Title: Singlet oxygen kinetics inside living cells: observation of endogenous quencher consumption and consequences for the spatial resolution in time-resolved measurements
Abstract: The consumption of singlet oxygen quenchers during illumination of cells incubated with photosensitizers is a serious issue for the determination of singlet oxygen kinetics in cells. Using LNCaP cells incubated with pheophorbide a it will be shown, that already an illumination of 100 nJ/cell dramatically changes the value of the observables. This finding has consequences for the spatial resolution of any time-resolved singlet oxygen measurement, since the radiative rate constant of singlet oxygen in aqueous solutions is very small.
Publication Year: 2009
Publication Date: 2009-06-29
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 8
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