Title: IFN-τ increases PGE2 production and COX-2 gene expression in the bovine endometrium in vitro
Abstract: Prostaglandins (PGs) are well known for their role in reproductive processes. At the time of pregnancy recognition, PGF2α is luteolytic and PGE2 may be antiluteolytic and luteotropic. During the preimplantation period, interferon-tau (IFN-τ) is produced by the conceptus and plays a crucial role in maternal recognition of pregnancy in domestic ruminants. We have demonstrated previously that recombinant bovine and ovine interferon-tau (rbIFN-τ and roIFN-τ) stimulate PGE2 production in epithelial cells, changing the primary PG produced by these cells from F2α to E2. In stromal cells, where PGE2 is the major PG produced, roIFN-τ induced an increase of both types of PGs. The aim of this paper is to identify the possible involvement of cyclooxygenases (COXs) in the modulation of PG production by trophoblastic interferons. Epithelial and stromal cells cultured in vitro were isolated from bovine endometrium and stimulated with increasing doses (1, 10 and 20 μg/ml) of roIFN-τ. PG levels in the culture media were measured by enzyme immunoassays (EIA) and total RNA was extracted from the cells. Northern blot analysis was performed to quantify cyclooxygenase COX-1 (constitutive), COX-2 (inducible) and phospholipase A2 (PLA2) messenger RNA (mRNA) production in response to treatment. The results indicate that roIFN-τ treatment did not affect COX-1 and PLA2 mRNA production in either cell type, whereas COX-2 expression was upregulated in both. The up-regulation of COX-2 transcript was greater in stromal than in epithelial cells. The increase in COX-2 mRNA levels was concurrent with increased production of PGE2 and PGF2α in stromal cells and principally PGE2 in epithelial cells. Furthermore, addition of indomethacin (1 μM) and a specific COX-2 inhibitor (NS-398, 1 μM) blocked the roIFN-τ-stimulation of PG production in both cell types. The mechanism whereby elevated COX-2 expression results in a selective increase of PGE2 in epithelial cells remains to be elucidated. In stromal cells, an increase in COX-2 mRNA levels may explain increased PG production. The overall effect of roIFN-τ in the two cell types is a net increase in PGE2 output.
Publication Year: 1997
Publication Date: 1997-09-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 87
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