Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2090889271', 'doi': 'https://doi.org/10.1002/bit.22452', 'title': 'Using microarray technology to select housekeeping genes in Chinese hamster ovary cells', 'display_name': 'Using microarray technology to select housekeeping genes in Chinese hamster ovary cells', 'publication_year': 2009, 'publication_date': '2009-06-25', 'ids': {'openalex': 'https://openalex.org/W2090889271', 'doi': 'https://doi.org/10.1002/bit.22452', 'mag': '2090889271', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/19557832'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1002/bit.22452', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S151564723', 'display_name': 'Biotechnology and Bioengineering', 'issn_l': '0006-3592', 'issn': ['0006-3592', '1097-0290'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5104109589', 'display_name': 'Scott M. Bahr', 'orcid': None}, 'institutions': [], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Scott M. Bahr', 'raw_affiliation_strings': ['Cell Sciences and Development, SAFC Biosciences, 2909 Laclede Avenue, St. Louis, Missouri 63103', 'fax: (314)286-7645', 'telephone: (314)771-5765'], 'affiliations': [{'raw_affiliation_string': 'Cell Sciences and Development, SAFC Biosciences, 2909 Laclede Avenue, St. Louis, Missouri 63103', 'institution_ids': []}, {'raw_affiliation_string': 'telephone: (314)771-5765', 'institution_ids': []}, {'raw_affiliation_string': 'fax: (314)286-7645', 'institution_ids': []}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5011019803', 'display_name': 'Trissa Borgschulte', 'orcid': None}, 'institutions': [], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Trissa Borgschulte', 'raw_affiliation_strings': ['Cell Sciences and Development, SAFC Biosciences, 2909 Laclede Avenue, St. Louis, Missouri 63103', 'fax: (314)286-7645', 'telephone: (314)771-5765'], 'affiliations': [{'raw_affiliation_string': 'Cell Sciences and Development, SAFC Biosciences, 2909 Laclede Avenue, St. Louis, Missouri 63103', 'institution_ids': []}, {'raw_affiliation_string': 'telephone: (314)771-5765', 'institution_ids': []}, {'raw_affiliation_string': 'fax: (314)286-7645', 'institution_ids': []}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5026146066', 'display_name': 'Kevin J. Kayser', 'orcid': None}, 'institutions': [], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Kevin J. Kayser', 'raw_affiliation_strings': ['Cell Sciences and Development, SAFC Biosciences, 2909 Laclede Avenue, St. Louis, Missouri 63103', 'fax: (314)286-7645', 'telephone: (314)771-5765'], 'affiliations': [{'raw_affiliation_string': 'telephone: (314)771-5765', 'institution_ids': []}, {'raw_affiliation_string': 'fax: (314)286-7645', 'institution_ids': []}, {'raw_affiliation_string': 'Cell Sciences and Development, SAFC Biosciences, 2909 Laclede Avenue, St. Louis, Missouri 63103', 'institution_ids': []}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5056567265', 'display_name': 'Nan Lin', 'orcid': 'https://orcid.org/0000-0002-1878-6465'}, 'institutions': [], 'countries': ['US'], 'is_corresponding': True, 'raw_author_name': 'Nan Lin', 'raw_affiliation_strings': ['Cell Sciences and Development, SAFC Biosciences, 2909 Laclede Avenue, St. Louis, Missouri 63103', 'fax: (314)286-7645', 'telephone: (314)771-5765'], 'affiliations': [{'raw_affiliation_string': 'Cell Sciences and Development, SAFC Biosciences, 2909 Laclede Avenue, St. Louis, Missouri 63103', 'institution_ids': []}, {'raw_affiliation_string': 'telephone: (314)771-5765', 'institution_ids': []}, {'raw_affiliation_string': 'fax: (314)286-7645', 'institution_ids': []}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 0, 'corresponding_author_ids': ['https://openalex.org/A5056567265'], 'corresponding_institution_ids': [], 'apc_list': {'value': 4840, 'currency': 'USD', 'value_usd': 4840, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 1.196, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 38, 'citation_normalized_percentile': {'value': 0.86538, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 92, 'max': 93}, 'biblio': {'volume': '104', 'issue': '5', 'first_page': '1041', 'last_page': '1046'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11663', 'display_name': 'Viral Infectious Diseases and Gene Expression in Insects', 'score': 0.9998, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11663', 'display_name': 'Viral Infectious Diseases and Gene Expression in Insects', 'score': 0.9998, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11970', 'display_name': 'Molecular Biology Techniques and Applications', 'score': 0.9993, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10613', 'display_name': 'Virus-based gene therapy research', 'score': 0.9923, 'subfield': {'id': 'https://openalex.org/subfields/1311', 'display_name': 'Genetics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/housekeeping-gene', 'display_name': 'Housekeeping gene', 'score': 0.8945416}, {'id': 'https://openalex.org/keywords/gene-chip-analysis', 'display_name': 'Gene chip analysis', 'score': 0.5092982}], 'concepts': [{'id': 'https://openalex.org/C33922130', 'wikidata': 'https://www.wikidata.org/wiki/Q419232', 'display_name': 'Housekeeping gene', 'level': 4, 'score': 0.8945416}, {'id': 'https://openalex.org/C175656101', 'wikidata': 'https://www.wikidata.org/wiki/Q56646', 'display_name': 'Chinese hamster ovary cell', 'level': 3, 'score': 0.8342819}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.6635961}, {'id': 'https://openalex.org/C186836561', 'wikidata': 'https://www.wikidata.org/wiki/Q1931516', 'display_name': 'Microarray', 'level': 4, 'score': 0.63430953}, {'id': 'https://openalex.org/C95371953', 'wikidata': 'https://www.wikidata.org/wiki/Q591745', 'display_name': 'DNA microarray', 'level': 4, 'score': 0.5870503}, {'id': 'https://openalex.org/C18431079', 'wikidata': 'https://www.wikidata.org/wiki/Q1502169', 'display_name': 'Gene expression profiling', 'level': 4, 'score': 0.5748027}, {'id': 'https://openalex.org/C8415881', 'wikidata': 'https://www.wikidata.org/wiki/Q6839217', 'display_name': 'Microarray analysis techniques', 'level': 4, 'score': 0.5233316}, {'id': 'https://openalex.org/C150194340', 'wikidata': 'https://www.wikidata.org/wiki/Q26972', 'display_name': 'Gene expression', 'level': 3, 'score': 0.5212146}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.5201107}, {'id': 'https://openalex.org/C24361400', 'wikidata': 'https://www.wikidata.org/wiki/Q6839217', 'display_name': 'Gene chip analysis', 'level': 5, 'score': 0.5092982}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.446494}, {'id': 'https://openalex.org/C70721500', 'wikidata': 'https://www.wikidata.org/wiki/Q177005', 'display_name': 'Computational biology', 'level': 1, 'score': 0.44359455}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.4126612}, {'id': 'https://openalex.org/C81885089', 'wikidata': 'https://www.wikidata.org/wiki/Q189082', 'display_name': 'Cell culture', 'level': 2, 'score': 0.23889908}], 'mesh': [{'descriptor_ui': 'D016466', 'descriptor_name': 'CHO Cells', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D020869', 'descriptor_name': 'Gene Expression Profiling', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D020411', 'descriptor_name': 'Oligonucleotide Array Sequence Analysis', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D000818', 'descriptor_name': 'Animals', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006224', 'descriptor_name': 'Cricetinae', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003412', 'descriptor_name': 'Cricetulus', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D020133', 'descriptor_name': 'Reverse Transcriptase Polymerase Chain Reaction', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013312', 'descriptor_name': 'Stress, Physiological', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1002/bit.22452', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S151564723', 'display_name': 'Biotechnology and Bioengineering', 'issn_l': '0006-3592', 'issn': ['0006-3592', '1097-0290'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/19557832', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [{'score': 0.55, 'display_name': 'Life on land', 'id': 'https://metadata.un.org/sdg/15'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 32, 'referenced_works': ['https://openalex.org/W1571283687', 'https://openalex.org/W1577577364', 'https://openalex.org/W1968847190', 'https://openalex.org/W1974505324', 'https://openalex.org/W1977742604', 'https://openalex.org/W1982264399', 'https://openalex.org/W1983133093', 'https://openalex.org/W1995709832', 'https://openalex.org/W2001582636', 'https://openalex.org/W2025492442', 'https://openalex.org/W2044509030', 'https://openalex.org/W2057464020', 'https://openalex.org/W2059251538', 'https://openalex.org/W2080660790', 'https://openalex.org/W2093682191', 'https://openalex.org/W2094554391', 'https://openalex.org/W2099050424', 'https://openalex.org/W2099824652', 'https://openalex.org/W2102102358', 'https://openalex.org/W2104455852', 'https://openalex.org/W2105423509', 'https://openalex.org/W2116710527', 'https://openalex.org/W2118540273', 'https://openalex.org/W2123793943', 'https://openalex.org/W2126855363', 'https://openalex.org/W2128443399', 'https://openalex.org/W2135598172', 'https://openalex.org/W2143146123', 'https://openalex.org/W2144271845', 'https://openalex.org/W2148927308', 'https://openalex.org/W2149145325', 'https://openalex.org/W2154127252'], 'related_works': ['https://openalex.org/W4310366902', 'https://openalex.org/W3023720143', 'https://openalex.org/W2490137852', 'https://openalex.org/W2412120523', 'https://openalex.org/W2160130936', 'https://openalex.org/W2061675700', 'https://openalex.org/W2048038364', 'https://openalex.org/W1971329594', 'https://openalex.org/W170658859', 'https://openalex.org/W1536722219'], 'abstract_inverted_index': {'In': [0], 'the': [1, 85, 97], 'present': [2], 'study,': [3], 'we': [4], 'have': [5], 'identified': [6], 'species-specific': [7], 'housekeeping': [8], 'genes': [9, 100], '(HKGs)': [10], 'for': [11, 26, 139], 'Chinese': [12], 'Hamster': [13], 'Ovary': [14], '(CHO)': [15], 'cells': [16], 'using': [17, 54, 115], 'data': [18], 'from': [19, 66, 84], 'microarray': [20, 59, 86], 'gene': [21, 140], 'expression': [22, 34, 64, 83, 141, 148], 'profiling.': [23], 'HKGs': [24, 76, 92, 121], 'suitable': [25], 'quantitative': [27], 'RT-PCR': [28], 'normalization': [29], 'should': [30], 'display': [31], 'relatively': [32], 'stable': [33], 'levels': [35], 'across': [36], 'experimental': [37], 'conditions.': [38], 'We': [39, 69], 'analyzed': [40], 'transcription': [41], 'profiles': [42], 'of': [43, 74, 99, 119], 'several': [44], 'IgG-producing': [45], 'recombinant': [46], 'CHO': [47, 57, 144], 'cell': [48], 'lines': [49], 'under': [50], 'numerous': [51], 'culture': [52], 'conditions': [53], 'a': [55, 71, 136, 146], 'custom': [56], 'DNA': [58], 'platform': [60, 149], 'and': [61, 95, 129], 'calculated': [62], 'relative': [63], 'variability': [65, 81], '124': [67], 'microarrays.': [68], 'selected': [70], 'novel': [72], 'panel': [73, 103], 'candidate': [75, 110], 'based': [77], 'on': [78, 101], 'their': [79], 'low': [80], 'in': [82, 143, 151], 'data.': [87], 'Compared': [88], 'to': [89], 'three': [90], 'traditional': [91], '(Gapdh,': [93], 'Actb,': [94], 'B2m),': [96], 'majority': [98], 'this': [102], 'demonstrated': [104], 'lower': [105], 'or': [106], 'equal': [107], 'variability.': [108], 'Each': [109], 'HKG': [111], 'was': [112], 'then': [113], 'validated': [114], 'qRT-PCR.': [116], 'Final': [117], 'selection': [118], 'CHO-specific': [120], 'include': [122], 'Actr5,': [123], 'Eif3i,': [124], 'Hirip3,': [125], 'Pabpn1,': [126], 'Vezt,': [127], 'Cog1,': [128], 'Yaf2.': [130], 'The': [131], 'results': [132], 'reported': [133], 'here': [134], 'provide': [135], 'useful': [137], 'tool': [138], 'analyses': [142], 'cells,': [145], 'critical': [147], 'used': [150], 'biotherapeutics.': [152]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2090889271', 'counts_by_year': [{'year': 2023, 'cited_by_count': 3}, {'year': 2022, 'cited_by_count': 1}, {'year': 2021, 'cited_by_count': 2}, {'year': 2020, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 5}, {'year': 2018, 'cited_by_count': 1}, {'year': 2017, 'cited_by_count': 2}, {'year': 2016, 'cited_by_count': 3}, {'year': 2015, 'cited_by_count': 5}, {'year': 2014, 'cited_by_count': 1}, {'year': 2013, 'cited_by_count': 5}, {'year': 2012, 'cited_by_count': 1}], 'updated_date': '2025-01-05T15:21:04.690610', 'created_date': '2016-06-24'}