Title: Neurotransmitters adenosine triphosphate and noradrenaline induce nitric oxide release in rat vas deferens
Abstract: 1 In rat vas deferens, electrical field stimulation (EFS) evoked a muscular biphasic tetrodotoxin (TTX)‐sensitive contractile response. 2 The amplitude of this response increased with the frequency of stimulation. 3 After each stimulation, nitric oxide (NO) release was assayed and found to be released in a frequency‐dependent manner. 4 NO release also occurred after treatment with exogenous neurotransmitters, adenosine 5′‐triphosphate (ATP) and noradrenaline (NA). 5 Prazosin and pyridoxalphosphate‐6‐azophenyl‐2′,4′‐disulphonic acid (PPADS), respective antagonists of α1‐adrenoceptors and P2x purinoceptors, inhibited NO release induced by NA and ATP. Both prazosin and PPADS inhibited NO release by EFS. 6 TTX failed to modify the NO release induced by exogenous neurotransmitters but abolished the release of NO and contractile response by EFS. 7 EFS and released noradrenaline and ATP induce the release of NO through postjunctional α1‐adrenoceptors and P2x‐purinoceptors, respectively.
Publication Year: 2000
Publication Date: 2000-06-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 4
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot