A Highly Purified RNA Polymerase II Elongation Control System

Name: Work Video:
Duration: 3 min 30 s
Dan B. Renner; Yuki Yamaguchi; Tadashi Wada; Hiroshi Handa; David H. Price
{'id': 'https://openalex.org/W2086949060', 'doi': 'https://doi.org/10.1074/jbc.m104967200', 'title': 'A Highly Purified RNA Polymerase II Elongation Control System', 'display_name': 'A Highly Purified RNA Polymerase II Elongation Control System', 'publication_year': 2001, 'publication_date': '2001-11-01', 'ids': {'openalex': 'https://openalex.org/W2086949060', 'doi': 'https://doi.org/10.1074/jbc.m104967200', 'mag': '2086949060', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/11553615'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m104967200', 'pdf_url': 'http://www.jbc.org/article/S0021925819831277/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'hybrid', 'oa_url': 'http://www.jbc.org/article/S0021925819831277/pdf', 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5018460961', 'display_name': 'Dan B. Renner', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I126307644', 'display_name': 'University of Iowa', 'ror': 'https://ror.org/036jqmy94', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I126307644']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Dan B. Renner', 'raw_affiliation_strings': ['Department of Biochemistry, University of Iowa, Iowa City, Iowa, 52242'], 'affiliations': [{'raw_affiliation_string': 'Department of Biochemistry, University of Iowa, Iowa City, Iowa, 52242', 'institution_ids': ['https://openalex.org/I126307644']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5001576132', 'display_name': 'Yuki Yamaguchi', 'orcid': 'https://orcid.org/0000-0002-0197-614X'}, 'institutions': [{'id': 'https://openalex.org/I114531698', 'display_name': 'Tokyo Institute of Technology', 'ror': 'https://ror.org/0112mx960', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I114531698']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Yuki Yamaguchi', 'raw_affiliation_strings': ['Faculty of Bioscience and Biotechnology and Frontier Collaborative Research Center, Tokyo Institute of Technology, Yokohama, Japan 226-8501'], 'affiliations': [{'raw_affiliation_string': 'Faculty of Bioscience and Biotechnology and Frontier Collaborative Research Center, Tokyo Institute of Technology, Yokohama, Japan 226-8501', 'institution_ids': ['https://openalex.org/I114531698']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5105889207', 'display_name': 'Tadashi Wada', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I114531698', 'display_name': 'Tokyo Institute of Technology', 'ror': 'https://ror.org/0112mx960', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I114531698']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Tadashi Wada', 'raw_affiliation_strings': ['Faculty of Bioscience and Biotechnology and Frontier Collaborative Research Center, Tokyo Institute of Technology, Yokohama, Japan 226-8501'], 'affiliations': [{'raw_affiliation_string': 'Faculty of Bioscience and Biotechnology and Frontier Collaborative Research Center, Tokyo Institute of Technology, Yokohama, Japan 226-8501', 'institution_ids': ['https://openalex.org/I114531698']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5034882455', 'display_name': 'Hiroshi Handa', 'orcid': 'https://orcid.org/0000-0001-6656-9521'}, 'institutions': [{'id': 'https://openalex.org/I114531698', 'display_name': 'Tokyo Institute of Technology', 'ror': 'https://ror.org/0112mx960', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I114531698']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Hiroshi Handa', 'raw_affiliation_strings': ['Faculty of Bioscience and Biotechnology and Frontier Collaborative Research Center, Tokyo Institute of Technology, Yokohama, Japan 226-8501'], 'affiliations': [{'raw_affiliation_string': 'Faculty of Bioscience and Biotechnology and Frontier Collaborative Research Center, Tokyo Institute of Technology, Yokohama, Japan 226-8501', 'institution_ids': ['https://openalex.org/I114531698']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5074762963', 'display_name': 'David H. Price', 'orcid': 'https://orcid.org/0000-0002-5597-385X'}, 'institutions': [{'id': 'https://openalex.org/I126307644', 'display_name': 'University of Iowa', 'ror': 'https://ror.org/036jqmy94', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I126307644']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'David H. Price', 'raw_affiliation_strings': ['Department of Biochemistry, University of Iowa, Iowa City, Iowa, 52242'], 'affiliations': [{'raw_affiliation_string': 'Department of Biochemistry, University of Iowa, Iowa City, Iowa, 52242', 'institution_ids': ['https://openalex.org/I126307644']}]}], 'institution_assertions': [], 'countries_distinct_count': 2, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'apc_paid': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'fwci': 3.672, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 186, 'citation_normalized_percentile': {'value': 0.944384, 'is_in_top_1_percent': False, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 97, 'max': 98}, 'biblio': {'volume': '276', 'issue': '45', 'first_page': '42601', 'last_page': '42609'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10725', 'display_name': 'RNA Interference and Gene Delivery', 'score': 0.9991, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10725', 'display_name': 'RNA Interference and Gene Delivery', 'score': 0.9991, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10222', 'display_name': 'Genomics and Chromatin Dynamics', 'score': 0.999, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10613', 'display_name': 'Virus-based gene therapy research', 'score': 0.9976, 'subfield': {'id': 'https://openalex.org/subfields/1311', 'display_name': 'Genetics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/rna-polymerase-ii', 'display_name': 'RNA polymerase II', 'score': 0.83515227}, {'id': 'https://openalex.org/keywords/elongation-factor', 'display_name': 'Elongation factor', 'score': 0.76662004}, {'id': 'https://openalex.org/keywords/transcription', 'display_name': 'Transcription', 'score': 0.59135246}, {'id': 'https://openalex.org/keywords/p-tefb', 'display_name': 'P-TEFb', 'score': 0.5458477}, {'id': 'https://openalex.org/keywords/transcription-preinitiation-complex', 'display_name': 'Transcription preinitiation complex', 'score': 0.42753863}], 'concepts': [{'id': 'https://openalex.org/C154816499', 'wikidata': 'https://www.wikidata.org/wiki/Q21120535', 'display_name': 'Transcription factor II F', 'level': 5, 'score': 0.85610354}, {'id': 'https://openalex.org/C64350747', 'wikidata': 'https://www.wikidata.org/wiki/Q15334993', 'display_name': 'RNA polymerase II', 'level': 5, 'score': 0.83515227}, {'id': 'https://openalex.org/C139124968', 'wikidata': 'https://www.wikidata.org/wiki/Q126252', 'display_name': 'Elongation factor', 'level': 5, 'score': 0.76662004}, {'id': 'https://openalex.org/C172768829', 'wikidata': 'https://www.wikidata.org/wiki/Q14817959', 'display_name': 'Transcription factor II D', 'level': 5, 'score': 0.6798827}, {'id': 'https://openalex.org/C196117886', 'wikidata': 'https://www.wikidata.org/wiki/Q21110340', 'display_name': 'RNA polymerase II holoenzyme', 'level': 5, 'score': 0.60683775}, {'id': 'https://openalex.org/C179926584', 'wikidata': 'https://www.wikidata.org/wiki/Q207714', 'display_name': 'Transcription (linguistics)', 'level': 2, 'score': 0.59135246}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.5689688}, {'id': 'https://openalex.org/C2777841055', 'wikidata': 'https://www.wikidata.org/wiki/Q15314917', 'display_name': 'P-TEFb', 'level': 5, 'score': 0.5458477}, {'id': 'https://openalex.org/C82381507', 'wikidata': 'https://www.wikidata.org/wiki/Q416878', 'display_name': 'Polymerase', 'level': 3, 'score': 0.49281505}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.47813737}, {'id': 'https://openalex.org/C188740287', 'wikidata': 'https://www.wikidata.org/wiki/Q423333', 'display_name': 'Transcription preinitiation complex', 'level': 5, 'score': 0.42753863}, {'id': 'https://openalex.org/C67705224', 'wikidata': 'https://www.wikidata.org/wiki/Q11053', 'display_name': 'RNA', 'level': 3, 'score': 0.3802837}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.33182454}, {'id': 'https://openalex.org/C41258723', 'wikidata': 'https://www.wikidata.org/wiki/Q2919111', 'display_name': 'RNA-dependent RNA polymerase', 'level': 4, 'score': 0.24039108}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.2051371}, {'id': 'https://openalex.org/C552990157', 'wikidata': 'https://www.wikidata.org/wiki/Q7430', 'display_name': 'DNA', 'level': 2, 'score': 0.20215249}, {'id': 'https://openalex.org/C101762097', 'wikidata': 'https://www.wikidata.org/wiki/Q224093', 'display_name': 'Promoter', 'level': 4, 'score': 0.15621325}, {'id': 'https://openalex.org/C150194340', 'wikidata': 'https://www.wikidata.org/wiki/Q26972', 'display_name': 'Gene expression', 'level': 3, 'score': 0.09477705}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.058582217}, {'id': 'https://openalex.org/C88478588', 'wikidata': 'https://www.wikidata.org/wiki/Q42244', 'display_name': 'Ribosome', 'level': 4, 'score': 0.0}, {'id': 'https://openalex.org/C41895202', 'wikidata': 'https://www.wikidata.org/wiki/Q8162', 'display_name': 'Linguistics', 'level': 1, 'score': 0.0}, {'id': 'https://openalex.org/C138885662', 'wikidata': 'https://www.wikidata.org/wiki/Q5891', 'display_name': 'Philosophy', 'level': 0, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D009687', 'descriptor_name': 'Nuclear Proteins', 'qualifier_ui': 'Q000502', 'qualifier_name': 'physiology', 'is_major_topic': True}, {'descriptor_ui': 'D017346', 'descriptor_name': 'Protein-Serine-Threonine Kinases', 'qualifier_ui': 'Q000502', 'qualifier_name': 'physiology', 'is_major_topic': True}, {'descriptor_ui': 'D012319', 'descriptor_name': 'RNA Polymerase II', 'qualifier_ui': 'Q000502', 'qualifier_name': 'physiology', 'is_major_topic': True}, {'descriptor_ui': 'D014157', 'descriptor_name': 'Transcription Factors', 'qualifier_ui': 'Q000502', 'qualifier_name': 'physiology', 'is_major_topic': True}, {'descriptor_ui': 'D019473', 'descriptor_name': 'Transcription Factors, TFII', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D014158', 'descriptor_name': 'Transcription, Genetic', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D009687', 'descriptor_name': 'Nuclear Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009687', 'descriptor_name': 'Nuclear Proteins', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': False}, {'descriptor_ui': 'D042925', 'descriptor_name': 'Positive Transcriptional Elongation Factor B', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017346', 'descriptor_name': 'Protein-Serine-Threonine Kinases', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012319', 'descriptor_name': 'RNA Polymerase II', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014157', 'descriptor_name': 'Transcription Factors', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014157', 'descriptor_name': 'Transcription Factors', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': False}, {'descriptor_ui': 'D035602', 'descriptor_name': 'Transcriptional Elongation Factors', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m104967200', 'pdf_url': 'http://www.jbc.org/article/S0021925819831277/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/11553615', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m104967200', 'pdf_url': 'http://www.jbc.org/article/S0021925819831277/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 55, 'referenced_works': ['https://openalex.org/W1564623388', 'https://openalex.org/W1571663274', 'https://openalex.org/W1577893399', 'https://openalex.org/W1586160554', 'https://openalex.org/W1760846735', 'https://openalex.org/W1813910892', 'https://openalex.org/W1925058512', 'https://openalex.org/W1964408761', 'https://openalex.org/W1964670699', 'https://openalex.org/W1973000551', 'https://openalex.org/W1973604371', 'https://openalex.org/W1976210757', 'https://openalex.org/W1977121504', 'https://openalex.org/W2003168089', 'https://openalex.org/W2007883157', 'https://openalex.org/W2012475101', 'https://openalex.org/W2013824955', 'https://openalex.org/W2020329201', 'https://openalex.org/W2033680244', 'https://openalex.org/W2035895518', 'https://openalex.org/W2036580178', 'https://openalex.org/W2046336423', 'https://openalex.org/W2046854154', 'https://openalex.org/W2048816400', 'https://openalex.org/W2058565051', 'https://openalex.org/W2062654525', 'https://openalex.org/W2067953727', 'https://openalex.org/W2068479879', 'https://openalex.org/W2069497589', 'https://openalex.org/W2074637788', 'https://openalex.org/W2079489048', 'https://openalex.org/W2080993511', 'https://openalex.org/W2083804862', 'https://openalex.org/W2084544240', 'https://openalex.org/W2092594895', 'https://openalex.org/W2094924575', 'https://openalex.org/W2101424708', 'https://openalex.org/W2103626942', 'https://openalex.org/W2105721989', 'https://openalex.org/W2108422586', 'https://openalex.org/W2109240261', 'https://openalex.org/W2116384789', 'https://openalex.org/W2119141376', 'https://openalex.org/W2121414885', 'https://openalex.org/W2126730534', 'https://openalex.org/W2128431731', 'https://openalex.org/W2141373343', 'https://openalex.org/W2147347134', 'https://openalex.org/W2148330261', 'https://openalex.org/W2148917483', 'https://openalex.org/W2158563985', 'https://openalex.org/W2161932414', 'https://openalex.org/W2162082053', 'https://openalex.org/W2167931447', 'https://openalex.org/W2171171901'], 'related_works': ['https://openalex.org/W812958809', 'https://openalex.org/W4232925550', 'https://openalex.org/W2344189114', 'https://openalex.org/W2051598492', 'https://openalex.org/W2048404380', 'https://openalex.org/W2026537834', 'https://openalex.org/W2012120921', 'https://openalex.org/W2004796688', 'https://openalex.org/W1996183389', 'https://openalex.org/W145414348'], 'abstract_inverted_index': {'Studying': [0, 136], 'the': [1, 6, 13, 22, 56, 75, 77, 85, 129, 137, 142, 149, 158, 192, 211, 213, 221, 265, 314, 371, 375, 387, 437, 507, 511, 520, 703, 711, 958, 970, 1005, 1120, 1126, 1206, 1215, 1236, 1292, 1321, 1376, 1380, 1410, 1425, 1501, 1510, 1565, 1603, 1615, 1681, 1696, 1713, 1767, 1780, 1838, 1852, 1893, 1906, 1917, 2025, 2086, 2090, 2094, 2106, 2111, 2116, 2128, 2137, 2292, 2301, 2304, 2312, 2386, 2391, 2427, 2571, 2602, 2605, 2613, 2619, 2637, 2656, 2718, 2735, 2741, 2772, 2779, 2787, 2801, 2937, 2948, 2973, 3078, 3102, 3112, 3166, 3175, 3201, 3220, 3232, 3315, 3326, 3329, 3344, 3350, 3466, 3474, 3487, 3506, 3522, 3607, 3628, 3638, 3642, 3656, 3664, 3670, 3676, 3690, 3704, 3722, 3748, 3773, 3814, 3837, 3859, 3869, 3886, 3902, 3915, 3929, 3932, 3945, 3950, 3995, 4000, 4009, 4016, 4029, 4035, 4038, 4059, 4062, 4078, 4100, 4110, 4114, 4118, 4163, 4204, 4207, 4211, 4214, 4223, 4232, 4238, 4249, 4252, 4260, 4263, 4294, 4301, 4321, 4330, 4366, 4382, 4402, 4441, 4444, 4451, 4479, 4491, 4511, 4552, 4556, 4588, 4614, 4655, 4660, 4663, 4677, 4691, 4702, 4718], 'sensitivity': [2, 138, 599, 972, 1018, 1076, 1503, 1561, 1617, 1687, 1771, 1895, 1908, 3512], 'of': [3, 15, 18, 24, 41, 55, 88, 132, 139, 151, 154, 160, 177, 191, 224, 268, 291, 302, 316, 352, 373, 596, 629, 632, 693, 949, 960, 973, 996, 1002, 1007, 1119, 1205, 1212, 1372, 1379, 1409, 1417, 1427, 1495, 1504, 1512, 1554, 1567, 1594, 1605, 1618, 1644, 1698, 1702, 1707, 1716, 1718, 1759, 1769, 1782, 1822, 1827, 1837, 1886, 1896, 1902, 1919, 2027, 2089, 2105, 2113, 2130, 2133, 2139, 2190, 2294, 2303, 2324, 2329, 2364, 2368, 2447, 2482, 2544, 2548, 2573, 2589, 2604, 2639, 2663, 2743, 2778, 2786, 2800, 2809, 2818, 2830, 2856, 2879, 2890, 2947, 3012, 3018, 3023, 3080, 3091, 3104, 3151, 3177, 3204, 3223, 3234, 3237, 3288, 3314, 3328, 3352, 3378, 3402, 3468, 3476, 3491, 3524, 3605, 3641, 3659, 3663, 3672, 3699, 3706, 3719, 3724, 3747, 3781, 3787, 3825, 3836, 3862, 3867, 3888, 3904, 3994, 4011, 4025, 4028, 4086, 4099, 4113, 4120, 4168, 4191, 4206, 4222, 4235, 4262, 4303, 4332, 4341, 4346, 4358, 4363, 4387, 4401, 4427, 4443, 4453, 4463, 4468, 4481, 4493, 4523, 4535, 4538, 4558, 4578, 4587, 4592, 4601, 4617, 4626, 4647, 4652, 4657, 4662, 4680, 4693, 4704, 4708], 'transcription': [4, 25, 70, 140, 161, 206, 317, 341, 381, 603, 868, 1081, 1428, 1776, 1965, 3298, 3396, 3516, 3643, 4284], 'to': [5, 12, 47, 141, 148, 183, 349, 453, 516, 710, 866, 964, 999, 1011, 1073, 1227, 1424, 1516, 1571, 1609, 1679, 1779, 1834, 1909, 1976, 2084, 2115, 2425, 2564, 2586, 2624, 2728, 2805, 2919, 2955, 2972, 2976, 3085, 3106, 3206, 3211, 3217, 3307, 3317, 3339, 3405, 3411, 3446, 3482, 3521, 3669, 3701, 3830, 3971, 4292, 4310, 4409, 4541, 4575, 4638, 4665], 'nucleotide': [7, 143, 288, 712], 'analog': [8, 144, 713], '5,6-dichloro-1-β-d-ribofuranosylbenzimidazole': [9, 58, 145, 194, 272, 279, 597], 'has': [10, 146, 333, 1197, 1224, 3408], 'led': [11, 147, 1778], 'discovery': [14, 150, 1781], 'a': [16, 34, 93, 102, 152, 170, 229, 238, 311, 591, 630, 859, 897, 1078, 1154, 1198, 1233, 1557, 1703, 1773, 1783, 1848, 1913, 1983, 2058, 2062, 2068, 2297, 2322, 2341, 2365, 2433, 2519, 2545, 2560, 2579, 2703, 2730, 2806, 2815, 2897, 2916, 2952, 2957, 2982, 3074, 3321, 3460, 3477, 3660, 3686, 3696, 3713, 3735, 3855, 3898, 3921, 4071, 4393, 4506, 4576, 4585], 'number': [17, 153], 'proteins': [19, 44, 155, 180, 1423, 3873, 3882, 4074], 'involved': [20, 156, 1755], 'in': [21, 157, 506, 600, 601, 900, 1077, 1214, 1291, 1709, 1756, 1772, 2032, 2056, 2067, 2127, 2240, 2284, 2314, 2350, 2370, 2395, 2504, 2550, 2601, 2636, 2648, 2792, 2993, 3148, 3153, 3160, 3174, 3296, 3300, 3369, 3395, 3459, 3473, 3486, 3495, 3505, 3514, 3637, 3737, 3795, 3807, 3858, 3875, 3885, 3901, 3988, 3999, 4008, 4034, 4182, 4186, 4203, 4231, 4248, 4272, 4279, 4281, 4320, 4329, 4369, 4412, 4450, 4515, 4555, 4564, 4590, 4642, 4690], 'regulation': [23, 159, 1426], 'elongation': [26, 37, 52, 64, 71, 109, 162, 173, 188, 200, 207, 245, 283, 318, 342, 379, 444, 508, 515, 522, 691, 706, 869, 1295, 1377, 1429, 1651, 1787, 1987, 2033, 2042, 2049, 2087, 2103, 2989, 3002, 3007, 3096, 3155, 3197, 3289, 3310, 3331, 3414, 3444, 3457, 3472, 3484, 3609, 3639, 3657, 3720, 3741, 3750, 3782, 4296, 4313, 4383, 4524, 4640], 'by': [27, 73, 110, 163, 209, 246, 386, 442, 895, 1385, 2052, 2092, 2110, 2198, 2310, 2655, 2894, 2943, 3101, 3163, 3200, 3231, 3291, 3381, 3745, 3842, 3912, 4044, 4418, 4435, 4498, 4525, 4573, 4701], 'RNA': [28, 49, 164, 185, 346, 961, 1008, 1221, 1323, 1383, 1513, 1568, 1606, 1844, 1927, 2039, 3255, 3357, 3469], 'polymerase': [29, 50, 78, 165, 186, 214, 347, 376, 438, 962, 1009, 1222, 1324, 1384, 1514, 1569, 1607, 1928, 2040, 2091, 2107, 2944, 3358, 3470, 4554, 4664], 'II.': [30, 166], 'We': [31, 113, 167, 249, 2076, 2118, 2568, 3463, 4258, 4406], 'have': [32, 168, 1419, 3683, 4228], 'developed': [33, 169, 3294], 'highly': [35, 171, 1199, 2069, 3449], 'purified': [36, 43, 172, 179, 585, 997, 1068, 1080, 1645, 1775, 1903, 1926, 2070, 2074, 2237, 2483, 2574, 2769, 3172, 3450, 3799, 3896, 3907, 4043, 4305, 4308], 'control': [38, 174, 301, 315, 509, 1296, 1652, 2050, 3290, 3415, 3458], 'system': [39, 175, 1082, 3299, 3736], 'composed': [40, 176, 1118], 'three': [42, 178, 3118], 'added': [45, 181, 1975, 2424, 3305, 3480, 4375, 4391, 4471, 4504, 4636, 4695], 'back': [46, 182, 3306, 3481, 4637], 'isolated': [48, 184, 2038, 3167, 3308, 3443, 4013, 4036, 4311, 4322, 4639], 'II': [51, 187, 348, 963, 1010, 1223, 1515, 1570, 1608, 1929, 2041, 3359, 3471], 'complexes.': [53, 189, 3311, 3332], 'Two': [54, 190], 'proteins,': [57, 193], 'sensitivity-inducing': [59, 195, 280, 1064], 'factor': [60, 65, 105, 196, 201, 241, 281, 284, 523, 592, 1058, 1065, 1788, 4577], '(DSIF)': [61, 197, 2515], 'and': [62, 90, 121, 123, 134, 198, 226, 257, 259, 270, 296, 380, 451, 637, 701, 705, 968, 1015, 1125, 1159, 1209, 1219, 1325, 1332, 1406, 1414, 1562, 1597, 1647, 1688, 1832, 1847, 1922, 1972, 2030, 2066, 2080, 2143, 2188, 2193, 2225, 2277, 2373, 2390, 2464, 2485, 2507, 2513, 2542, 2583, 2595, 2616, 2634, 2671, 2696, 2700, 2717, 2775, 2812, 2847, 2851, 2913, 2998, 3020, 3036, 3083, 3088, 3127, 3140, 3146, 3189, 3209, 3228, 3257, 3361, 3376, 3390, 3393, 3453, 3721, 3740, 3790, 3798, 3805, 3871, 3914, 3931, 3959, 3978, 3986, 4003, 4070, 4095, 4104, 4172, 4178, 4268, 4307, 4343, 4360, 4420, 4429, 4455, 4465, 4474, 4619, 4649], 'negative': [63, 69, 86, 199, 205, 222, 282, 297, 340, 388, 1682, 1691, 1705, 1786, 2298, 4295, 4394, 4615], '(NELF),': [66, 202], 'act': [67, 203, 344], 'as': [68, 204, 447, 590, 903, 912, 2238, 2781, 3493, 3800, 3981], 'factors': [72, 208, 298, 343, 389, 445, 1764, 3173, 3302, 3380, 4389, 4437], 'increasing': [74, 210, 2093, 4624], 'time': [76, 212, 2095, 2578, 3224, 3631, 3652, 3700], 'spent': [79, 215], 'at': [80, 216, 1851, 2098, 2281, 2379, 2414, 2555, 2612, 2710, 2930, 3043, 3225, 3712, 3936, 3955, 4054, 4106, 4244, 4439, 4505], 'pause': [81, 217, 2099], 'sites.': [82, 218, 2100], 'P-TEFb': [83, 219, 374, 524, 582, 627, 694, 890, 950, 998, 1555, 1598, 1648, 1677, 2031, 2053, 2114, 2191, 3454, 3525, 3673, 3694, 3725, 4627, 4658, 4681, 4709], 'reverses': [84, 220], 'effect': [87, 223, 1918, 2299, 4302, 4395, 4550], 'DSIF': [89, 120, 225, 256, 1116, 1496, 1596, 1646, 1708, 1753, 1921, 1971, 2079, 2553, 2575, 3933, 4306, 4342, 4372, 4419, 4428, 4454, 4464, 4482, 4502, 4565, 4589, 4618, 4648], 'NELF': [91, 227, 1820, 1904, 1923, 1973, 2081, 2122, 2607, 2767, 2873, 2891, 2927, 3947, 3996, 4012, 4039, 4052, 4064, 4079, 4093, 4115, 4125, 4192, 4265, 4309, 4347, 4359, 4374, 4421, 4456, 4469, 4484, 4539, 4602, 4620], 'through': [92, 228, 1164, 1232, 3320, 3928], 'mechanism': [94, 230], 'dependent': [95, 127, 231, 263, 696, 1694], 'on': [96, 128, 232, 264, 697, 1070, 1695, 1982, 2300, 2896, 3259, 3343, 3680, 4046, 4193, 4381, 4398, 4551, 4683], 'its': [97, 233, 698, 864, 1071, 3956], 'kinase': [98, 234, 634, 699, 704, 860, 3677], 'activity.': [99, 235], 'TFIIF': [100, 116, 133, 236, 252, 269, 2126, 2235], 'is': [101, 126, 237, 262, 382, 439, 891, 1117, 1326, 1693, 1754, 2150, 3335, 3502, 3518, 3828, 4581], 'general': [103, 239, 443, 1757], 'initiation': [104, 240, 3739], 'that': [106, 115, 242, 251, 309, 336, 907, 974, 1505, 1619, 1676, 1689, 1752, 1897, 1978, 2078, 2121, 2570, 3510, 3675, 3682, 3693, 3772, 3823, 4075, 4162, 4201, 4270, 4432, 4495, 4567, 4571, 4583], 'positively': [107, 243], 'affects': [108, 244], 'decreasing': [111, 247], 'pausing.': [112, 248], 'show': [114, 250, 2120], 'functionally': [117, 253, 2123], 'competes': [118, 254, 2124], 'with': [119, 255, 1162, 1230, 1685, 1925, 2125, 2145, 2273, 2336, 2347, 2443, 2466, 2477, 2524, 2669, 2827, 2853, 2876, 2906, 2909, 2915, 2922, 2951, 2965, 2981, 3027, 3077, 3111, 3120, 3132, 3169, 3337, 3384, 3645, 3813, 3846, 4077, 4124, 4210, 4299, 4349, 4676], 'NELF,': [122, 258, 2029, 3452, 4236, 4430], 'this': [124, 260, 435, 1690, 2047, 4370], 'competition': [125, 261], 'relative': [130, 266], 'concentrations': [131, 267, 4367, 4426, 4480], 'NELF.': [135, 271, 4536, 4653], 'human': [273, 626, 2141, 2234, 3826], 'immunodeficiency': [274], 'virus': [275], 'HeLa': [276, 952, 1710, 2063, 2629, 2731, 3963], 'nuclear': [277, 588, 953, 2064, 2724, 2732, 2747, 3403, 3478, 3979, 4019], 'extract': [278, 954, 975, 1506, 1601, 1620, 2065, 2733, 2748, 3404, 3479], 'dithiothreitol': [285], 'phenylmethylsulfonyl': [286], 'fluoride': [287], 'The': [289, 503, 690, 994, 1700, 1761, 1825, 2101, 2375, 2409, 2439, 2706, 2723, 2784, 2794, 2823, 2902, 2926, 2961, 3312, 3333, 3371, 3400, 3717, 3767, 3784, 3833, 3865, 3906, 3992, 4084, 4673], 'balance': [290], 'activity': [292, 1683, 1692, 2572, 4094, 4126, 4261, 4280, 4297, 4532, 4616], 'between': [293], 'both': [294, 702, 1595, 2057, 2140, 2771, 3793, 3868, 3881, 3976, 4386], 'positive': [295, 521], 'achieves': [299], 'accurate': [300], 'many': [303], 'cellular': [304], 'processes.': [305], 'Accumulating': [306], 'evidence': [307], 'indicates': [308, 3509, 4161], 'such': [310, 446, 3406], 'process': [312], 'regulates': [313], '(1Price': [319, 525, 3526], 'D.H.': [320, 358, 393, 417, 526, 541, 565, 608, 653, 671, 718, 742, 766, 794, 824, 842, 931, 983, 1026, 1582, 2209, 2253, 2750, 3055, 3267, 3425, 3527, 3546, 3570, 3586, 3755], 'Mol.': [321, 359, 527, 1275, 3528, 3756], 'Cell.': [322, 360, 528, 1276, 1358, 1806, 1871, 1948, 2008, 3529, 3757, 4143], 'Biol.': [323, 361, 395, 419, 494, 529, 543, 567, 610, 675, 720, 744, 844, 879, 933, 1181, 1252, 1277, 1344, 1480, 1736, 2259, 2756, 3061, 3273, 3431, 3530, 3548, 3588, 3758], '2000;': [324, 530, 935, 1278, 1467, 3531], '20:': [325, 531, 1279, 3532], '2629-2634Crossref': [326, 532, 3533], 'PubMed': [327, 365, 405, 429, 468, 480, 498, 533, 553, 577, 620, 659, 685, 730, 754, 772, 800, 832, 854, 887, 943, 989, 1032, 1052, 1111, 1147, 1191, 1262, 1281, 1314, 1352, 1365, 1402, 1443, 1470, 1489, 1548, 1588, 1636, 1668, 1746, 1815, 1880, 1957, 2017, 2181, 2215, 2263, 2764, 3065, 3281, 3435, 3534, 3558, 3576, 3598, 3762, 4152], 'Scopus': [328, 366, 406, 430, 469, 481, 499, 534, 554, 578, 621, 660, 686, 731, 755, 773, 801, 833, 855, 944, 990, 1033, 1053, 1112, 1148, 1192, 1263, 1282, 1315, 1366, 1444, 1471, 1490, 1549, 1589, 1637, 1669, 1747, 1816, 1881, 1958, 2018, 2182, 2216, 2264, 3066, 3436, 3535, 3559, 3577, 3599, 3763, 4153], '(571)': [329, 535, 1113, 1550, 2183, 3536], 'Google': [330, 368, 408, 432, 471, 483, 501, 536, 556, 580, 623, 662, 688, 733, 757, 775, 803, 835, 857, 888, 946, 992, 1035, 1055, 1114, 1150, 1194, 1265, 1284, 1317, 1353, 1368, 1403, 1446, 1473, 1492, 1551, 1591, 1639, 1671, 1749, 1818, 1883, 1960, 2020, 2184, 2218, 2266, 2765, 3068, 3282, 3438, 3537, 3561, 3579, 3601, 3765, 4155], 'Scholar).': [331, 369, 433, 502, 581, 624, 689, 889, 947, 993, 1056, 1115, 1151, 1195, 1285, 1318, 1369, 1404, 1493, 1552, 1592, 1640, 1672, 1819, 1884, 1961, 2021, 2185, 2219, 2267, 2766, 3069, 3283, 3439, 3602, 3766], 'It': [332], 'been': [334, 1225, 1980, 3409, 4229], 'proposed': [335], 'shortly': [337], 'after': [338], 'initiation,': [339], 'upon': [345, 441], 'cause': [350], 'production': [351], 'short': [353], 'transcripts': [354, 456, 967, 1014, 1519, 1574, 1612, 3152, 3363, 4668, 4674], '(2Marshall': [355, 3752], 'N.F.': [356, 391, 411, 539, 559, 606, 716, 736, 840, 3540, 3584, 3753], 'Price': [357, 392, 416, 540, 564, 607, 652, 670, 717, 741, 765, 793, 823, 841, 930, 982, 1025, 1581, 2208, 2252, 3054, 3424, 3545, 3569, 3585, 3754], '1992;': [362, 1346, 1359, 3759], '12:': [363, 478, 657, 770, 987, 1030, 1109, 1441, 1546, 1586, 2179, 2213, 3574, 3760], '2078-2090Crossref': [364, 3761], '(241)': [367, 3764], 'With': [370, 2046], 'action': [372], 'enters': [377], 'productive': [378, 517], 'no': [383, 4004, 4277, 4378, 4549], 'longer': [384, 3651, 4667], 'influenced': [385], '(3Marshall': [390, 605, 715], 'J.': [394, 413, 418, 464, 476, 542, 561, 566, 609, 647, 667, 674, 719, 738, 743, 760, 782, 810, 812, 818, 838, 843, 878, 932, 977, 1020, 1048, 1141, 1177, 1180, 1248, 1251, 1272, 1336, 1342, 1343, 1397, 1457, 1576, 1632, 1664, 1732, 1735, 1803, 1868, 1945, 2005, 2203, 2245, 2249, 2755, 3047, 3051, 3272, 3417, 3421, 3542, 3547, 3564, 3582, 3587, 4140], 'Chem.': [396, 420, 544, 568, 611, 676, 721, 745, 845, 880, 934, 1182, 1253, 1345, 1737, 2757, 3274, 3549, 3589], '1995;': [397, 465, 545, 612, 722, 1399], '270:': [398, 546, 613, 723], '12335-12338Abstract': [399, 547, 614, 724], 'Full': [400, 402, 424, 426, 548, 550, 572, 574, 615, 617, 680, 682, 725, 727, 749, 751, 849, 851, 884, 938, 940, 1186, 1188, 1257, 1259, 1311, 1349, 1362, 1484, 1486, 1741, 1743, 1810, 1812, 1875, 1877, 1952, 1954, 2012, 2014, 2761, 3278, 3553, 3555, 3593, 3595, 4147, 4149], 'Text': [401, 403, 425, 427, 549, 551, 573, 575, 616, 618, 681, 683, 726, 728, 750, 752, 850, 852, 885, 939, 941, 1187, 1189, 1258, 1260, 1312, 1350, 1363, 1485, 1487, 1742, 1744, 1811, 1813, 1876, 1878, 1953, 1955, 2013, 2015, 2762, 3279, 3554, 3556, 3594, 3596, 4148, 4150], 'PDF': [404, 428, 552, 576, 619, 684, 729, 753, 853, 886, 942, 1190, 1261, 1313, 1351, 1364, 1488, 1745, 1814, 1879, 1956, 2016, 2763, 3280, 3557, 3597, 4151], '(426)': [407, 555, 622, 732], 'Scholar,': [409, 472, 484, 537, 557, 663, 734, 758, 776, 804, 836, 1036, 1266, 1447, 1474, 3538, 3562, 3580], '4Marshall': [410, 558, 735, 3539], 'Peng': [412, 560, 666, 737, 781, 817, 2199, 2241, 3541], 'Xie': [414, 562, 739, 3543], 'Z.': [415, 563, 740, 3544], '1996;': [421, 569, 746, 1308, 3550], '271:': [422, 570, 747, 3551], '27176-27183Abstract': [423, 571, 748, 3552], '(530)': [431, 579, 756, 3560], 'After': [434, 2340, 3194, 3603], 'transition,': [436], 'acted': [440], 'S-II,': [448], 'TFIIF,': [449], 'ELL,': [450], 'elongin': [452], 'generate': [454, 1012, 1517, 1572, 2625, 2977, 4666], 'long': [455, 1518, 1573, 1611], '(5Aso': [457], 'T.': [458, 780, 788, 1038, 1040, 1084, 1086, 1092, 1134, 1138, 1171, 1175, 1242, 1246, 1433, 1455, 1521, 1523, 1529, 1622, 1624, 1654, 1656, 1726, 1730, 1793, 1795, 1858, 1860, 1935, 1937, 1995, 1997, 2154, 2156, 2162, 4130, 4132], 'Conaway': [459, 461, 487, 489], 'J.W.': [460, 490], 'R.C.': [462, 488], 'FASEB': [463, 475], '9:': [466], '1419-1428Crossref': [467], '(56)': [470], '6Shilatifard': [473], 'A.': [474, 873, 1090, 1459, 1465, 1527, 1799, 1864, 1941, 2001, 2160, 3498, 3810, 3991, 4136], '1998;': [477, 656, 769, 846, 986, 1029, 1049, 1108, 1440, 1545, 1585, 1633, 1665, 2178, 2212, 2260, 3062, 3432, 3573, 3590], '1437-1446Crossref': [479], '(99)': [482], '7Reines': [485], 'D.': [486, 822, 1044, 1173, 1244, 1268, 1461, 1628, 1660, 1728], 'Curr.': [491, 1479], 'Opin.': [492], 'Cell': [493, 2658], '1999;': [495, 677, 1183, 1254, 1738, 1807, 1872, 1949, 2009, 4144], '11:': [496, 798, 830, 1482], '342-346Crossref': [497], '(69)': [500], 'key': [504], 'step': [505], 'process,': [510], 'transition': [512], 'from': [513, 586, 951, 1497, 1556, 1599, 1888, 2231, 2562, 2770, 2776, 2797, 3975, 4014, 4067], 'abortive': [514], 'elongation,': [518, 3710], 'requires': [519, 3695], '3Marshall': [538], 'was': [583, 1067, 1830, 1930, 1966, 1988, 2044, 2054, 2108, 2236, 2308, 2334, 2377, 2388, 2393, 2412, 2423, 2441, 2475, 2516, 2581, 2610, 2708, 2721, 2726, 2768, 2789, 2803, 2825, 2874, 2892, 2904, 2941, 2963, 3025, 3071, 3099, 3157, 3198, 3263, 3635, 3731, 3840, 3878, 3894, 3918, 3953, 3997, 4006, 4041, 4242, 4315, 4396, 4503, 4533, 4595, 4669, 4698], 'originally': [584], 'Drosophila': [587, 2744], 'extracts,': [589], 'required': [593, 1059, 1327, 1765], 'for': [594, 863, 1060, 1328, 1763, 1766, 2136, 2383, 2417, 2674, 2714, 2740, 2791, 3040, 3093, 3219, 3325, 3613, 4326], 'reconstitution': [595, 1768], '(DRB)1': [598], 'vitro': [602, 3297], 'assays': [604, 3517], 'Active': [625], 'consists': [628, 1821], 'heterodimer': [631, 3934], 'cyclin-dependent': [633], '9': [635], '(Cdk9)': [636], 'either': [638, 3684, 4015], 'cyclin': [639, 641, 644, 2194], 'T1,': [640], 'T2,': [642], 'or': [643, 3171, 3489, 3621, 3646, 3692, 4018, 4338, 4348, 4355, 4373, 4483, 4632], 'K': [645], '(8Peng': [646, 976, 1019, 1575, 2202], 'Zhu': [648, 761, 815, 978, 1021, 1577, 2204, 2250, 3052, 3422, 3565], 'Y.': [649, 762, 778, 784, 816, 979, 1022, 1042, 1088, 1132, 1169, 1240, 1451, 1525, 1578, 1626, 1658, 1724, 1791, 1856, 1933, 1993, 2158, 2205, 2251, 3053, 3423, 3566, 4128, 4176], 'Milton': [650, 763, 980, 1023, 1579, 2206, 3567], 'J.T.': [651, 764, 981, 1024, 1580, 2207, 3568], 'Genes': [654, 767, 795, 827, 984, 1027, 1106, 1438, 1543, 1583, 2176, 2210, 3571], 'Dev.': [655, 768, 796, 828, 985, 1028, 1107, 1439, 1544, 1584, 2177, 2211, 3572], '755-762Crossref': [658, 771, 988, 1031, 1587, 2214, 3575], '(451)': [661, 774, 991, 1034, 1590, 2217, 3578], '9Fu': [664], 'T.J.': [665], 'Lee': [668, 807, 1456], 'G.': [669, 808], 'Flores': [672, 825], 'O.': [673, 826], '274:': [678, 1184, 1255, 1739], '34527-34530Abstract': [679], '(179)': [687], 'properties': [692, 4010], 'are': [695, 708, 1288, 2196, 3374], 'activity,': [700], 'activities': [707, 1334, 4570, 4607], 'sensitive': [709], 'DRB': [714, 971, 1017, 1061, 1063, 1075, 1502, 1560, 1616, 1686, 1770, 1894, 1907, 3039, 3492, 3511, 3648, 3654], '8Peng': [759, 3563], '10Zhu': [777], "Pe'ery": [779], 'Ramanathan': [783], 'Marshall': [785, 787, 839, 3583], 'N.': [786, 1136], 'Amendt': [789], 'B.': [790], 'Mathews': [791], 'M.B.': [792], '1997;': [797, 829], '2622-2632Crossref': [799], '(613)': [802], '11Mancebo': [805], 'H.S.': [806], 'Flygare': [809], 'Tomassini': [811], 'Luu': [813], 'P.': [814], 'Blau': [819], 'C.': [820], 'Hazuda': [821], '2633-2644Crossref': [831], '(480)': [834], '12Peng': [837, 3581], '273:': [847, 3591], '13855-13860Abstract': [848, 3592], '(151)': [856, 3600], 'Scholar),': [858, 1750, 4156], 'inhibitor': [861], 'known': [862], 'ability': [865, 959, 1006, 1072, 1511, 1566, 1604, 4661], 'inhibit': [867], '(13Chodosh': [870], 'L.A.': [871], 'Fire': [872], 'Samuels': [874], 'M.': [875, 2247, 3049, 3419], 'Sharp': [876], 'P.A.': [877], '1989;': [881], '264:': [882], '2250-2257Abstract': [883], 'also': [892, 909, 1289, 1374, 1420, 1989, 2119, 4096], 'strongly': [893, 3508], 'inhibited': [894], 'flavopiridol,': [896], 'drug': [898], 'currently': [899], 'clinical': [901], 'trials': [902], 'an': [904, 913, 1600, 1843, 1889, 1900, 2035, 2146, 2327, 2478, 2978, 3295, 4026, 4245, 4290], 'anti-cancer': [905], 'treatment': [906], 'might': [908], 'be': [910, 3304, 4256, 4622, 4715], 'useful': [911], 'anti-HIV': [914], 'therapy': [915], '(14Chao': [916], 'S.H.': [917], 'Fujinaga': [918], 'K.': [919, 1097, 1534, 1797, 1862, 1939, 1999, 2167, 4134], 'Marion': [920, 2248, 3050, 3420], 'J.E.': [921], 'Taube': [922], 'R.': [923, 1338], 'Sausville': [924], 'E.A.': [925], 'Senderowicz': [926], 'A.M.': [927], 'Peterlin': [928], 'B.M.': [929], '275:': [936], '28345-28348Abstract': [937], '(431)': [945], 'Depletion': [948, 1494, 1553], '(HNE)': [955], 'greatly': [956, 1499, 1563], 'reduces': [957, 1500, 1564], 'produce': [965, 2956], 'full-length': [966, 1013], 'eliminates': [969, 1559], 'addition': [995, 2112, 3079, 3103, 3203, 3233, 3351, 3401, 4703], 'HNE': [1000, 1498, 1558, 1890, 2780, 2788, 3024, 3170, 3338], 'depleted': [1001], 'Cdk9': [1003], 'restores': [1004, 1016, 1602], '15Wada': [1037], 'Takagi': [1039, 1085, 1174, 1245, 1522, 1623, 1655, 1729, 1792, 1857, 1934, 1994, 2155, 4129], 'Yamaguchi': [1041, 1087, 1450, 1524, 1625, 1657, 2157], 'Watanabe': [1043, 1172, 1243, 1627, 1659, 1727], 'Handa': [1045, 1104, 1139, 1178, 1249, 1434, 1462, 1541, 1629, 1661, 1733, 1804, 1869, 1946, 2006, 2174, 4141], 'H.': [1046, 1105, 1140, 1179, 1250, 1435, 1463, 1542, 1630, 1662, 1734, 1805, 1870, 1947, 2007, 2175, 4142, 4179], 'EMBO': [1047, 1631, 1663], '17:': [1050, 1634, 1666], '7395-7403Crossref': [1051, 1635, 1667], '(281)': [1054, 1638, 1670], 'Another': [1057], 'sensitivity,': [1062], '(DSIF),': [1066], 'based': [1069], 'reconstitute': [1074, 3412, 3456, 4293], 'partially': [1079, 1774, 4196], '(16Wada': [1083, 1520, 2153], 'Ferdous': [1089, 1526, 2159], 'Imai': [1091, 1528, 2161], 'Hirose': [1093, 1530, 2163], 'S.': [1094, 1103, 1340, 1449, 1453, 1531, 1540, 1801, 1866, 1943, 2003, 2164, 2173, 4138], 'Sugimoto': [1095, 1532, 1800, 1865, 1942, 2002, 2165, 4137], 'Yano': [1096, 1533, 1796, 1861, 1938, 1998, 2166, 4133], 'Hartzog': [1098, 1535, 2168], 'G.A.': [1099, 1431, 1536, 2169], 'Winston': [1100, 1436, 1537, 2170], 'F.': [1101, 1437, 1538, 2171], 'Buratowski': [1102, 1539, 2172], '343-356Crossref': [1110, 1547, 2180], '14-kDa': [1121], 'Spt4': [1122, 1152, 2142, 2276, 2484, 2512, 3789, 3870, 3926], 'subunit': [1123, 1129, 3893, 4033, 4209, 4253], '(p14)': [1124], '120-kDa': [1127], 'Spt5': [1128, 1163, 1196, 1231, 1418, 2144, 2278, 2295, 2474, 2514, 3815, 3827, 3872], '(p160)': [1130], '(17Yamaguchi': [1131], 'Narita': [1133], 'Inukai': [1135], 'Wada': [1137, 1170, 1241, 1432, 1454, 1725, 1794, 1859, 1936, 1996, 4131], 'Biochem.': [1142, 1306], '(Tokyo).': [1143], '2001;': [1144, 1481], '129:': [1145], '185-191Crossref': [1146], '(43)': [1149], 'possesses': [1153], 'putative': [1155], 'zinc': [1156], 'finger': [1157], 'domain': [1158], 'probably': [1160], 'interacts': [1161], 'mainly': [1165], 'hydrophobic': [1166], 'interactions': [1167], '(18Yamaguchi': [1168, 1239, 1723], 'Hasegawa': [1176, 1247, 1731, 1802, 1867, 1944, 2004, 4139], '8085-8092Abstract': [1185, 1256, 1740], '(117)': [1193, 1264, 1748], 'acidic': [1200], 'N-terminal': [1201, 2147], 'region,': [1202], 'four': [1203, 1719, 1823], 'copies': [1204], 'KOW': [1207, 1237, 1286], 'repeat,': [1208], 'two': [1210, 3130, 3785, 4167, 4198, 4224, 4264], 'sets': [1211], 'repeats': [1213, 1238, 1287, 1836], 'C-terminal': [1216, 1853], 'region': [1217, 1234], '(CTR1': [1218], 'CTR2).': [1220], 'shown': [1226], 'directly': [1228], 'interact': [1229], 'encompassing': [1235], '19Ivanov': [1267], 'Kwak': [1269], 'Y.T.': [1270], 'Guo': [1271], 'Gaynor': [1273], 'R.B.': [1274], '2970-2983Crossref': [1280], '(177)': [1283], 'found': [1290, 1833, 2077, 2569, 3732, 4276], 'Escherichia': [1293], 'coli': [1294, 1382, 2269, 3797, 3877], 'protein': [1297, 4101, 4158], 'NusG': [1298, 1319, 1373], '(20Kyrpides': [1299], 'N.C.': [1300], 'Woese': [1301], 'C.R.': [1302], 'Ouzounis': [1303], 'C.A.': [1304], 'Trends': [1305], 'Sci.': [1307], '21:': [1309], '425-426Abstract': [1310], '(112)': [1316], 'binds': [1320], 'prokaryotic': [1322], 'certain': [1329, 3687, 3697], 'specific': [1330, 4569, 4606], 'termination': [1331], 'anti-termination': [1333], '(21Li': [1335], 'Horwitz': [1337], 'McCracken': [1339], 'Greenblatt': [1341], '267:': [1347], '6012-6019Abstract': [1348], 'Scholar,22Sullivan': [1354], 'S.L.': [1355], 'Gottesman': [1356, 1395], 'M.E.': [1357, 1396], '68:': [1360], '989-994Abstract': [1361], '(150)': [1367], 'Saturating': [1370], 'amounts': [1371, 3222, 4625], 'enhance': [1375], 'rate': [1378, 2088, 2104, 4442, 4452, 4522], 'E.': [1381, 1388, 2268, 3796, 3831, 3876], '∼20%': [1386, 4526], '(23Burova': [1387], 'Hung': [1389], 'S.C.': [1390], 'Sagitov': [1391], 'V.': [1392], 'Stitt': [1393], 'B.L.': [1394], 'Bacteriol.': [1398], '177:': [1400], '1388-1392Crossref': [1401], 'Genetic': [1405], 'biochemical': [1407], 'studies': [1408], 'yeast,': [1411], 'fruit': [1412], 'fly,': [1413], 'zebrafish': [1415], 'homologs': [1416], 'linked': [1421], 'these': [1422, 1963, 4169, 4388, 4436, 4486, 4499, 4593], '(24Hartzog': [1430], '357-369Crossref': [1442], '(373)': [1445], '25Guo': [1448], 'Schilbach': [1452], 'Goddard': [1458], 'French': [1460], 'Rosenthal': [1464], 'Nature.': [1466], '408:': [1468], '366-369Crossref': [1469], '(141)': [1472], '26Zorio': [1475], 'D.A.': [1476], 'Bentley': [1477], 'D.L.': [1478], 'R144-R146Abstract': [1483], '(23)': [1491], 'without': [1507, 3398, 3647], 'significantly': [1508, 1891, 1990], 'affecting': [1509], 'Immunodepletion': [1593, 1885], 'make': [1610], 'while': [1613, 1899], 'eliminating': [1614], '(15Wada': [1621, 1653], 'A': [1641, 2933, 4447], 'dose-dependent': [1642], 'add-back': [1643, 1901, 3604, 3774, 4283], 'reconstitutes': [1649, 3777], 'DRB-dependent': [1650], 'This': [1673, 3666, 3853, 4220, 4530, 4697, 4713], 'finding': [1674], 'suggests': [1675, 3674], 'functions': [1678, 3679], 'reverse': [1680], 'associated': [1684, 3110, 3379, 4076], 'presence': [1697, 2129, 2638, 3176, 3475, 3490, 3705, 3887, 3903, 4085, 4119, 4331], 'DSIF.': [1699, 2131, 4364, 4559], 'expression': [1701, 1714, 2138, 2187, 2293, 2621, 3824, 3838], 'dominant': [1704], 'form': [1706, 3340, 4205], 'cells': [1711, 2271, 2313, 2344, 2631, 2664, 2802], 'increased': [1712, 4092, 4520], 'level': [1715, 3861, 4656, 4679], 'each': [1717, 2577, 2986, 3005, 3094], 'reporter': [1720], 'genes': [1721], 'severalfold': [1722], 'suggesting': [1751, 3822], 'repression': [1758], 'transcription.': [1760], 'search': [1762], 'assay': [1777, 2036, 3775, 4285], 'third': [1784], 'factor,': [1785], '(NELF)': [1789], '(27Yamaguchi': [1790, 1855, 1932, 1992, 4127], 'Furuya': [1798, 1863, 1940, 2000, 4135], '97:': [1808, 1873, 1950, 2010, 4145], '41-51Abstract': [1809, 1874, 1951, 2011, 4146], '(622)': [1817, 1882, 1959, 2019, 4154], 'subunits.': [1824], 'smallest': [1826, 3951], 'these,': [1828], 'NELF-E,': [1829, 3949], 'cloned': [1831], 'contain': [1835], 'dipeptide': [1839], 'Arg-Asp': [1840], '(RD': [1841], 'repeats),': [1842], 'recognition': [1845], 'motif,': [1846], 'leucine': [1849], 'zipper': [1850], 'end': [1854], 'NELF-E': [1887, 2609, 4032, 4069], 'reduced': [1892, 1991, 3655], 'extract,': [1898], 'restored': [1905, 2109, 4670], 'original': [1910, 2102, 4111, 4233], 'levels.': [1911], 'Using': [1912, 4424], 'deoxycytosine-tailed': [1914], 'template': [1915, 2935, 2980, 3019, 3114, 3316, 3334, 3691], 'assay,': [1916, 4371], 'combining': [1920], 'together': [1924, 3911], 'tested': [1931, 4259, 4316, 4514], 'Under': [1962, 4058], 'conditions,': [1964], 'nearly': [1967], 'completely': [1968], 'inhibited.': [1969], 'When': [1970, 2321, 4385, 4501], 'were': [1974, 2229, 2279, 2345, 2593, 2632, 2646, 2665, 2677, 2991, 3115, 3215, 3229, 3611, 3742, 3792, 3883, 3909, 3969, 4324, 4390, 4407, 4416, 4433, 4470, 4496, 4635, 4682], 'polymerases': [1977, 3373, 4403, 4415, 4431, 4458, 4494, 4519], 'had': [1979, 2296, 4377, 4548, 4568, 4603], 'paused': [1981], 'tailed': [1984], 'template,': [1985], 'further': [1986, 2023, 3285, 4042], 'To': [2022, 2599, 3284, 3943, 4611], 'study': [2024], 'role': [2026], 'DSIF,': [2028, 3451, 3788], 'control,': [2034], 'using': [2037, 2072, 2702, 2734, 3734], 'complexes': [2043, 2990, 3156, 3168, 3342, 3407, 3445, 3485, 3610, 3644, 3681, 3708, 3751, 3974, 4200, 4314, 4323, 4641], 'utilized.': [2045], 'system,': [2048, 2060, 2071], 'mediated': [2051], 'reproduced': [2055], 'crude': [2059], 'incorporating': [2061], 'only': [2073, 2597, 3678, 4584], 'factors.': [2075, 4500, 4696], 'work': [2082], 'cooperatively': [2083], 'decrease': [2085], 'it': [2096, 2333, 4580], 'spends': [2097], 'assay.': [2117], 'Generation': [2132], 'pET': [2134, 3816], 'constructs': [2135], 'His': [2148], 'tag': [2149], 'described': [2151, 2197, 2239, 2782, 3264, 3504, 3801, 3982], 'elsewhere': [2152], 'Baculovirus': [2186], 'purification': [2189, 2603, 2889, 4091, 4103, 4112, 4190, 4234, 4250], '(Cdk9': [2192], 'T2a)': [2195], 'et': [2200, 2242], 'al.': [2201, 2243], 'Anti-FLAG': [2220, 3966], 'M2-agarose': [2221, 2820], 'affinity': [2222, 2821, 4082], 'gel': [2223, 3262], '(A-1205)': [2224], 'FLAG': [2226, 2886], 'peptide': [2227], '(F-3290)': [2228], 'obtained': [2230, 2893], 'Sigma.': [2232], 'Recombinant': [2233], '(28Peng': [2244, 3046, 3416], 'Liu': [2246, 3048, 3418], 'Cold': [2254, 3056, 3426], 'Spring': [2255, 3057, 3427], 'Harbor': [2256, 3058, 3428], 'Symp.': [2257, 3059, 3429], 'Quant.': [2258, 3060, 3430], '63:': [2261, 3063, 3433], '365-370Crossref': [2262, 3064, 3434], '(31)': [2265, 3067, 3437], 'DE3': [2270], 'transformed': [2272, 3812], 'plasmids': [2274], 'expressing': [2275], 'grown': [2280, 2647], '37': [2282], '°C': [2283], 'LB': [2285], 'medium': [2286, 2316, 2650, 3849], 'containing': [2287, 2469, 2651, 2883, 2936, 3015, 3386, 4213, 4644], '100': [2288, 2401, 2404, 2498, 2870, 2884, 3244, 4339], 'μg/ml': [2289, 2641, 2885, 3142, 3185, 3253], 'ampicillin.': [2290], 'Because': [2291, 2588, 4024, 4560], 'growth': [2302], 'cells,': [2305], 'ampicillin': [2306, 3844], 'selection': [2307, 3845], 'maintained': [2309, 2635], 'washing': [2311, 3383], 'fresh': [2315, 3848], 'several': [2317, 3847], 'times': [2318, 3119, 3131], 'during': [2319, 2559, 3073, 3851, 4081, 4102], 'growth.': [2320, 3852], 'liter': [2323], 'culture': [2325], 'reached': [2326], 'absorbance': [2328, 2331], '0.5': [2330, 2453], 'units,': [2332], 'induced': [2335], '400': [2337, 2556, 3937], 'μm': [2338, 2358, 2402, 2499, 2871, 3038, 3087, 3208], 'isopropyl-1-thio-β-d-galactopyranoside.': [2339], '3-h': [2342], 'induction,': [2343], 'washed': [2346, 2442, 2668, 2826, 3117], 'PBS,': [2348, 2670], 'resuspended': [2349, 2394, 2678, 3147, 3394], 'lysis': [2351, 2681], 'buffer': [2352, 2397, 2449, 2468, 2489, 2682, 2833, 2859, 2882, 3385, 3397], '(PBS,': [2353], '1%': [2354, 2456, 3128, 3250, 3391], 'Triton': [2355, 2457], 'X-100,': [2356, 2458], '200': [2357, 2470, 3016, 3141, 3184, 3235, 3252, 4633], 'EDTA,': [2359, 2403, 2500, 2538, 3249], '1': [2360, 2508, 2539, 2697, 2848, 3125, 3387, 3497, 3625], 'mm': [2361, 2399, 2405, 2421, 2451, 2462, 2471, 2491, 2496, 2509, 2526, 2534, 2537, 2540, 2557, 2566, 2684, 2687, 2698, 2811, 2835, 2838, 2849, 2861, 3031, 3034, 3123, 3135, 3138, 3179, 3182, 3242, 3245, 3248, 3938, 4056], 'DTT,': [2362, 2541], '0.1%': [2363, 2501, 2543, 2689, 2692, 2840, 2843, 2867, 2910, 2923, 4087], 'saturated': [2366, 2503, 2546], 'solution': [2367, 2547], 'PMSF': [2369, 2502, 2549], 'isopropyl': [2371, 2505, 2551, 2694, 2845], 'alcohol),': [2372], 'sonicated.': [2374], 'lysate': [2376, 2707], 'spun': [2378, 2413, 2666, 2709], '50,000': [2380], '×': [2381, 2712], 'g': [2382, 2713], '40': [2384], 'min,': [2385, 2716, 3196], 'supernatant': [2387], 'removed,': [2389], 'pellet': [2392, 2411], 'urea': [2396], '(25': [2398, 2490, 2683, 2834, 2860], 'HEPES,': [2400, 2492, 2685, 2836, 2862, 3124, 3136, 3180], 'KCl,': [2406, 2497, 2535, 3032, 3139, 3183], '6': [2407, 3889], 'murea).': [2408], 'urea-extracted': [2410], '15,000': [2415], '×g': [2416], '30': [2418], 'min.': [2419], '3.2': [2420], 'CaCl2': [2422], 'complex': [2426, 2928, 4040, 4065, 4080, 4116, 4212], 'EDTA': [2428, 3105], 'immediately': [2429], 'before': [2430, 4251], 'loading': [2431], 'onto': [2432, 2518, 2814, 3920], '4-ml': [2434], 'Ni2+-nitrilotriacetic': [2435], 'acid-agarose': [2436], 'column': [2437, 2440, 2445, 2522, 2817, 2824, 2903, 3900, 3930, 4241], '(Qiagen).': [2438], '10': [2444, 2715, 3041, 3247, 4356], 'volumes': [2446, 2829, 2855], 'wash': [2448, 2467], '(10': [2450], 'Tris,': [2452, 3243], 'm': [2454, 3388, 3890], 'NaCl,': [2455, 2688, 2839, 3246], '6m': [2459], 'urea,': [2460], '5': [2461, 2854, 2877, 3089, 4327, 4344, 4466, 4650], 'imidazole)': [2463], 'eluted': [2465, 2554, 2875, 2914, 2929, 3935, 4053], 'imidazole.': [2472], 'Purified': [2473], 'combined': [2476], 'approximately': [2479, 4399], '10-fold': [2480, 4507], 'excess': [2481, 4027], 'dialyzed': [2486], 'into': [2487, 2618, 2679, 3000, 3962], 'renaturation': [2488], '15%': [2493, 2531, 2863], 'glycerol,': [2494, 2532, 2864], '150': [2495, 2525, 2533, 2563, 2686, 2865, 2907], 'alcohol,': [2506, 2695, 2846], 'DTT).': [2510], 'Renatured': [2511], 'loaded': [2517, 2813, 3919], 'Mono': [2520, 2899, 3922, 4047, 4194, 4239], 'Q': [2521, 2900, 3923, 4048, 4195, 4240], 'equilibrated': [2523, 2905], 'HGKEDP': [2527, 2921], '(HEPES': [2528], '(pH': [2529], '7.6),': [2530], '0.1': [2536], 'alcohol).': [2552], 'KCl': [2558, 3389, 3939], 'gradient': [2561, 2918], '800': [2565], 'HGKEDP.': [2567], 'decreased': [2576], 'sample': [2580], 'frozen': [2582, 2594, 2673], 'thawed': [2584, 2596], 'due': [2585, 3519], 'precipitation.': [2587], 'this,': [2590], 'all': [2591, 4414, 4518], 'samples': [2592, 4594], 'once.': [2598], 'assist': [2600], 'complete': [2606, 3946], 'protein,': [2608, 3948], 'FLAG-tagged': [2611, 3954], 'N': [2614, 3957], 'terminus': [2615, 3958], 'inserted': [2617], 'mammalian': [2620], 'vector': [2622, 3817], 'pCAGGS': [2623], 'pCMV-FLAG-NELF-E.': [2626], 'Stably': [2627], 'transfected': [2628, 3961], 'S3': [2630, 3964], 'selected': [2633], '500': [2640, 2810, 2837, 3086, 3207], 'Geneticin.': [2642], 'Large,': [2643], 'mycoplasma-free': [2644], 'cultures': [2645], 'Joklik’s': [2649], '5%': [2652], 'calf': [2653], 'serum': [2654, 3144, 3187], 'National': [2657], 'Culture': [2659], 'Center.': [2660], '50': [2661, 3037, 4361, 4645], 'liters': [2662, 2799], 'down,': [2667], 'snap': [2672], 'transportation.': [2675], 'Cells': [2676, 3811], 'isotonic': [2680], 'Nonidet': [2690, 2841, 2868, 2911, 2924, 4088], 'P-40,': [2691, 2842, 2869], 'PMSF-saturated': [2693, 2844], 'DTT)': [2699, 2850], 'homogenized': [2701], 'Dounce': [2704], 'homogenizer.': [2705], '4000': [2711], 'cytoplasmic': [2719, 2773, 2795, 3977, 4001, 4017], 'fraction': [2720, 2725, 2774, 2796, 3662, 3917, 4020, 4586], 'removed.': [2722], 'used': [2727, 2738, 2790, 3970, 4243, 4368, 4546], 'prepare': [2729], 'same': [2736, 4164], 'protocol': [2737, 2975], 'earlier': [2739], 'generation': [2742], 'Kc': [2745], 'cell': [2746], '(29Price': [2749, 3266], 'Sluder': [2751, 3268], 'A.E.': [2752, 3269], 'Greenleaf': [2753, 3270], 'A.L.': [2754, 3271], '1987;': [2758, 3275], '262:': [2759, 3276], '3244-3255Abstract': [2760, 3277], '10%': [2777], 'below.': [2783], 'rest': [2785], 'vitrotranscriptions.': [2793], '12': [2798], 'brought': [2804], 'NaCl': [2807], 'concentration': [2808, 4509, 4513], '3-ml': [2816], 'anti-FLAG': [2819], 'gel.': [2822], '15': [2828], 'high': [2831], 'salt': [2832, 2858, 2881], 'again': [2852], 'low': [2857, 2880], 'mmNaCl,': [2866], 'EDTA).': [2872], 'ml': [2878], 'peptide.': [2887], 'Further': [2888], 'chromatography': [2895, 4045], '1-ml': [2898], 'column.': [2901, 3924], 'mmHGKEDP': [2908], 'P-40': [2912, 4089], '20-ml': [2917], '1m': [2920], 'P-40.': [2925], '360': [2931, 4055], 'mm.': [2932, 3108, 3213], 'DNA': [2934, 2962], 'full': [2938], 'cytomegalovirus': [2939], 'promoter': [2940], 'generated': [2942, 2992, 4675], 'chain': [2945], 'reaction': [2946, 3008], 'pGL2CMV': [2949], 'plasmid': [2950, 3839], '5′-biotinylated': [2953], 'primer': [2954], '1387-base': [2958], 'pair': [2959], 'product.': [2960], 'incubated': [2964, 3026, 3336], 'streptavidin-labeled': [2966], 'superparamagnetic': [2967], 'beads': [2968, 3319], '(Dynabeads': [2969], 'M-280)': [2970], 'according': [2971], 'manufacturer’s': [2974], 'immobilized': [2979, 3113], '548-nt': [2983], 'runoff.': [2984], 'For': [2985, 3004], 'experiment': [2987], 'early': [2988, 3154, 3309, 3330, 3347, 3483, 3608, 3749, 4246, 4312], 'one': [2994], 'large': [2995, 3661], 'reaction,': [2996], 'isolated,': [2997], 'aliquoted': [2999], 'individual': [3001], 'reactions.': [3003], 'final': [3006, 3095, 3860], 'needed,': [3009], '8': [3010], 'μl': [3011, 3022, 3236], 'preincubation': [3013], 'mixture': [3014], 'ng': [3017, 4340, 4345, 4357, 4362, 4462, 4467, 4646, 4651], '1.6': [3021], '20': [3028, 3178], 'mmHEPES,': [3029], '60': [3030, 3137, 3181], '7': [3033, 3212], 'MgCl2,': [3035], 'min': [3042, 4328], 'room': [3044, 3226], 'temperature': [3045, 3227], 'Transcription': [3070], 'initiated': [3072], '30-s': [3075], 'pulse': [3076], 'ATP,': [3081], 'GTP,': [3082], 'UTP': [3084], 'μCi': [3090], '[α-32P]CTP': [3092], 'reaction.': [3097], 'Elongation': [3098], 'halted': [3100, 3375], '25': [3107, 3367], 'Complexes': [3109], 'stringently': [3116], 'HKS': [3121], '(20': [3122, 3134], 'mKCl,': [3126], 'Sarkosyl),': [3129], 'HKB': [3133], 'bovine': [3143, 3186], 'albumin),': [3145], 'HKB.': [3149], 'Extension': [3150], 'carried': [3158], 'out': [3159], '19-μl': [3161], 'reactions': [3162, 4643], 'first': [3164, 3464, 3629], 'mixing': [3165], 'albumin,': [3188], '1.1': [3190], 'units/μl': [3191], 'RNasin': [3192], '(Promega).': [3193], '3–5': [3195], 'resumed': [3199], 'simultaneous': [3202], 'NTPs': [3205], 'MgCl2': [3210], 'Reactions': [3214], 'allowed': [3216, 3854], 'elongate': [3218], 'indicated': [3221], 'stopped': [3230], 'Sarkosyl': [3238, 3392], 'Stop': [3239], 'Solution': [3240], '(100': [3241], 'Sarkosyl,': [3251], 'tRNA).': [3254], 'preparation': [3256], 'analysis': [3258, 4160], '6%': [3260], 'denaturing': [3261], 'previously': [3265, 3733], 'our': [3286, 4282], 'understanding': [3287], 'P-TEFb,': [3292], 'we': [3293, 3441], 'which': [3301, 3501, 3738, 4413], 'could': [3303, 3455, 4255, 4621], 'attachment': [3313], 'magnetic': [3318], 'biotin-streptavidin': [3322], 'linkage': [3323], 'allows': [3324], 'isolation': [3327, 3746], 'preinitiation': [3341], 'CMV': [3345], 'immediate': [3346], 'promoter.': [3348], 'Upon': [3349], 'nucleotides,': [3353], 'including': [3354], 'limiting': [3355], '[α-32P]CTP,': [3356], 'initiates': [3360], 'generates': [3362], 'predominately': [3364], 'less': [3365], 'than': [3366, 4510, 4544, 4687], 'nt': [3368], 'length.': [3370], 'elongating': [3372, 4553], 'stripped': [3377], 'repeatedly': [3382], 'nucleotides.': [3399], 'demonstrated': [3410], 'P-TEFb-dependent': [3413], 'Here': [3440], 'use': [3442], 'determine': [3447, 4612], 'whether': [3448, 4613], 'similar': [3461, 4605], 'manner.': [3462], 'examined': [3465], 'kinetics': [3467, 3718], 'absence': [3488, 4557, 4692], 'diagrammed': [3494], 'Fig.': [3496, 3808, 3989, 4187, 4516], 'Previous': [3499], 'work,': [3500], 'briefly': [3503], 'Introduction,': [3507], 'observed': [3513, 3636, 4230, 4298, 4397], 'reconstituted': [3515], 'exclusively': [3520], 'inhibition': [3523], 'HNE,': [3606, 4300], 'chased': [3612], '0,': [3614, 4333, 4350], '20,': [3615], '30,': [3616], '50,': [3617, 4337, 4630], '80,': [3618], '130,': [3619], '210,': [3620], '340': [3622], 's': [3623], '(Fig.': [3624, 3940, 4049, 4317, 4404, 4472, 4671], 'B).': [3626, 3942, 4051], 'At': [3627, 3650, 4365], 'few': [3630, 4072], 'points,': [3632, 3653], 'little': [3633], 'difference': [3634, 4005], 'potential': [3640, 3658], 'present.': [3649], 'polymerases.': [3665, 4446], 'kinetic': [3667, 3779], 'delay': [3668], 'function': [3671, 3726], 'traveled': [3685], 'distance': [3688], 'down': [3689], 'period': [3698], 'function.': [3702], 'In': [3703, 4289, 4597], 'DRB,': [3707], 'continued': [3709], 'but': [3711, 3880, 4275], 'much': [3714], 'slower': [3715], 'rate.': [3716, 4384], 'timing': [3723], 'seen': [3727, 4007, 4185, 4689], 'here': [3728, 3770, 4547], 'match': [3729], 'what': [3730], 'not': [3743, 4022, 4227, 4287, 4476, 4489, 4528, 4609, 4699, 4711], 'separated': [3744, 4066], 'results': [3768], 'presented': [3769], 'demonstrate': [3771], 'faithfully': [3776], 'appropriate': [3778], 'aspects': [3780], 'control.': [3783], 'subunits': [3786, 3908, 4122], 'Spt5,': [3791], 'expressed': [3794, 3863], 'under': [3802, 3983], '“Experimental': [3803, 3984], 'Procedures”': [3804, 3985], 'summarized': [3806], '2': [3809, 3941], 'reproducibly': [3818], 'caused': [3819], 'slow': [3820], 'growth,': [3821], 'toxic': [3829], 'coli.': [3832], 'resulting': [3834], 'loss': [3835], 'minimized': [3841], 'maintaining': [3843], 'changes': [3850], 'significant': [3856], 'increase': [3857], 'protein.': [3864], 'majority': [3866, 3993], 'produced': [3874], 'insoluble,': [3879], 'solubilized': [3884], 'urea.': [3891, 3905], 'Each': [3892], 'individually': [3895, 4376], 'over': [3897], 'nickel': [3899], 'renatured': [3910], 'dialysis,': [3913], 'soluble': [3916], 'Free': [3925], 'flowed': [3927], 'obtain': [3944], 'subunit,': [3952], 'stably': [3960], 'cells.': [3965], 'M2': [3967], 'antibodies': [3968], 'affinity-purify': [3972], 'NELF-E-containing': [3973], 'fractions': [3980], 'outlined': [3987], '3': [3990, 4050, 4188], 'present': [3998], 'fraction,': [4002], '(data': [4021, 4286, 4527, 4608, 4710], 'shown).': [4023, 4288, 4477, 4529, 4610, 4712], 'apparent': [4030], '52-kDa': [4031], 'material,': [4037], 'KCl.': [4057], 'conditions': [4060, 4411], 'used,': [4061], 'intact': [4063], 'free': [4068], 'other': [4073], 'purification.': [4083], 'throughout': [4090], 'prevented': [4097], 'precipitation': [4098], 'storage': [4105], '−80': [4107], '°C.': [4108], 'Although': [4109], 'reported': [4117], 'five': [4121], 'co-eluting': [4123], 'recent': [4157], 'sequence': [4159], 'gene': [4165], 'encodes': [4166], 'subunits,': [4170], 'NELF-C': [4171], 'NELF-D.': [4173], '2T.': [4174], 'Wada,': [4175], 'Yamaguchi,': [4177], 'Handa,': [4180], 'manuscript': [4181], 'preparation.': [4183], 'As': [4184, 4654], 'B,': [4189], 'resolves': [4197], 'different': [4199], 'differ': [4202, 4271], 'NELF-C/D': [4208, 4273], 'lower': [4215], 'mobility': [4216], 'C-form': [4217], 'eluting': [4218], 'first.': [4219], 'resolution': [4221], 'forms': [4225], 'may': [4226], 'because': [4237], 'stage': [4247], 'composition': [4254, 4274], 'seen.': [4257], 'fractions,': [4266], '31': [4267], '32,': [4269], 'differences': [4278], 'attempt': [4291], 'adding': [4304], '4).': [4318, 4405], 'Transcripts': [4319], 'elongated': [4325, 4438], '6,': [4334], '13,': [4335], '25,': [4336, 4629], '0.6,': [4351], '1.3,': [4352], '2.5,': [4353], '5,': [4354, 4579], 'observable': [4379], 'effects': [4380], 'together,': [4392], 'half': [4400], 'unable': [4408], 'find': [4410], 'affected': [4417, 4434, 4457, 4497], '(see': [4422], '“Discussion”).': [4423], 'saturating': [4425], 'one-third': [4440], 'unaffected': [4445], 'maximal': [4448], 'reduction': [4449], 'occurred': [4459], 'when': [4460], '∼50': [4461], '4': [4473], 'data': [4475], 'Increasing': [4478], 'above': [4485], 'values': [4487], 'did': [4488], 'affect': [4490], 'percentage': [4492], 'higher': [4508, 4543, 4706], 'highest': [4512, 4678], '4,': [4517], 'their': [4521], 'stimulatory': [4531], 'independent': [4534, 4561], 'Concentrations': [4537], 'up': [4540, 4574], '15-fold': [4542], 'those': [4545, 4688], 'purifications': [4562, 4600], 'resulted': [4563], 'preparations': [4566], 'differed': [4572], 'likely': [4582], 'any': [4591, 4694], 'active.': [4596], 'contrast,': [4598], 'separate': [4599], 'very': [4604], 'reversed,': [4623], '(0,': [4628], '100,': [4631], 'ng)': [4634], 'increased,': [4659], '5).': [4672], 'average': [4684], 'slightly': [4685], 'shorter': [4686], 'changed': [4700], 'even': [4705], 'levels': [4707], 'would': [4714], 'expected': [4716], 'if': [4717], 'hig': [4719]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2086949060', 'counts_by_year': [{'year': 2024, 'cited_by_count': 7}, {'year': 2023, 'cited_by_count': 7}, {'year': 2022, 'cited_by_count': 2}, {'year': 2021, 'cited_by_count': 6}, {'year': 2020, 'cited_by_count': 8}, {'year': 2019, 'cited_by_count': 3}, {'year': 2018, 'cited_by_count': 4}, {'year': 2017, 'cited_by_count': 5}, {'year': 2016, 'cited_by_count': 5}, {'year': 2015, 'cited_by_count': 6}, {'year': 2014, 'cited_by_count': 12}, {'year': 2013, 'cited_by_count': 9}, {'year': 2012, 'cited_by_count': 15}], 'updated_date': '2025-01-08T22:20:51.468394', 'created_date': '2016-06-24'}
Publication Information

Basic Information

Access and Citation

AI Researcher Chatbot

Primary Location

Authors

Topics

Keywords

Related Works
