Title: Effects of dietary microbial levan on growth performance, RNA/DNA ratio and some physio-biochemical responses of<i>Labeo rohita</i>(Hamilton) juveniles
Abstract: Aquaculture NutritionVolume 21, Issue 6 p. 892-903 Original Article Effects of dietary microbial levan on growth performance, RNA/DNA ratio and some physio-biochemical responses of Labeo rohita (Hamilton) juveniles S.K. Gupta, Corresponding Author S.K. Gupta Division of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai, India Directorate of Coldwater Fisheries Research, Chhirapani Fish Farm, Champawat, Uttrakhand, IndiaCorrespondence: Sanjay Kumar Gupta, Directorate of Coldwater Fisheries Research, Chhirapani Fish Farm, District – Champawat, Uttrakhand 262523, India. E-mail: [email protected]Search for more papers by this authorA.K. Pal, A.K. Pal Division of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai, IndiaSearch for more papers by this authorN.P. Sahu, N.P. Sahu Division of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai, IndiaSearch for more papers by this authorA.K. Jha, A.K. Jha Division of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai, India Veravel Research Centre of Central Institute of Fisheries Technology (CIFT), Gujrat, IndiaSearch for more papers by this authorS. Kumar, S. Kumar Division of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai, India National Research and Innovation Centre, Research Institute for Fisheries and Aquaculture (NARIC-HAKI), Szarvas, HungarySearch for more papers by this author S.K. Gupta, Corresponding Author S.K. Gupta Division of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai, India Directorate of Coldwater Fisheries Research, Chhirapani Fish Farm, Champawat, Uttrakhand, IndiaCorrespondence: Sanjay Kumar Gupta, Directorate of Coldwater Fisheries Research, Chhirapani Fish Farm, District – Champawat, Uttrakhand 262523, India. E-mail: [email protected]Search for more papers by this authorA.K. Pal, A.K. Pal Division of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai, IndiaSearch for more papers by this authorN.P. Sahu, N.P. Sahu Division of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai, IndiaSearch for more papers by this authorA.K. Jha, A.K. Jha Division of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai, India Veravel Research Centre of Central Institute of Fisheries Technology (CIFT), Gujrat, IndiaSearch for more papers by this authorS. Kumar, S. Kumar Division of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai, India National Research and Innovation Centre, Research Institute for Fisheries and Aquaculture (NARIC-HAKI), Szarvas, HungarySearch for more papers by this author First published: 10 January 2015 https://doi.org/10.1111/anu.12216Citations: 14Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Six isonitrogenous and isocaloric semi-purified diets were prepared with different levels of microbial levan: control (Basal), T1 (Basal + 2.5 g kg−1 diet), T2 (Basal + 5 g kg−1 diet), T3 (Basal + 7.5 g kg−1 diet), T4 (Basal + 10 g kg−1 diet) and T5 (Basal + 12.5 g kg−1 diet), fed to six groups of fish in triplicate tanks. The results of the 60 days feeding trail showed that supplementation of dietary levan significantly affected the weight gain percentage and specific growth rate of the treatment groups fed at 10 g kg−1 or more levan. Lowest feed conversion ratio (FCR) value and highest survival percentage among levan fed groups were observed with 12.5 g kg−1 incorporation (T5) and was comparable with (T4) group. Significant increase in muscle RNA level and RNA/DNA ratio was observed with the increasing dietary levan. Fish fed 12.5 g kg−1 levan had significantly higher protease, amylase and lipase activities compare with the control group. Lowest Aspartate aminotransferase (AST) activity in the liver and muscle was observed in the T5 group fed with highest level of dietary levan. Overall results conclude that dietary microbial levan incorporation at 12.5 g kg−1 could be used as potent dietary prebiotic for the culture of L. rohita juveniles. References Ahilan, B., Shine, G. & Santhanam, R. (2004) Influence of probiotics on the growth and gut microbial load of juvenile goldfish (Carassius auratus). Asian Fish Sci., 17, 271– 278. Akhtar, M.S., Pal, A.K., Sahu, N.P., Ciji, A. & Gupta, S.K. (2012) Effects of dietary pyridoxine on growth and biochemical responses of Labeo rohita fingerlings exposed to endosulfan. Pestic. Biochem. Physiol., 103, 23– 30. AOAC (1990) Official Methods of Analysis of the Association of Official Analytical Chemists, Vol. 1, 14th edn, 1102p. Association of Official Analytical Chemists, Arlington, VA. Avila-Fernandez, A., Galicia-Lagunas, N., Rodrıguez-Alegrıa, M.E., Olvera, C. & Lopez-Munguıa, A. 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