Title: Widespread expression of gephyrin, a putative glycine receptor-tubulin linker protein, in rat brain
Abstract: The peripheral membrane protein gephyrin co-purifies with the inhibitory postsynaptic glycine receptor (GlyR) of mammalian spinal cord. By immunoelectron microscopy, gephyrin has been localized at the cytoplasmic face of glycinergic postsynaptic membrane specializations. Here, we used specific monoclonal antibodies to demonstrate the presence of gephyrin in all regions of rat brain known to contain synapses and compared its histochemical distribution with that of GlyR antigens. In most brain structures, gephyrin is expressed independently of the GlyR α1 subunit, but its distribution is very similar to the pattern obtained with mAb 4a, a monoclonal antibody recognizing the known GlyR α and β subunits. Our data suggest a much wider distribution of gephyrin and GlyR proteins in the mammalian CNS than anticipated previously.
Publication Year: 1993
Publication Date: 1993-09-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 147
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