Title: An autoradiographic study of the macronuclear changes occurring in Paramecium aurelia during autogamy
Abstract: The fate of the macronucleus of Paramecium aurelia during and after autogamy has been studied in organisms grown in an axenic medium containing tritiated thymidine. Autoradiographs indicate that the DNA of the old macronucleus is not preferentially reutilized for the formation of DNA in the new macronucleus. Growth of cells in a medium containing 25 μc of tritium per ml showed marked labeling of the cytoplasm. Hydrolysis with hot acid according to Feulgen did not remove the label. This may indicate the presence of DNA in the cytoplasm. Chromatography on paper of the cellular fractions revealed that uridylic acid from RNA is radioactive. Paramecium aurelia appears capable of converting thymidine to RNA precursors followed by incorporation into RNA.
Publication Year: 1962
Publication Date: 1962-03-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 21
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