Title: SPHEROPLAST INDUCTION AND LYSIS OF BCG STRAINS BY GLYCINE AND LYSOZYME
Abstract: Spheroplast induction and lysis of 6 BCG strains of Mycobacterium tuberculosis by glycine and lysozyme was studied in various media. Spheroplast production was noted in only three strains involving 20% of cell populations. Lysis, as distinct from spheroplast induction, occurred in Dubos medium containing 1.5% glycine and 0.01% lysozyme after 24–48 hours of incubation. Estimation from a standard curve indicated 40 to 70% lysis of the total cell population after 7–10 days of incubation.Similarly, lysis of BCG cells occurred when the inducers, glycine, lysozyme, and lithium chloride, were added to nitrogen-starved cultures grown in Aldridge synthetic medium for 7 to 8 days.
Publication Year: 1966
Publication Date: 1966-04-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 15
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