Title: Transcription termination at the end of the tryptophan operon of Escherichia coli
Abstract: The nature of transcription termination at the end of the trp operon of Escherichia coli has been partially characterized. Strains were constructed in which the lac genes were transposed to a chromosomal site near the trp operon. When the lac promoter is deleted from such strains, lac expression depends upon readthrough from the trp operon and can be used to detect mutations which alter normal termination of transcription of the trp operon. It was possible to isolate mutations in the region where transcription of the trp operon normally terminates, thereby allowing substantial readthrough into the lac operon. In addition, a mutation which mapped at or near the rho locus was isolated which allowed efficient readthrough from the trp operon into the lac operon, across a region of about 1800 bases beyond the terminus of the trp operon. These results suggest that rho factor is involved in normal termination of transcription of the trp operon.
Publication Year: 1977
Publication Date: 1977-05-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 46
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