Title: FTIR Vibrational Circular Dichroism Of Oligopeptides Related To Polyproline
Abstract:Vibrational Circular Dichroism (VCD) data can be routinely measured by FTIR. On our FTIR VCD instrument it is possible to obtain a spectrum where baseline correction is accomplished with solvent only....Vibrational Circular Dichroism (VCD) data can be routinely measured by FTIR. On our FTIR VCD instrument it is possible to obtain a spectrum where baseline correction is accomplished with solvent only. Such capability is important for measuring biological systems. Several polypeptides that have been assigned to be in 'random coil' conformation all give an amide I VCD pattern which has the same sign and bandshape as poly-L-proline II but a smaller magnitude. This is consistent with a previous proposal by Tiffany & Krimm that the 'random coil' conformation of charged polypeptides actually has a significant local ordering in the form of a left-handed extended helix. To investigate this problem further we have studied the effects of temperature and chain length on the VCD bandshape of this conformational type. The latter studies were done with (Pro)n, n=2-7. Our results indicate that even at the level of (Pro)4 the VCD spectrum has the same bandshape, sign and intensity as found in the 'random coil' poly-L-glutamic acid spectrum. Data on various 'random coil' systems will be compared to the model studies we have done.Read More
Publication Year: 1989
Publication Date: 1989-12-01
Language: en
Type: article
Indexed In: ['crossref']
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