Title: 1102 Lack of prognostic significance of immunohistochemical detection of P53 in non-small cell lung cancer (NSCLC)
Abstract: The aim of this study was to establish whether immunohistochemical detected expression of p53 protein is related to prognosis in NSCLC. From 1984 to 1991 tissue samples were obtained from 186 surgical treated patients with NSCLC (squamous cell = SQ 104; adenocarcinoma = AD 59; large cell carcinoma = LA 22). The protein-product of the tumor suppressor gene p53 was analyzed on cryostat sections using the peroxidase Labelled Strept-Avidin-Biotin technique. P53 protein was visualized by the monoclonal antibody DO7 (DAKO), the percentage of tumor cells with nuclear staining (grade 0, grade I = 1–29%, grade 2 = 30–59%, grade 3 = 60–100%)was estimated and results were correlated with clinical characteristics. Grade 3 expression was fuund in 45% of tumors. p53 expression was neither correlated to tumor size, nodal status, stage or survival p53 may be important in the carcinogenesis of lung cancer but of little significance in established tumors. The aim of this study was to establish whether immunohistochemical detected expression of p53 protein is related to prognosis in NSCLC. From 1984 to 1991 tissue samples were obtained from 186 surgical treated patients with NSCLC (squamous cell = SQ 104; adenocarcinoma = AD 59; large cell carcinoma = LA 22). The protein-product of the tumor suppressor gene p53 was analyzed on cryostat sections using the peroxidase Labelled Strept-Avidin-Biotin technique. P53 protein was visualized by the monoclonal antibody DO7 (DAKO), the percentage of tumor cells with nuclear staining (grade 0, grade I = 1–29%, grade 2 = 30–59%, grade 3 = 60–100%)was estimated and results were correlated with clinical characteristics. Grade 3 expression was fuund in 45% of tumors. p53 expression was neither correlated to tumor size, nodal status, stage or survival p53 may be important in the carcinogenesis of lung cancer but of little significance in established tumors.
Publication Year: 1995
Publication Date: 1995-11-01
Language: en
Type: article
Indexed In: ['crossref']
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