattern in apple tree leaves in relation to scab resistance. A preliminary study. Journal of Agricultural and Food Chemistry. (1997) 43 2273–2278. 23 Roemmelt S., Zimmermann N., Rademacher W., Treutter D. Formation of novel flavonoids in apple (Malus × domestica) treated with the 2-oxoglutarate-dependent dioxygenase inhibitor prohexadione-Ca. Phytochemistry. (2003) 64 709–716. 24 Rühmann S., Leser C., Bannert M., Treutter D. Relationship between growth, secondary metabolism, and resistance of apple. Plant Biology. (2002) 4 137–143. 25 Rühmann S., Treutter D. Effect of N-nutrition in apple on the response of its secondary metabolism to prohexadione-Ca treatment. European Journal of Horticultural Science. (2003) 68 152–159. 26 Sandermann H., Strominger L. Purification and properties of C55-isoprenoid alcohol phosphokinase from Staphylococcus aureus. Journal of Biological Chemistry. (1972) 247 5123–5131. 27 Stewart A. J., Chapman W., Jenkins G. I., Graham I., Martin T., Crozier A. The effect of nitrogen and phosphorus deficiency on flavonol accumulation in plant tissues. Plant, Cell and Environment. (2001) 24 1189–1197. 28 Tan S. C. Phenylalanine ammonia-lyase and the phenylalanine ammonia-lyase inactivating system: effects of light, temperature and mineral deficiencies. Australian Journal of Plant Physiology. (1980) 7 159–167. 29 Treutter D. Chemical reaction detection of catechins and proanthocyanidins with 4-dimethylaminocinnamaldehyde. Journal of Chromatography. (1989) 467 185–193. 30 Treutter D., Santos-Buelga C., Gutmann M., Kolodziej H. Identification of flavan-3-ols and procyanidins by HPLC and chemical reaction detection. Journal of Chromatography A. (1994) 667 290–297. Citing Literature Volume7, Issue6November 2005Pages 677-685 ReferencesRelatedInformation
Publication Year: 2005
Publication Date: 2005-11-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 60
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