Publication Information

{'id': 'https://openalex.org/W2090509819', 'doi': 'https://doi.org/10.1002/jmr.300030509', 'title': 'Characterization of specific interactions of coenzymes, regulatory nucleotides and cibacron blue with nucleotide binding domains of enzymes by analytical affinity chromatography', 'display_name': 'Characterization of specific interactions of coenzymes, regulatory nucleotides and cibacron blue with nucleotide binding domains of enzymes by analytical affinity chromatography', 'publication_year': 1990, 'publication_date': '1990-10-01', 'ids': {'openalex': 'https://openalex.org/W2090509819', 'doi': 'https://doi.org/10.1002/jmr.300030509', 'mag': '2090509819', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/2096889'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1002/jmr.300030509', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S205370667', 'display_name': 'Journal of Molecular Recognition', 'issn_l': '0952-3499', 'issn': ['0952-3499', '1099-1352'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5032290933', 'display_name': 'Wayne C. Thresher', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I137902535', 'display_name': 'North Carolina State University', 'ror': 'https://ror.org/04tj63d06', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I137902535']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Wayne C. Thresher', 'raw_affiliation_strings': ['Department of Food Science and Biochemistry, North Carolina State University, Raleigh 27695-7624.'], 'affiliations': [{'raw_affiliation_string': 'Department of Food Science and Biochemistry, North Carolina State University, Raleigh 27695-7624.', 'institution_ids': ['https://openalex.org/I137902535']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5044060660', 'display_name': 'Harold E. Swaisgood', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I137902535', 'display_name': 'North Carolina State University', 'ror': 'https://ror.org/04tj63d06', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I137902535']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Harold E. Swaisgood', 'raw_affiliation_strings': ['Department of Food Science and Biochemistry, North Carolina State University, Raleigh, NC 27695–7624, USA'], 'affiliations': [{'raw_affiliation_string': 'Department of Food Science and Biochemistry, North Carolina State University, Raleigh, NC 27695–7624, USA', 'institution_ids': ['https://openalex.org/I137902535']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 3450, 'currency': 'USD', 'value_usd': 3450, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 0.612, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 13, 'citation_normalized_percentile': {'value': 0.618943, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 81, 'max': 82}, 'biblio': {'volume': '3', 'issue': '5-6', 'first_page': '220', 'last_page': '228'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T12770', 'display_name': 'Amino Acid Enzymes and Metabolism', 'score': 0.9998, 'subfield': {'id': 'https://openalex.org/subfields/1303', 'display_name': 'Biochemistry'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T12770', 'display_name': 'Amino Acid Enzymes and Metabolism', 'score': 0.9998, 'subfield': {'id': 'https://openalex.org/subfields/1303', 'display_name': 'Biochemistry'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10532', 'display_name': 'Molecular Sensors and Ion Detection', 'score': 0.9991, 'subfield': {'id': 'https://openalex.org/subfields/1607', 'display_name': 'Spectroscopy'}, 'field': {'id': 'https://openalex.org/fields/16', 'display_name': 'Chemistry'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T10155', 'display_name': 'Nitric Oxide and Endothelin Effects', 'score': 0.9983, 'subfield': {'id': 'https://openalex.org/subfields/2737', 'display_name': 'Physiology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/ternary-complex', 'display_name': 'Ternary complex', 'score': 0.7632452}, {'id': 'https://openalex.org/keywords/dissociation-constant', 'display_name': 'Dissociation constant', 'score': 0.7512855}], 'concepts': [{'id': 'https://openalex.org/C86756495', 'wikidata': 'https://www.wikidata.org/wiki/Q7702931', 'display_name': 'Ternary complex', 'level': 3, 'score': 0.7632452}, {'id': 'https://openalex.org/C74998103', 'wikidata': 'https://www.wikidata.org/wiki/Q898254', 'display_name': 'Dissociation constant', 'level': 3, 'score': 0.7512855}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.738224}, {'id': 'https://openalex.org/C179247698', 'wikidata': 'https://www.wikidata.org/wiki/Q382799', 'display_name': 'Affinity chromatography', 'level': 3, 'score': 0.7098293}, {'id': 'https://openalex.org/C43617362', 'wikidata': 'https://www.wikidata.org/wiki/Q170050', 'display_name': 'Chromatography', 'level': 1, 'score': 0.58291817}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.5143136}, {'id': 'https://openalex.org/C197957613', 'wikidata': 'https://www.wikidata.org/wiki/Q407280', 'display_name': 'Cofactor', 'level': 3, 'score': 0.5009365}, {'id': 'https://openalex.org/C2776317432', 'wikidata': 'https://www.wikidata.org/wiki/Q412547', 'display_name': 'Dehydrogenase', 'level': 3, 'score': 0.49676925}, {'id': 'https://openalex.org/C2777318727', 'wikidata': 'https://www.wikidata.org/wiki/Q409464', 'display_name': 'Lactate dehydrogenase', 'level': 3, 'score': 0.44425043}, {'id': 'https://openalex.org/C28212737', 'wikidata': 'https://www.wikidata.org/wiki/Q5381543', 'display_name': 'Enzyme assay', 'level': 3, 'score': 0.41234803}, {'id': 'https://openalex.org/C33633552', 'wikidata': 'https://www.wikidata.org/wiki/Q904145', 'display_name': 'Immobilized enzyme', 'level': 3, 'score': 0.41184205}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.31938753}, {'id': 'https://openalex.org/C170493617', 'wikidata': 'https://www.wikidata.org/wiki/Q208467', 'display_name': 'Receptor', 'level': 2, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D002846', 'descriptor_name': 'Chromatography, Affinity', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D003067', 'descriptor_name': 'Coenzymes', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D004396', 'descriptor_name': 'Coloring Agents', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D005969', 'descriptor_name': 'Glutamate Dehydrogenase', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D007770', 'descriptor_name': 'L-Lactate Dehydrogenase', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D009711', 'descriptor_name': 'Nucleotides', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D014227', 'descriptor_name': 'Triazines', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D000818', 'descriptor_name': 'Animals', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001665', 'descriptor_name': 'Binding Sites', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002417', 'descriptor_name': 'Cattle', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002846', 'descriptor_name': 'Chromatography, Affinity', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003067', 'descriptor_name': 'Coenzymes', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004396', 'descriptor_name': 'Coloring Agents', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004800', 'descriptor_name': 'Enzymes, Immobilized', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D004800', 'descriptor_name': 'Enzymes, Immobilized', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005069', 'descriptor_name': 'Evaluation Studies as Topic', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005898', 'descriptor_name': 'Glass', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005969', 'descriptor_name': 'Glutamate Dehydrogenase', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007700', 'descriptor_name': 'Kinetics', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007770', 'descriptor_name': 'L-Lactate Dehydrogenase', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009243', 'descriptor_name': 'NAD', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D009243', 'descriptor_name': 'NAD', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009711', 'descriptor_name': 'Nucleotides', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011817', 'descriptor_name': 'Rabbits', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013386', 'descriptor_name': 'Succinates', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013884', 'descriptor_name': 'Thiosulfate Sulfurtransferase', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013884', 'descriptor_name': 'Thiosulfate Sulfurtransferase', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D014227', 'descriptor_name': 'Triazines', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1002/jmr.300030509', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S205370667', 'display_name': 'Journal of Molecular Recognition', 'issn_l': '0952-3499', 'issn': ['0952-3499', '1099-1352'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/2096889', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [{'score': 0.59, 'display_name': 'Clean water and sanitation', 'id': 'https://metadata.un.org/sdg/6'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 25, 'referenced_works': ['https://openalex.org/W120446455', 'https://openalex.org/W1497638178', 'https://openalex.org/W1564237121', 'https://openalex.org/W1570636691', 'https://openalex.org/W1581740874', 'https://openalex.org/W1967328861', 'https://openalex.org/W1969005409', 'https://openalex.org/W1975630793', 'https://openalex.org/W1991461113', 'https://openalex.org/W2031369081', 'https://openalex.org/W2047121149', 'https://openalex.org/W2052327543', 'https://openalex.org/W2060281851', 'https://openalex.org/W2070327253', 'https://openalex.org/W2081989734', 'https://openalex.org/W2082101256', 'https://openalex.org/W2083478206', 'https://openalex.org/W2125625153', 'https://openalex.org/W2156076210', 'https://openalex.org/W2214447253', 'https://openalex.org/W2321129318', 'https://openalex.org/W2413445386', 'https://openalex.org/W630729105', 'https://openalex.org/W79111353', 'https://openalex.org/W936738925'], 'related_works': ['https://openalex.org/W2411367265', 'https://openalex.org/W2328197679', 'https://openalex.org/W2091247790', 'https://openalex.org/W2090509819', 'https://openalex.org/W2061718200', 'https://openalex.org/W2060044165', 'https://openalex.org/W2012601162', 'https://openalex.org/W2000394801', 'https://openalex.org/W1989062181', 'https://openalex.org/W1523595220'], 'abstract_inverted_index': {'The': [0, 84, 218], 'dissociation': [1, 131, 141], 'constant': [2, 132], 'for': [3, 159, 220], 'the': [4, 38, 44, 50, 62, 107, 115, 124, 130, 134, 140, 160, 175, 208, 212, 226, 234, 245, 252], 'complex': [5, 60, 86, 162, 228, 236], 'of': [6, 53, 57, 61, 74, 118, 129, 133, 147, 170, 178, 185, 207, 215, 244], 'rhodanese': [7, 17], 'and': [8, 28, 64, 90, 102, 156, 182, 195, 259], 'Cibacron': [9, 78, 104], 'Blue,': [10, 30], 'determined': [11, 143, 158], 'by': [12, 71, 94, 144, 248], 'analytical': [13, 95], 'affinity': [14, 96, 219, 253], 'chromatography': [15, 73, 97, 122, 146], 'using': [16, 98, 123, 200], 'immobilized': [18, 103, 125, 150, 201], 'on': [19, 76, 149], 'controlled-pore': [20], 'glass': [21], '(CPG)': [22], 'beads': [23], '(200': [24], 'nm': [25], 'pore': [26], 'diameter)': [27], 'aminohexyl-Cibacron': [29], 'was': [31, 68, 92, 197], '44': [32], 'microM': [33, 238, 258], 'which': [34], 'agreed': [35], 'well': [36], 'with': [37, 110, 191], 'kinetic': [39], 'inhibition': [40], 'constant,': [41], 'suggesting': [42], 'that': [43, 157, 168], 'dye': [45, 63, 108, 126], 'binds': [46], 'at': [47], 'or': [48], 'near': [49], 'active': [51, 116], 'site': [52, 117, 247], 'this': [54], 'enzyme.': [55], 'Formation': [56, 177], 'a': [58], 'binary': [59, 85, 235], 'lactate': [65], 'dehydrogenase': [66, 189], '(LDH)': [67], 'also': [69, 198], 'characterized': [70, 93], 'direct': [72, 145], 'LDH': [75, 89, 101, 151, 171], 'CPG/immobilized': [77, 100], 'Blue': [79], '(KD': [80, 152, 163], '=': [81, 153, 164], '0.29': [82], 'microM).': [83], 'formed': [87], 'between': [88, 139], 'NADH': [91, 111, 148], 'both': [99, 255], 'Blue.': [105], 'Since': [106], 'competes': [109], 'in': [112, 225, 254], 'binding': [113], 'to': [114, 233], 'LDH,': [119], 'competitive': [120], 'elution': [121], 'allows': [127, 205], 'determination': [128], 'soluble': [135, 161], 'LDH.NADH': [136], 'complex.': [137], 'Agreement': [138], 'constants': [142], '1.4': [154], 'microM)': [155, 166], '2.4': [165], 'indicates': [167], 'immobilization': [169], 'did': [172], 'not': [173], 'affect': [174], 'interaction.': [176], 'various': [179], 'binary,': [180], 'ternary': [181, 227], 'quaternary': [183], 'complexes': [184, 256], 'bovine': [186], 'liver': [187], 'glutamate': [188], '(GDH)': [190], 'glutamate,': [192], 'NADPH,': [193], 'NADH,': [194], 'ADP': [196, 249], 'investigated': [199], 'GDH.': [202], 'This': [203], 'approach': [204], 'characterization': [206], 'enzyme/ligand': [209], 'interactions': [210], 'without': [211], 'complicating': [213], 'effect': [214], 'enzyme': [216], 'self-association.': [217], 'NADPH': [221], 'is': [222], 'considerably': [223], 'greater': [224], '(including': [229], 'glutamate)': [230], 'as': [231], 'compared': [232], '(0.38': [237], 'vs': [239], '22': [240], 'microM);': [241], 'however,': [242], 'occupancy': [243], 'regulatory': [246], 'greatly': [250], 'reduces': [251], '(6.4': [257], '43': [260], 'microM,': [261], 'respectively).(ABSTRACT': [262], 'TRUNCATED': [263], 'AT': [264], '250': [265], 'WORDS)': [266]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2090509819', 'counts_by_year': [{'year': 2019, 'cited_by_count': 1}, {'year': 2013, 'cited_by_count': 1}], 'updated_date': '2024-12-10T13:47:05.181773', 'created_date': '2016-06-24'}